Relation between cell disruption conditions, cell debris particle size, and inclusion body release

The efficiency of physical separation of inclusion bodies from cell debris is related to cell debris size and inclusion body release and both factors should be taken into account when designing a process. In this work, cell disruption by enzymatic treatment with lysozyme and cellulase, by homogeniza...

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Veröffentlicht in:Biotechnology and bioengineering 2004-10, Vol.88 (1), p.100-110
Hauptverfasser: van Hee, Pim, Middelberg, Anton P.J., van der Lans, Rob G.J.M., van der Wielen, Luuk A.M.
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Sprache:eng
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