Induction of proliferation and differentiation of cultured urothelial cells on acellular biomaterials
OBJECTIVE To determine the optimum conditions for the proliferation of urothelial cells, leading to the confluent coverage of large surfaces of biocompatible membranes, and for their terminal differentiation. MATERIALS AND METHODS Porcine and human urothelial cells were cultured on different matrice...
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| Veröffentlicht in: | BJU international 2004-10, Vol.94 (6), p.922-927 |
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| creator | Ram‐Liebig, Gouya Meye, Axel Hakenberg, Oliver W. Haase, Michael Baretton, Gustavo Wirth, Manfred P. |
| description | OBJECTIVE
To determine the optimum conditions for the proliferation of urothelial cells, leading to the confluent coverage of large surfaces of biocompatible membranes, and for their terminal differentiation.
MATERIALS AND METHODS
Porcine and human urothelial cells were cultured on different matrices under different growth conditions. Proliferative activity and the viability of cells were evaluated using fluorescent markers for nuclei and cytoplasm. Growth and differentiation were assessed by histological, histochemical and immunohistochemical methods.
RESULTS
Under fibroblastic induction and supplementation of 5% fetal calf serum (FCS), urothelial cells showed more proliferation than in other conditions tested. Terminal differentiation of superficial cells was achieved by lowering the concentration of FCS to 1% at the air–liquid interface.
CONCLUSIONS
The mitogenic effects of the extracellular matrix content of biological membranes and fibroblastic inductive factors are synergistic with each other, and can compensate for a low FCS concentration and the absence of other additives. Lowering the FCS concentration to 1% inhibits the proliferation of urothelial cells and permits their terminal differentiation. |
| doi_str_mv | 10.1111/j.1464-410X.2004.05061.x |
| format | Article |
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To determine the optimum conditions for the proliferation of urothelial cells, leading to the confluent coverage of large surfaces of biocompatible membranes, and for their terminal differentiation.
MATERIALS AND METHODS
Porcine and human urothelial cells were cultured on different matrices under different growth conditions. Proliferative activity and the viability of cells were evaluated using fluorescent markers for nuclei and cytoplasm. Growth and differentiation were assessed by histological, histochemical and immunohistochemical methods.
RESULTS
Under fibroblastic induction and supplementation of 5% fetal calf serum (FCS), urothelial cells showed more proliferation than in other conditions tested. Terminal differentiation of superficial cells was achieved by lowering the concentration of FCS to 1% at the air–liquid interface.
CONCLUSIONS
The mitogenic effects of the extracellular matrix content of biological membranes and fibroblastic inductive factors are synergistic with each other, and can compensate for a low FCS concentration and the absence of other additives. Lowering the FCS concentration to 1% inhibits the proliferation of urothelial cells and permits their terminal differentiation.</description><identifier>ISSN: 1464-4096</identifier><identifier>EISSN: 1464-410X</identifier><identifier>DOI: 10.1111/j.1464-410X.2004.05061.x</identifier><identifier>PMID: 15476537</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>acellular matrix ; Animals ; Biocompatible Materials ; Biological and medical sciences ; bladder ; Cell Adhesion ; cell culture ; Cell Differentiation ; Cell Division ; Cells, Cultured ; Culture Media ; Humans ; Medical sciences ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Swine ; Technology. Biomaterials. Equipments ; Urinary Bladder - cytology ; urothelium ; Urothelium - cytology</subject><ispartof>BJU international, 2004-10, Vol.94 (6), p.922-927</ispartof><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4611-18f444c8bb2a74ab43fd403c6a97cc8bfba1092d2afcf30a84d80e9c6f87222a3</citedby><cites>FETCH-LOGICAL-c4611-18f444c8bb2a74ab43fd403c6a97cc8bfba1092d2afcf30a84d80e9c6f87222a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1464-410X.2004.05061.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1464-410X.2004.05061.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16196159$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15476537$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ram‐Liebig, Gouya</creatorcontrib><creatorcontrib>Meye, Axel</creatorcontrib><creatorcontrib>Hakenberg, Oliver W.</creatorcontrib><creatorcontrib>Haase, Michael</creatorcontrib><creatorcontrib>Baretton, Gustavo</creatorcontrib><creatorcontrib>Wirth, Manfred P.</creatorcontrib><title>Induction of proliferation and differentiation of cultured urothelial cells on acellular biomaterials</title><title>BJU international</title><addtitle>BJU Int</addtitle><description>OBJECTIVE
To determine the optimum conditions for the proliferation of urothelial cells, leading to the confluent coverage of large surfaces of biocompatible membranes, and for their terminal differentiation.
MATERIALS AND METHODS
Porcine and human urothelial cells were cultured on different matrices under different growth conditions. Proliferative activity and the viability of cells were evaluated using fluorescent markers for nuclei and cytoplasm. Growth and differentiation were assessed by histological, histochemical and immunohistochemical methods.
RESULTS
Under fibroblastic induction and supplementation of 5% fetal calf serum (FCS), urothelial cells showed more proliferation than in other conditions tested. Terminal differentiation of superficial cells was achieved by lowering the concentration of FCS to 1% at the air–liquid interface.
CONCLUSIONS
The mitogenic effects of the extracellular matrix content of biological membranes and fibroblastic inductive factors are synergistic with each other, and can compensate for a low FCS concentration and the absence of other additives. Lowering the FCS concentration to 1% inhibits the proliferation of urothelial cells and permits their terminal differentiation.</description><subject>acellular matrix</subject><subject>Animals</subject><subject>Biocompatible Materials</subject><subject>Biological and medical sciences</subject><subject>bladder</subject><subject>Cell Adhesion</subject><subject>cell culture</subject><subject>Cell Differentiation</subject><subject>Cell Division</subject><subject>Cells, Cultured</subject><subject>Culture Media</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Swine</subject><subject>Technology. Biomaterials. Equipments</subject><subject>Urinary Bladder - cytology</subject><subject>urothelium</subject><subject>Urothelium - cytology</subject><issn>1464-4096</issn><issn>1464-410X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkD1PwzAQhi0EoqXwF1AW2BLsxHGSgQEqPooqsVCJzbo4tnDlJsVORPvvcdqUrni5893zns8vQgHBEfHnbhkRymhICf6MYoxphFPMSLQ5QeO_xukhxwUboQvnlhj7AkvP0YikNGNpko2RnNVVJ1rd1EGjgrVtjFbSwq4AdRVUWvm7rFsNB0h0pu2srILONu2XNBpMIKQxLug1fdYZsEGpmxW00vq2u0Rnygd5NcQJWjw_fUxfw_n7y2z6MA8FZYSEJFeUUpGXZQwZhZImqqI4EQyKTPiyKoHgIq5iUEIlGHJa5VgWgqk8i-MYkgm63c_1H_nupGv5Srt-I6hl0znOWJEWccI8mO9BYRvnrFR8bfUK7JYTzHuL-ZL37vHeSd5bzHcW842XXg9vdOVKVkfh4KkHbgYAnACjLNRCuyPHSMFIWnjufs_9aCO3_16AP74t-iz5Bc2dmhQ</recordid><startdate>200410</startdate><enddate>200410</enddate><creator>Ram‐Liebig, Gouya</creator><creator>Meye, Axel</creator><creator>Hakenberg, Oliver W.</creator><creator>Haase, Michael</creator><creator>Baretton, Gustavo</creator><creator>Wirth, Manfred P.</creator><general>Blackwell Science Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200410</creationdate><title>Induction of proliferation and differentiation of cultured urothelial cells on acellular biomaterials</title><author>Ram‐Liebig, Gouya ; Meye, Axel ; Hakenberg, Oliver W. ; Haase, Michael ; Baretton, Gustavo ; Wirth, Manfred P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4611-18f444c8bb2a74ab43fd403c6a97cc8bfba1092d2afcf30a84d80e9c6f87222a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>acellular matrix</topic><topic>Animals</topic><topic>Biocompatible Materials</topic><topic>Biological and medical sciences</topic><topic>bladder</topic><topic>Cell Adhesion</topic><topic>cell culture</topic><topic>Cell Differentiation</topic><topic>Cell Division</topic><topic>Cells, Cultured</topic><topic>Culture Media</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Swine</topic><topic>Technology. Biomaterials. Equipments</topic><topic>Urinary Bladder - cytology</topic><topic>urothelium</topic><topic>Urothelium - cytology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ram‐Liebig, Gouya</creatorcontrib><creatorcontrib>Meye, Axel</creatorcontrib><creatorcontrib>Hakenberg, Oliver W.</creatorcontrib><creatorcontrib>Haase, Michael</creatorcontrib><creatorcontrib>Baretton, Gustavo</creatorcontrib><creatorcontrib>Wirth, Manfred P.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>BJU international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ram‐Liebig, Gouya</au><au>Meye, Axel</au><au>Hakenberg, Oliver W.</au><au>Haase, Michael</au><au>Baretton, Gustavo</au><au>Wirth, Manfred P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Induction of proliferation and differentiation of cultured urothelial cells on acellular biomaterials</atitle><jtitle>BJU international</jtitle><addtitle>BJU Int</addtitle><date>2004-10</date><risdate>2004</risdate><volume>94</volume><issue>6</issue><spage>922</spage><epage>927</epage><pages>922-927</pages><issn>1464-4096</issn><eissn>1464-410X</eissn><abstract>OBJECTIVE
To determine the optimum conditions for the proliferation of urothelial cells, leading to the confluent coverage of large surfaces of biocompatible membranes, and for their terminal differentiation.
MATERIALS AND METHODS
Porcine and human urothelial cells were cultured on different matrices under different growth conditions. Proliferative activity and the viability of cells were evaluated using fluorescent markers for nuclei and cytoplasm. Growth and differentiation were assessed by histological, histochemical and immunohistochemical methods.
RESULTS
Under fibroblastic induction and supplementation of 5% fetal calf serum (FCS), urothelial cells showed more proliferation than in other conditions tested. Terminal differentiation of superficial cells was achieved by lowering the concentration of FCS to 1% at the air–liquid interface.
CONCLUSIONS
The mitogenic effects of the extracellular matrix content of biological membranes and fibroblastic inductive factors are synergistic with each other, and can compensate for a low FCS concentration and the absence of other additives. Lowering the FCS concentration to 1% inhibits the proliferation of urothelial cells and permits their terminal differentiation.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>15476537</pmid><doi>10.1111/j.1464-410X.2004.05061.x</doi><tpages>6</tpages></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | acellular matrix Animals Biocompatible Materials Biological and medical sciences bladder Cell Adhesion cell culture Cell Differentiation Cell Division Cells, Cultured Culture Media Humans Medical sciences Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Swine Technology. Biomaterials. Equipments Urinary Bladder - cytology urothelium Urothelium - cytology |
| title | Induction of proliferation and differentiation of cultured urothelial cells on acellular biomaterials |
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