Altered pattern of cannabinoid type 1 receptor expression in adipose tissue of dysmetabolic and overweight patients
Abstract In overweight patients (OW), the increased peripheral activity of the endocannabinoid system in visceral adipose tissue (VAT) may be mediated by cannabinoid type 1 (CB1) receptor expression. We determined whether CB1 receptor splice variants and messenger RNA (mRNA) levels in perirenal and...
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| creator | Sarzani, Riccardo Bordicchia, Marica Marcucci, Pierfrancesco Bedetta, Samuele Santini, Silvia Giovagnoli, Andrea Scappini, Lorena Minardi, Daniele Muzzonigro, Giovanni Dessì-Fulgheri, Paolo Rappelli, Alessandro |
| description | Abstract In overweight patients (OW), the increased peripheral activity of the endocannabinoid system in visceral adipose tissue (VAT) may be mediated by cannabinoid type 1 (CB1) receptor expression. We determined whether CB1 receptor splice variants and messenger RNA (mRNA) levels in perirenal and subcutaneous adipose tissues are associated with obesity and metabolic syndrome (MetS). Gene expression with multiple-primers real-time polymerase chain reaction (TaqMan; Applied Biosystem, Weiterstadt, Germany) was performed to study VAT and paired subcutaneous adipose tissue (SAT) mRNA from 36 consecutive patients undergoing nephrectomy. Cannabinoid type 1A and CB1E mRNAs variants with the longer version of exon 4 were expressed. The CB1 expression in perirenal VAT significantly correlated with body mass index (BMI). Paired subcutaneous/perirenal samples from normal-weight patients (BMI |
| doi_str_mv | 10.1016/j.metabol.2008.10.009 |
| format | Article |
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We determined whether CB1 receptor splice variants and messenger RNA (mRNA) levels in perirenal and subcutaneous adipose tissues are associated with obesity and metabolic syndrome (MetS). Gene expression with multiple-primers real-time polymerase chain reaction (TaqMan; Applied Biosystem, Weiterstadt, Germany) was performed to study VAT and paired subcutaneous adipose tissue (SAT) mRNA from 36 consecutive patients undergoing nephrectomy. Cannabinoid type 1A and CB1E mRNAs variants with the longer version of exon 4 were expressed. The CB1 expression in perirenal VAT significantly correlated with body mass index (BMI). Paired subcutaneous/perirenal samples from normal-weight patients (BMI <25 kg/m2 ) showed higher CB1 expression in SAT ( P = .002), whereas in OW (BMI ≥25 kg/m2 ), the higher CB1 expression was in VAT ( P = .038). In unpaired samples, SAT of normal-weight patients had significantly higher CB1 mRNA levels compared with SAT of OW, whereas higher CB1 expression ( P = .009) was found in VAT of OW (n = 25). Overweight patients with increased visceral CB1 expression had higher waist circumference ( P < .01), insulin ( P < .01), and homeostasis model assessment index ( P < .01). In addition, patients with the MetS (n = 22) showed higher CB1 expression in perirenal adipose tissues ( P = .007). Visceral adipose CB1 expression correlated with BMI. Overweight patients and those with MetS showed a CB1 expression pattern supporting a CB1-mediated overactivity of the endocannabinoid system in human VAT.</description><identifier>ISSN: 0026-0495</identifier><identifier>EISSN: 1532-8600</identifier><identifier>DOI: 10.1016/j.metabol.2008.10.009</identifier><identifier>PMID: 19217452</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Adipose Tissue - physiopathology ; Alternative Splicing ; Biological and medical sciences ; Body Mass Index ; Body Weight - genetics ; DNA, Single-Stranded - genetics ; Endocrinology & Metabolism ; Exons ; Feeding. Feeding behavior ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation ; Genetic Variation ; Humans ; Kidney Neoplasms - genetics ; Kidney Neoplasms - pathology ; Medical sciences ; Metabolic diseases ; Metabolic Diseases - genetics ; Metabolic Diseases - physiopathology ; Metabolic Syndrome - genetics ; Obesity ; Obesity - genetics ; Overweight - genetics ; Overweight - physiopathology ; Receptor, Cannabinoid, CB1 - genetics ; RNA, Messenger - genetics ; Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><ispartof>Metabolism, clinical and experimental, 2009-03, Vol.58 (3), p.361-367</ispartof><rights>Elsevier Inc.</rights><rights>2009 Elsevier Inc.</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c448t-f8191325c7e07907a42a01134859510a7e2b14d133d2a5e7ad40ec4aca05406d3</citedby><cites>FETCH-LOGICAL-c448t-f8191325c7e07907a42a01134859510a7e2b14d133d2a5e7ad40ec4aca05406d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.metabol.2008.10.009$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21200144$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19217452$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sarzani, Riccardo</creatorcontrib><creatorcontrib>Bordicchia, Marica</creatorcontrib><creatorcontrib>Marcucci, Pierfrancesco</creatorcontrib><creatorcontrib>Bedetta, Samuele</creatorcontrib><creatorcontrib>Santini, Silvia</creatorcontrib><creatorcontrib>Giovagnoli, Andrea</creatorcontrib><creatorcontrib>Scappini, Lorena</creatorcontrib><creatorcontrib>Minardi, Daniele</creatorcontrib><creatorcontrib>Muzzonigro, Giovanni</creatorcontrib><creatorcontrib>Dessì-Fulgheri, Paolo</creatorcontrib><creatorcontrib>Rappelli, Alessandro</creatorcontrib><title>Altered pattern of cannabinoid type 1 receptor expression in adipose tissue of dysmetabolic and overweight patients</title><title>Metabolism, clinical and experimental</title><addtitle>Metabolism</addtitle><description>Abstract In overweight patients (OW), the increased peripheral activity of the endocannabinoid system in visceral adipose tissue (VAT) may be mediated by cannabinoid type 1 (CB1) receptor expression. We determined whether CB1 receptor splice variants and messenger RNA (mRNA) levels in perirenal and subcutaneous adipose tissues are associated with obesity and metabolic syndrome (MetS). Gene expression with multiple-primers real-time polymerase chain reaction (TaqMan; Applied Biosystem, Weiterstadt, Germany) was performed to study VAT and paired subcutaneous adipose tissue (SAT) mRNA from 36 consecutive patients undergoing nephrectomy. Cannabinoid type 1A and CB1E mRNAs variants with the longer version of exon 4 were expressed. The CB1 expression in perirenal VAT significantly correlated with body mass index (BMI). Paired subcutaneous/perirenal samples from normal-weight patients (BMI <25 kg/m2 ) showed higher CB1 expression in SAT ( P = .002), whereas in OW (BMI ≥25 kg/m2 ), the higher CB1 expression was in VAT ( P = .038). In unpaired samples, SAT of normal-weight patients had significantly higher CB1 mRNA levels compared with SAT of OW, whereas higher CB1 expression ( P = .009) was found in VAT of OW (n = 25). Overweight patients with increased visceral CB1 expression had higher waist circumference ( P < .01), insulin ( P < .01), and homeostasis model assessment index ( P < .01). In addition, patients with the MetS (n = 22) showed higher CB1 expression in perirenal adipose tissues ( P = .007). Visceral adipose CB1 expression correlated with BMI. Overweight patients and those with MetS showed a CB1 expression pattern supporting a CB1-mediated overactivity of the endocannabinoid system in human VAT.</description><subject>Adipose Tissue - physiopathology</subject><subject>Alternative Splicing</subject><subject>Biological and medical sciences</subject><subject>Body Mass Index</subject><subject>Body Weight - genetics</subject><subject>DNA, Single-Stranded - genetics</subject><subject>Endocrinology & Metabolism</subject><subject>Exons</subject><subject>Feeding. Feeding behavior</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation</subject><subject>Genetic Variation</subject><subject>Humans</subject><subject>Kidney Neoplasms - genetics</subject><subject>Kidney Neoplasms - pathology</subject><subject>Medical sciences</subject><subject>Metabolic diseases</subject><subject>Metabolic Diseases - genetics</subject><subject>Metabolic Diseases - physiopathology</subject><subject>Metabolic Syndrome - genetics</subject><subject>Obesity</subject><subject>Obesity - genetics</subject><subject>Overweight - genetics</subject><subject>Overweight - physiopathology</subject><subject>Receptor, Cannabinoid, CB1 - genetics</subject><subject>RNA, Messenger - genetics</subject><subject>Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><issn>0026-0495</issn><issn>1532-8600</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkk2P0zAQQCMEYrsLPwHkC9xSxo6TOBfQasUC0kocgLPl2lNwSe3gcRb673HUCCQunGyN3nw9TVU947DlwLtXh-0Rs9nFcSsAVIltAYYH1Ya3jahVB_Cw2gCIrgY5tBfVJdEBAPpedY-rCz4I3stWbCq6HjMmdGwyuXwCi3tmTQhm50P0juXThIyzhBanHBPDX1NCIh8D84EZ56dIyLInmnHJdSda5_KWmeBYvMf0E_3Xb3lp4TFkelI92puR8On6XlVfbt9-vnlf33189-Hm-q62Uqpc7xUfeCNa2yP0A_RGCgOcN1K1Q8vB9Ch2XDreNE6YFnvjJKCVxhpoJXSuuapenutOKf6YkbI-erI4jiZgnEl33SBUx1UB2zNoUyRKuNdT8keTTpqDXmzrg1630ovtJVxsl7zna4N5d0T3N2vVW4AXK2DImnGfTLCe_nCCl2pcysK9OXNYdNx7TJpsUWXR-WI-axf9f0d5_U8FO_rgS9PveEI6xDmF4lpzTUKD_rScxnIZoACaQTXNb6a-tjI</recordid><startdate>20090301</startdate><enddate>20090301</enddate><creator>Sarzani, Riccardo</creator><creator>Bordicchia, Marica</creator><creator>Marcucci, Pierfrancesco</creator><creator>Bedetta, Samuele</creator><creator>Santini, Silvia</creator><creator>Giovagnoli, Andrea</creator><creator>Scappini, Lorena</creator><creator>Minardi, Daniele</creator><creator>Muzzonigro, Giovanni</creator><creator>Dessì-Fulgheri, Paolo</creator><creator>Rappelli, Alessandro</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20090301</creationdate><title>Altered pattern of cannabinoid type 1 receptor expression in adipose tissue of dysmetabolic and overweight patients</title><author>Sarzani, Riccardo ; Bordicchia, Marica ; Marcucci, Pierfrancesco ; Bedetta, Samuele ; Santini, Silvia ; Giovagnoli, Andrea ; Scappini, Lorena ; Minardi, Daniele ; Muzzonigro, Giovanni ; Dessì-Fulgheri, Paolo ; Rappelli, Alessandro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c448t-f8191325c7e07907a42a01134859510a7e2b14d133d2a5e7ad40ec4aca05406d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Adipose Tissue - physiopathology</topic><topic>Alternative Splicing</topic><topic>Biological and medical sciences</topic><topic>Body Mass Index</topic><topic>Body Weight - genetics</topic><topic>DNA, Single-Stranded - genetics</topic><topic>Endocrinology & Metabolism</topic><topic>Exons</topic><topic>Feeding. 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Psychology</topic><topic>Gene Expression Regulation</topic><topic>Genetic Variation</topic><topic>Humans</topic><topic>Kidney Neoplasms - genetics</topic><topic>Kidney Neoplasms - pathology</topic><topic>Medical sciences</topic><topic>Metabolic diseases</topic><topic>Metabolic Diseases - genetics</topic><topic>Metabolic Diseases - physiopathology</topic><topic>Metabolic Syndrome - genetics</topic><topic>Obesity</topic><topic>Obesity - genetics</topic><topic>Overweight - genetics</topic><topic>Overweight - physiopathology</topic><topic>Receptor, Cannabinoid, CB1 - genetics</topic><topic>RNA, Messenger - genetics</topic><topic>Vertebrates: anatomy and physiology, studies on body, several organs or systems</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sarzani, Riccardo</creatorcontrib><creatorcontrib>Bordicchia, Marica</creatorcontrib><creatorcontrib>Marcucci, Pierfrancesco</creatorcontrib><creatorcontrib>Bedetta, Samuele</creatorcontrib><creatorcontrib>Santini, Silvia</creatorcontrib><creatorcontrib>Giovagnoli, Andrea</creatorcontrib><creatorcontrib>Scappini, Lorena</creatorcontrib><creatorcontrib>Minardi, Daniele</creatorcontrib><creatorcontrib>Muzzonigro, Giovanni</creatorcontrib><creatorcontrib>Dessì-Fulgheri, Paolo</creatorcontrib><creatorcontrib>Rappelli, Alessandro</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Metabolism, clinical and experimental</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sarzani, Riccardo</au><au>Bordicchia, Marica</au><au>Marcucci, Pierfrancesco</au><au>Bedetta, Samuele</au><au>Santini, Silvia</au><au>Giovagnoli, Andrea</au><au>Scappini, Lorena</au><au>Minardi, Daniele</au><au>Muzzonigro, Giovanni</au><au>Dessì-Fulgheri, Paolo</au><au>Rappelli, Alessandro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Altered pattern of cannabinoid type 1 receptor expression in adipose tissue of dysmetabolic and overweight patients</atitle><jtitle>Metabolism, clinical and experimental</jtitle><addtitle>Metabolism</addtitle><date>2009-03-01</date><risdate>2009</risdate><volume>58</volume><issue>3</issue><spage>361</spage><epage>367</epage><pages>361-367</pages><issn>0026-0495</issn><eissn>1532-8600</eissn><abstract>Abstract In overweight patients (OW), the increased peripheral activity of the endocannabinoid system in visceral adipose tissue (VAT) may be mediated by cannabinoid type 1 (CB1) receptor expression. We determined whether CB1 receptor splice variants and messenger RNA (mRNA) levels in perirenal and subcutaneous adipose tissues are associated with obesity and metabolic syndrome (MetS). Gene expression with multiple-primers real-time polymerase chain reaction (TaqMan; Applied Biosystem, Weiterstadt, Germany) was performed to study VAT and paired subcutaneous adipose tissue (SAT) mRNA from 36 consecutive patients undergoing nephrectomy. Cannabinoid type 1A and CB1E mRNAs variants with the longer version of exon 4 were expressed. The CB1 expression in perirenal VAT significantly correlated with body mass index (BMI). Paired subcutaneous/perirenal samples from normal-weight patients (BMI <25 kg/m2 ) showed higher CB1 expression in SAT ( P = .002), whereas in OW (BMI ≥25 kg/m2 ), the higher CB1 expression was in VAT ( P = .038). In unpaired samples, SAT of normal-weight patients had significantly higher CB1 mRNA levels compared with SAT of OW, whereas higher CB1 expression ( P = .009) was found in VAT of OW (n = 25). Overweight patients with increased visceral CB1 expression had higher waist circumference ( P < .01), insulin ( P < .01), and homeostasis model assessment index ( P < .01). In addition, patients with the MetS (n = 22) showed higher CB1 expression in perirenal adipose tissues ( P = .007). Visceral adipose CB1 expression correlated with BMI. Overweight patients and those with MetS showed a CB1 expression pattern supporting a CB1-mediated overactivity of the endocannabinoid system in human VAT.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>19217452</pmid><doi>10.1016/j.metabol.2008.10.009</doi><tpages>7</tpages></addata></record> |
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| subjects | Adipose Tissue - physiopathology Alternative Splicing Biological and medical sciences Body Mass Index Body Weight - genetics DNA, Single-Stranded - genetics Endocrinology & Metabolism Exons Feeding. Feeding behavior Fundamental and applied biological sciences. Psychology Gene Expression Regulation Genetic Variation Humans Kidney Neoplasms - genetics Kidney Neoplasms - pathology Medical sciences Metabolic diseases Metabolic Diseases - genetics Metabolic Diseases - physiopathology Metabolic Syndrome - genetics Obesity Obesity - genetics Overweight - genetics Overweight - physiopathology Receptor, Cannabinoid, CB1 - genetics RNA, Messenger - genetics Vertebrates: anatomy and physiology, studies on body, several organs or systems |
| title | Altered pattern of cannabinoid type 1 receptor expression in adipose tissue of dysmetabolic and overweight patients |
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