New planar substrates for the in situ synthesis of peptide arrays
The performance of new cellulose membranes, aminofunctionalized via a PEG spacer, as a solid support in the synthesis of peptide arrays is described. The new membranes are stable to trifluoroacetic acid (TFA) and strong aqueous base for days. These properties extend the scope of synthesis considerab...
Gespeichert in:
| Veröffentlicht in: | Molecular diversity 2004, Vol.8 (3), p.189-195 |
|---|---|
| 1. Verfasser: | |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 195 |
|---|---|
| container_issue | 3 |
| container_start_page | 189 |
| container_title | Molecular diversity |
| container_volume | 8 |
| creator | Zander, Norbert |
| description | The performance of new cellulose membranes, aminofunctionalized via a PEG spacer, as a solid support in the synthesis of peptide arrays is described. The new membranes are stable to trifluoroacetic acid (TFA) and strong aqueous base for days. These properties extend the scope of synthesis considerably, e.g., more efficient side chain cleavage protocols can be applied which yielded peptides of improved purity. For the first time, cellulose membranes with a loading as high as 5 micromol/cm2 were accessible. Additionally, newly developed polypropylene membranes with hydroxy- or amino functionalities were successfully employed for the SPOT synthesis of peptides and phosphopeptides. The membranes are compatible with antibody binding as well as enzymatic phosphorylation assays. |
| doi_str_mv | 10.1023/B:MODI.0000036253.04738.ac |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_66901917</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2143100121</sourcerecordid><originalsourceid>FETCH-LOGICAL-c342t-820cf5122d39629b18b3e448098c840b066dcf89891cf505a961874670b746be3</originalsourceid><addsrcrecordid>eNpdkN9LwzAQgIMobk7_BQl78K01l6Rpsrdt_hpM96LgW0jbFDu2tiYtsv_ezA0GHuFyge9yx4fQGEgMhLL72eR19bCIyT6YoAmLCU-ZjE1-hoaQpCxKCHyeh5pJiEApGKAr79eEQDjsEg0gYZJzoEM0fbM_uN2Y2jjs-8x3znTW47JxuPuyuKqxr7oe-10dnr7yuClxa9uuKiw2zpmdv0YXpdl4e3O8R-jj6fF9_hItV8-L-XQZ5YzTLpKU5GUClBZMCaoykBmznEuiZC45yYgQRV5KJRUEjiRGCZApFynJQs4sG6G7w7-ta7576zu9rXxuN2F32_ReC6EIKEgDOP4Hrpve1WE3nSagaEoUD9DkAOWu8d7ZUreu2hq300D03rKe6b1lfbKs_yxrk4fm2-OEPtva4tR61Mp-AUjZd4c</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>751927094</pqid></control><display><type>article</type><title>New planar substrates for the in situ synthesis of peptide arrays</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Zander, Norbert</creator><creatorcontrib>Zander, Norbert</creatorcontrib><description>The performance of new cellulose membranes, aminofunctionalized via a PEG spacer, as a solid support in the synthesis of peptide arrays is described. The new membranes are stable to trifluoroacetic acid (TFA) and strong aqueous base for days. These properties extend the scope of synthesis considerably, e.g., more efficient side chain cleavage protocols can be applied which yielded peptides of improved purity. For the first time, cellulose membranes with a loading as high as 5 micromol/cm2 were accessible. Additionally, newly developed polypropylene membranes with hydroxy- or amino functionalities were successfully employed for the SPOT synthesis of peptides and phosphopeptides. The membranes are compatible with antibody binding as well as enzymatic phosphorylation assays.</description><identifier>ISSN: 1381-1991</identifier><identifier>EISSN: 1573-501X</identifier><identifier>DOI: 10.1023/B:MODI.0000036253.04738.ac</identifier><identifier>PMID: 15384412</identifier><language>eng</language><publisher>Netherlands: Springer Nature B.V</publisher><subject>Indicators and Reagents ; Microscopy, Fluorescence ; Oligonucleotides - chemical synthesis ; Peptides ; Peptides - chemical synthesis ; Peptides - chemistry ; Protein Array Analysis ; Proteins - chemical synthesis</subject><ispartof>Molecular diversity, 2004, Vol.8 (3), p.189-195</ispartof><rights>Kluwer Academic Publishers 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c342t-820cf5122d39629b18b3e448098c840b066dcf89891cf505a961874670b746be3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15384412$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zander, Norbert</creatorcontrib><title>New planar substrates for the in situ synthesis of peptide arrays</title><title>Molecular diversity</title><addtitle>Mol Divers</addtitle><description>The performance of new cellulose membranes, aminofunctionalized via a PEG spacer, as a solid support in the synthesis of peptide arrays is described. The new membranes are stable to trifluoroacetic acid (TFA) and strong aqueous base for days. These properties extend the scope of synthesis considerably, e.g., more efficient side chain cleavage protocols can be applied which yielded peptides of improved purity. For the first time, cellulose membranes with a loading as high as 5 micromol/cm2 were accessible. Additionally, newly developed polypropylene membranes with hydroxy- or amino functionalities were successfully employed for the SPOT synthesis of peptides and phosphopeptides. The membranes are compatible with antibody binding as well as enzymatic phosphorylation assays.</description><subject>Indicators and Reagents</subject><subject>Microscopy, Fluorescence</subject><subject>Oligonucleotides - chemical synthesis</subject><subject>Peptides</subject><subject>Peptides - chemical synthesis</subject><subject>Peptides - chemistry</subject><subject>Protein Array Analysis</subject><subject>Proteins - chemical synthesis</subject><issn>1381-1991</issn><issn>1573-501X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkN9LwzAQgIMobk7_BQl78K01l6Rpsrdt_hpM96LgW0jbFDu2tiYtsv_ezA0GHuFyge9yx4fQGEgMhLL72eR19bCIyT6YoAmLCU-ZjE1-hoaQpCxKCHyeh5pJiEApGKAr79eEQDjsEg0gYZJzoEM0fbM_uN2Y2jjs-8x3znTW47JxuPuyuKqxr7oe-10dnr7yuClxa9uuKiw2zpmdv0YXpdl4e3O8R-jj6fF9_hItV8-L-XQZ5YzTLpKU5GUClBZMCaoykBmznEuiZC45yYgQRV5KJRUEjiRGCZApFynJQs4sG6G7w7-ta7576zu9rXxuN2F32_ReC6EIKEgDOP4Hrpve1WE3nSagaEoUD9DkAOWu8d7ZUreu2hq300D03rKe6b1lfbKs_yxrk4fm2-OEPtva4tR61Mp-AUjZd4c</recordid><startdate>2004</startdate><enddate>2004</enddate><creator>Zander, Norbert</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>2004</creationdate><title>New planar substrates for the in situ synthesis of peptide arrays</title><author>Zander, Norbert</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c342t-820cf5122d39629b18b3e448098c840b066dcf89891cf505a961874670b746be3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Indicators and Reagents</topic><topic>Microscopy, Fluorescence</topic><topic>Oligonucleotides - chemical synthesis</topic><topic>Peptides</topic><topic>Peptides - chemical synthesis</topic><topic>Peptides - chemistry</topic><topic>Protein Array Analysis</topic><topic>Proteins - chemical synthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zander, Norbert</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular diversity</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zander, Norbert</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>New planar substrates for the in situ synthesis of peptide arrays</atitle><jtitle>Molecular diversity</jtitle><addtitle>Mol Divers</addtitle><date>2004</date><risdate>2004</risdate><volume>8</volume><issue>3</issue><spage>189</spage><epage>195</epage><pages>189-195</pages><issn>1381-1991</issn><eissn>1573-501X</eissn><abstract>The performance of new cellulose membranes, aminofunctionalized via a PEG spacer, as a solid support in the synthesis of peptide arrays is described. The new membranes are stable to trifluoroacetic acid (TFA) and strong aqueous base for days. These properties extend the scope of synthesis considerably, e.g., more efficient side chain cleavage protocols can be applied which yielded peptides of improved purity. For the first time, cellulose membranes with a loading as high as 5 micromol/cm2 were accessible. Additionally, newly developed polypropylene membranes with hydroxy- or amino functionalities were successfully employed for the SPOT synthesis of peptides and phosphopeptides. The membranes are compatible with antibody binding as well as enzymatic phosphorylation assays.</abstract><cop>Netherlands</cop><pub>Springer Nature B.V</pub><pmid>15384412</pmid><doi>10.1023/B:MODI.0000036253.04738.ac</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1381-1991 |
| ispartof | Molecular diversity, 2004, Vol.8 (3), p.189-195 |
| issn | 1381-1991 1573-501X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_66901917 |
| source | MEDLINE; SpringerLink Journals |
| subjects | Indicators and Reagents Microscopy, Fluorescence Oligonucleotides - chemical synthesis Peptides Peptides - chemical synthesis Peptides - chemistry Protein Array Analysis Proteins - chemical synthesis |
| title | New planar substrates for the in situ synthesis of peptide arrays |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T10%3A05%3A16IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=New%20planar%20substrates%20for%20the%20in%20situ%20synthesis%20of%20peptide%20arrays&rft.jtitle=Molecular%20diversity&rft.au=Zander,%20Norbert&rft.date=2004&rft.volume=8&rft.issue=3&rft.spage=189&rft.epage=195&rft.pages=189-195&rft.issn=1381-1991&rft.eissn=1573-501X&rft_id=info:doi/10.1023/B:MODI.0000036253.04738.ac&rft_dat=%3Cproquest_cross%3E2143100121%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=751927094&rft_id=info:pmid/15384412&rfr_iscdi=true |