CD4 and CD8 enumeration for HIV monitoring in resource‐constrained settings
Background: We developed a volumetric single platform image cytometer (SP ICM) that is dedicated to count CD4+ and CD8+ T lymphocytes for HIV monitoring in resource‐constrained settings. The instrument was designed to be low‐cost, yet reliable, easy‐to‐use, and robust. Methods: Whole blood is incuba...
Gespeichert in:
| Veröffentlicht in: | Cytometry. Part B, Clinical cytometry Clinical cytometry, 2009-03, Vol.76B (2), p.118-126 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 126 |
|---|---|
| container_issue | 2 |
| container_start_page | 118 |
| container_title | Cytometry. Part B, Clinical cytometry |
| container_volume | 76B |
| creator | Li, Xiao Breukers, Christian Ymeti, Aurel Lunter, Björn Terstappen, Leon W. M. M. Greve, Jan |
| description | Background:
We developed a volumetric single platform image cytometer (SP ICM) that is dedicated to count CD4+ and CD8+ T lymphocytes for HIV monitoring in resource‐constrained settings. The instrument was designed to be low‐cost, yet reliable, easy‐to‐use, and robust.
Methods:
Whole blood is incubated with CD3‐magnetic nanoparticles, CD4‐phycoerythrin (PE), and CD8‐peridinin‐chlorophyll‐protein complex (PerCP). The CD3 cells are immunomagnetically attracted to an analysis surface, where fluorescence images of CD4+ and CD8+ T lymphocytes are recorded and analyzed, respectively. We compared CD4, CD8 counts, and CD4/CD8 ratio obtained by the SP ICM with those from a SP flow cytometer (FCM) tetraCXP method on blood samples from 145 patients.
Results:
Good correlations were obtained (R: 0.96–0.99) between the SP ICM and the SP FCM. There was ∼10% CD8 undercount in the SP ICM, which could be partly caused by CD8+dim T lymphocytes that were not detected by the instrument or not counted by the image analysis due to the cross‐talk from the CD4‐PE signal in the CD8‐PerCP image.
Conclusions:
The SP ICM is a good candidate for HIV monitoring in point‐of‐care settings of resource‐constrained countries. © 2008 Clinical Cytometry Society |
| doi_str_mv | 10.1002/cyto.b.20445 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_66941342</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>66941342</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4025-a3bd8f49669ecd5a35879f27d052af3ff72f38021ef36ce27a2eabdc50a115a53</originalsourceid><addsrcrecordid>eNqF0LtOwzAUxnELgbhvzMgTEy2-xsmIUm4SqEtBYrIc5xgFNTbYiVA3HoFn5ElIaQUbTOcMP33DH6EjSsaUEHZmF10YV2NGhJAbaJdKyUaikGrz5xfFDtpL6ZkQLkWmttEOzXMmlcp20V05Edj4GpeTHIPvW4ima4LHLkR8ffOA2-CbLsTGP-HG4wgp9NHC5_uHDT510TQeapyg6waRDtCWM_MEh-u7j-4vL2bl9eh2enVTnt-OrCBMjgyv6tyJIssKsLU0XOaqcEzVRDLjuHOKOZ4TRsHxzAJThoGpaiuJoVQayffRyWr3JYbXHlKn2yZZmM-Nh9AnPQwLygX7Fw7VFOfZEp6uoI0hpQhOv8SmNXGhKdHLznrZWVf6u_PAj9e7fdVC_YvXYQfAV-CtmcPizzFdPs6mq9kvQS6KgQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20473362</pqid></control><display><type>article</type><title>CD4 and CD8 enumeration for HIV monitoring in resource‐constrained settings</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><source>Wiley Online Library Free Content</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Li, Xiao ; Breukers, Christian ; Ymeti, Aurel ; Lunter, Björn ; Terstappen, Leon W. M. M. ; Greve, Jan</creator><creatorcontrib>Li, Xiao ; Breukers, Christian ; Ymeti, Aurel ; Lunter, Björn ; Terstappen, Leon W. M. M. ; Greve, Jan</creatorcontrib><description>Background:
We developed a volumetric single platform image cytometer (SP ICM) that is dedicated to count CD4+ and CD8+ T lymphocytes for HIV monitoring in resource‐constrained settings. The instrument was designed to be low‐cost, yet reliable, easy‐to‐use, and robust.
Methods:
Whole blood is incubated with CD3‐magnetic nanoparticles, CD4‐phycoerythrin (PE), and CD8‐peridinin‐chlorophyll‐protein complex (PerCP). The CD3 cells are immunomagnetically attracted to an analysis surface, where fluorescence images of CD4+ and CD8+ T lymphocytes are recorded and analyzed, respectively. We compared CD4, CD8 counts, and CD4/CD8 ratio obtained by the SP ICM with those from a SP flow cytometer (FCM) tetraCXP method on blood samples from 145 patients.
Results:
Good correlations were obtained (R: 0.96–0.99) between the SP ICM and the SP FCM. There was ∼10% CD8 undercount in the SP ICM, which could be partly caused by CD8+dim T lymphocytes that were not detected by the instrument or not counted by the image analysis due to the cross‐talk from the CD4‐PE signal in the CD8‐PerCP image.
Conclusions:
The SP ICM is a good candidate for HIV monitoring in point‐of‐care settings of resource‐constrained countries. © 2008 Clinical Cytometry Society</description><identifier>ISSN: 1552-4949</identifier><identifier>EISSN: 1552-4957</identifier><identifier>DOI: 10.1002/cyto.b.20445</identifier><identifier>PMID: 18825776</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>CD4 and CD8 enumeration ; CD4 Antigens - analysis ; CD4 Antigens - metabolism ; CD4 Lymphocyte Count - methods ; CD4-CD8 Ratio - methods ; CD8 Antigens - analysis ; CD8 Antigens - metabolism ; Flow Cytometry - economics ; Flow Cytometry - methods ; Fluorescent Dyes ; HIV Infections - blood ; HIV Infections - diagnosis ; HIV Infections - immunology ; HIV monitoring ; Human immunodeficiency virus ; Humans ; image cytometer ; Image Cytometry - economics ; Image Cytometry - instrumentation ; Image Cytometry - methods ; immunofluorescent labeling ; immunomagnetic separation ; Immunomagnetic Separation - economics ; Immunomagnetic Separation - instrumentation ; Immunomagnetic Separation - methods ; Microscopy, Fluorescence - methods ; Monitoring, Immunologic - economics ; Monitoring, Immunologic - instrumentation ; Monitoring, Immunologic - methods ; point‐of‐care ; Predictive Value of Tests ; single platform ; T-Lymphocytes - immunology ; T-Lymphocytes - virology</subject><ispartof>Cytometry. Part B, Clinical cytometry, 2009-03, Vol.76B (2), p.118-126</ispartof><rights>Copyright © 2008 Clinical Cytometry Society</rights><rights>2008 Clinical Cytometry Society.</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4025-a3bd8f49669ecd5a35879f27d052af3ff72f38021ef36ce27a2eabdc50a115a53</citedby><cites>FETCH-LOGICAL-c4025-a3bd8f49669ecd5a35879f27d052af3ff72f38021ef36ce27a2eabdc50a115a53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fcyto.b.20445$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fcyto.b.20445$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,1427,27901,27902,45550,45551,46384,46808</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18825776$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Xiao</creatorcontrib><creatorcontrib>Breukers, Christian</creatorcontrib><creatorcontrib>Ymeti, Aurel</creatorcontrib><creatorcontrib>Lunter, Björn</creatorcontrib><creatorcontrib>Terstappen, Leon W. M. M.</creatorcontrib><creatorcontrib>Greve, Jan</creatorcontrib><title>CD4 and CD8 enumeration for HIV monitoring in resource‐constrained settings</title><title>Cytometry. Part B, Clinical cytometry</title><addtitle>Cytometry B Clin Cytom</addtitle><description>Background:
We developed a volumetric single platform image cytometer (SP ICM) that is dedicated to count CD4+ and CD8+ T lymphocytes for HIV monitoring in resource‐constrained settings. The instrument was designed to be low‐cost, yet reliable, easy‐to‐use, and robust.
Methods:
Whole blood is incubated with CD3‐magnetic nanoparticles, CD4‐phycoerythrin (PE), and CD8‐peridinin‐chlorophyll‐protein complex (PerCP). The CD3 cells are immunomagnetically attracted to an analysis surface, where fluorescence images of CD4+ and CD8+ T lymphocytes are recorded and analyzed, respectively. We compared CD4, CD8 counts, and CD4/CD8 ratio obtained by the SP ICM with those from a SP flow cytometer (FCM) tetraCXP method on blood samples from 145 patients.
Results:
Good correlations were obtained (R: 0.96–0.99) between the SP ICM and the SP FCM. There was ∼10% CD8 undercount in the SP ICM, which could be partly caused by CD8+dim T lymphocytes that were not detected by the instrument or not counted by the image analysis due to the cross‐talk from the CD4‐PE signal in the CD8‐PerCP image.
Conclusions:
The SP ICM is a good candidate for HIV monitoring in point‐of‐care settings of resource‐constrained countries. © 2008 Clinical Cytometry Society</description><subject>CD4 and CD8 enumeration</subject><subject>CD4 Antigens - analysis</subject><subject>CD4 Antigens - metabolism</subject><subject>CD4 Lymphocyte Count - methods</subject><subject>CD4-CD8 Ratio - methods</subject><subject>CD8 Antigens - analysis</subject><subject>CD8 Antigens - metabolism</subject><subject>Flow Cytometry - economics</subject><subject>Flow Cytometry - methods</subject><subject>Fluorescent Dyes</subject><subject>HIV Infections - blood</subject><subject>HIV Infections - diagnosis</subject><subject>HIV Infections - immunology</subject><subject>HIV monitoring</subject><subject>Human immunodeficiency virus</subject><subject>Humans</subject><subject>image cytometer</subject><subject>Image Cytometry - economics</subject><subject>Image Cytometry - instrumentation</subject><subject>Image Cytometry - methods</subject><subject>immunofluorescent labeling</subject><subject>immunomagnetic separation</subject><subject>Immunomagnetic Separation - economics</subject><subject>Immunomagnetic Separation - instrumentation</subject><subject>Immunomagnetic Separation - methods</subject><subject>Microscopy, Fluorescence - methods</subject><subject>Monitoring, Immunologic - economics</subject><subject>Monitoring, Immunologic - instrumentation</subject><subject>Monitoring, Immunologic - methods</subject><subject>point‐of‐care</subject><subject>Predictive Value of Tests</subject><subject>single platform</subject><subject>T-Lymphocytes - immunology</subject><subject>T-Lymphocytes - virology</subject><issn>1552-4949</issn><issn>1552-4957</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0LtOwzAUxnELgbhvzMgTEy2-xsmIUm4SqEtBYrIc5xgFNTbYiVA3HoFn5ElIaQUbTOcMP33DH6EjSsaUEHZmF10YV2NGhJAbaJdKyUaikGrz5xfFDtpL6ZkQLkWmttEOzXMmlcp20V05Edj4GpeTHIPvW4ima4LHLkR8ffOA2-CbLsTGP-HG4wgp9NHC5_uHDT510TQeapyg6waRDtCWM_MEh-u7j-4vL2bl9eh2enVTnt-OrCBMjgyv6tyJIssKsLU0XOaqcEzVRDLjuHOKOZ4TRsHxzAJThoGpaiuJoVQayffRyWr3JYbXHlKn2yZZmM-Nh9AnPQwLygX7Fw7VFOfZEp6uoI0hpQhOv8SmNXGhKdHLznrZWVf6u_PAj9e7fdVC_YvXYQfAV-CtmcPizzFdPs6mq9kvQS6KgQ</recordid><startdate>200903</startdate><enddate>200903</enddate><creator>Li, Xiao</creator><creator>Breukers, Christian</creator><creator>Ymeti, Aurel</creator><creator>Lunter, Björn</creator><creator>Terstappen, Leon W. M. M.</creator><creator>Greve, Jan</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>200903</creationdate><title>CD4 and CD8 enumeration for HIV monitoring in resource‐constrained settings</title><author>Li, Xiao ; Breukers, Christian ; Ymeti, Aurel ; Lunter, Björn ; Terstappen, Leon W. M. M. ; Greve, Jan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4025-a3bd8f49669ecd5a35879f27d052af3ff72f38021ef36ce27a2eabdc50a115a53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>CD4 and CD8 enumeration</topic><topic>CD4 Antigens - analysis</topic><topic>CD4 Antigens - metabolism</topic><topic>CD4 Lymphocyte Count - methods</topic><topic>CD4-CD8 Ratio - methods</topic><topic>CD8 Antigens - analysis</topic><topic>CD8 Antigens - metabolism</topic><topic>Flow Cytometry - economics</topic><topic>Flow Cytometry - methods</topic><topic>Fluorescent Dyes</topic><topic>HIV Infections - blood</topic><topic>HIV Infections - diagnosis</topic><topic>HIV Infections - immunology</topic><topic>HIV monitoring</topic><topic>Human immunodeficiency virus</topic><topic>Humans</topic><topic>image cytometer</topic><topic>Image Cytometry - economics</topic><topic>Image Cytometry - instrumentation</topic><topic>Image Cytometry - methods</topic><topic>immunofluorescent labeling</topic><topic>immunomagnetic separation</topic><topic>Immunomagnetic Separation - economics</topic><topic>Immunomagnetic Separation - instrumentation</topic><topic>Immunomagnetic Separation - methods</topic><topic>Microscopy, Fluorescence - methods</topic><topic>Monitoring, Immunologic - economics</topic><topic>Monitoring, Immunologic - instrumentation</topic><topic>Monitoring, Immunologic - methods</topic><topic>point‐of‐care</topic><topic>Predictive Value of Tests</topic><topic>single platform</topic><topic>T-Lymphocytes - immunology</topic><topic>T-Lymphocytes - virology</topic><toplevel>online_resources</toplevel><creatorcontrib>Li, Xiao</creatorcontrib><creatorcontrib>Breukers, Christian</creatorcontrib><creatorcontrib>Ymeti, Aurel</creatorcontrib><creatorcontrib>Lunter, Björn</creatorcontrib><creatorcontrib>Terstappen, Leon W. M. M.</creatorcontrib><creatorcontrib>Greve, Jan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cytometry. Part B, Clinical cytometry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Xiao</au><au>Breukers, Christian</au><au>Ymeti, Aurel</au><au>Lunter, Björn</au><au>Terstappen, Leon W. M. M.</au><au>Greve, Jan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CD4 and CD8 enumeration for HIV monitoring in resource‐constrained settings</atitle><jtitle>Cytometry. Part B, Clinical cytometry</jtitle><addtitle>Cytometry B Clin Cytom</addtitle><date>2009-03</date><risdate>2009</risdate><volume>76B</volume><issue>2</issue><spage>118</spage><epage>126</epage><pages>118-126</pages><issn>1552-4949</issn><eissn>1552-4957</eissn><abstract>Background:
We developed a volumetric single platform image cytometer (SP ICM) that is dedicated to count CD4+ and CD8+ T lymphocytes for HIV monitoring in resource‐constrained settings. The instrument was designed to be low‐cost, yet reliable, easy‐to‐use, and robust.
Methods:
Whole blood is incubated with CD3‐magnetic nanoparticles, CD4‐phycoerythrin (PE), and CD8‐peridinin‐chlorophyll‐protein complex (PerCP). The CD3 cells are immunomagnetically attracted to an analysis surface, where fluorescence images of CD4+ and CD8+ T lymphocytes are recorded and analyzed, respectively. We compared CD4, CD8 counts, and CD4/CD8 ratio obtained by the SP ICM with those from a SP flow cytometer (FCM) tetraCXP method on blood samples from 145 patients.
Results:
Good correlations were obtained (R: 0.96–0.99) between the SP ICM and the SP FCM. There was ∼10% CD8 undercount in the SP ICM, which could be partly caused by CD8+dim T lymphocytes that were not detected by the instrument or not counted by the image analysis due to the cross‐talk from the CD4‐PE signal in the CD8‐PerCP image.
Conclusions:
The SP ICM is a good candidate for HIV monitoring in point‐of‐care settings of resource‐constrained countries. © 2008 Clinical Cytometry Society</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>18825776</pmid><doi>10.1002/cyto.b.20445</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1552-4949 |
| ispartof | Cytometry. Part B, Clinical cytometry, 2009-03, Vol.76B (2), p.118-126 |
| issn | 1552-4949 1552-4957 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_66941342 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Wiley Online Library Free Content; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | CD4 and CD8 enumeration CD4 Antigens - analysis CD4 Antigens - metabolism CD4 Lymphocyte Count - methods CD4-CD8 Ratio - methods CD8 Antigens - analysis CD8 Antigens - metabolism Flow Cytometry - economics Flow Cytometry - methods Fluorescent Dyes HIV Infections - blood HIV Infections - diagnosis HIV Infections - immunology HIV monitoring Human immunodeficiency virus Humans image cytometer Image Cytometry - economics Image Cytometry - instrumentation Image Cytometry - methods immunofluorescent labeling immunomagnetic separation Immunomagnetic Separation - economics Immunomagnetic Separation - instrumentation Immunomagnetic Separation - methods Microscopy, Fluorescence - methods Monitoring, Immunologic - economics Monitoring, Immunologic - instrumentation Monitoring, Immunologic - methods point‐of‐care Predictive Value of Tests single platform T-Lymphocytes - immunology T-Lymphocytes - virology |
| title | CD4 and CD8 enumeration for HIV monitoring in resource‐constrained settings |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-08T02%3A19%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=CD4%20and%20CD8%20enumeration%20for%20HIV%20monitoring%20in%20resource%E2%80%90constrained%20settings&rft.jtitle=Cytometry.%20Part%20B,%20Clinical%20cytometry&rft.au=Li,%20Xiao&rft.date=2009-03&rft.volume=76B&rft.issue=2&rft.spage=118&rft.epage=126&rft.pages=118-126&rft.issn=1552-4949&rft.eissn=1552-4957&rft_id=info:doi/10.1002/cyto.b.20445&rft_dat=%3Cproquest_cross%3E66941342%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=20473362&rft_id=info:pmid/18825776&rfr_iscdi=true |