Is the ClC-2 chloride channel involved in the Cl − secretory mechanism of gastric parietal cells?
It has been controversial whether the ClC-2 chloride channel is involved in hydrochloric acid secretion of gastric parietal cells. Here, we investigated whether ClC-2 is the apical Cl − channel associated with gastric acid secretion. Two anti-ClC-2 antibodies used in this study reacted with cloned C...
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| Veröffentlicht in: | FEBS letters 2004-09, Vol.575 (1), p.105-108 |
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| creator | Hori, Katsuhito Takahashi, Yuji Horikawa, Naoki Furukawa, Tetsushi Tsukada, Kazuhiro Takeguchi, Noriaki Sakai, Hideki |
| description | It has been controversial whether the ClC-2 chloride channel is involved in hydrochloric acid secretion of gastric parietal cells. Here, we investigated whether ClC-2 is the apical Cl
− channel associated with gastric acid secretion. Two anti-ClC-2 antibodies used in this study reacted with cloned ClC-2 protein expressed in HEK293 cells. In isolated rabbit gastric glands, significant expression of ClC-2 mRNA was observed, but the presence of ClC-2 protein was not clear. Furthermore, no expression of ClC-2 protein was observed in isolated rat and human gastric mucosa. Immunohistochemistry on the rat gastric mucosa showed no significant expression of ClC-2 protein in the parietal cells which showed abundant expression of H
+,K
+-ATPase. These results indicate that ClC-2 may not be a Cl
−-transporting protein for gastric acid secretion in parietal cells. |
| doi_str_mv | 10.1016/j.febslet.2004.08.044 |
| format | Article |
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− channel associated with gastric acid secretion. Two anti-ClC-2 antibodies used in this study reacted with cloned ClC-2 protein expressed in HEK293 cells. In isolated rabbit gastric glands, significant expression of ClC-2 mRNA was observed, but the presence of ClC-2 protein was not clear. Furthermore, no expression of ClC-2 protein was observed in isolated rat and human gastric mucosa. Immunohistochemistry on the rat gastric mucosa showed no significant expression of ClC-2 protein in the parietal cells which showed abundant expression of H
+,K
+-ATPase. These results indicate that ClC-2 may not be a Cl
−-transporting protein for gastric acid secretion in parietal cells.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/j.febslet.2004.08.044</identifier><identifier>PMID: 15388342</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Aged ; Animals ; Cell Line ; Chloride channel ; Chloride Channels - genetics ; Chloride Channels - metabolism ; Chlorides - metabolism ; ClC-2 ; Female ; Gastric acid ; Gastric Acid - secretion ; Gastric Mucosa - cytology ; Gastric Mucosa - metabolism ; H(+)-K(+)-Exchanging ATPase - genetics ; H(+)-K(+)-Exchanging ATPase - metabolism ; Humans ; Male ; Middle Aged ; Parietal Cells, Gastric - cytology ; Parietal Cells, Gastric - secretion ; Rabbits ; Rats ; RNA, Messenger - metabolism ; Stomach - anatomy & histology ; Stomach - metabolism</subject><ispartof>FEBS letters, 2004-09, Vol.575 (1), p.105-108</ispartof><rights>2004 Federation of European Biochemical Societies</rights><rights>FEBS Letters 575 (2004) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4922-d770aaf1e5621b981c19cdf8ece67a039fc58f3a948e2ee34cb2eddfaee38c343</citedby><cites>FETCH-LOGICAL-c4922-d770aaf1e5621b981c19cdf8ece67a039fc58f3a948e2ee34cb2eddfaee38c343</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2Fj.febslet.2004.08.044$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0014579304010622$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,1411,1427,3536,27902,27903,45552,45553,46386,46810,65308</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15388342$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hori, Katsuhito</creatorcontrib><creatorcontrib>Takahashi, Yuji</creatorcontrib><creatorcontrib>Horikawa, Naoki</creatorcontrib><creatorcontrib>Furukawa, Tetsushi</creatorcontrib><creatorcontrib>Tsukada, Kazuhiro</creatorcontrib><creatorcontrib>Takeguchi, Noriaki</creatorcontrib><creatorcontrib>Sakai, Hideki</creatorcontrib><title>Is the ClC-2 chloride channel involved in the Cl − secretory mechanism of gastric parietal cells?</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>It has been controversial whether the ClC-2 chloride channel is involved in hydrochloric acid secretion of gastric parietal cells. Here, we investigated whether ClC-2 is the apical Cl
− channel associated with gastric acid secretion. Two anti-ClC-2 antibodies used in this study reacted with cloned ClC-2 protein expressed in HEK293 cells. In isolated rabbit gastric glands, significant expression of ClC-2 mRNA was observed, but the presence of ClC-2 protein was not clear. Furthermore, no expression of ClC-2 protein was observed in isolated rat and human gastric mucosa. Immunohistochemistry on the rat gastric mucosa showed no significant expression of ClC-2 protein in the parietal cells which showed abundant expression of H
+,K
+-ATPase. These results indicate that ClC-2 may not be a Cl
−-transporting protein for gastric acid secretion in parietal cells.</description><subject>Aged</subject><subject>Animals</subject><subject>Cell Line</subject><subject>Chloride channel</subject><subject>Chloride Channels - genetics</subject><subject>Chloride Channels - metabolism</subject><subject>Chlorides - metabolism</subject><subject>ClC-2</subject><subject>Female</subject><subject>Gastric acid</subject><subject>Gastric Acid - secretion</subject><subject>Gastric Mucosa - cytology</subject><subject>Gastric Mucosa - metabolism</subject><subject>H(+)-K(+)-Exchanging ATPase - genetics</subject><subject>H(+)-K(+)-Exchanging ATPase - metabolism</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Parietal Cells, Gastric - cytology</subject><subject>Parietal Cells, Gastric - secretion</subject><subject>Rabbits</subject><subject>Rats</subject><subject>RNA, Messenger - metabolism</subject><subject>Stomach - anatomy & histology</subject><subject>Stomach - metabolism</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUtu2zAQhokiQeOkPUIDrrKTypckahW0hvMAAmSRdk3Q5LChQUkOKTvwDbLuEXuSUrCALpsVh8A3_wy-QegLJSUltP66KR2sU4CxZISIksiSCPEBLahseMFFLU_QghAqiqpp-Rk6T2lD8l_S9iM6oxWXkgu2QOY-4fEZ8DIsC4bNcxiit5AL3fcQsO_3Q9iDzcWM4T9vv3ECE2Ec4gF3MKE-dXhw-JdOY_QGb3X0MOqADYSQrj-hU6dDgs_ze4F-3qx-LO-Kh8fb--W3h8KIlrHCNg3R2lGoakbXraSGtsY6CQbqRhPeOlNJx3UrJDAALsyagbVO51oaLvgFujrmbuPwsoM0qs6naQXdw7BLqq5bIqRoMlgdQROHlCI4tY2-0_GgKFGTXbVRs1012VVEqmw3913OA3brDuy_rllnBu6OwKsPcHhfqrpZfWdP06mmSxFBKKnZFHV9jIJsbO8hqmQ89Aasj2BGZQf_n23_AvbmpSA</recordid><startdate>20040924</startdate><enddate>20040924</enddate><creator>Hori, Katsuhito</creator><creator>Takahashi, Yuji</creator><creator>Horikawa, Naoki</creator><creator>Furukawa, Tetsushi</creator><creator>Tsukada, Kazuhiro</creator><creator>Takeguchi, Noriaki</creator><creator>Sakai, Hideki</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20040924</creationdate><title>Is the ClC-2 chloride channel involved in the Cl − secretory mechanism of gastric parietal cells?</title><author>Hori, Katsuhito ; Takahashi, Yuji ; Horikawa, Naoki ; Furukawa, Tetsushi ; Tsukada, Kazuhiro ; Takeguchi, Noriaki ; Sakai, Hideki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4922-d770aaf1e5621b981c19cdf8ece67a039fc58f3a948e2ee34cb2eddfaee38c343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Aged</topic><topic>Animals</topic><topic>Cell Line</topic><topic>Chloride channel</topic><topic>Chloride Channels - genetics</topic><topic>Chloride Channels - metabolism</topic><topic>Chlorides - metabolism</topic><topic>ClC-2</topic><topic>Female</topic><topic>Gastric acid</topic><topic>Gastric Acid - secretion</topic><topic>Gastric Mucosa - cytology</topic><topic>Gastric Mucosa - metabolism</topic><topic>H(+)-K(+)-Exchanging ATPase - genetics</topic><topic>H(+)-K(+)-Exchanging ATPase - metabolism</topic><topic>Humans</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Parietal Cells, Gastric - cytology</topic><topic>Parietal Cells, Gastric - secretion</topic><topic>Rabbits</topic><topic>Rats</topic><topic>RNA, Messenger - metabolism</topic><topic>Stomach - anatomy & histology</topic><topic>Stomach - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hori, Katsuhito</creatorcontrib><creatorcontrib>Takahashi, Yuji</creatorcontrib><creatorcontrib>Horikawa, Naoki</creatorcontrib><creatorcontrib>Furukawa, Tetsushi</creatorcontrib><creatorcontrib>Tsukada, Kazuhiro</creatorcontrib><creatorcontrib>Takeguchi, Noriaki</creatorcontrib><creatorcontrib>Sakai, Hideki</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hori, Katsuhito</au><au>Takahashi, Yuji</au><au>Horikawa, Naoki</au><au>Furukawa, Tetsushi</au><au>Tsukada, Kazuhiro</au><au>Takeguchi, Noriaki</au><au>Sakai, Hideki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Is the ClC-2 chloride channel involved in the Cl − secretory mechanism of gastric parietal cells?</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2004-09-24</date><risdate>2004</risdate><volume>575</volume><issue>1</issue><spage>105</spage><epage>108</epage><pages>105-108</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>It has been controversial whether the ClC-2 chloride channel is involved in hydrochloric acid secretion of gastric parietal cells. Here, we investigated whether ClC-2 is the apical Cl
− channel associated with gastric acid secretion. Two anti-ClC-2 antibodies used in this study reacted with cloned ClC-2 protein expressed in HEK293 cells. In isolated rabbit gastric glands, significant expression of ClC-2 mRNA was observed, but the presence of ClC-2 protein was not clear. Furthermore, no expression of ClC-2 protein was observed in isolated rat and human gastric mucosa. Immunohistochemistry on the rat gastric mucosa showed no significant expression of ClC-2 protein in the parietal cells which showed abundant expression of H
+,K
+-ATPase. These results indicate that ClC-2 may not be a Cl
−-transporting protein for gastric acid secretion in parietal cells.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>15388342</pmid><doi>10.1016/j.febslet.2004.08.044</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| source | Wiley Free Content; MEDLINE; Wiley Online Library Journals Frontfile Complete; Elsevier ScienceDirect Journals; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Aged Animals Cell Line Chloride channel Chloride Channels - genetics Chloride Channels - metabolism Chlorides - metabolism ClC-2 Female Gastric acid Gastric Acid - secretion Gastric Mucosa - cytology Gastric Mucosa - metabolism H(+)-K(+)-Exchanging ATPase - genetics H(+)-K(+)-Exchanging ATPase - metabolism Humans Male Middle Aged Parietal Cells, Gastric - cytology Parietal Cells, Gastric - secretion Rabbits Rats RNA, Messenger - metabolism Stomach - anatomy & histology Stomach - metabolism |
| title | Is the ClC-2 chloride channel involved in the Cl − secretory mechanism of gastric parietal cells? |
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