Inhibitory effects of mulberry leaf extract on postprandial hyperglycemia in normal rats
We examined the inhibitory effects of aqueous ethanol extract from mulberry leaves (ME) on postprandial hyperglycemia in normal Wistar rats. ME dose-dependently suppressed the postprandial rise of blood glucose in rats, when ME (0.02-0.5g/kg) was given 0.5h before the administration of carbohydrates...
Gespeichert in:
| Veröffentlicht in: | Journal of Nutritional Science and Vitaminology 2004, Vol.50(3), pp.161-164 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 164 |
|---|---|
| container_issue | 3 |
| container_start_page | 161 |
| container_title | Journal of Nutritional Science and Vitaminology |
| container_volume | 50 |
| creator | Miyahara, C. (Kanagawa-ken. Inst. of Public Health, Chigasaki (Japan)) Miyazawa, M Satoh, S Sakai, A Mizusaki, S |
| description | We examined the inhibitory effects of aqueous ethanol extract from mulberry leaves (ME) on postprandial hyperglycemia in normal Wistar rats. ME dose-dependently suppressed the postprandial rise of blood glucose in rats, when ME (0.02-0.5g/kg) was given 0.5h before the administration of carbohydrates such as sucrose, maltose and starch. The ME dose showing 50% inhibition of the increment of blood glucose (ED50) was 0.11g/kg for sucrose, 0.44g/kg for maltose, and 0.38g/kg for starch. ME and its basic fraction (MB) containing 1-deoxynojirimycin were assayed for their inhibitory effects (IC50) on disaccharidase derived from the small intestine of rats. The IC50 value of ME was 3.2μg/mL for sucrase, 10μg/mL for isomaltase, and 51μg/mL for maltase. The IC50 value of MB was 0.36μg/mL for sucrase, 1.1μg/mL for isomaltase, and 6.2μg/mL for maltase. The IC50 value of 1-deoxynojirimycin as the principle component in ME was 0.015μg/mL for sucrase and 0.21μg/ mL for maltase, and this value was comparable to the IC50 of voglibose. The inhibitory activity of ME in α-amylase was weak. These results suggest that ME strongly suppresses postprandial hyperglycemia after carbohydrate loading by inhibiting the activity of disaccharidases in the small intestine of rats. |
| doi_str_mv | 10.3177/jnsv.50.161 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_66907675</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>66907675</sourcerecordid><originalsourceid>FETCH-LOGICAL-c607t-91229584ef1caa0ce21b65996f2780e713cef83d065950ab40e32eb96888c3213</originalsourceid><addsrcrecordid>eNpFkE1vEzEURS0EomlhxRo0G9igCc_2jO1ZVoVCUSVYgMTOeuM8JxPNF7aDyL_HIaN2Y0v3Hd0rHcZecVhLrvWH_Rj_rGtYc8WfsBU3hpdaV-IpW4EEXlYG4IJdxrgHqBpTmefsgtfSqEboFft1N-66tktTOBbkPbkUi8kXw6FvKeSsJ_QF_U0BXSqmsZinmOaA46bDvtgdZwrb_uho6LDoxmKcwpDzgCm-YM889pFeLv8V-3n76cfNl_L-2-e7m-v70inQqWy4EE1tKvLcIYIjwVtVN43yQhsgzaUjb-QGclgDthWQFNQ2yhjjpODyir07985h-n2gmOzQRUd9jyNNh2iVakArXWfw_Rl0YYoxkLdz6AYMR8vBnkTak0hbg80iM_1mqT20A20e2cVcBt4uAEaHvc9OXBcfOQWVECAz9_HM7WPCLT0AGFLnevo_yhstT8PLk_cfzm6HwdKYa16fazxOFrchT339LgBqAC6FlP8ARy-dRQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>66907675</pqid></control><display><type>article</type><title>Inhibitory effects of mulberry leaf extract on postprandial hyperglycemia in normal rats</title><source>J-STAGE Free</source><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Miyahara, C. (Kanagawa-ken. Inst. of Public Health, Chigasaki (Japan)) ; Miyazawa, M ; Satoh, S ; Sakai, A ; Mizusaki, S</creator><creatorcontrib>Miyahara, C. (Kanagawa-ken. Inst. of Public Health, Chigasaki (Japan)) ; Miyazawa, M ; Satoh, S ; Sakai, A ; Mizusaki, S</creatorcontrib><description>We examined the inhibitory effects of aqueous ethanol extract from mulberry leaves (ME) on postprandial hyperglycemia in normal Wistar rats. ME dose-dependently suppressed the postprandial rise of blood glucose in rats, when ME (0.02-0.5g/kg) was given 0.5h before the administration of carbohydrates such as sucrose, maltose and starch. The ME dose showing 50% inhibition of the increment of blood glucose (ED50) was 0.11g/kg for sucrose, 0.44g/kg for maltose, and 0.38g/kg for starch. ME and its basic fraction (MB) containing 1-deoxynojirimycin were assayed for their inhibitory effects (IC50) on disaccharidase derived from the small intestine of rats. The IC50 value of ME was 3.2μg/mL for sucrase, 10μg/mL for isomaltase, and 51μg/mL for maltase. The IC50 value of MB was 0.36μg/mL for sucrase, 1.1μg/mL for isomaltase, and 6.2μg/mL for maltase. The IC50 value of 1-deoxynojirimycin as the principle component in ME was 0.015μg/mL for sucrase and 0.21μg/ mL for maltase, and this value was comparable to the IC50 of voglibose. The inhibitory activity of ME in α-amylase was weak. These results suggest that ME strongly suppresses postprandial hyperglycemia after carbohydrate loading by inhibiting the activity of disaccharidases in the small intestine of rats.</description><identifier>ISSN: 0301-4800</identifier><identifier>EISSN: 1881-7742</identifier><identifier>DOI: 10.3177/jnsv.50.161</identifier><identifier>PMID: 15386927</identifier><language>eng</language><publisher>Tokyo: Center for Academic Publications Japan</publisher><subject><![CDATA[1-deoxynojirimycin ; 1-Deoxynojirimycin - pharmacology ; AMYLASES ; Animals ; antipostprandial hyperglycemia ; Biological and medical sciences ; DIABETES ; Dietary Carbohydrates - administration & dosage ; Dietary Sucrose - administration & dosage ; disaccharidase inhibitor ; Disaccharidases - antagonists & inhibitors ; Enzyme Inhibitors - pharmacology ; EXTRACTS ; Feeding. Feeding behavior ; Food ; Fundamental and applied biological sciences. Psychology ; GLUCOSIDASES ; Glycoside Hydrolase Inhibitors ; Hyperglycemia - prevention & control ; Intestine, Small - enzymology ; LEAVES ; Maltose - administration & dosage ; Morus - chemistry ; MULBERRIES ; mulberry leaf ; Oligo-1,6-Glucosidase - antagonists & inhibitors ; Plant Extracts - administration & dosage ; Plant Leaves - chemistry ; Rats ; Rats, Wistar ; Starch - administration & dosage ; Sucrase - antagonists & inhibitors ; Vertebrates: anatomy and physiology, studies on body, several organs or systems ; Wistar rat]]></subject><ispartof>Journal of Nutritional Science and Vitaminology, 2004, Vol.50(3), pp.161-164</ispartof><rights>the Center for Academic Publications Japan</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c607t-91229584ef1caa0ce21b65996f2780e713cef83d065950ab40e32eb96888c3213</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16042203$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15386927$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Miyahara, C. (Kanagawa-ken. Inst. of Public Health, Chigasaki (Japan))</creatorcontrib><creatorcontrib>Miyazawa, M</creatorcontrib><creatorcontrib>Satoh, S</creatorcontrib><creatorcontrib>Sakai, A</creatorcontrib><creatorcontrib>Mizusaki, S</creatorcontrib><title>Inhibitory effects of mulberry leaf extract on postprandial hyperglycemia in normal rats</title><title>Journal of Nutritional Science and Vitaminology</title><addtitle>J Nutr Sci Vitaminol</addtitle><description>We examined the inhibitory effects of aqueous ethanol extract from mulberry leaves (ME) on postprandial hyperglycemia in normal Wistar rats. ME dose-dependently suppressed the postprandial rise of blood glucose in rats, when ME (0.02-0.5g/kg) was given 0.5h before the administration of carbohydrates such as sucrose, maltose and starch. The ME dose showing 50% inhibition of the increment of blood glucose (ED50) was 0.11g/kg for sucrose, 0.44g/kg for maltose, and 0.38g/kg for starch. ME and its basic fraction (MB) containing 1-deoxynojirimycin were assayed for their inhibitory effects (IC50) on disaccharidase derived from the small intestine of rats. The IC50 value of ME was 3.2μg/mL for sucrase, 10μg/mL for isomaltase, and 51μg/mL for maltase. The IC50 value of MB was 0.36μg/mL for sucrase, 1.1μg/mL for isomaltase, and 6.2μg/mL for maltase. The IC50 value of 1-deoxynojirimycin as the principle component in ME was 0.015μg/mL for sucrase and 0.21μg/ mL for maltase, and this value was comparable to the IC50 of voglibose. The inhibitory activity of ME in α-amylase was weak. These results suggest that ME strongly suppresses postprandial hyperglycemia after carbohydrate loading by inhibiting the activity of disaccharidases in the small intestine of rats.</description><subject>1-deoxynojirimycin</subject><subject>1-Deoxynojirimycin - pharmacology</subject><subject>AMYLASES</subject><subject>Animals</subject><subject>antipostprandial hyperglycemia</subject><subject>Biological and medical sciences</subject><subject>DIABETES</subject><subject>Dietary Carbohydrates - administration & dosage</subject><subject>Dietary Sucrose - administration & dosage</subject><subject>disaccharidase inhibitor</subject><subject>Disaccharidases - antagonists & inhibitors</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>EXTRACTS</subject><subject>Feeding. Feeding behavior</subject><subject>Food</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GLUCOSIDASES</subject><subject>Glycoside Hydrolase Inhibitors</subject><subject>Hyperglycemia - prevention & control</subject><subject>Intestine, Small - enzymology</subject><subject>LEAVES</subject><subject>Maltose - administration & dosage</subject><subject>Morus - chemistry</subject><subject>MULBERRIES</subject><subject>mulberry leaf</subject><subject>Oligo-1,6-Glucosidase - antagonists & inhibitors</subject><subject>Plant Extracts - administration & dosage</subject><subject>Plant Leaves - chemistry</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Starch - administration & dosage</subject><subject>Sucrase - antagonists & inhibitors</subject><subject>Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><subject>Wistar rat</subject><issn>0301-4800</issn><issn>1881-7742</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkE1vEzEURS0EomlhxRo0G9igCc_2jO1ZVoVCUSVYgMTOeuM8JxPNF7aDyL_HIaN2Y0v3Hd0rHcZecVhLrvWH_Rj_rGtYc8WfsBU3hpdaV-IpW4EEXlYG4IJdxrgHqBpTmefsgtfSqEboFft1N-66tktTOBbkPbkUi8kXw6FvKeSsJ_QF_U0BXSqmsZinmOaA46bDvtgdZwrb_uho6LDoxmKcwpDzgCm-YM889pFeLv8V-3n76cfNl_L-2-e7m-v70inQqWy4EE1tKvLcIYIjwVtVN43yQhsgzaUjb-QGclgDthWQFNQ2yhjjpODyir07985h-n2gmOzQRUd9jyNNh2iVakArXWfw_Rl0YYoxkLdz6AYMR8vBnkTak0hbg80iM_1mqT20A20e2cVcBt4uAEaHvc9OXBcfOQWVECAz9_HM7WPCLT0AGFLnevo_yhstT8PLk_cfzm6HwdKYa16fazxOFrchT339LgBqAC6FlP8ARy-dRQ</recordid><startdate>2004</startdate><enddate>2004</enddate><creator>Miyahara, C. (Kanagawa-ken. Inst. of Public Health, Chigasaki (Japan))</creator><creator>Miyazawa, M</creator><creator>Satoh, S</creator><creator>Sakai, A</creator><creator>Mizusaki, S</creator><general>Center for Academic Publications Japan</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2004</creationdate><title>Inhibitory effects of mulberry leaf extract on postprandial hyperglycemia in normal rats</title><author>Miyahara, C. (Kanagawa-ken. Inst. of Public Health, Chigasaki (Japan)) ; Miyazawa, M ; Satoh, S ; Sakai, A ; Mizusaki, S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c607t-91229584ef1caa0ce21b65996f2780e713cef83d065950ab40e32eb96888c3213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>1-deoxynojirimycin</topic><topic>1-Deoxynojirimycin - pharmacology</topic><topic>AMYLASES</topic><topic>Animals</topic><topic>antipostprandial hyperglycemia</topic><topic>Biological and medical sciences</topic><topic>DIABETES</topic><topic>Dietary Carbohydrates - administration & dosage</topic><topic>Dietary Sucrose - administration & dosage</topic><topic>disaccharidase inhibitor</topic><topic>Disaccharidases - antagonists & inhibitors</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>EXTRACTS</topic><topic>Feeding. Feeding behavior</topic><topic>Food</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GLUCOSIDASES</topic><topic>Glycoside Hydrolase Inhibitors</topic><topic>Hyperglycemia - prevention & control</topic><topic>Intestine, Small - enzymology</topic><topic>LEAVES</topic><topic>Maltose - administration & dosage</topic><topic>Morus - chemistry</topic><topic>MULBERRIES</topic><topic>mulberry leaf</topic><topic>Oligo-1,6-Glucosidase - antagonists & inhibitors</topic><topic>Plant Extracts - administration & dosage</topic><topic>Plant Leaves - chemistry</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Starch - administration & dosage</topic><topic>Sucrase - antagonists & inhibitors</topic><topic>Vertebrates: anatomy and physiology, studies on body, several organs or systems</topic><topic>Wistar rat</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Miyahara, C. (Kanagawa-ken. Inst. of Public Health, Chigasaki (Japan))</creatorcontrib><creatorcontrib>Miyazawa, M</creatorcontrib><creatorcontrib>Satoh, S</creatorcontrib><creatorcontrib>Sakai, A</creatorcontrib><creatorcontrib>Mizusaki, S</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Nutritional Science and Vitaminology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Miyahara, C. (Kanagawa-ken. Inst. of Public Health, Chigasaki (Japan))</au><au>Miyazawa, M</au><au>Satoh, S</au><au>Sakai, A</au><au>Mizusaki, S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibitory effects of mulberry leaf extract on postprandial hyperglycemia in normal rats</atitle><jtitle>Journal of Nutritional Science and Vitaminology</jtitle><addtitle>J Nutr Sci Vitaminol</addtitle><date>2004</date><risdate>2004</risdate><volume>50</volume><issue>3</issue><spage>161</spage><epage>164</epage><pages>161-164</pages><issn>0301-4800</issn><eissn>1881-7742</eissn><abstract>We examined the inhibitory effects of aqueous ethanol extract from mulberry leaves (ME) on postprandial hyperglycemia in normal Wistar rats. ME dose-dependently suppressed the postprandial rise of blood glucose in rats, when ME (0.02-0.5g/kg) was given 0.5h before the administration of carbohydrates such as sucrose, maltose and starch. The ME dose showing 50% inhibition of the increment of blood glucose (ED50) was 0.11g/kg for sucrose, 0.44g/kg for maltose, and 0.38g/kg for starch. ME and its basic fraction (MB) containing 1-deoxynojirimycin were assayed for their inhibitory effects (IC50) on disaccharidase derived from the small intestine of rats. The IC50 value of ME was 3.2μg/mL for sucrase, 10μg/mL for isomaltase, and 51μg/mL for maltase. The IC50 value of MB was 0.36μg/mL for sucrase, 1.1μg/mL for isomaltase, and 6.2μg/mL for maltase. The IC50 value of 1-deoxynojirimycin as the principle component in ME was 0.015μg/mL for sucrase and 0.21μg/ mL for maltase, and this value was comparable to the IC50 of voglibose. The inhibitory activity of ME in α-amylase was weak. These results suggest that ME strongly suppresses postprandial hyperglycemia after carbohydrate loading by inhibiting the activity of disaccharidases in the small intestine of rats.</abstract><cop>Tokyo</cop><pub>Center for Academic Publications Japan</pub><pmid>15386927</pmid><doi>10.3177/jnsv.50.161</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0301-4800 |
| ispartof | Journal of Nutritional Science and Vitaminology, 2004, Vol.50(3), pp.161-164 |
| issn | 0301-4800 1881-7742 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_66907675 |
| source | J-STAGE Free; MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | 1-deoxynojirimycin 1-Deoxynojirimycin - pharmacology AMYLASES Animals antipostprandial hyperglycemia Biological and medical sciences DIABETES Dietary Carbohydrates - administration & dosage Dietary Sucrose - administration & dosage disaccharidase inhibitor Disaccharidases - antagonists & inhibitors Enzyme Inhibitors - pharmacology EXTRACTS Feeding. Feeding behavior Food Fundamental and applied biological sciences. Psychology GLUCOSIDASES Glycoside Hydrolase Inhibitors Hyperglycemia - prevention & control Intestine, Small - enzymology LEAVES Maltose - administration & dosage Morus - chemistry MULBERRIES mulberry leaf Oligo-1,6-Glucosidase - antagonists & inhibitors Plant Extracts - administration & dosage Plant Leaves - chemistry Rats Rats, Wistar Starch - administration & dosage Sucrase - antagonists & inhibitors Vertebrates: anatomy and physiology, studies on body, several organs or systems Wistar rat |
| title | Inhibitory effects of mulberry leaf extract on postprandial hyperglycemia in normal rats |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-30T02%3A49%3A01IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Inhibitory%20effects%20of%20mulberry%20leaf%20extract%20on%20postprandial%20hyperglycemia%20in%20normal%20rats&rft.jtitle=Journal%20of%20Nutritional%20Science%20and%20Vitaminology&rft.au=Miyahara,%20C.%20(Kanagawa-ken.%20Inst.%20of%20Public%20Health,%20Chigasaki%20(Japan))&rft.date=2004&rft.volume=50&rft.issue=3&rft.spage=161&rft.epage=164&rft.pages=161-164&rft.issn=0301-4800&rft.eissn=1881-7742&rft_id=info:doi/10.3177/jnsv.50.161&rft_dat=%3Cproquest_cross%3E66907675%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=66907675&rft_id=info:pmid/15386927&rfr_iscdi=true |