Involvement of the Molybdenum Cofactor Biosynthetic Machinery in the Maturation of the Escherichia coli Nitrate Reductase A
The maturation of Escherichia coli nitrate reductase A requires the incorporation of the Mo-(bis-MGD) cofactor to the apoprotein. For this process, the NarJ chaperone is strictly required (Blasco, F., Dos Santos, J. P., Magalon, A., Frixon, C., Guigliarelli, B., Santini, C. L., and Giordano, G. (199...
Gespeichert in:
| Veröffentlicht in: | The Journal of biological chemistry 2004-10, Vol.279 (40), p.41398-41403 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 41403 |
|---|---|
| container_issue | 40 |
| container_start_page | 41398 |
| container_title | The Journal of biological chemistry |
| container_volume | 279 |
| creator | Alexandra Vergnes Kamila Gouffi-Belhabich Francis Blasco Gérard Giordano Axel Magalon |
| description | The maturation of Escherichia coli nitrate reductase A requires the incorporation of the Mo-(bis-MGD) cofactor to the apoprotein. For this process, the NarJ
chaperone is strictly required (Blasco, F., Dos Santos, J. P., Magalon, A., Frixon, C., Guigliarelli, B., Santini, C. L.,
and Giordano, G. (1998) Mol. Microbiol . 28, 435â447). We report the first description of protein interactions between molybdenum cofactor biosynthetic proteins
(MogA, MoeA, MobA, and MobB) and the aponitrate reductase (NarG) using a bacterial two-hybrid approach. Two conditions have
to be satisfied to allow the visualization of the interactions, (i) the presence of an active and mature molybdenum cofactor
and (ii) the presence of the NarJ chaperone and of the NarG structural partner subunit, NarH. Formation of tungsten-substituted
cofactor prevents the interaction between NarG and the four biosynthetic proteins. Our results suggested that the final stages
of molybdenum cofactor biosynthesis occur on a complex made up by MogA, MoeA, MobA, and MobB, which is also in charge with
the delivery of the mature cofactor onto the aponitrate reductase A in a NarJ-assisted process. |
| doi_str_mv | 10.1074/jbc.M407087200 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_66917363</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>17826457</sourcerecordid><originalsourceid>FETCH-LOGICAL-c391t-58393682ddb6d9fc7cc417753aabd25f22ae02b3d555a26aa853389981472b603</originalsourceid><addsrcrecordid>eNqFkU1v1DAQhi0EotvClSPyAXHL4o_461hWBSp1QUIgcbMcZ0JcJXGxnaIVfx5Xu6hH5jKHeeaVZh6EXlGypUS17247v923RBGtGCFP0IYSzRsu6I-naEMIo41hQp-h85xvSa3W0OfojArWKsblBv25Xu7jdA8zLAXHAZcR8D5Oh66HZZ3xLg7Ol5jw-xDzYanTEjzeOz-GBdIBh-W44cqaXAlx-Zdxlf0IKVTOYR-ngD-HUgnAX6FffXEZ8OUL9GxwU4aXp36Bvn-4-rb71Nx8-Xi9u7xpPDe0NEJzw6Vmfd_J3gxeed9SpQR3ruuZGBhzQFjHeyGEY9I5LTjXxmhab-wk4Rfo7TH3LsVfK-Ri55A9TJNbIK7ZSmmo4pL_F6RKM9kKVcHtEfQp5pxgsHcpzC4dLCX2wYutXuyjl7rw-pS8djP0j_hJRAXeHIEx_Bx_hwS2C7G-cLZMGdsS21JuNP8L5IWVgA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17826457</pqid></control><display><type>article</type><title>Involvement of the Molybdenum Cofactor Biosynthetic Machinery in the Maturation of the Escherichia coli Nitrate Reductase A</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Alexandra Vergnes ; Kamila Gouffi-Belhabich ; Francis Blasco ; Gérard Giordano ; Axel Magalon</creator><creatorcontrib>Alexandra Vergnes ; Kamila Gouffi-Belhabich ; Francis Blasco ; Gérard Giordano ; Axel Magalon</creatorcontrib><description>The maturation of Escherichia coli nitrate reductase A requires the incorporation of the Mo-(bis-MGD) cofactor to the apoprotein. For this process, the NarJ
chaperone is strictly required (Blasco, F., Dos Santos, J. P., Magalon, A., Frixon, C., Guigliarelli, B., Santini, C. L.,
and Giordano, G. (1998) Mol. Microbiol . 28, 435â447). We report the first description of protein interactions between molybdenum cofactor biosynthetic proteins
(MogA, MoeA, MobA, and MobB) and the aponitrate reductase (NarG) using a bacterial two-hybrid approach. Two conditions have
to be satisfied to allow the visualization of the interactions, (i) the presence of an active and mature molybdenum cofactor
and (ii) the presence of the NarJ chaperone and of the NarG structural partner subunit, NarH. Formation of tungsten-substituted
cofactor prevents the interaction between NarG and the four biosynthetic proteins. Our results suggested that the final stages
of molybdenum cofactor biosynthesis occur on a complex made up by MogA, MoeA, MobA, and MobB, which is also in charge with
the delivery of the mature cofactor onto the aponitrate reductase A in a NarJ-assisted process.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1074/jbc.M407087200</identifier><identifier>PMID: 15247236</identifier><language>eng</language><publisher>United States: American Society for Biochemistry and Molecular Biology</publisher><subject>Apoproteins - metabolism ; Escherichia coli ; Escherichia coli Proteins - biosynthesis ; Escherichia coli Proteins - metabolism ; Macromolecular Substances ; Molecular Chaperones - metabolism ; Molybdenum ; Nitrate Reductase ; Nitrate Reductases - biosynthesis ; Protein Subunits - metabolism ; Sulfurtransferases - metabolism ; Trans-Activators - metabolism ; Two-Hybrid System Techniques</subject><ispartof>The Journal of biological chemistry, 2004-10, Vol.279 (40), p.41398-41403</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-58393682ddb6d9fc7cc417753aabd25f22ae02b3d555a26aa853389981472b603</citedby><cites>FETCH-LOGICAL-c391t-58393682ddb6d9fc7cc417753aabd25f22ae02b3d555a26aa853389981472b603</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15247236$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Alexandra Vergnes</creatorcontrib><creatorcontrib>Kamila Gouffi-Belhabich</creatorcontrib><creatorcontrib>Francis Blasco</creatorcontrib><creatorcontrib>Gérard Giordano</creatorcontrib><creatorcontrib>Axel Magalon</creatorcontrib><title>Involvement of the Molybdenum Cofactor Biosynthetic Machinery in the Maturation of the Escherichia coli Nitrate Reductase A</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>The maturation of Escherichia coli nitrate reductase A requires the incorporation of the Mo-(bis-MGD) cofactor to the apoprotein. For this process, the NarJ
chaperone is strictly required (Blasco, F., Dos Santos, J. P., Magalon, A., Frixon, C., Guigliarelli, B., Santini, C. L.,
and Giordano, G. (1998) Mol. Microbiol . 28, 435â447). We report the first description of protein interactions between molybdenum cofactor biosynthetic proteins
(MogA, MoeA, MobA, and MobB) and the aponitrate reductase (NarG) using a bacterial two-hybrid approach. Two conditions have
to be satisfied to allow the visualization of the interactions, (i) the presence of an active and mature molybdenum cofactor
and (ii) the presence of the NarJ chaperone and of the NarG structural partner subunit, NarH. Formation of tungsten-substituted
cofactor prevents the interaction between NarG and the four biosynthetic proteins. Our results suggested that the final stages
of molybdenum cofactor biosynthesis occur on a complex made up by MogA, MoeA, MobA, and MobB, which is also in charge with
the delivery of the mature cofactor onto the aponitrate reductase A in a NarJ-assisted process.</description><subject>Apoproteins - metabolism</subject><subject>Escherichia coli</subject><subject>Escherichia coli Proteins - biosynthesis</subject><subject>Escherichia coli Proteins - metabolism</subject><subject>Macromolecular Substances</subject><subject>Molecular Chaperones - metabolism</subject><subject>Molybdenum</subject><subject>Nitrate Reductase</subject><subject>Nitrate Reductases - biosynthesis</subject><subject>Protein Subunits - metabolism</subject><subject>Sulfurtransferases - metabolism</subject><subject>Trans-Activators - metabolism</subject><subject>Two-Hybrid System Techniques</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi0EotvClSPyAXHL4o_461hWBSp1QUIgcbMcZ0JcJXGxnaIVfx5Xu6hH5jKHeeaVZh6EXlGypUS17247v923RBGtGCFP0IYSzRsu6I-naEMIo41hQp-h85xvSa3W0OfojArWKsblBv25Xu7jdA8zLAXHAZcR8D5Oh66HZZ3xLg7Ol5jw-xDzYanTEjzeOz-GBdIBh-W44cqaXAlx-Zdxlf0IKVTOYR-ngD-HUgnAX6FffXEZ8OUL9GxwU4aXp36Bvn-4-rb71Nx8-Xi9u7xpPDe0NEJzw6Vmfd_J3gxeed9SpQR3ruuZGBhzQFjHeyGEY9I5LTjXxmhab-wk4Rfo7TH3LsVfK-Ri55A9TJNbIK7ZSmmo4pL_F6RKM9kKVcHtEfQp5pxgsHcpzC4dLCX2wYutXuyjl7rw-pS8djP0j_hJRAXeHIEx_Bx_hwS2C7G-cLZMGdsS21JuNP8L5IWVgA</recordid><startdate>20041001</startdate><enddate>20041001</enddate><creator>Alexandra Vergnes</creator><creator>Kamila Gouffi-Belhabich</creator><creator>Francis Blasco</creator><creator>Gérard Giordano</creator><creator>Axel Magalon</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20041001</creationdate><title>Involvement of the Molybdenum Cofactor Biosynthetic Machinery in the Maturation of the Escherichia coli Nitrate Reductase A</title><author>Alexandra Vergnes ; Kamila Gouffi-Belhabich ; Francis Blasco ; Gérard Giordano ; Axel Magalon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-58393682ddb6d9fc7cc417753aabd25f22ae02b3d555a26aa853389981472b603</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Apoproteins - metabolism</topic><topic>Escherichia coli</topic><topic>Escherichia coli Proteins - biosynthesis</topic><topic>Escherichia coli Proteins - metabolism</topic><topic>Macromolecular Substances</topic><topic>Molecular Chaperones - metabolism</topic><topic>Molybdenum</topic><topic>Nitrate Reductase</topic><topic>Nitrate Reductases - biosynthesis</topic><topic>Protein Subunits - metabolism</topic><topic>Sulfurtransferases - metabolism</topic><topic>Trans-Activators - metabolism</topic><topic>Two-Hybrid System Techniques</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alexandra Vergnes</creatorcontrib><creatorcontrib>Kamila Gouffi-Belhabich</creatorcontrib><creatorcontrib>Francis Blasco</creatorcontrib><creatorcontrib>Gérard Giordano</creatorcontrib><creatorcontrib>Axel Magalon</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alexandra Vergnes</au><au>Kamila Gouffi-Belhabich</au><au>Francis Blasco</au><au>Gérard Giordano</au><au>Axel Magalon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Involvement of the Molybdenum Cofactor Biosynthetic Machinery in the Maturation of the Escherichia coli Nitrate Reductase A</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>2004-10-01</date><risdate>2004</risdate><volume>279</volume><issue>40</issue><spage>41398</spage><epage>41403</epage><pages>41398-41403</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>The maturation of Escherichia coli nitrate reductase A requires the incorporation of the Mo-(bis-MGD) cofactor to the apoprotein. For this process, the NarJ
chaperone is strictly required (Blasco, F., Dos Santos, J. P., Magalon, A., Frixon, C., Guigliarelli, B., Santini, C. L.,
and Giordano, G. (1998) Mol. Microbiol . 28, 435â447). We report the first description of protein interactions between molybdenum cofactor biosynthetic proteins
(MogA, MoeA, MobA, and MobB) and the aponitrate reductase (NarG) using a bacterial two-hybrid approach. Two conditions have
to be satisfied to allow the visualization of the interactions, (i) the presence of an active and mature molybdenum cofactor
and (ii) the presence of the NarJ chaperone and of the NarG structural partner subunit, NarH. Formation of tungsten-substituted
cofactor prevents the interaction between NarG and the four biosynthetic proteins. Our results suggested that the final stages
of molybdenum cofactor biosynthesis occur on a complex made up by MogA, MoeA, MobA, and MobB, which is also in charge with
the delivery of the mature cofactor onto the aponitrate reductase A in a NarJ-assisted process.</abstract><cop>United States</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>15247236</pmid><doi>10.1074/jbc.M407087200</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9258 |
| ispartof | The Journal of biological chemistry, 2004-10, Vol.279 (40), p.41398-41403 |
| issn | 0021-9258 1083-351X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_66917363 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Apoproteins - metabolism Escherichia coli Escherichia coli Proteins - biosynthesis Escherichia coli Proteins - metabolism Macromolecular Substances Molecular Chaperones - metabolism Molybdenum Nitrate Reductase Nitrate Reductases - biosynthesis Protein Subunits - metabolism Sulfurtransferases - metabolism Trans-Activators - metabolism Two-Hybrid System Techniques |
| title | Involvement of the Molybdenum Cofactor Biosynthetic Machinery in the Maturation of the Escherichia coli Nitrate Reductase A |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-17T20%3A09%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Involvement%20of%20the%20Molybdenum%20Cofactor%20Biosynthetic%20Machinery%20in%20the%20Maturation%20of%20the%20Escherichia%20coli%20Nitrate%20Reductase%20A&rft.jtitle=The%20Journal%20of%20biological%20chemistry&rft.au=Alexandra%20Vergnes&rft.date=2004-10-01&rft.volume=279&rft.issue=40&rft.spage=41398&rft.epage=41403&rft.pages=41398-41403&rft.issn=0021-9258&rft.eissn=1083-351X&rft_id=info:doi/10.1074/jbc.M407087200&rft_dat=%3Cproquest_cross%3E17826457%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17826457&rft_id=info:pmid/15247236&rfr_iscdi=true |