Cloning and overexpression of the old yellow enzyme gene of Candida macedoniensis, and its application to the production of a chiral compound

The gene encoding old yellow enzyme (OYE), which catalyzes the conversion of ketoisophorone (KIP; 2,6,6-trimethyl-2-cyclohexen-1,4-dione) to (6 R)-levodione (2,2,6-trimethylcyclohexane-1,4-dione), of Candida macedoniensis was cloned and sequenced. A 1212 bp nucleotide fragment ( oye) was confirmed t...

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Veröffentlicht in:	Journal of biotechnology 2004-10, Vol.114 (1), p.1-9
Hauptverfasser:	Kataoka, Michihiko, Kotaka, Atsushi, Thiwthong, Rungruedee, Wada, Masaru, Nakamori, Shigeru, Shimizu, Sakayu
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence amino acid sequences Asymmetric hydrogenation Biological and medical sciences Biotechnology Candida - enzymology Candida - genetics Candida macedoniensis Cloning, Molecular - methods Cyclohexanones - metabolism Enzyme Activation enzyme activity Escherichia coli - enzymology Escherichia coli - genetics Fundamental and applied biological sciences. Psychology gene overexpression genes Glucose 1-Dehydrogenase - genetics Glucose 1-Dehydrogenase - metabolism Isomerism molecular cloning Molecular Sequence Data Molecular Weight NADPH dehydrogenase NADPH Dehydrogenase - chemistry NADPH Dehydrogenase - genetics NADPH Dehydrogenase - metabolism nucleotide sequences Old yellow enzyme oxidoreductases Protein Engineering - methods Sequence Homology, Amino Acid
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