Polysaccharides of Ganoderma lucidum alter cell immunophenotypic expression and enhance CD56 + NK-cell cytotoxicity in cord blood
Human umbilical cord blood MNCs were treated with polysaccharides of Ganoderma lucidum F3 and cultured for 7 days. Analysis of various subsets of UCB mononuclear cells revealed relative increments of macrophage, dendritic cells, and natural killer cells detected by flow cytometry after the culture p...
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| Veröffentlicht in: | Bioorganic & medicinal chemistry 2004-11, Vol.12 (21), p.5603-5609 |
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| creator | Chien, Chichen Michael Cheng, Jing-Long Chang, Wen-Teish Tien, Ming-Hsun Tsao, Chien-Ming Chang, Yung-Han Chang, Hwan-You Hsieh, Jung-Feng Wong, Chi-Huey Chen, Shui-Tein |
| description | Human umbilical cord blood MNCs were treated with polysaccharides of
Ganoderma lucidum F3 and cultured for 7
days. Analysis of various subsets of UCB mononuclear cells revealed relative increments of macrophage, dendritic cells, and natural killer cells detected by flow cytometry after the culture period.
In our previous study, a fucose-containing glycoprotein fraction (F3), isolated from the water-soluble extracts of
Ganoderma lucidum, was shown to stimulate mice spleen cell proliferation and cytokine expression. We now further investigate the effect of F3 on the immunophenotypic expression in mononuclear cells (MNCs). When human umbilical cord blood (hUCB) MNCs were treated with F3 (10–100
μg/mL) for 7
days, the population of CD14
+CD26
+ monocyte/macrophage, CD83
+CD1a
+ dendritic cells, and CD16
+CD56
+ NK-cells were 2.9, 2.3, and 1.5 times higher than those of the untreated controls (
p
<
0.05). B-cell population has no significant change. T cell growth was, however, slightly inhibited and CD3 marker expression decreased ∼20% in the presence of higher concentrations of F3 (100
μg/mL).
We also found that F3 is not harmful to human cells in vitro, and after F3 treatment, NK-cell-mediated cytotoxicity was significantly enhanced by 31.7% (
p
<
0.01) at effector/target cell ratio (
E/
T) 20:1, but was not altered at
E/
T 5:1. |
| doi_str_mv | 10.1016/j.bmc.2004.08.004 |
| format | Article |
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Ganoderma lucidum F3 and cultured for 7
days. Analysis of various subsets of UCB mononuclear cells revealed relative increments of macrophage, dendritic cells, and natural killer cells detected by flow cytometry after the culture period.
In our previous study, a fucose-containing glycoprotein fraction (F3), isolated from the water-soluble extracts of
Ganoderma lucidum, was shown to stimulate mice spleen cell proliferation and cytokine expression. We now further investigate the effect of F3 on the immunophenotypic expression in mononuclear cells (MNCs). When human umbilical cord blood (hUCB) MNCs were treated with F3 (10–100
μg/mL) for 7
days, the population of CD14
+CD26
+ monocyte/macrophage, CD83
+CD1a
+ dendritic cells, and CD16
+CD56
+ NK-cells were 2.9, 2.3, and 1.5 times higher than those of the untreated controls (
p
<
0.05). B-cell population has no significant change. T cell growth was, however, slightly inhibited and CD3 marker expression decreased ∼20% in the presence of higher concentrations of F3 (100
μg/mL).
We also found that F3 is not harmful to human cells in vitro, and after F3 treatment, NK-cell-mediated cytotoxicity was significantly enhanced by 31.7% (
p
<
0.01) at effector/target cell ratio (
E/
T) 20:1, but was not altered at
E/
T 5:1.</description><identifier>ISSN: 0968-0896</identifier><identifier>EISSN: 1464-3391</identifier><identifier>DOI: 10.1016/j.bmc.2004.08.004</identifier><identifier>PMID: 15465338</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Alamar blue ; Biological and medical sciences ; CD56 Antigen - biosynthesis ; Cell Line, Tumor ; Cell Proliferation - drug effects ; Cytotoxicity, Immunologic - drug effects ; Fetal Blood - cytology ; Fetal Blood - drug effects ; Fetal Blood - immunology ; Flow cytometry ; Fluorescent labeled ; Ganoderma lucidum ; General pharmacology ; Humans ; Immunologic Factors - isolation & purification ; Immunologic Factors - pharmacology ; Immunophenotyping ; Killer Cells, Natural - drug effects ; Killer Cells, Natural - immunology ; Killer Cells, Natural - metabolism ; Lymphocyte Activation - drug effects ; Medical sciences ; Natural killer cells ; Pharmacognosy. Homeopathy. Health food ; Pharmacology. Drug treatments ; Polysaccharides - isolation & purification ; Polysaccharides - pharmacology ; Reishi ; Reishi polysaccharides ; Umbilical cord blood</subject><ispartof>Bioorganic & medicinal chemistry, 2004-11, Vol.12 (21), p.5603-5609</ispartof><rights>2004 Elsevier Ltd</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c488t-a6612a8add3a08432f5403ea692b524c58dc344e14fbff950c6c1e35a2092b603</citedby><cites>FETCH-LOGICAL-c488t-a6612a8add3a08432f5403ea692b524c58dc344e14fbff950c6c1e35a2092b603</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bmc.2004.08.004$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16172236$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15465338$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chien, Chichen Michael</creatorcontrib><creatorcontrib>Cheng, Jing-Long</creatorcontrib><creatorcontrib>Chang, Wen-Teish</creatorcontrib><creatorcontrib>Tien, Ming-Hsun</creatorcontrib><creatorcontrib>Tsao, Chien-Ming</creatorcontrib><creatorcontrib>Chang, Yung-Han</creatorcontrib><creatorcontrib>Chang, Hwan-You</creatorcontrib><creatorcontrib>Hsieh, Jung-Feng</creatorcontrib><creatorcontrib>Wong, Chi-Huey</creatorcontrib><creatorcontrib>Chen, Shui-Tein</creatorcontrib><title>Polysaccharides of Ganoderma lucidum alter cell immunophenotypic expression and enhance CD56 + NK-cell cytotoxicity in cord blood</title><title>Bioorganic & medicinal chemistry</title><addtitle>Bioorg Med Chem</addtitle><description>Human umbilical cord blood MNCs were treated with polysaccharides of
Ganoderma lucidum F3 and cultured for 7
days. Analysis of various subsets of UCB mononuclear cells revealed relative increments of macrophage, dendritic cells, and natural killer cells detected by flow cytometry after the culture period.
In our previous study, a fucose-containing glycoprotein fraction (F3), isolated from the water-soluble extracts of
Ganoderma lucidum, was shown to stimulate mice spleen cell proliferation and cytokine expression. We now further investigate the effect of F3 on the immunophenotypic expression in mononuclear cells (MNCs). When human umbilical cord blood (hUCB) MNCs were treated with F3 (10–100
μg/mL) for 7
days, the population of CD14
+CD26
+ monocyte/macrophage, CD83
+CD1a
+ dendritic cells, and CD16
+CD56
+ NK-cells were 2.9, 2.3, and 1.5 times higher than those of the untreated controls (
p
<
0.05). B-cell population has no significant change. T cell growth was, however, slightly inhibited and CD3 marker expression decreased ∼20% in the presence of higher concentrations of F3 (100
μg/mL).
We also found that F3 is not harmful to human cells in vitro, and after F3 treatment, NK-cell-mediated cytotoxicity was significantly enhanced by 31.7% (
p
<
0.01) at effector/target cell ratio (
E/
T) 20:1, but was not altered at
E/
T 5:1.</description><subject>Alamar blue</subject><subject>Biological and medical sciences</subject><subject>CD56 Antigen - biosynthesis</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation - drug effects</subject><subject>Cytotoxicity, Immunologic - drug effects</subject><subject>Fetal Blood - cytology</subject><subject>Fetal Blood - drug effects</subject><subject>Fetal Blood - immunology</subject><subject>Flow cytometry</subject><subject>Fluorescent labeled</subject><subject>Ganoderma lucidum</subject><subject>General pharmacology</subject><subject>Humans</subject><subject>Immunologic Factors - isolation & purification</subject><subject>Immunologic Factors - pharmacology</subject><subject>Immunophenotyping</subject><subject>Killer Cells, Natural - drug effects</subject><subject>Killer Cells, Natural - immunology</subject><subject>Killer Cells, Natural - metabolism</subject><subject>Lymphocyte Activation - drug effects</subject><subject>Medical sciences</subject><subject>Natural killer cells</subject><subject>Pharmacognosy. Homeopathy. Health food</subject><subject>Pharmacology. Drug treatments</subject><subject>Polysaccharides - isolation & purification</subject><subject>Polysaccharides - pharmacology</subject><subject>Reishi</subject><subject>Reishi polysaccharides</subject><subject>Umbilical cord blood</subject><issn>0968-0896</issn><issn>1464-3391</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFu1DAURS0EotPCB7BB3sAGJbVjx3XECg20VFTAAtaW8_yi8SiJg52gZtk_x8OM1F1Xd3Pu1dUh5A1nJWdcXe7LdoCyYkyWTJc5npENl0oWQjT8OdmwRumC6UadkfOU9oyxSjb8JTnjtVS1EHpDHn6Gfk0WYGejd5ho6OiNHYPDOFjaL-DdMlDbzxgpYN9TPwzLGKYdjmFeJw8U76eIKfkwUjs6iuPOjoB0-7lW9AP9_q34X4N1DnO49-DnlfqRQoiOtn0I7hV50dk-4etTXpDf119-bb8Wdz9ubref7gqQWs-FVYpXVlvnhGVaiqqrJRNoVVO1dSWh1g6ElMhl13ZdUzNQwFHUtmKZUExckPfH3SmGPwum2Qw-Hb7ZEcOSjFKNVIJfZZAfQYghpYidmaIfbFwNZ-bg3exN9m4O3g3TJkfuvD2NL-2A7rFxEp2BdyfAJrB9F7Mknx45xa-qSqjMfTxymFX89RhNAo9ZqPMRYTYu-Cdu_AOEqKE3</recordid><startdate>20041101</startdate><enddate>20041101</enddate><creator>Chien, Chichen Michael</creator><creator>Cheng, Jing-Long</creator><creator>Chang, Wen-Teish</creator><creator>Tien, Ming-Hsun</creator><creator>Tsao, Chien-Ming</creator><creator>Chang, Yung-Han</creator><creator>Chang, Hwan-You</creator><creator>Hsieh, Jung-Feng</creator><creator>Wong, Chi-Huey</creator><creator>Chen, Shui-Tein</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20041101</creationdate><title>Polysaccharides of Ganoderma lucidum alter cell immunophenotypic expression and enhance CD56 + NK-cell cytotoxicity in cord blood</title><author>Chien, Chichen Michael ; Cheng, Jing-Long ; Chang, Wen-Teish ; Tien, Ming-Hsun ; Tsao, Chien-Ming ; Chang, Yung-Han ; Chang, Hwan-You ; Hsieh, Jung-Feng ; Wong, Chi-Huey ; Chen, Shui-Tein</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c488t-a6612a8add3a08432f5403ea692b524c58dc344e14fbff950c6c1e35a2092b603</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Alamar blue</topic><topic>Biological and medical sciences</topic><topic>CD56 Antigen - biosynthesis</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation - drug effects</topic><topic>Cytotoxicity, Immunologic - drug effects</topic><topic>Fetal Blood - cytology</topic><topic>Fetal Blood - drug effects</topic><topic>Fetal Blood - immunology</topic><topic>Flow cytometry</topic><topic>Fluorescent labeled</topic><topic>Ganoderma lucidum</topic><topic>General pharmacology</topic><topic>Humans</topic><topic>Immunologic Factors - isolation & purification</topic><topic>Immunologic Factors - pharmacology</topic><topic>Immunophenotyping</topic><topic>Killer Cells, Natural - drug effects</topic><topic>Killer Cells, Natural - immunology</topic><topic>Killer Cells, Natural - metabolism</topic><topic>Lymphocyte Activation - drug effects</topic><topic>Medical sciences</topic><topic>Natural killer cells</topic><topic>Pharmacognosy. Homeopathy. Health food</topic><topic>Pharmacology. Drug treatments</topic><topic>Polysaccharides - isolation & purification</topic><topic>Polysaccharides - pharmacology</topic><topic>Reishi</topic><topic>Reishi polysaccharides</topic><topic>Umbilical cord blood</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chien, Chichen Michael</creatorcontrib><creatorcontrib>Cheng, Jing-Long</creatorcontrib><creatorcontrib>Chang, Wen-Teish</creatorcontrib><creatorcontrib>Tien, Ming-Hsun</creatorcontrib><creatorcontrib>Tsao, Chien-Ming</creatorcontrib><creatorcontrib>Chang, Yung-Han</creatorcontrib><creatorcontrib>Chang, Hwan-You</creatorcontrib><creatorcontrib>Hsieh, Jung-Feng</creatorcontrib><creatorcontrib>Wong, Chi-Huey</creatorcontrib><creatorcontrib>Chen, Shui-Tein</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Bioorganic & medicinal chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chien, Chichen Michael</au><au>Cheng, Jing-Long</au><au>Chang, Wen-Teish</au><au>Tien, Ming-Hsun</au><au>Tsao, Chien-Ming</au><au>Chang, Yung-Han</au><au>Chang, Hwan-You</au><au>Hsieh, Jung-Feng</au><au>Wong, Chi-Huey</au><au>Chen, Shui-Tein</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Polysaccharides of Ganoderma lucidum alter cell immunophenotypic expression and enhance CD56 + NK-cell cytotoxicity in cord blood</atitle><jtitle>Bioorganic & medicinal chemistry</jtitle><addtitle>Bioorg Med Chem</addtitle><date>2004-11-01</date><risdate>2004</risdate><volume>12</volume><issue>21</issue><spage>5603</spage><epage>5609</epage><pages>5603-5609</pages><issn>0968-0896</issn><eissn>1464-3391</eissn><abstract>Human umbilical cord blood MNCs were treated with polysaccharides of
Ganoderma lucidum F3 and cultured for 7
days. Analysis of various subsets of UCB mononuclear cells revealed relative increments of macrophage, dendritic cells, and natural killer cells detected by flow cytometry after the culture period.
In our previous study, a fucose-containing glycoprotein fraction (F3), isolated from the water-soluble extracts of
Ganoderma lucidum, was shown to stimulate mice spleen cell proliferation and cytokine expression. We now further investigate the effect of F3 on the immunophenotypic expression in mononuclear cells (MNCs). When human umbilical cord blood (hUCB) MNCs were treated with F3 (10–100
μg/mL) for 7
days, the population of CD14
+CD26
+ monocyte/macrophage, CD83
+CD1a
+ dendritic cells, and CD16
+CD56
+ NK-cells were 2.9, 2.3, and 1.5 times higher than those of the untreated controls (
p
<
0.05). B-cell population has no significant change. T cell growth was, however, slightly inhibited and CD3 marker expression decreased ∼20% in the presence of higher concentrations of F3 (100
μg/mL).
We also found that F3 is not harmful to human cells in vitro, and after F3 treatment, NK-cell-mediated cytotoxicity was significantly enhanced by 31.7% (
p
<
0.01) at effector/target cell ratio (
E/
T) 20:1, but was not altered at
E/
T 5:1.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>15465338</pmid><doi>10.1016/j.bmc.2004.08.004</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | Bioorganic & medicinal chemistry, 2004-11, Vol.12 (21), p.5603-5609 |
| issn | 0968-0896 1464-3391 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Alamar blue Biological and medical sciences CD56 Antigen - biosynthesis Cell Line, Tumor Cell Proliferation - drug effects Cytotoxicity, Immunologic - drug effects Fetal Blood - cytology Fetal Blood - drug effects Fetal Blood - immunology Flow cytometry Fluorescent labeled Ganoderma lucidum General pharmacology Humans Immunologic Factors - isolation & purification Immunologic Factors - pharmacology Immunophenotyping Killer Cells, Natural - drug effects Killer Cells, Natural - immunology Killer Cells, Natural - metabolism Lymphocyte Activation - drug effects Medical sciences Natural killer cells Pharmacognosy. Homeopathy. Health food Pharmacology. Drug treatments Polysaccharides - isolation & purification Polysaccharides - pharmacology Reishi Reishi polysaccharides Umbilical cord blood |
| title | Polysaccharides of Ganoderma lucidum alter cell immunophenotypic expression and enhance CD56 + NK-cell cytotoxicity in cord blood |
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