Helicobacter pylori DNA in dental plaques, gastroscopy, and dental devices
The role of dental plaque in the transmission of Helicobacter pylori (Hp) is unclear due to variability in the detection rates and techniques used. We used nested PCR to estimate the incidence of Hp in dental plaques of 24 dental hygienists. We found an unexpectedly high incidence (50%) of Hp DNA in...
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| Veröffentlicht in: | Digestive diseases and sciences 2004-08, Vol.49 (7-8), p.1091-1094 |
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| creator | ABD AL NASSER AL-HAWAJRI KERET, Dan SIMHON, Albert ZLOTKIN, Amir FISHMAN, Yolanta BERCOVIER, Herve RAHAV, Galia |
| description | The role of dental plaque in the transmission of Helicobacter pylori (Hp) is unclear due to variability in the detection rates and techniques used. We used nested PCR to estimate the incidence of Hp in dental plaques of 24 dental hygienists. We found an unexpectedly high incidence (50%) of Hp DNA in dental plaques using sterilized dental probes. Additional treatment of sonication and SDS wash prior to sterilization of dental probes reduced the incidence to 13%. We used the treated probes to assess Hp presence in plaque samples of 47 patients visiting the dental clinic for teeth cleaning. Hp DNA was detected in 24% of cases. Since these data may reflect instrument contamination, we tested dental probes, endoscopes, and endoscopy forceps and found that 12.5-37.5% of them were contaminated. Consequently, dental plaques may be a candidate reservoir for Hp, medical equipment may contribute to Hp transmission, and sample collection techniques can bias the true prevalence of Hp in a population. |
| doi_str_mv | 10.1023/B:DDAS.0000037793.28069.44 |
| format | Article |
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We used nested PCR to estimate the incidence of Hp in dental plaques of 24 dental hygienists. We found an unexpectedly high incidence (50%) of Hp DNA in dental plaques using sterilized dental probes. Additional treatment of sonication and SDS wash prior to sterilization of dental probes reduced the incidence to 13%. We used the treated probes to assess Hp presence in plaque samples of 47 patients visiting the dental clinic for teeth cleaning. Hp DNA was detected in 24% of cases. Since these data may reflect instrument contamination, we tested dental probes, endoscopes, and endoscopy forceps and found that 12.5-37.5% of them were contaminated. Consequently, dental plaques may be a candidate reservoir for Hp, medical equipment may contribute to Hp transmission, and sample collection techniques can bias the true prevalence of Hp in a population.</description><identifier>ISSN: 0163-2116</identifier><identifier>EISSN: 1573-2568</identifier><identifier>DOI: 10.1023/B:DDAS.0000037793.28069.44</identifier><identifier>PMID: 15387327</identifier><identifier>CODEN: DDSCDJ</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>Adult ; Bacterial diseases ; Bacterial diseases of the digestive system and abdomen ; Biological and medical sciences ; Dental Equipment - microbiology ; Dental Plaque - microbiology ; Disinfection ; DNA, Bacterial - isolation & purification ; Equipment Contamination ; Feeding. Feeding behavior ; Female ; Fundamental and applied biological sciences. Psychology ; Gastroscopes - microbiology ; Helicobacter Infections - transmission ; Helicobacter pylori ; Helicobacter pylori - isolation & purification ; Human bacterial diseases ; Humans ; Infectious diseases ; Male ; Medical sciences ; Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><ispartof>Digestive diseases and sciences, 2004-08, Vol.49 (7-8), p.1091-1094</ispartof><rights>2004 INIST-CNRS</rights><rights>Springer Science+Business Media, Inc. 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c403t-5e356083fa3f760fa0efb65f5c09e67f99854dd99cce4d504d6d5610d928a8d73</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,27933,27934</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16121369$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15387327$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>ABD AL NASSER AL-HAWAJRI</creatorcontrib><creatorcontrib>KERET, Dan</creatorcontrib><creatorcontrib>SIMHON, Albert</creatorcontrib><creatorcontrib>ZLOTKIN, Amir</creatorcontrib><creatorcontrib>FISHMAN, Yolanta</creatorcontrib><creatorcontrib>BERCOVIER, Herve</creatorcontrib><creatorcontrib>RAHAV, Galia</creatorcontrib><title>Helicobacter pylori DNA in dental plaques, gastroscopy, and dental devices</title><title>Digestive diseases and sciences</title><addtitle>Dig Dis Sci</addtitle><description>The role of dental plaque in the transmission of Helicobacter pylori (Hp) is unclear due to variability in the detection rates and techniques used. We used nested PCR to estimate the incidence of Hp in dental plaques of 24 dental hygienists. We found an unexpectedly high incidence (50%) of Hp DNA in dental plaques using sterilized dental probes. Additional treatment of sonication and SDS wash prior to sterilization of dental probes reduced the incidence to 13%. We used the treated probes to assess Hp presence in plaque samples of 47 patients visiting the dental clinic for teeth cleaning. Hp DNA was detected in 24% of cases. Since these data may reflect instrument contamination, we tested dental probes, endoscopes, and endoscopy forceps and found that 12.5-37.5% of them were contaminated. 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Psychology</subject><subject>Gastroscopes - microbiology</subject><subject>Helicobacter Infections - transmission</subject><subject>Helicobacter pylori</subject><subject>Helicobacter pylori - isolation & purification</subject><subject>Human bacterial diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><issn>0163-2116</issn><issn>1573-2568</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNqF0UlLAzEUB_AgitblK8hQ0FNbk8neWxdXRA_qOaRZZGQ6MyYzQr-9qa0UvJhLcvi9l-T9AegjOEIwx1fT8Xw-eRnB9cKcSzzKBWRyRMge6CHK8TCnTOyDHkQsnRFiR-A4xo_EJUfsEBwhigXHOe-BhztXFqZeaNO6kDWrsg5FNn-aZEWVWVe1usyaUn92Lg6ydx3bUEdTN6tBpiv7C6z7KoyLp-DA6zK6s-1-At5url9nd8PH59v72eRxaAjE7ZA6TBkU2GvsOYNeQ-cXjHpqoHSMeykFJdZKaYwjlkJimaUMQStzoYXl-ARcbvo2oV4_rFXLIhpXlrpydRcVYxKmgdB_IUodOZYwwf4f-FF3oUqfUDkiGAshUELjDTJpBjE4r5pQLHVYKQTVOhc1Vetc1C4X9ZOLIiQVn29v6BZLZ3el2yASuNgCHY0ufdCVKeLOMZQjzCT-Bq7HlN0</recordid><startdate>20040801</startdate><enddate>20040801</enddate><creator>ABD AL NASSER AL-HAWAJRI</creator><creator>KERET, Dan</creator><creator>SIMHON, Albert</creator><creator>ZLOTKIN, Amir</creator><creator>FISHMAN, Yolanta</creator><creator>BERCOVIER, Herve</creator><creator>RAHAV, Galia</creator><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>M0R</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7QL</scope><scope>7TM</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20040801</creationdate><title>Helicobacter pylori DNA in dental plaques, gastroscopy, and dental devices</title><author>ABD AL NASSER AL-HAWAJRI ; KERET, Dan ; SIMHON, Albert ; ZLOTKIN, Amir ; FISHMAN, Yolanta ; BERCOVIER, Herve ; RAHAV, Galia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c403t-5e356083fa3f760fa0efb65f5c09e67f99854dd99cce4d504d6d5610d928a8d73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Adult</topic><topic>Bacterial diseases</topic><topic>Bacterial diseases of the digestive system and abdomen</topic><topic>Biological and medical sciences</topic><topic>Dental Equipment - microbiology</topic><topic>Dental Plaque - microbiology</topic><topic>Disinfection</topic><topic>DNA, Bacterial - isolation & purification</topic><topic>Equipment Contamination</topic><topic>Feeding. Feeding behavior</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gastroscopes - microbiology</topic><topic>Helicobacter Infections - transmission</topic><topic>Helicobacter pylori</topic><topic>Helicobacter pylori - isolation & purification</topic><topic>Human bacterial diseases</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Vertebrates: anatomy and physiology, studies on body, several organs or systems</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ABD AL NASSER AL-HAWAJRI</creatorcontrib><creatorcontrib>KERET, Dan</creatorcontrib><creatorcontrib>SIMHON, Albert</creatorcontrib><creatorcontrib>ZLOTKIN, Amir</creatorcontrib><creatorcontrib>FISHMAN, Yolanta</creatorcontrib><creatorcontrib>BERCOVIER, Herve</creatorcontrib><creatorcontrib>RAHAV, Galia</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Digestive diseases and sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ABD AL NASSER AL-HAWAJRI</au><au>KERET, Dan</au><au>SIMHON, Albert</au><au>ZLOTKIN, Amir</au><au>FISHMAN, Yolanta</au><au>BERCOVIER, Herve</au><au>RAHAV, Galia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Helicobacter pylori DNA in dental plaques, gastroscopy, and dental devices</atitle><jtitle>Digestive diseases and sciences</jtitle><addtitle>Dig Dis Sci</addtitle><date>2004-08-01</date><risdate>2004</risdate><volume>49</volume><issue>7-8</issue><spage>1091</spage><epage>1094</epage><pages>1091-1094</pages><issn>0163-2116</issn><eissn>1573-2568</eissn><coden>DDSCDJ</coden><abstract>The role of dental plaque in the transmission of Helicobacter pylori (Hp) is unclear due to variability in the detection rates and techniques used. We used nested PCR to estimate the incidence of Hp in dental plaques of 24 dental hygienists. We found an unexpectedly high incidence (50%) of Hp DNA in dental plaques using sterilized dental probes. Additional treatment of sonication and SDS wash prior to sterilization of dental probes reduced the incidence to 13%. We used the treated probes to assess Hp presence in plaque samples of 47 patients visiting the dental clinic for teeth cleaning. Hp DNA was detected in 24% of cases. Since these data may reflect instrument contamination, we tested dental probes, endoscopes, and endoscopy forceps and found that 12.5-37.5% of them were contaminated. Consequently, dental plaques may be a candidate reservoir for Hp, medical equipment may contribute to Hp transmission, and sample collection techniques can bias the true prevalence of Hp in a population.</abstract><cop>Heidelberg</cop><pub>Springer</pub><pmid>15387327</pmid><doi>10.1023/B:DDAS.0000037793.28069.44</doi><tpages>4</tpages></addata></record> |
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| ispartof | Digestive diseases and sciences, 2004-08, Vol.49 (7-8), p.1091-1094 |
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| subjects | Adult Bacterial diseases Bacterial diseases of the digestive system and abdomen Biological and medical sciences Dental Equipment - microbiology Dental Plaque - microbiology Disinfection DNA, Bacterial - isolation & purification Equipment Contamination Feeding. Feeding behavior Female Fundamental and applied biological sciences. Psychology Gastroscopes - microbiology Helicobacter Infections - transmission Helicobacter pylori Helicobacter pylori - isolation & purification Human bacterial diseases Humans Infectious diseases Male Medical sciences Vertebrates: anatomy and physiology, studies on body, several organs or systems |
| title | Helicobacter pylori DNA in dental plaques, gastroscopy, and dental devices |
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