Mevinolin enhances osteogenic genes (ALP, type I collagen and osteocalcin), CD44, CD47 and CD51 expression during osteogenic differentiation
In this study, we evaluated the effect of mevinolin on the expressions of osteogenic genes and surface molecules expression during osteogenesis. D1 cells were cultured in osteogenic differentiation medium (ODM) for 6 days, treated with mevinolin for 2 days, and then subjected to alizarin red S stain...
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| Veröffentlicht in: | Life sciences (1973) 2009-02, Vol.84 (9), p.290-295 |
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| creator | Kim, Hyung Keun Cho, Sang Gwon Kim, Ji Hyun Doan, Thi Kim Phuong Hu, Qin Sheng Ulhaq, Rehan Song, Eun Kyoo Yoon, Taek Rim |
| description | In this study, we evaluated the effect of mevinolin on the expressions of osteogenic genes and surface molecules expression during osteogenesis.
D1 cells were cultured in osteogenic differentiation medium (ODM) for 6 days, treated with mevinolin for 2 days, and then subjected to alizarin red S staining, MTT assays, alkaline phosphatase (ALP) activity determinations, energy dispersive X-ray spectrophotometry (EDX), real-time PCR, Western blot, fluorescence microscopy and FACS analysis.
Mevinolin is commonly prescribed and widely used to lower cholesterol levels, and offers an important, effective approach to the treatment of hypercholesterolemia and arteriosclerosis. However, the direct effect of mevinolin on osteogenesis
in vitro has not been clarified. ODM has been previously shown to increase the osteoblast differentiation of D1 cells. In the present study, we investigated the expressions of osteogenic genes and surface molecules during osteoblast differentiation induced by mevinolin. We found that the induction of ALP, type I collagen, osteocalcin, CD44, CD47 and CD51 by mevinolin is responsible for the osteoblastic differentiation of D1 cells.
Our data show that mevinolin enhances the expressions of proteins and surface molecules related to osteogenesis. |
| doi_str_mv | 10.1016/j.lfs.2008.12.017 |
| format | Article |
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D1 cells were cultured in osteogenic differentiation medium (ODM) for 6 days, treated with mevinolin for 2 days, and then subjected to alizarin red S staining, MTT assays, alkaline phosphatase (ALP) activity determinations, energy dispersive X-ray spectrophotometry (EDX), real-time PCR, Western blot, fluorescence microscopy and FACS analysis.
Mevinolin is commonly prescribed and widely used to lower cholesterol levels, and offers an important, effective approach to the treatment of hypercholesterolemia and arteriosclerosis. However, the direct effect of mevinolin on osteogenesis
in vitro has not been clarified. ODM has been previously shown to increase the osteoblast differentiation of D1 cells. In the present study, we investigated the expressions of osteogenic genes and surface molecules during osteoblast differentiation induced by mevinolin. We found that the induction of ALP, type I collagen, osteocalcin, CD44, CD47 and CD51 by mevinolin is responsible for the osteoblastic differentiation of D1 cells.
Our data show that mevinolin enhances the expressions of proteins and surface molecules related to osteogenesis.</description><identifier>ISSN: 0024-3205</identifier><identifier>EISSN: 1879-0631</identifier><identifier>DOI: 10.1016/j.lfs.2008.12.017</identifier><identifier>PMID: 19162043</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Alkaline Phosphatase - genetics ; Alkaline Phosphatase - metabolism ; ALP ; Animals ; Bone Marrow Cells - cytology ; CD44 ; CD47 ; CD47 Antigen - genetics ; CD47 Antigen - metabolism ; CD51 ; Cell Differentiation - drug effects ; Cell Differentiation - physiology ; Cell Line ; Collagen Type I - genetics ; Collagen Type I - metabolism ; Gene Expression Regulation - drug effects ; Humans ; Hyaluronan Receptors - genetics ; Hyaluronan Receptors - metabolism ; Hydroxymethylglutaryl-CoA Reductase Inhibitors - pharmacology ; Integrin alphaV - genetics ; Integrin alphaV - metabolism ; Lovastatin - pharmacology ; Mice ; Multipotent Stem Cells - cytology ; Multipotent Stem Cells - physiology ; Osteocalcin ; Osteocalcin - genetics ; Osteocalcin - metabolism ; Osteogenesis - drug effects ; Osteogenesis - genetics ; Statin ; Stromal Cells - cytology ; Type I collagen, Mevinolin</subject><ispartof>Life sciences (1973), 2009-02, Vol.84 (9), p.290-295</ispartof><rights>2008 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c382t-76e63f4662128719666d95c022854daa0812efbd926ad51cb87736693beadb503</citedby><cites>FETCH-LOGICAL-c382t-76e63f4662128719666d95c022854daa0812efbd926ad51cb87736693beadb503</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0024320508005134$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19162043$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Hyung Keun</creatorcontrib><creatorcontrib>Cho, Sang Gwon</creatorcontrib><creatorcontrib>Kim, Ji Hyun</creatorcontrib><creatorcontrib>Doan, Thi Kim Phuong</creatorcontrib><creatorcontrib>Hu, Qin Sheng</creatorcontrib><creatorcontrib>Ulhaq, Rehan</creatorcontrib><creatorcontrib>Song, Eun Kyoo</creatorcontrib><creatorcontrib>Yoon, Taek Rim</creatorcontrib><title>Mevinolin enhances osteogenic genes (ALP, type I collagen and osteocalcin), CD44, CD47 and CD51 expression during osteogenic differentiation</title><title>Life sciences (1973)</title><addtitle>Life Sci</addtitle><description>In this study, we evaluated the effect of mevinolin on the expressions of osteogenic genes and surface molecules expression during osteogenesis.
D1 cells were cultured in osteogenic differentiation medium (ODM) for 6 days, treated with mevinolin for 2 days, and then subjected to alizarin red S staining, MTT assays, alkaline phosphatase (ALP) activity determinations, energy dispersive X-ray spectrophotometry (EDX), real-time PCR, Western blot, fluorescence microscopy and FACS analysis.
Mevinolin is commonly prescribed and widely used to lower cholesterol levels, and offers an important, effective approach to the treatment of hypercholesterolemia and arteriosclerosis. However, the direct effect of mevinolin on osteogenesis
in vitro has not been clarified. ODM has been previously shown to increase the osteoblast differentiation of D1 cells. In the present study, we investigated the expressions of osteogenic genes and surface molecules during osteoblast differentiation induced by mevinolin. We found that the induction of ALP, type I collagen, osteocalcin, CD44, CD47 and CD51 by mevinolin is responsible for the osteoblastic differentiation of D1 cells.
Our data show that mevinolin enhances the expressions of proteins and surface molecules related to osteogenesis.</description><subject>Alkaline Phosphatase - genetics</subject><subject>Alkaline Phosphatase - metabolism</subject><subject>ALP</subject><subject>Animals</subject><subject>Bone Marrow Cells - cytology</subject><subject>CD44</subject><subject>CD47</subject><subject>CD47 Antigen - genetics</subject><subject>CD47 Antigen - metabolism</subject><subject>CD51</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Differentiation - physiology</subject><subject>Cell Line</subject><subject>Collagen Type I - genetics</subject><subject>Collagen Type I - metabolism</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Humans</subject><subject>Hyaluronan Receptors - genetics</subject><subject>Hyaluronan Receptors - metabolism</subject><subject>Hydroxymethylglutaryl-CoA Reductase Inhibitors - pharmacology</subject><subject>Integrin alphaV - genetics</subject><subject>Integrin alphaV - metabolism</subject><subject>Lovastatin - pharmacology</subject><subject>Mice</subject><subject>Multipotent Stem Cells - cytology</subject><subject>Multipotent Stem Cells - physiology</subject><subject>Osteocalcin</subject><subject>Osteocalcin - genetics</subject><subject>Osteocalcin - metabolism</subject><subject>Osteogenesis - drug effects</subject><subject>Osteogenesis - genetics</subject><subject>Statin</subject><subject>Stromal Cells - cytology</subject><subject>Type I collagen, Mevinolin</subject><issn>0024-3205</issn><issn>1879-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhi0EokvhAbggnxBITfDYiZ2IU7UFWmkRHOBsOfakeJW1Fztb0XfgofF2V6Kn9jKWxt_80sxHyGtgNTCQH9b1NOaaM9bVwGsG6glZQKf6ikkBT8mCMd5UgrP2hLzIec0Ya1slnpMT6EFy1ogF-fsVb3yIkw8Uwy8TLGYa84zxGoO3tNTSeHe--n5G59st0itq4zSZ0qcmuANqzWR9eH9GlxdNc1fV3efyogWKf7YJc_YxULdLPlzfj3d-HDFhmL2ZC_GSPBvNlPHV8T0lPz9_-rG8rFbfvlwtz1eVFR2fKyVRirGRkgPvFPRSSte3lnHetY0zhnXAcRxcz6VxLdihU0pI2YsBjRtaJk7J20PuNsXfO8yz3vhssewVMO6y3rMgpHoULKcVouv6AsIBtCnmnHDU2-Q3Jt1qYHrvSq91caX3rjRwXVyVmTfH8N2wQfd_4iinAB8PAJZb3HhMOluPRZHzCe2sXfQPxP8DKCqjTg</recordid><startdate>20090227</startdate><enddate>20090227</enddate><creator>Kim, Hyung Keun</creator><creator>Cho, Sang Gwon</creator><creator>Kim, Ji Hyun</creator><creator>Doan, Thi Kim Phuong</creator><creator>Hu, Qin Sheng</creator><creator>Ulhaq, Rehan</creator><creator>Song, Eun Kyoo</creator><creator>Yoon, Taek Rim</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20090227</creationdate><title>Mevinolin enhances osteogenic genes (ALP, type I collagen and osteocalcin), CD44, CD47 and CD51 expression during osteogenic differentiation</title><author>Kim, Hyung Keun ; Cho, Sang Gwon ; Kim, Ji Hyun ; Doan, Thi Kim Phuong ; Hu, Qin Sheng ; Ulhaq, Rehan ; Song, Eun Kyoo ; Yoon, Taek Rim</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c382t-76e63f4662128719666d95c022854daa0812efbd926ad51cb87736693beadb503</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Alkaline Phosphatase - genetics</topic><topic>Alkaline Phosphatase - metabolism</topic><topic>ALP</topic><topic>Animals</topic><topic>Bone Marrow Cells - cytology</topic><topic>CD44</topic><topic>CD47</topic><topic>CD47 Antigen - genetics</topic><topic>CD47 Antigen - metabolism</topic><topic>CD51</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Differentiation - physiology</topic><topic>Cell Line</topic><topic>Collagen Type I - genetics</topic><topic>Collagen Type I - metabolism</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Humans</topic><topic>Hyaluronan Receptors - genetics</topic><topic>Hyaluronan Receptors - metabolism</topic><topic>Hydroxymethylglutaryl-CoA Reductase Inhibitors - pharmacology</topic><topic>Integrin alphaV - genetics</topic><topic>Integrin alphaV - metabolism</topic><topic>Lovastatin - pharmacology</topic><topic>Mice</topic><topic>Multipotent Stem Cells - cytology</topic><topic>Multipotent Stem Cells - physiology</topic><topic>Osteocalcin</topic><topic>Osteocalcin - genetics</topic><topic>Osteocalcin - metabolism</topic><topic>Osteogenesis - drug effects</topic><topic>Osteogenesis - genetics</topic><topic>Statin</topic><topic>Stromal Cells - cytology</topic><topic>Type I collagen, Mevinolin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Hyung Keun</creatorcontrib><creatorcontrib>Cho, Sang Gwon</creatorcontrib><creatorcontrib>Kim, Ji Hyun</creatorcontrib><creatorcontrib>Doan, Thi Kim Phuong</creatorcontrib><creatorcontrib>Hu, Qin Sheng</creatorcontrib><creatorcontrib>Ulhaq, Rehan</creatorcontrib><creatorcontrib>Song, Eun Kyoo</creatorcontrib><creatorcontrib>Yoon, Taek Rim</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Life sciences (1973)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Hyung Keun</au><au>Cho, Sang Gwon</au><au>Kim, Ji Hyun</au><au>Doan, Thi Kim Phuong</au><au>Hu, Qin Sheng</au><au>Ulhaq, Rehan</au><au>Song, Eun Kyoo</au><au>Yoon, Taek Rim</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mevinolin enhances osteogenic genes (ALP, type I collagen and osteocalcin), CD44, CD47 and CD51 expression during osteogenic differentiation</atitle><jtitle>Life sciences (1973)</jtitle><addtitle>Life Sci</addtitle><date>2009-02-27</date><risdate>2009</risdate><volume>84</volume><issue>9</issue><spage>290</spage><epage>295</epage><pages>290-295</pages><issn>0024-3205</issn><eissn>1879-0631</eissn><abstract>In this study, we evaluated the effect of mevinolin on the expressions of osteogenic genes and surface molecules expression during osteogenesis.
D1 cells were cultured in osteogenic differentiation medium (ODM) for 6 days, treated with mevinolin for 2 days, and then subjected to alizarin red S staining, MTT assays, alkaline phosphatase (ALP) activity determinations, energy dispersive X-ray spectrophotometry (EDX), real-time PCR, Western blot, fluorescence microscopy and FACS analysis.
Mevinolin is commonly prescribed and widely used to lower cholesterol levels, and offers an important, effective approach to the treatment of hypercholesterolemia and arteriosclerosis. However, the direct effect of mevinolin on osteogenesis
in vitro has not been clarified. ODM has been previously shown to increase the osteoblast differentiation of D1 cells. In the present study, we investigated the expressions of osteogenic genes and surface molecules during osteoblast differentiation induced by mevinolin. We found that the induction of ALP, type I collagen, osteocalcin, CD44, CD47 and CD51 by mevinolin is responsible for the osteoblastic differentiation of D1 cells.
Our data show that mevinolin enhances the expressions of proteins and surface molecules related to osteogenesis.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>19162043</pmid><doi>10.1016/j.lfs.2008.12.017</doi><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 0024-3205 |
| ispartof | Life sciences (1973), 2009-02, Vol.84 (9), p.290-295 |
| issn | 0024-3205 1879-0631 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Alkaline Phosphatase - genetics Alkaline Phosphatase - metabolism ALP Animals Bone Marrow Cells - cytology CD44 CD47 CD47 Antigen - genetics CD47 Antigen - metabolism CD51 Cell Differentiation - drug effects Cell Differentiation - physiology Cell Line Collagen Type I - genetics Collagen Type I - metabolism Gene Expression Regulation - drug effects Humans Hyaluronan Receptors - genetics Hyaluronan Receptors - metabolism Hydroxymethylglutaryl-CoA Reductase Inhibitors - pharmacology Integrin alphaV - genetics Integrin alphaV - metabolism Lovastatin - pharmacology Mice Multipotent Stem Cells - cytology Multipotent Stem Cells - physiology Osteocalcin Osteocalcin - genetics Osteocalcin - metabolism Osteogenesis - drug effects Osteogenesis - genetics Statin Stromal Cells - cytology Type I collagen, Mevinolin |
| title | Mevinolin enhances osteogenic genes (ALP, type I collagen and osteocalcin), CD44, CD47 and CD51 expression during osteogenic differentiation |
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