In vivo and in vitro sex steroids stimulate seabream gonadotropin-releasing hormone content and release in the protandrous black porgy, Acanthopagrus schlegeli
The objective of the present study was to investigate the regulation of seabream gonadotropin-releasing hormone (sbGnRH) release using in vivo and in vitro approaches in the protandrous black porgy, Acanthopagrus schlegeli. Estradiol-17β (E 2), testosterone (T), and 11-ketotestosterone (11-KT) were...
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| Veröffentlicht in: | General and comparative endocrinology 2004-10, Vol.139 (1), p.12-19 |
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| creator | Lee, Yan-Horn Du, Jin-Lien Shih, Yu-Su Jeng, Shan-Ru Sun, Lian-Tien Chang, Ching-Fong |
| description | The objective of the present study was to investigate the regulation of seabream gonadotropin-releasing hormone (sbGnRH) release using in vivo and in vitro approaches in the protandrous black porgy,
Acanthopagrus schlegeli. Estradiol-17β (E
2), testosterone (T), and 11-ketotestosterone (11-KT) were found to significantly stimulate the increase of sbGnRH levels in pituitary of black porgy after 5–96 h of injection. An in vitro culture system using dispersed brain neurons was also developed to investigate the effects of various steroids on sbGnRH release. Different doses (10
−6–10
−12M) of E
2, T, 11-KT, and cortisol were applied during 6 h experiment. KCl stimulated sbGnRH release at a dose- and time-dependent manner. The concentration of sbGnRH increased 2-fold in the highest dose of KCl treatment compared to the control. Treatments with E
2, T, 11-KT and cortisol significantly stimulated the release of sbGnRH from the cultured brain neurons. The concentration of sbGnRH in medium was increased by 2-, 1.9-, 2.1-, and 4.9-fold when treated with E
2, T, 11-KT, and cortisol, respectively, as compared to the respective control. Cholesterol did not have any stimulatory effects in the release of sbGnRH. The results showed that sex steroids and cortisol had direct effect on brain neuronal cells stimulating the release of sbGnRH. |
| doi_str_mv | 10.1016/j.ygcen.2004.07.010 |
| format | Article |
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Acanthopagrus schlegeli. Estradiol-17β (E
2), testosterone (T), and 11-ketotestosterone (11-KT) were found to significantly stimulate the increase of sbGnRH levels in pituitary of black porgy after 5–96 h of injection. An in vitro culture system using dispersed brain neurons was also developed to investigate the effects of various steroids on sbGnRH release. Different doses (10
−6–10
−12M) of E
2, T, 11-KT, and cortisol were applied during 6 h experiment. KCl stimulated sbGnRH release at a dose- and time-dependent manner. The concentration of sbGnRH increased 2-fold in the highest dose of KCl treatment compared to the control. Treatments with E
2, T, 11-KT and cortisol significantly stimulated the release of sbGnRH from the cultured brain neurons. The concentration of sbGnRH in medium was increased by 2-, 1.9-, 2.1-, and 4.9-fold when treated with E
2, T, 11-KT, and cortisol, respectively, as compared to the respective control. Cholesterol did not have any stimulatory effects in the release of sbGnRH. The results showed that sex steroids and cortisol had direct effect on brain neuronal cells stimulating the release of sbGnRH.</description><identifier>ISSN: 0016-6480</identifier><identifier>EISSN: 1095-6840</identifier><identifier>DOI: 10.1016/j.ygcen.2004.07.010</identifier><identifier>PMID: 15474531</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject><![CDATA[Acanthopagrus schlegeli ; Androgens - administration & dosage ; Androgens - pharmacology ; Animals ; Black porgy ; Brain - cytology ; Brain cell culture ; Cells, Cultured ; Cortisol ; Dose-Response Relationship, Drug ; Estradiol - administration & dosage ; Estradiol - pharmacology ; Fish ; GnRH release ; Gonadal Steroid Hormones - pharmacology ; Gonadotropin-Releasing Hormone - metabolism ; Hydrocortisone - administration & dosage ; Hydrocortisone - pharmacology ; Marine ; Neurons - drug effects ; Neurons - metabolism ; Pituitary ; Pituitary Gland - metabolism ; Potassium Chloride - administration & dosage ; Potassium Chloride - pharmacology ; Sea Bream - metabolism ; Sex steroids ; Testosterone - administration & dosage ; Testosterone - analogs & derivatives ; Testosterone - pharmacology]]></subject><ispartof>General and comparative endocrinology, 2004-10, Vol.139 (1), p.12-19</ispartof><rights>2004 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-8831ff19da346cca4ac6e0d9f80e73d7f6b3a0515be5d72926ede762d41faf1d3</citedby><cites>FETCH-LOGICAL-c386t-8831ff19da346cca4ac6e0d9f80e73d7f6b3a0515be5d72926ede762d41faf1d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0016648004001935$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15474531$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Yan-Horn</creatorcontrib><creatorcontrib>Du, Jin-Lien</creatorcontrib><creatorcontrib>Shih, Yu-Su</creatorcontrib><creatorcontrib>Jeng, Shan-Ru</creatorcontrib><creatorcontrib>Sun, Lian-Tien</creatorcontrib><creatorcontrib>Chang, Ching-Fong</creatorcontrib><title>In vivo and in vitro sex steroids stimulate seabream gonadotropin-releasing hormone content and release in the protandrous black porgy, Acanthopagrus schlegeli</title><title>General and comparative endocrinology</title><addtitle>Gen Comp Endocrinol</addtitle><description>The objective of the present study was to investigate the regulation of seabream gonadotropin-releasing hormone (sbGnRH) release using in vivo and in vitro approaches in the protandrous black porgy,
Acanthopagrus schlegeli. Estradiol-17β (E
2), testosterone (T), and 11-ketotestosterone (11-KT) were found to significantly stimulate the increase of sbGnRH levels in pituitary of black porgy after 5–96 h of injection. An in vitro culture system using dispersed brain neurons was also developed to investigate the effects of various steroids on sbGnRH release. Different doses (10
−6–10
−12M) of E
2, T, 11-KT, and cortisol were applied during 6 h experiment. KCl stimulated sbGnRH release at a dose- and time-dependent manner. The concentration of sbGnRH increased 2-fold in the highest dose of KCl treatment compared to the control. Treatments with E
2, T, 11-KT and cortisol significantly stimulated the release of sbGnRH from the cultured brain neurons. The concentration of sbGnRH in medium was increased by 2-, 1.9-, 2.1-, and 4.9-fold when treated with E
2, T, 11-KT, and cortisol, respectively, as compared to the respective control. Cholesterol did not have any stimulatory effects in the release of sbGnRH. The results showed that sex steroids and cortisol had direct effect on brain neuronal cells stimulating the release of sbGnRH.</description><subject>Acanthopagrus schlegeli</subject><subject>Androgens - administration & dosage</subject><subject>Androgens - pharmacology</subject><subject>Animals</subject><subject>Black porgy</subject><subject>Brain - cytology</subject><subject>Brain cell culture</subject><subject>Cells, Cultured</subject><subject>Cortisol</subject><subject>Dose-Response Relationship, Drug</subject><subject>Estradiol - administration & dosage</subject><subject>Estradiol - pharmacology</subject><subject>Fish</subject><subject>GnRH release</subject><subject>Gonadal Steroid Hormones - pharmacology</subject><subject>Gonadotropin-Releasing Hormone - metabolism</subject><subject>Hydrocortisone - administration & dosage</subject><subject>Hydrocortisone - pharmacology</subject><subject>Marine</subject><subject>Neurons - drug effects</subject><subject>Neurons - metabolism</subject><subject>Pituitary</subject><subject>Pituitary Gland - metabolism</subject><subject>Potassium Chloride - administration & dosage</subject><subject>Potassium Chloride - pharmacology</subject><subject>Sea Bream - metabolism</subject><subject>Sex steroids</subject><subject>Testosterone - administration & dosage</subject><subject>Testosterone - analogs & derivatives</subject><subject>Testosterone - pharmacology</subject><issn>0016-6480</issn><issn>1095-6840</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcGO1DAMhiMEYmcXngAJ5cSJFqdp0_bAYbWCZaWVuMA5ShO3k6FNSpIZMU_Dq5LZGYkbnGzZn39b_gl5w6BkwMSHXXmcNLqyAqhLaEtg8IxsGPRNIboanpMNZKwQdQdX5DrGHQA0XLCX5Io1dVs3nG3I7wdHD_bgqXKG2lOegqcRf9GYMHhrYk7ssp9VwlxWQ0C10Mk7ZXwmV-uKgDOqaN1Etz4s3iHV3iV06Unz3MWTdtoiXYNPuRz8PtJhVvoHXX2Yju_prVYubf2qppBbUW9nnHC2r8iLUc0RX1_iDfn--dO3uy_F49f7h7vbx0LzTqSi6zgbR9YbxWuhtaqVFgimHzvAlpt2FANX0LBmwMa0VV8JNNiKytRsVCMz_Ia8O-vmA3_uMSa52KhxnpXDfKsUom-6tmr-C7K-ZsBZlUF-BnXwMQYc5RrsosJRMpAnA-VOPhkoTwZKaGU2ME-9vcjvhwXN35mLYxn4eAYwf-NgMcioLTqNxgbUSRpv_7ngDwBBsh4</recordid><startdate>20041001</startdate><enddate>20041001</enddate><creator>Lee, Yan-Horn</creator><creator>Du, Jin-Lien</creator><creator>Shih, Yu-Su</creator><creator>Jeng, Shan-Ru</creator><creator>Sun, Lian-Tien</creator><creator>Chang, Ching-Fong</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>20041001</creationdate><title>In vivo and in vitro sex steroids stimulate seabream gonadotropin-releasing hormone content and release in the protandrous black porgy, Acanthopagrus schlegeli</title><author>Lee, Yan-Horn ; Du, Jin-Lien ; Shih, Yu-Su ; Jeng, Shan-Ru ; Sun, Lian-Tien ; Chang, Ching-Fong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-8831ff19da346cca4ac6e0d9f80e73d7f6b3a0515be5d72926ede762d41faf1d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Acanthopagrus schlegeli</topic><topic>Androgens - administration & dosage</topic><topic>Androgens - pharmacology</topic><topic>Animals</topic><topic>Black porgy</topic><topic>Brain - cytology</topic><topic>Brain cell culture</topic><topic>Cells, Cultured</topic><topic>Cortisol</topic><topic>Dose-Response Relationship, Drug</topic><topic>Estradiol - administration & dosage</topic><topic>Estradiol - pharmacology</topic><topic>Fish</topic><topic>GnRH release</topic><topic>Gonadal Steroid Hormones - pharmacology</topic><topic>Gonadotropin-Releasing Hormone - metabolism</topic><topic>Hydrocortisone - administration & dosage</topic><topic>Hydrocortisone - pharmacology</topic><topic>Marine</topic><topic>Neurons - drug effects</topic><topic>Neurons - metabolism</topic><topic>Pituitary</topic><topic>Pituitary Gland - metabolism</topic><topic>Potassium Chloride - administration & dosage</topic><topic>Potassium Chloride - pharmacology</topic><topic>Sea Bream - metabolism</topic><topic>Sex steroids</topic><topic>Testosterone - administration & dosage</topic><topic>Testosterone - analogs & derivatives</topic><topic>Testosterone - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Yan-Horn</creatorcontrib><creatorcontrib>Du, Jin-Lien</creatorcontrib><creatorcontrib>Shih, Yu-Su</creatorcontrib><creatorcontrib>Jeng, Shan-Ru</creatorcontrib><creatorcontrib>Sun, Lian-Tien</creatorcontrib><creatorcontrib>Chang, Ching-Fong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>General and comparative endocrinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Yan-Horn</au><au>Du, Jin-Lien</au><au>Shih, Yu-Su</au><au>Jeng, Shan-Ru</au><au>Sun, Lian-Tien</au><au>Chang, Ching-Fong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vivo and in vitro sex steroids stimulate seabream gonadotropin-releasing hormone content and release in the protandrous black porgy, Acanthopagrus schlegeli</atitle><jtitle>General and comparative endocrinology</jtitle><addtitle>Gen Comp Endocrinol</addtitle><date>2004-10-01</date><risdate>2004</risdate><volume>139</volume><issue>1</issue><spage>12</spage><epage>19</epage><pages>12-19</pages><issn>0016-6480</issn><eissn>1095-6840</eissn><abstract>The objective of the present study was to investigate the regulation of seabream gonadotropin-releasing hormone (sbGnRH) release using in vivo and in vitro approaches in the protandrous black porgy,
Acanthopagrus schlegeli. Estradiol-17β (E
2), testosterone (T), and 11-ketotestosterone (11-KT) were found to significantly stimulate the increase of sbGnRH levels in pituitary of black porgy after 5–96 h of injection. An in vitro culture system using dispersed brain neurons was also developed to investigate the effects of various steroids on sbGnRH release. Different doses (10
−6–10
−12M) of E
2, T, 11-KT, and cortisol were applied during 6 h experiment. KCl stimulated sbGnRH release at a dose- and time-dependent manner. The concentration of sbGnRH increased 2-fold in the highest dose of KCl treatment compared to the control. Treatments with E
2, T, 11-KT and cortisol significantly stimulated the release of sbGnRH from the cultured brain neurons. The concentration of sbGnRH in medium was increased by 2-, 1.9-, 2.1-, and 4.9-fold when treated with E
2, T, 11-KT, and cortisol, respectively, as compared to the respective control. Cholesterol did not have any stimulatory effects in the release of sbGnRH. The results showed that sex steroids and cortisol had direct effect on brain neuronal cells stimulating the release of sbGnRH.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>15474531</pmid><doi>10.1016/j.ygcen.2004.07.010</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | General and comparative endocrinology, 2004-10, Vol.139 (1), p.12-19 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Acanthopagrus schlegeli Androgens - administration & dosage Androgens - pharmacology Animals Black porgy Brain - cytology Brain cell culture Cells, Cultured Cortisol Dose-Response Relationship, Drug Estradiol - administration & dosage Estradiol - pharmacology Fish GnRH release Gonadal Steroid Hormones - pharmacology Gonadotropin-Releasing Hormone - metabolism Hydrocortisone - administration & dosage Hydrocortisone - pharmacology Marine Neurons - drug effects Neurons - metabolism Pituitary Pituitary Gland - metabolism Potassium Chloride - administration & dosage Potassium Chloride - pharmacology Sea Bream - metabolism Sex steroids Testosterone - administration & dosage Testosterone - analogs & derivatives Testosterone - pharmacology |
| title | In vivo and in vitro sex steroids stimulate seabream gonadotropin-releasing hormone content and release in the protandrous black porgy, Acanthopagrus schlegeli |
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