Rapid Identification of the Candida Species from Direct Blood Cultures by CHROMagar™ Candida
We evaluated the ability of CHROMagar™ Candida to identify Candida species isolated directly from blood cultures. A total of 50 clinical isolates of Candida were incubated at 35°C, and once growth was established, an aliquot of each was plated onto CHROMagar™ Candida medium. A control specimen was p...
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| Veröffentlicht in: | Journal of international medical research 2004-09, Vol.32 (5), p.484-487 |
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| creator | Birinci, A Akkurt, L Acuner, C Unlu, M Durupinar, B |
| description | We evaluated the ability of CHROMagar™ Candida to identify Candida species isolated directly from blood cultures. A total of 50 clinical isolates of Candida were incubated at 35°C, and once growth was established, an aliquot of each was plated onto CHROMagar™ Candida medium. A control specimen was plated directly from Sabouraud's dextrose agar. Following incubation at 30°C, all yeast isolates were identified by colony morphology and colour. We were able to identify all isolates of C. albicans (n = 20), C. tropicalis (n = 14), C. glabrata (n = 6), and C. krusei (n = 5), which were isolated from blood or from control cultures. This study demonstrated that CHROMagar™ Candida reliably isolated and identified yeast taken directly from blood cultures. We conclude that this rapid and easy method of identifying Candida species will enable clinicians to quickly choose the appropriate antifungal agent. This should decrease patient morbidity and mortality. |
| doi_str_mv | 10.1177/147323000403200504 |
| format | Article |
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A total of 50 clinical isolates of Candida were incubated at 35°C, and once growth was established, an aliquot of each was plated onto CHROMagar™ Candida medium. A control specimen was plated directly from Sabouraud's dextrose agar. Following incubation at 30°C, all yeast isolates were identified by colony morphology and colour. We were able to identify all isolates of C. albicans (n = 20), C. tropicalis (n = 14), C. glabrata (n = 6), and C. krusei (n = 5), which were isolated from blood or from control cultures. This study demonstrated that CHROMagar™ Candida reliably isolated and identified yeast taken directly from blood cultures. We conclude that this rapid and easy method of identifying Candida species will enable clinicians to quickly choose the appropriate antifungal agent. This should decrease patient morbidity and mortality.</description><identifier>ISSN: 0300-0605</identifier><identifier>EISSN: 1473-2300</identifier><identifier>DOI: 10.1177/147323000403200504</identifier><identifier>PMID: 15458279</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Blood - microbiology ; Candida - classification ; Candida - isolation & purification ; Candidiasis - diagnosis ; Cell Culture Techniques - methods ; Chromogenic Compounds ; Humans ; Mycological Typing Techniques</subject><ispartof>Journal of international medical research, 2004-09, Vol.32 (5), p.484-487</ispartof><rights>2004 SAGE Publications</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c339t-f430910bfee53459a0341c3dd4d82839d0d81788c0f54e3413ff391ebaf9c7533</citedby><cites>FETCH-LOGICAL-c339t-f430910bfee53459a0341c3dd4d82839d0d81788c0f54e3413ff391ebaf9c7533</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/147323000403200504$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/147323000403200504$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,780,784,21966,27853,27924,27925,44945,45333</link.rule.ids><linktorsrc>$$Uhttps://journals.sagepub.com/doi/full/10.1177/147323000403200504?utm_source=summon&utm_medium=discovery-provider$$EView_record_in_SAGE_Publications$$FView_record_in_$$GSAGE_Publications</linktorsrc><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15458279$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Birinci, A</creatorcontrib><creatorcontrib>Akkurt, L</creatorcontrib><creatorcontrib>Acuner, C</creatorcontrib><creatorcontrib>Unlu, M</creatorcontrib><creatorcontrib>Durupinar, B</creatorcontrib><title>Rapid Identification of the Candida Species from Direct Blood Cultures by CHROMagar™ Candida</title><title>Journal of international medical research</title><addtitle>J Int Med Res</addtitle><description>We evaluated the ability of CHROMagar™ Candida to identify Candida species isolated directly from blood cultures. A total of 50 clinical isolates of Candida were incubated at 35°C, and once growth was established, an aliquot of each was plated onto CHROMagar™ Candida medium. A control specimen was plated directly from Sabouraud's dextrose agar. Following incubation at 30°C, all yeast isolates were identified by colony morphology and colour. We were able to identify all isolates of C. albicans (n = 20), C. tropicalis (n = 14), C. glabrata (n = 6), and C. krusei (n = 5), which were isolated from blood or from control cultures. This study demonstrated that CHROMagar™ Candida reliably isolated and identified yeast taken directly from blood cultures. We conclude that this rapid and easy method of identifying Candida species will enable clinicians to quickly choose the appropriate antifungal agent. This should decrease patient morbidity and mortality.</description><subject>Blood - microbiology</subject><subject>Candida - classification</subject><subject>Candida - isolation & purification</subject><subject>Candidiasis - diagnosis</subject><subject>Cell Culture Techniques - methods</subject><subject>Chromogenic Compounds</subject><subject>Humans</subject><subject>Mycological Typing Techniques</subject><issn>0300-0605</issn><issn>1473-2300</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMlKxEAYhBtRnHF5AQ_SJ2_Rv7csR40rKILL1dDpZWxJ0mN3cpi7T-Kj-SRmmBEPgqcfqr4q-AuhAwLHhGTZCeEZowwAODAKIIBvoOlSTJbqJprCeBJIQUzQToxvI0hTQbfRhAgucpoVU_TyIOdO4xttut5Zp2TvfIe9xf2rwaXstNMSP86NciZiG3yLz10wqsdnjfcal0PTD2G06gUurx_u7-RMhq-Pz5_oHtqysolmf3130fPlxVN5ndzeX92Up7eJYqzoE8sZFARqa4xgXBQSGCeKac11TnNWaNA5yfJcgRXcjB6zlhXE1NIWKhOM7aKjVe88-PfBxL5qXVSmaWRn_BCrNC0YUEJHkK5AFXyMwdhqHlwrw6IiUC1Xrf6uOoYO1-1D3Rr9G1nPOAInKyDKmane_BC68dv_Kr8BZyl-pA</recordid><startdate>20040901</startdate><enddate>20040901</enddate><creator>Birinci, A</creator><creator>Akkurt, L</creator><creator>Acuner, C</creator><creator>Unlu, M</creator><creator>Durupinar, B</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20040901</creationdate><title>Rapid Identification of the Candida Species from Direct Blood Cultures by CHROMagar™ Candida</title><author>Birinci, A ; Akkurt, L ; Acuner, C ; Unlu, M ; Durupinar, B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c339t-f430910bfee53459a0341c3dd4d82839d0d81788c0f54e3413ff391ebaf9c7533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Blood - microbiology</topic><topic>Candida - classification</topic><topic>Candida - isolation & purification</topic><topic>Candidiasis - diagnosis</topic><topic>Cell Culture Techniques - methods</topic><topic>Chromogenic Compounds</topic><topic>Humans</topic><topic>Mycological Typing Techniques</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Birinci, A</creatorcontrib><creatorcontrib>Akkurt, L</creatorcontrib><creatorcontrib>Acuner, C</creatorcontrib><creatorcontrib>Unlu, M</creatorcontrib><creatorcontrib>Durupinar, B</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of international medical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Birinci, A</au><au>Akkurt, L</au><au>Acuner, C</au><au>Unlu, M</au><au>Durupinar, B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid Identification of the Candida Species from Direct Blood Cultures by CHROMagar™ Candida</atitle><jtitle>Journal of international medical research</jtitle><addtitle>J Int Med Res</addtitle><date>2004-09-01</date><risdate>2004</risdate><volume>32</volume><issue>5</issue><spage>484</spage><epage>487</epage><pages>484-487</pages><issn>0300-0605</issn><eissn>1473-2300</eissn><abstract>We evaluated the ability of CHROMagar™ Candida to identify Candida species isolated directly from blood cultures. A total of 50 clinical isolates of Candida were incubated at 35°C, and once growth was established, an aliquot of each was plated onto CHROMagar™ Candida medium. A control specimen was plated directly from Sabouraud's dextrose agar. Following incubation at 30°C, all yeast isolates were identified by colony morphology and colour. We were able to identify all isolates of C. albicans (n = 20), C. tropicalis (n = 14), C. glabrata (n = 6), and C. krusei (n = 5), which were isolated from blood or from control cultures. This study demonstrated that CHROMagar™ Candida reliably isolated and identified yeast taken directly from blood cultures. We conclude that this rapid and easy method of identifying Candida species will enable clinicians to quickly choose the appropriate antifungal agent. 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| ispartof | Journal of international medical research, 2004-09, Vol.32 (5), p.484-487 |
| issn | 0300-0605 1473-2300 |
| language | eng |
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| source | Sage Journals GOLD Open Access 2024 |
| subjects | Blood - microbiology Candida - classification Candida - isolation & purification Candidiasis - diagnosis Cell Culture Techniques - methods Chromogenic Compounds Humans Mycological Typing Techniques |
| title | Rapid Identification of the Candida Species from Direct Blood Cultures by CHROMagar™ Candida |
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