Improved transfection of spleen-derived antigen-presenting cells in culture using TATp-liposomes
Antigen presenting cells (APC) are among the most important cells of the immune system since they link the innate and the adaptative immune responses, directing the type of immune response to be elicited. To modulate the immune response in immune preventing or treating therapies, gene delivery into...
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| Veröffentlicht in: | Journal of controlled release 2009-02, Vol.134 (1), p.41-46 |
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| creator | Pappalardo, Juan Sebastián Quattrocchi, Valeria Langellotti, Cecilia Di Giacomo, Sebastián Gnazzo, Victoria Olivera, Valeria Calamante, Gabriela Zamorano, Patricia I. Levchenko, Tatyana S. Torchilin, Vladimir P. |
| description | Antigen presenting cells (APC) are among the most important cells of the immune system since they link the innate and the adaptative immune responses, directing the type of immune response to be elicited. To modulate the immune response in immune preventing or treating therapies, gene delivery into immunocompetent cells could be used. However, APC are very resistant to transfection. To increase the efficiency of APC transfection, we have used liposome-based lipoplexes additionally modified with cell-penetrating TAT peptide (TATp) for better intracellular delivery of a model plasmid encoding for the enhanced-green fluorescent protein (pEGFP). pEGFP-bearing lipoplexes made of a mixture of PC:Chol:DOTAP (60:30:10 molar ratio) with the addition of 2% mol of polyethylene glycol-phosphatidylethanolamine (PEG-PE) conjugate (plain-L) or TATp-PEG-PE (TATp-L) were shown to effectively protect the incorporated DNA from degradation. Uptake assays of rhodamine-labeled lipoplexes and transfections with the EGFP reporter gene were performed with APC derived from the mouse spleen. TATp-L-based lipoplexes allowed for significantly enhanced both, the uptake and transfection in APC. Such a tool could be used for the APC transfection as a first step in immune therapy. |
| doi_str_mv | 10.1016/j.jconrel.2008.11.006 |
| format | Article |
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To modulate the immune response in immune preventing or treating therapies, gene delivery into immunocompetent cells could be used. However, APC are very resistant to transfection. To increase the efficiency of APC transfection, we have used liposome-based lipoplexes additionally modified with cell-penetrating TAT peptide (TATp) for better intracellular delivery of a model plasmid encoding for the enhanced-green fluorescent protein (pEGFP). pEGFP-bearing lipoplexes made of a mixture of PC:Chol:DOTAP (60:30:10 molar ratio) with the addition of 2% mol of polyethylene glycol-phosphatidylethanolamine (PEG-PE) conjugate (plain-L) or TATp-PEG-PE (TATp-L) were shown to effectively protect the incorporated DNA from degradation. Uptake assays of rhodamine-labeled lipoplexes and transfections with the EGFP reporter gene were performed with APC derived from the mouse spleen. TATp-L-based lipoplexes allowed for significantly enhanced both, the uptake and transfection in APC. Such a tool could be used for the APC transfection as a first step in immune therapy.</description><identifier>ISSN: 0168-3659</identifier><identifier>EISSN: 1873-4995</identifier><identifier>DOI: 10.1016/j.jconrel.2008.11.006</identifier><identifier>PMID: 19059290</identifier><identifier>CODEN: JCREEC</identifier><language>eng</language><publisher>Kidlington: Elsevier B.V</publisher><subject>Animals ; Antigen-presenting cells ; Antigen-Presenting Cells - cytology ; Antigen-Presenting Cells - metabolism ; Biological and medical sciences ; Cell culture ; Cells, Cultured ; DNA - chemistry ; Gene Products, tat - chemistry ; Gene Products, tat - genetics ; General pharmacology ; Green fluorescent protein ; Liposomes ; Liposomes - analysis ; Liposomes - chemistry ; Medical sciences ; Mice ; Pharmaceutical technology. Pharmaceutical industry ; Pharmacology. Drug treatments ; Spleen ; Spleen - cytology ; Spleen - metabolism ; TAT peptide ; Transfection ; Transfection - methods</subject><ispartof>Journal of controlled release, 2009-02, Vol.134 (1), p.41-46</ispartof><rights>2008 Elsevier B.V.</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c424t-419c6c0750401890017de9adefdadb61d9d2e0bce5dddc809a3cddbc2999cc73</citedby><cites>FETCH-LOGICAL-c424t-419c6c0750401890017de9adefdadb61d9d2e0bce5dddc809a3cddbc2999cc73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0168365908007074$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21244798$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19059290$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pappalardo, Juan Sebastián</creatorcontrib><creatorcontrib>Quattrocchi, Valeria</creatorcontrib><creatorcontrib>Langellotti, Cecilia</creatorcontrib><creatorcontrib>Di Giacomo, Sebastián</creatorcontrib><creatorcontrib>Gnazzo, Victoria</creatorcontrib><creatorcontrib>Olivera, Valeria</creatorcontrib><creatorcontrib>Calamante, Gabriela</creatorcontrib><creatorcontrib>Zamorano, Patricia I.</creatorcontrib><creatorcontrib>Levchenko, Tatyana S.</creatorcontrib><creatorcontrib>Torchilin, Vladimir P.</creatorcontrib><title>Improved transfection of spleen-derived antigen-presenting cells in culture using TATp-liposomes</title><title>Journal of controlled release</title><addtitle>J Control Release</addtitle><description>Antigen presenting cells (APC) are among the most important cells of the immune system since they link the innate and the adaptative immune responses, directing the type of immune response to be elicited. To modulate the immune response in immune preventing or treating therapies, gene delivery into immunocompetent cells could be used. However, APC are very resistant to transfection. To increase the efficiency of APC transfection, we have used liposome-based lipoplexes additionally modified with cell-penetrating TAT peptide (TATp) for better intracellular delivery of a model plasmid encoding for the enhanced-green fluorescent protein (pEGFP). pEGFP-bearing lipoplexes made of a mixture of PC:Chol:DOTAP (60:30:10 molar ratio) with the addition of 2% mol of polyethylene glycol-phosphatidylethanolamine (PEG-PE) conjugate (plain-L) or TATp-PEG-PE (TATp-L) were shown to effectively protect the incorporated DNA from degradation. Uptake assays of rhodamine-labeled lipoplexes and transfections with the EGFP reporter gene were performed with APC derived from the mouse spleen. TATp-L-based lipoplexes allowed for significantly enhanced both, the uptake and transfection in APC. Such a tool could be used for the APC transfection as a first step in immune therapy.</description><subject>Animals</subject><subject>Antigen-presenting cells</subject><subject>Antigen-Presenting Cells - cytology</subject><subject>Antigen-Presenting Cells - metabolism</subject><subject>Biological and medical sciences</subject><subject>Cell culture</subject><subject>Cells, Cultured</subject><subject>DNA - chemistry</subject><subject>Gene Products, tat - chemistry</subject><subject>Gene Products, tat - genetics</subject><subject>General pharmacology</subject><subject>Green fluorescent protein</subject><subject>Liposomes</subject><subject>Liposomes - analysis</subject><subject>Liposomes - chemistry</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Pharmaceutical technology. Pharmaceutical industry</subject><subject>Pharmacology. Drug treatments</subject><subject>Spleen</subject><subject>Spleen - cytology</subject><subject>Spleen - metabolism</subject><subject>TAT peptide</subject><subject>Transfection</subject><subject>Transfection - methods</subject><issn>0168-3659</issn><issn>1873-4995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUGP1SAUhYnROG9Gf4KmG921XlqgsDKTiTqTTOLm7ZEHtxNeWqjQTuK_l-Y1upwVcPkunHsOIR8oNBSo-HJuzjaGhGPTAsiG0gZAvCIHKvuuZkrx1-RQOFl3gqsrcp3zGQB4x_q35Ioq4KpVcCC_HqY5xWd01ZJMyAPaxcdQxaHK84gYaofJb9cmLP6pnOeEGcs-PFUWxzFXPlR2HZc1YbXmrXy8Pc716OeY44T5HXkzmDHj-329Icfv34539_Xjzx8Pd7ePtWUtW2pGlRUWeg4MqFQAtHeojMPBGXcS1CnXIpwscueclaBMZ5072VYpZW3f3ZDPl2fLNL9XzIuefN4EmoBxzVoIqSQX_EWwhU4KJmUB-QW0KeaccNBz8pNJfzQFvUWgz3qPQG8RaEp1iaD0fdw_WE8Tuv9du-cF-LQDJlszDsV36_M_rqUtY73aBHy9cFhse_aYdLYeg0XnU4lJu-hfkPIXxxSp8Q</recordid><startdate>20090220</startdate><enddate>20090220</enddate><creator>Pappalardo, Juan Sebastián</creator><creator>Quattrocchi, Valeria</creator><creator>Langellotti, Cecilia</creator><creator>Di Giacomo, Sebastián</creator><creator>Gnazzo, Victoria</creator><creator>Olivera, Valeria</creator><creator>Calamante, Gabriela</creator><creator>Zamorano, Patricia I.</creator><creator>Levchenko, Tatyana S.</creator><creator>Torchilin, Vladimir P.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20090220</creationdate><title>Improved transfection of spleen-derived antigen-presenting cells in culture using TATp-liposomes</title><author>Pappalardo, Juan Sebastián ; Quattrocchi, Valeria ; Langellotti, Cecilia ; Di Giacomo, Sebastián ; Gnazzo, Victoria ; Olivera, Valeria ; Calamante, Gabriela ; Zamorano, Patricia I. ; Levchenko, Tatyana S. ; Torchilin, Vladimir P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c424t-419c6c0750401890017de9adefdadb61d9d2e0bce5dddc809a3cddbc2999cc73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Antigen-presenting cells</topic><topic>Antigen-Presenting Cells - cytology</topic><topic>Antigen-Presenting Cells - metabolism</topic><topic>Biological and medical sciences</topic><topic>Cell culture</topic><topic>Cells, Cultured</topic><topic>DNA - chemistry</topic><topic>Gene Products, tat - chemistry</topic><topic>Gene Products, tat - genetics</topic><topic>General pharmacology</topic><topic>Green fluorescent protein</topic><topic>Liposomes</topic><topic>Liposomes - analysis</topic><topic>Liposomes - chemistry</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Pharmaceutical technology. Pharmaceutical industry</topic><topic>Pharmacology. Drug treatments</topic><topic>Spleen</topic><topic>Spleen - cytology</topic><topic>Spleen - metabolism</topic><topic>TAT peptide</topic><topic>Transfection</topic><topic>Transfection - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pappalardo, Juan Sebastián</creatorcontrib><creatorcontrib>Quattrocchi, Valeria</creatorcontrib><creatorcontrib>Langellotti, Cecilia</creatorcontrib><creatorcontrib>Di Giacomo, Sebastián</creatorcontrib><creatorcontrib>Gnazzo, Victoria</creatorcontrib><creatorcontrib>Olivera, Valeria</creatorcontrib><creatorcontrib>Calamante, Gabriela</creatorcontrib><creatorcontrib>Zamorano, Patricia I.</creatorcontrib><creatorcontrib>Levchenko, Tatyana S.</creatorcontrib><creatorcontrib>Torchilin, Vladimir P.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of controlled release</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pappalardo, Juan Sebastián</au><au>Quattrocchi, Valeria</au><au>Langellotti, Cecilia</au><au>Di Giacomo, Sebastián</au><au>Gnazzo, Victoria</au><au>Olivera, Valeria</au><au>Calamante, Gabriela</au><au>Zamorano, Patricia I.</au><au>Levchenko, Tatyana S.</au><au>Torchilin, Vladimir P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Improved transfection of spleen-derived antigen-presenting cells in culture using TATp-liposomes</atitle><jtitle>Journal of controlled release</jtitle><addtitle>J Control Release</addtitle><date>2009-02-20</date><risdate>2009</risdate><volume>134</volume><issue>1</issue><spage>41</spage><epage>46</epage><pages>41-46</pages><issn>0168-3659</issn><eissn>1873-4995</eissn><coden>JCREEC</coden><abstract>Antigen presenting cells (APC) are among the most important cells of the immune system since they link the innate and the adaptative immune responses, directing the type of immune response to be elicited. 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| ispartof | Journal of controlled release, 2009-02, Vol.134 (1), p.41-46 |
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| subjects | Animals Antigen-presenting cells Antigen-Presenting Cells - cytology Antigen-Presenting Cells - metabolism Biological and medical sciences Cell culture Cells, Cultured DNA - chemistry Gene Products, tat - chemistry Gene Products, tat - genetics General pharmacology Green fluorescent protein Liposomes Liposomes - analysis Liposomes - chemistry Medical sciences Mice Pharmaceutical technology. Pharmaceutical industry Pharmacology. Drug treatments Spleen Spleen - cytology Spleen - metabolism TAT peptide Transfection Transfection - methods |
| title | Improved transfection of spleen-derived antigen-presenting cells in culture using TATp-liposomes |
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