Detection of stanozolol and its metabolites in equine urine by liquid chromatography-electrospray ionization ion trap mass spectrometry
The equine phase I and phase II metabolism of the synthetic anabolic steroid stanozolol was investigated following its administration by intramuscular injection to a thoroughbred gelding. The major phase I biotransformations were hydroxylation at C16 and one other site, while phase II metabolism in...
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| Veröffentlicht in: | Journal of chromatography. B 2004-11, Vol.811 (1), p.75-83 |
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| container_end_page | 83 |
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| container_title | Journal of chromatography. B |
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| creator | MCKINNEY, Andrew R SUANN, Craig J DUNSTAN, Anthony J MULLEY, Stephen L RIDLEY, Damon D STENHOUSE, Allen M |
| description | The equine phase I and phase II metabolism of the synthetic anabolic steroid stanozolol was investigated following its administration by intramuscular injection to a thoroughbred gelding. The major phase I biotransformations were hydroxylation at C16 and one other site, while phase II metabolism in the form of sulfate and beta-glucuronide conjugation was extensive. An analytical procedure was developed for the detection of stanozolol and its metabolites in equine urine using solid phase extraction, acid solvolysis of phase II conjugates and analysis by positive ion electrospray ionization ion trap LC-MS. |
| doi_str_mv | 10.1016/s1570-0232(04)00634-8 |
| format | Article |
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An analytical procedure was developed for the detection of stanozolol and its metabolites in equine urine using solid phase extraction, acid solvolysis of phase II conjugates and analysis by positive ion electrospray ionization ion trap LC-MS.</description><subject>Anabolic Agents - pharmacokinetics</subject><subject>Anabolic Agents - urine</subject><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biotransformation</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>Horses</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Pharmacology. 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| ispartof | Journal of chromatography. B, 2004-11, Vol.811 (1), p.75-83 |
| issn | 1570-0232 1873-376X |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Anabolic Agents - pharmacokinetics Anabolic Agents - urine Analysis Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Biotransformation Enzyme-Linked Immunosorbent Assay Fundamental and applied biological sciences. Psychology General pharmacology Horses Male Medical sciences Pharmacology. Drug treatments Spectrometry, Mass, Electrospray Ionization - methods Stanozolol - pharmacokinetics Stanozolol - urine |
| title | Detection of stanozolol and its metabolites in equine urine by liquid chromatography-electrospray ionization ion trap mass spectrometry |
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