Analysis of Microsatellites and Parentage Testing in Saltwater Crocodiles
Fifteen microsatellite loci were evaluated in farmed saltwater crocodiles for use in parentage testing. One marker (C391) could not be amplified. For the remaining 14, the number of alleles per locus ranged from two to 16, and the observed heterozygosities ranged from 0.219 to 0.875. The cumulative...
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| Veröffentlicht in: | The Journal of heredity 2004-09, Vol.95 (5), p.445-449 |
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| container_title | The Journal of heredity |
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| creator | Isberg, S. R. Chen, Y. Barker, S. G. Moran, C. |
| description | Fifteen microsatellite loci were evaluated in farmed saltwater crocodiles for use in parentage testing. One marker (C391) could not be amplified. For the remaining 14, the number of alleles per locus ranged from two to 16, and the observed heterozygosities ranged from 0.219 to 0.875. The cumulative exclusion probability for all 14 loci was.9988. The 11 loci that showed the greatest level of polymorphism were used for parentage testing, with an exclusion probability of.9980. With these 11 markers on 107 juveniles from 16 known-breeding pairs, a 5.6% pedigree error rate was detected. This level of pedigree error, if consistent, could have an impact on the accuracy of genetic parameter and breeding value estimation. The usefulness of these markers was also evaluated for assigning parentage in situations where maternity, paternity, or both may not be known. In these situations, a 2% error in parentage assignment was predicted. It is therefore recommended that more microsatellite markers be used in these situations. The use of these microsatellite markers will broaden the scope of a breeding program, allowing progeny to be tested from adults maintained in large breeding lagoons for selection as future breeding animals. |
| doi_str_mv | 10.1093/jhered/esh067 |
| format | Article |
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The usefulness of these markers was also evaluated for assigning parentage in situations where maternity, paternity, or both may not be known. In these situations, a 2% error in parentage assignment was predicted. It is therefore recommended that more microsatellite markers be used in these situations. The use of these microsatellite markers will broaden the scope of a breeding program, allowing progeny to be tested from adults maintained in large breeding lagoons for selection as future breeding animals.</description><identifier>ISSN: 0022-1503</identifier><identifier>EISSN: 1465-7333</identifier><identifier>DOI: 10.1093/jhered/esh067</identifier><identifier>PMID: 15388772</identifier><identifier>CODEN: JOHEA8</identifier><language>eng</language><publisher>United States: Oxford University Press</publisher><subject>Alligators and Crocodiles - genetics ; Animals ; Brackish ; Breeding - methods ; Breeding of animals ; Crocodiles ; Crocodylus ; Crocodylus porosus ; DNA Primers ; Gene Frequency ; Gene loci ; Genetic Carrier Screening ; Genetic markers ; Genetics, Population ; Linkage Disequilibrium - genetics ; Marine ; Microsatellite Repeats - genetics ; Northern Territory ; Pedigree ; Polymorphism, Genetic</subject><ispartof>The Journal of heredity, 2004-09, Vol.95 (5), p.445-449</ispartof><rights>Copyright Oxford University Press(England) Sep/Oct 2004</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c424t-63e491a51a9ae8f0af7519e3b9b3cefbb07ab479aca255292b757d9263beac93</citedby><cites>FETCH-LOGICAL-c424t-63e491a51a9ae8f0af7519e3b9b3cefbb07ab479aca255292b757d9263beac93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15388772$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Isberg, S. R.</creatorcontrib><creatorcontrib>Chen, Y.</creatorcontrib><creatorcontrib>Barker, S. G.</creatorcontrib><creatorcontrib>Moran, C.</creatorcontrib><title>Analysis of Microsatellites and Parentage Testing in Saltwater Crocodiles</title><title>The Journal of heredity</title><addtitle>J Hered</addtitle><description>Fifteen microsatellite loci were evaluated in farmed saltwater crocodiles for use in parentage testing. One marker (C391) could not be amplified. For the remaining 14, the number of alleles per locus ranged from two to 16, and the observed heterozygosities ranged from 0.219 to 0.875. The cumulative exclusion probability for all 14 loci was.9988. The 11 loci that showed the greatest level of polymorphism were used for parentage testing, with an exclusion probability of.9980. With these 11 markers on 107 juveniles from 16 known-breeding pairs, a 5.6% pedigree error rate was detected. This level of pedigree error, if consistent, could have an impact on the accuracy of genetic parameter and breeding value estimation. The usefulness of these markers was also evaluated for assigning parentage in situations where maternity, paternity, or both may not be known. In these situations, a 2% error in parentage assignment was predicted. It is therefore recommended that more microsatellite markers be used in these situations. The use of these microsatellite markers will broaden the scope of a breeding program, allowing progeny to be tested from adults maintained in large breeding lagoons for selection as future breeding animals.</description><subject>Alligators and Crocodiles - genetics</subject><subject>Animals</subject><subject>Brackish</subject><subject>Breeding - methods</subject><subject>Breeding of animals</subject><subject>Crocodiles</subject><subject>Crocodylus</subject><subject>Crocodylus porosus</subject><subject>DNA Primers</subject><subject>Gene Frequency</subject><subject>Gene loci</subject><subject>Genetic Carrier Screening</subject><subject>Genetic markers</subject><subject>Genetics, Population</subject><subject>Linkage Disequilibrium - genetics</subject><subject>Marine</subject><subject>Microsatellite Repeats - genetics</subject><subject>Northern Territory</subject><subject>Pedigree</subject><subject>Polymorphism, Genetic</subject><issn>0022-1503</issn><issn>1465-7333</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0c9LwzAUB_Agis7p0asUD96qSdMkzVHmjwmKwnYQL-G1e52ZXatJi-6_N6NjghdPgeSTL4_3JeSE0QtGNb9cvKHD2SX6NyrVDhmwVIpYcc53yYDSJImZoPyAHHq_oJQyoek-OWCCZ5lSyYDcX9VQrbz1UVNGj7ZwjYcWq8q26COoZ9EzOKxbmGM0Rd_aeh7ZOppA1X4F56KRa4pmZiv0R2SvhMrj8eYckuntzXQ0jh-e7u5HVw9xkSZpG0uOqWYgGGjArKRQKsE08lznvMAyz6mCPFUaCkiESHSSK6FmOpE8Ryg0H5LzPvbDNZ9dGMksrS_CxFBj03kjpaYZo-xfyCRTmnEV4NkfuGg6F9YSjF5HZTINKO7RekPeYWk-nF2CWxlGzboI0xdh-iKCP92EdvkyXG_1ZvO_gda3-L19B_duwnclzPjlNWiZPo8nzFzzH28flL8</recordid><startdate>200409</startdate><enddate>200409</enddate><creator>Isberg, S. R.</creator><creator>Chen, Y.</creator><creator>Barker, S. G.</creator><creator>Moran, C.</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7SN</scope><scope>7SS</scope><scope>7TK</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>200409</creationdate><title>Analysis of Microsatellites and Parentage Testing in Saltwater Crocodiles</title><author>Isberg, S. R. ; Chen, Y. ; Barker, S. G. ; Moran, C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c424t-63e491a51a9ae8f0af7519e3b9b3cefbb07ab479aca255292b757d9263beac93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Alligators and Crocodiles - genetics</topic><topic>Animals</topic><topic>Brackish</topic><topic>Breeding - methods</topic><topic>Breeding of animals</topic><topic>Crocodiles</topic><topic>Crocodylus</topic><topic>Crocodylus porosus</topic><topic>DNA Primers</topic><topic>Gene Frequency</topic><topic>Gene loci</topic><topic>Genetic Carrier Screening</topic><topic>Genetic markers</topic><topic>Genetics, Population</topic><topic>Linkage Disequilibrium - genetics</topic><topic>Marine</topic><topic>Microsatellite Repeats - genetics</topic><topic>Northern Territory</topic><topic>Pedigree</topic><topic>Polymorphism, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Isberg, S. 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R.</au><au>Chen, Y.</au><au>Barker, S. G.</au><au>Moran, C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of Microsatellites and Parentage Testing in Saltwater Crocodiles</atitle><jtitle>The Journal of heredity</jtitle><addtitle>J Hered</addtitle><date>2004-09</date><risdate>2004</risdate><volume>95</volume><issue>5</issue><spage>445</spage><epage>449</epage><pages>445-449</pages><issn>0022-1503</issn><eissn>1465-7333</eissn><coden>JOHEA8</coden><abstract>Fifteen microsatellite loci were evaluated in farmed saltwater crocodiles for use in parentage testing. One marker (C391) could not be amplified. For the remaining 14, the number of alleles per locus ranged from two to 16, and the observed heterozygosities ranged from 0.219 to 0.875. The cumulative exclusion probability for all 14 loci was.9988. The 11 loci that showed the greatest level of polymorphism were used for parentage testing, with an exclusion probability of.9980. With these 11 markers on 107 juveniles from 16 known-breeding pairs, a 5.6% pedigree error rate was detected. This level of pedigree error, if consistent, could have an impact on the accuracy of genetic parameter and breeding value estimation. The usefulness of these markers was also evaluated for assigning parentage in situations where maternity, paternity, or both may not be known. In these situations, a 2% error in parentage assignment was predicted. It is therefore recommended that more microsatellite markers be used in these situations. The use of these microsatellite markers will broaden the scope of a breeding program, allowing progeny to be tested from adults maintained in large breeding lagoons for selection as future breeding animals.</abstract><cop>United States</cop><pub>Oxford University Press</pub><pmid>15388772</pmid><doi>10.1093/jhered/esh067</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Alligators and Crocodiles - genetics Animals Brackish Breeding - methods Breeding of animals Crocodiles Crocodylus Crocodylus porosus DNA Primers Gene Frequency Gene loci Genetic Carrier Screening Genetic markers Genetics, Population Linkage Disequilibrium - genetics Marine Microsatellite Repeats - genetics Northern Territory Pedigree Polymorphism, Genetic |
| title | Analysis of Microsatellites and Parentage Testing in Saltwater Crocodiles |
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