Single-particle sizing from light scattering by spectral decomposition
A Fourier transform was applied to size an individual spherical particle from an angular light-scattering pattern. The position of the peak in the amplitude spectrum has a strong correlation with the particle size. A linear equation retrieved from regression analysis of theoretically simulated patte...
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| Veröffentlicht in: | Applied Optics 2004-09, Vol.43 (26), p.5110-5115 |
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| container_issue | 26 |
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| container_title | Applied Optics |
| container_volume | 43 |
| creator | Semyanov, Konstantin A Tarasov, Peter A Zharinov, Alexey E Chernyshev, Andrei V Hoekstra, Alfons G Maltsev, Valeri P |
| description | A Fourier transform was applied to size an individual spherical particle from an angular light-scattering pattern. The position of the peak in the amplitude spectrum has a strong correlation with the particle size. A linear equation retrieved from regression analysis of theoretically simulated patterns provides a relation between the particle size and the location of the amplitude spectrum's peak. The equation can be successfully applied to characterize particles of size parameters that range from 8 to 180 (corresponding to particle sizes that range from 1.2 to 27.2 microm at a wavelength of 0.633 microm). The precision of particle sizing depends on the refractive index and reaches a value of 60 nm within refractive-index region from 1.35 to 1.70. We have analyzed four samples of polystyrene microspheres with mean diameters of 1.9, 2.6, 3.0, and 4.2 microm and a sample of isovolumetrically sphered erythrocytes with a scanning flow cytometer to compare the accuracy of our new method with that of others. |
| doi_str_mv | 10.1364/AO.43.005110 |
| format | Article |
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The position of the peak in the amplitude spectrum has a strong correlation with the particle size. A linear equation retrieved from regression analysis of theoretically simulated patterns provides a relation between the particle size and the location of the amplitude spectrum's peak. The equation can be successfully applied to characterize particles of size parameters that range from 8 to 180 (corresponding to particle sizes that range from 1.2 to 27.2 microm at a wavelength of 0.633 microm). The precision of particle sizing depends on the refractive index and reaches a value of 60 nm within refractive-index region from 1.35 to 1.70. 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The position of the peak in the amplitude spectrum has a strong correlation with the particle size. A linear equation retrieved from regression analysis of theoretically simulated patterns provides a relation between the particle size and the location of the amplitude spectrum's peak. The equation can be successfully applied to characterize particles of size parameters that range from 8 to 180 (corresponding to particle sizes that range from 1.2 to 27.2 microm at a wavelength of 0.633 microm). The precision of particle sizing depends on the refractive index and reaches a value of 60 nm within refractive-index region from 1.35 to 1.70. 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| identifier | ISSN: 1559-128X |
| ispartof | Applied Optics, 2004-09, Vol.43 (26), p.5110-5115 |
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| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection; Optica Publishing Group Journals |
| subjects | Algorithms Cell Size Erythrocytes - cytology Flow Cytometry - methods Image Interpretation, Computer-Assisted - methods Laser Scanning Cytometry - methods Particle Size Scattering, Radiation |
| title | Single-particle sizing from light scattering by spectral decomposition |
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