Prostate carcinoma and green tea: PSA‐triggered basement membrane degradation and MMP‐2 activation are inhibited by (−)epigallocatechin‐3‐gallate
Prostate‐specific antigen (PSA) is a serine‐protease that, in addition to cleaving semenogelins in the seminal coagulum, is able to cleave extracellular matrix glycoproteins, thereby affecting cell migration and metastasis. We here report some new activities of PSA that deserve careful consideration...
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| Veröffentlicht in: | International journal of cancer 2004-12, Vol.112 (5), p.787-792 |
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| container_title | International journal of cancer |
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| creator | Pezzato, Elga Sartor, Luigi Dell'Aica, Isabella Dittadi, Ruggero Gion, Massimo Belluco, Claudio Lise, Mario Garbisa, Spiridione |
| description | Prostate‐specific antigen (PSA) is a serine‐protease that, in addition to cleaving semenogelins in the seminal coagulum, is able to cleave extracellular matrix glycoproteins, thereby affecting cell migration and metastasis. We here report some new activities of PSA that deserve careful consideration in the cancer context: degradation of gelatin, degradation of type IV collagen in reconstituted basement membrane (Matrigel) and activation of progelatinase A (MMP‐2), but not pro‐MMP‐9, in a cell‐free system. Since consumption of green tea has been reported to lower the risk of prostate cancer, we investigated the effects of the major flavanol of green tea, (−)epigallocatechin‐3‐gallate (EGCG), on expression and activity of PSA by prostate carcinoma cells. In addition to restraint of PSA expression, EGCG was found to inhibit in a dose‐dependent manner all the above PSA activities, at concentrations lower than the cytotoxic serine‐protease inhibitor PMSF and close to levels measured in the serum following ingestion of green tea. The activity of PSA was suppressed also by the elastase released by the inflammatory leukocytes. These results highlight new PSA activities, suggest gelatin zymography as a new convenient assay for PSA, propose EGCG as natural inhibitor of prostate carcinoma aggressiveness, but also stimulate further investigation on the role of prostatic inflammation. © 2004 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/ijc.20460 |
| format | Article |
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We here report some new activities of PSA that deserve careful consideration in the cancer context: degradation of gelatin, degradation of type IV collagen in reconstituted basement membrane (Matrigel) and activation of progelatinase A (MMP‐2), but not pro‐MMP‐9, in a cell‐free system. Since consumption of green tea has been reported to lower the risk of prostate cancer, we investigated the effects of the major flavanol of green tea, (−)epigallocatechin‐3‐gallate (EGCG), on expression and activity of PSA by prostate carcinoma cells. In addition to restraint of PSA expression, EGCG was found to inhibit in a dose‐dependent manner all the above PSA activities, at concentrations lower than the cytotoxic serine‐protease inhibitor PMSF and close to levels measured in the serum following ingestion of green tea. The activity of PSA was suppressed also by the elastase released by the inflammatory leukocytes. These results highlight new PSA activities, suggest gelatin zymography as a new convenient assay for PSA, propose EGCG as natural inhibitor of prostate carcinoma aggressiveness, but also stimulate further investigation on the role of prostatic inflammation. © 2004 Wiley‐Liss, Inc.</description><identifier>ISSN: 0020-7136</identifier><identifier>EISSN: 1097-0215</identifier><identifier>DOI: 10.1002/ijc.20460</identifier><identifier>PMID: 15386386</identifier><identifier>CODEN: IJCNAW</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>(−)epigallocatechin‐3‐gallate ; Antioxidants - pharmacology ; Basement Membrane - metabolism ; Biological and medical sciences ; Carcinogenesis, carcinogens and anticarcinogens ; Carcinoma - pathology ; Catechin - analogs & derivatives ; Catechin - pharmacology ; Chemical agents ; Gene Expression Regulation, Neoplastic - drug effects ; Humans ; invasion ; Male ; Matrix Metalloproteinase 2 - biosynthesis ; Matrix Metalloproteinase 2 - pharmacology ; Medical sciences ; MMP‐2 activation ; Nephrology. Urinary tract diseases ; prostate carcinoma ; Prostate-Specific Antigen - analysis ; Prostate-Specific Antigen - pharmacology ; prostate‐specific antigen ; Prostatic Neoplasms - pathology ; Risk Factors ; Tea ; Tumor Cells, Cultured ; Tumors ; Tumors of the urinary system ; Urinary tract. 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We here report some new activities of PSA that deserve careful consideration in the cancer context: degradation of gelatin, degradation of type IV collagen in reconstituted basement membrane (Matrigel) and activation of progelatinase A (MMP‐2), but not pro‐MMP‐9, in a cell‐free system. Since consumption of green tea has been reported to lower the risk of prostate cancer, we investigated the effects of the major flavanol of green tea, (−)epigallocatechin‐3‐gallate (EGCG), on expression and activity of PSA by prostate carcinoma cells. In addition to restraint of PSA expression, EGCG was found to inhibit in a dose‐dependent manner all the above PSA activities, at concentrations lower than the cytotoxic serine‐protease inhibitor PMSF and close to levels measured in the serum following ingestion of green tea. The activity of PSA was suppressed also by the elastase released by the inflammatory leukocytes. These results highlight new PSA activities, suggest gelatin zymography as a new convenient assay for PSA, propose EGCG as natural inhibitor of prostate carcinoma aggressiveness, but also stimulate further investigation on the role of prostatic inflammation. © 2004 Wiley‐Liss, Inc.</description><subject>(−)epigallocatechin‐3‐gallate</subject><subject>Antioxidants - pharmacology</subject><subject>Basement Membrane - metabolism</subject><subject>Biological and medical sciences</subject><subject>Carcinogenesis, carcinogens and anticarcinogens</subject><subject>Carcinoma - pathology</subject><subject>Catechin - analogs & derivatives</subject><subject>Catechin - pharmacology</subject><subject>Chemical agents</subject><subject>Gene Expression Regulation, Neoplastic - drug effects</subject><subject>Humans</subject><subject>invasion</subject><subject>Male</subject><subject>Matrix Metalloproteinase 2 - biosynthesis</subject><subject>Matrix Metalloproteinase 2 - pharmacology</subject><subject>Medical sciences</subject><subject>MMP‐2 activation</subject><subject>Nephrology. Urinary tract diseases</subject><subject>prostate carcinoma</subject><subject>Prostate-Specific Antigen - analysis</subject><subject>Prostate-Specific Antigen - pharmacology</subject><subject>prostate‐specific antigen</subject><subject>Prostatic Neoplasms - pathology</subject><subject>Risk Factors</subject><subject>Tea</subject><subject>Tumor Cells, Cultured</subject><subject>Tumors</subject><subject>Tumors of the urinary system</subject><subject>Urinary tract. Prostate gland</subject><issn>0020-7136</issn><issn>1097-0215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9u1DAQxi0EokvhwAsgX0DtIa3HdpxNb9WqlKJWrETvkWNPUlf5s9he0N44ckS99u36JPV2I_WEkGxZ-vybb0bzEfIe2BEwxo_drTniTCr2gsyAlUXGOOQvySz9sawAofbImxBuGQPImXxN9iAXc5XOjNwv_RiijkiN9sYNY6-pHixtPeJAI-oTuvx--vD7b_SubdGjpbUO2OMQaY997fWA1GLrtdXRjcNT8dXVMlVwqk10PyfZI3XDjatd3Fps6MHDn7tDXLlWd91o0gDmxg2pSqS71ZLylrxqdBfw3fTuk-vPZ9eLL9nlt_OLxellZiQULBNNbkFzy-zcisIaKHgNVoIogQsp8lzpUhXQSFvnSqqSyVrUUAghOTeFEvvk08525ccfawyx6l0wmEYYcFyHSqkyWTH4L5gaCwZqnsDDHWjScoPHplp512u_qYBV28SqlFj1lFhiP0ym67pH-0xOESXg4wToYHTXpI0bF545xaUs5yJxxzvul-tw8--O1cXXxa71IysZsSQ</recordid><startdate>20041210</startdate><enddate>20041210</enddate><creator>Pezzato, Elga</creator><creator>Sartor, Luigi</creator><creator>Dell'Aica, Isabella</creator><creator>Dittadi, Ruggero</creator><creator>Gion, Massimo</creator><creator>Belluco, Claudio</creator><creator>Lise, Mario</creator><creator>Garbisa, Spiridione</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U1</scope><scope>7U2</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20041210</creationdate><title>Prostate carcinoma and green tea: PSA‐triggered basement membrane degradation and MMP‐2 activation are inhibited by (−)epigallocatechin‐3‐gallate</title><author>Pezzato, Elga ; Sartor, Luigi ; Dell'Aica, Isabella ; Dittadi, Ruggero ; Gion, Massimo ; Belluco, Claudio ; Lise, Mario ; Garbisa, Spiridione</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4170-3f5d1a2d0d8d37dc172b1d413912343556a9671f4db5646904b3b1733422c763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>(−)epigallocatechin‐3‐gallate</topic><topic>Antioxidants - pharmacology</topic><topic>Basement Membrane - metabolism</topic><topic>Biological and medical sciences</topic><topic>Carcinogenesis, carcinogens and anticarcinogens</topic><topic>Carcinoma - pathology</topic><topic>Catechin - analogs & derivatives</topic><topic>Catechin - pharmacology</topic><topic>Chemical agents</topic><topic>Gene Expression Regulation, Neoplastic - drug effects</topic><topic>Humans</topic><topic>invasion</topic><topic>Male</topic><topic>Matrix Metalloproteinase 2 - biosynthesis</topic><topic>Matrix Metalloproteinase 2 - pharmacology</topic><topic>Medical sciences</topic><topic>MMP‐2 activation</topic><topic>Nephrology. Urinary tract diseases</topic><topic>prostate carcinoma</topic><topic>Prostate-Specific Antigen - analysis</topic><topic>Prostate-Specific Antigen - pharmacology</topic><topic>prostate‐specific antigen</topic><topic>Prostatic Neoplasms - pathology</topic><topic>Risk Factors</topic><topic>Tea</topic><topic>Tumor Cells, Cultured</topic><topic>Tumors</topic><topic>Tumors of the urinary system</topic><topic>Urinary tract. Prostate gland</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pezzato, Elga</creatorcontrib><creatorcontrib>Sartor, Luigi</creatorcontrib><creatorcontrib>Dell'Aica, Isabella</creatorcontrib><creatorcontrib>Dittadi, Ruggero</creatorcontrib><creatorcontrib>Gion, Massimo</creatorcontrib><creatorcontrib>Belluco, Claudio</creatorcontrib><creatorcontrib>Lise, Mario</creatorcontrib><creatorcontrib>Garbisa, Spiridione</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Risk Abstracts</collection><collection>Safety Science and Risk</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pezzato, Elga</au><au>Sartor, Luigi</au><au>Dell'Aica, Isabella</au><au>Dittadi, Ruggero</au><au>Gion, Massimo</au><au>Belluco, Claudio</au><au>Lise, Mario</au><au>Garbisa, Spiridione</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prostate carcinoma and green tea: PSA‐triggered basement membrane degradation and MMP‐2 activation are inhibited by (−)epigallocatechin‐3‐gallate</atitle><jtitle>International journal of cancer</jtitle><addtitle>Int J Cancer</addtitle><date>2004-12-10</date><risdate>2004</risdate><volume>112</volume><issue>5</issue><spage>787</spage><epage>792</epage><pages>787-792</pages><issn>0020-7136</issn><eissn>1097-0215</eissn><coden>IJCNAW</coden><abstract>Prostate‐specific antigen (PSA) is a serine‐protease that, in addition to cleaving semenogelins in the seminal coagulum, is able to cleave extracellular matrix glycoproteins, thereby affecting cell migration and metastasis. We here report some new activities of PSA that deserve careful consideration in the cancer context: degradation of gelatin, degradation of type IV collagen in reconstituted basement membrane (Matrigel) and activation of progelatinase A (MMP‐2), but not pro‐MMP‐9, in a cell‐free system. Since consumption of green tea has been reported to lower the risk of prostate cancer, we investigated the effects of the major flavanol of green tea, (−)epigallocatechin‐3‐gallate (EGCG), on expression and activity of PSA by prostate carcinoma cells. In addition to restraint of PSA expression, EGCG was found to inhibit in a dose‐dependent manner all the above PSA activities, at concentrations lower than the cytotoxic serine‐protease inhibitor PMSF and close to levels measured in the serum following ingestion of green tea. The activity of PSA was suppressed also by the elastase released by the inflammatory leukocytes. These results highlight new PSA activities, suggest gelatin zymography as a new convenient assay for PSA, propose EGCG as natural inhibitor of prostate carcinoma aggressiveness, but also stimulate further investigation on the role of prostatic inflammation. © 2004 Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>15386386</pmid><doi>10.1002/ijc.20460</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | (−)epigallocatechin‐3‐gallate Antioxidants - pharmacology Basement Membrane - metabolism Biological and medical sciences Carcinogenesis, carcinogens and anticarcinogens Carcinoma - pathology Catechin - analogs & derivatives Catechin - pharmacology Chemical agents Gene Expression Regulation, Neoplastic - drug effects Humans invasion Male Matrix Metalloproteinase 2 - biosynthesis Matrix Metalloproteinase 2 - pharmacology Medical sciences MMP‐2 activation Nephrology. Urinary tract diseases prostate carcinoma Prostate-Specific Antigen - analysis Prostate-Specific Antigen - pharmacology prostate‐specific antigen Prostatic Neoplasms - pathology Risk Factors Tea Tumor Cells, Cultured Tumors Tumors of the urinary system Urinary tract. Prostate gland |
| title | Prostate carcinoma and green tea: PSA‐triggered basement membrane degradation and MMP‐2 activation are inhibited by (−)epigallocatechin‐3‐gallate |
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