CD4 and CD8 enumeration for HIV monitoring in resource‐constrained settings

Background: We developed a volumetric single platform image cytometer (SP ICM) that is dedicated to count CD4+ and CD8+ T lymphocytes for HIV monitoring in resource‐constrained settings. The instrument was designed to be low‐cost, yet reliable, easy‐to‐use, and robust. Methods: Whole blood is incuba...

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Veröffentlicht in:	Cytometry. Part B, Clinical cytometry Clinical cytometry, 2009-03, Vol.76B (2), p.118-126
Hauptverfasser:	Li, Xiao, Breukers, Christian, Ymeti, Aurel, Lunter, Björn, Terstappen, Leon W. M. M., Greve, Jan
Format:	Artikel
Sprache:	eng
Schlagworte:	CD4 and CD8 enumeration CD4 Antigens - analysis CD4 Antigens - metabolism CD4 Lymphocyte Count - methods CD4-CD8 Ratio - methods CD8 Antigens - analysis CD8 Antigens - metabolism Flow Cytometry - economics Flow Cytometry - methods Fluorescent Dyes HIV Infections - blood HIV Infections - diagnosis HIV Infections - immunology HIV monitoring Human immunodeficiency virus Humans image cytometer Image Cytometry - economics Image Cytometry - instrumentation Image Cytometry - methods immunofluorescent labeling immunomagnetic separation Immunomagnetic Separation - economics Immunomagnetic Separation - instrumentation Immunomagnetic Separation - methods Microscopy, Fluorescence - methods Monitoring, Immunologic - economics Monitoring, Immunologic - instrumentation Monitoring, Immunologic - methods point‐of‐care Predictive Value of Tests single platform T-Lymphocytes - immunology T-Lymphocytes - virology
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