Use of two real-time polymerase chain reactions (PCRs) to detect herpes simplex type 1 and 2-DNA after automated extraction of nucleic acid

Herpes simplex virus infections may be diagnosed by several techniques, including conventional cell culture and the polymerase chain reaction (PCR). This prospective study compares the analytical performances and usefulness of an in‐house real‐time PCR method and the Light Cycler HSV ½ detection kit...

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Veröffentlicht in:	Journal of medical virology 2004-11, Vol.74 (3), p.459-462
Hauptverfasser:	Mengelle, C., Sandres-Sauné, K., Miédougé, M., Mansuy, J.-M., Bouquies, C., Izopet, J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Biological and medical sciences Cytopathogenic Effect, Viral DNA Primers - genetics DNA, Viral - genetics DNA, Viral - isolation & purification Fundamental and applied biological sciences. Psychology Herpes Simplex - diagnosis Herpes Simplex - virology Herpesvirus 1, Human - genetics Herpesvirus 1, Human - isolation & purification Herpesvirus 2, Human - genetics Herpesvirus 2, Human - isolation & purification HSV infection Human viral diseases Humans Infectious diseases laboratory diagnosis Light Cycler Medical sciences Microbiology Miscellaneous Polymerase Chain Reaction - methods Polymerase Chain Reaction - statistics & numerical data Predictive Value of Tests Prospective Studies Sensitivity and Specificity Viral diseases Viral diseases with cutaneous or mucosal lesions and viral diseases of the eye Virology
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