Evaluation of chromogenic media for the isolation of vancomycin-resistant enterococci from stool samples
This study sought to evaluate the performance of two chromogenic media designed for the isolation of vancomycin-resistant enterococci (VRE) and compare them with a traditional bile-esculin medium for the isolation of VRE from stool samples. A total of 285 stool samples were inoculated onto Chromogen...
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| Veröffentlicht in: | Letters in applied microbiology 2009-02, Vol.48 (2), p.230-233 |
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| creator | Asir, K Wilkinson, K Perry, J.D Reed, R.H Gould, F.K |
| description | This study sought to evaluate the performance of two chromogenic media designed for the isolation of vancomycin-resistant enterococci (VRE) and compare them with a traditional bile-esculin medium for the isolation of VRE from stool samples. A total of 285 stool samples were inoculated onto Chromogenic VRE Agar (AES VRE agar; AES Chemunex), chromID VRE (bioMérieux) and VRE Agar (Oxoid) both directly and also following broth enrichment. In total 18 strains of vancomycin-resistant Enterococcus faecium were recovered, including 17 harbouring the vanA gene and one with vanB. On direct culture, the sensitivity of the three media was 66·7%, 77·8% and 44·4% and after broth enrichment 66·7%, 83·3% and 77·8% using AES VRE Agar, chromID VRE and Oxoid VRE Agar respectively. All three media are useful tools for the isolation of VRE from stool samples. AES VRE Agar and bioMérieux chromID VRE are easier to use than Oxoid VRE Agar due to diffusion of black coloration from the latter. This is the first study to evaluate the performance of AES VRE Agar and the first to compare two media containing synthetic chromogens for the isolation of VRE. |
| doi_str_mv | 10.1111/j.1472-765X.2008.02517.x |
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A total of 285 stool samples were inoculated onto Chromogenic VRE Agar (AES VRE agar; AES Chemunex), chromID VRE (bioMérieux) and VRE Agar (Oxoid) both directly and also following broth enrichment. In total 18 strains of vancomycin-resistant Enterococcus faecium were recovered, including 17 harbouring the vanA gene and one with vanB. On direct culture, the sensitivity of the three media was 66·7%, 77·8% and 44·4% and after broth enrichment 66·7%, 83·3% and 77·8% using AES VRE Agar, chromID VRE and Oxoid VRE Agar respectively. All three media are useful tools for the isolation of VRE from stool samples. AES VRE Agar and bioMérieux chromID VRE are easier to use than Oxoid VRE Agar due to diffusion of black coloration from the latter. This is the first study to evaluate the performance of AES VRE Agar and the first to compare two media containing synthetic chromogens for the isolation of VRE.</description><identifier>ISSN: 0266-8254</identifier><identifier>EISSN: 1472-765X</identifier><identifier>DOI: 10.1111/j.1472-765X.2008.02517.x</identifier><identifier>PMID: 19196440</identifier><identifier>CODEN: LAMIE7</identifier><language>eng</language><publisher>Oxford, UK: Oxford, UK : Blackwell Publishing Ltd</publisher><subject>Biological and medical sciences ; Chromogenic Compounds - metabolism ; chromogenic media ; Colony Count, Microbial - methods ; Culture Media - chemistry ; Culture Media - metabolism ; Enterococcus - isolation & purification ; Enterococcus - metabolism ; Enterococcus faecium ; Feces - microbiology ; Fundamental and applied biological sciences. Psychology ; glycopeptide resistant enterococci ; Gram-Positive Bacterial Infections - microbiology ; Humans ; Microbiology ; Vancomycin Resistance ; vancomycin resistant enterococci</subject><ispartof>Letters in applied microbiology, 2009-02, Vol.48 (2), p.230-233</ispartof><rights>2009 The Authors. Journal compilation © 2009 The Society for Applied Microbiology</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4527-99cb8bc74aaf21bd31fe61cf29178cbc9b750793e1e0d2274349b7131e5e40493</citedby><cites>FETCH-LOGICAL-c4527-99cb8bc74aaf21bd31fe61cf29178cbc9b750793e1e0d2274349b7131e5e40493</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1472-765X.2008.02517.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1472-765X.2008.02517.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21059653$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19196440$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Asir, K</creatorcontrib><creatorcontrib>Wilkinson, K</creatorcontrib><creatorcontrib>Perry, J.D</creatorcontrib><creatorcontrib>Reed, R.H</creatorcontrib><creatorcontrib>Gould, F.K</creatorcontrib><title>Evaluation of chromogenic media for the isolation of vancomycin-resistant enterococci from stool samples</title><title>Letters in applied microbiology</title><addtitle>Lett Appl Microbiol</addtitle><description>This study sought to evaluate the performance of two chromogenic media designed for the isolation of vancomycin-resistant enterococci (VRE) and compare them with a traditional bile-esculin medium for the isolation of VRE from stool samples. A total of 285 stool samples were inoculated onto Chromogenic VRE Agar (AES VRE agar; AES Chemunex), chromID VRE (bioMérieux) and VRE Agar (Oxoid) both directly and also following broth enrichment. In total 18 strains of vancomycin-resistant Enterococcus faecium were recovered, including 17 harbouring the vanA gene and one with vanB. On direct culture, the sensitivity of the three media was 66·7%, 77·8% and 44·4% and after broth enrichment 66·7%, 83·3% and 77·8% using AES VRE Agar, chromID VRE and Oxoid VRE Agar respectively. All three media are useful tools for the isolation of VRE from stool samples. AES VRE Agar and bioMérieux chromID VRE are easier to use than Oxoid VRE Agar due to diffusion of black coloration from the latter. This is the first study to evaluate the performance of AES VRE Agar and the first to compare two media containing synthetic chromogens for the isolation of VRE.</description><subject>Biological and medical sciences</subject><subject>Chromogenic Compounds - metabolism</subject><subject>chromogenic media</subject><subject>Colony Count, Microbial - methods</subject><subject>Culture Media - chemistry</subject><subject>Culture Media - metabolism</subject><subject>Enterococcus - isolation & purification</subject><subject>Enterococcus - metabolism</subject><subject>Enterococcus faecium</subject><subject>Feces - microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>glycopeptide resistant enterococci</subject><subject>Gram-Positive Bacterial Infections - microbiology</subject><subject>Humans</subject><subject>Microbiology</subject><subject>Vancomycin Resistance</subject><subject>vancomycin resistant enterococci</subject><issn>0266-8254</issn><issn>1472-765X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU9vGyEQxVHVqnHTfoWWS3vbLcPCshx6iKL0j-QohzRSb4jFEGOxiwvrJP72ZWvLPTZcQMPvzYzeQwgDqaGcz5samKCVaPmvmhLS1YRyEPXTC7Q4fbxEC0LbtuooZ2foTc4bUkig8jU6AwmyZYws0PrqQYednnwccXTYrFMc4r0dvcGDXXmNXUx4Wlvscwwn7EGPJg5748cq2ezzpMcJ23GyKZpojMeutMF5ijHgrIdtsPkteuV0yPbd8T5Hd1-vfl5-r5Y3335cXiwrwzgVlZSm73ojmNaOQr9qwNkWjKMSRGd6I3vBiZCNBUtWlArWsFKCBiy3jDDZnKNPh77bFH_vbJ7U4LOxIejRxl1WbdtJKrr2vyAlDQNGeQG7A2hSzDlZp7bJDzrtFRA1x6E2anZdza6rOQ71Nw71VKTvjzN2fbHzn_DofwE-HgGdjQ4uFWN9PnEUCJctbwr35cA9-mD3z15ALS-u51fRfzjonY5K36cy4-6WEmgI8I6B5M0fouKwsw</recordid><startdate>200902</startdate><enddate>200902</enddate><creator>Asir, K</creator><creator>Wilkinson, K</creator><creator>Perry, J.D</creator><creator>Reed, R.H</creator><creator>Gould, F.K</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>200902</creationdate><title>Evaluation of chromogenic media for the isolation of vancomycin-resistant enterococci from stool samples</title><author>Asir, K ; Wilkinson, K ; Perry, J.D ; Reed, R.H ; Gould, F.K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4527-99cb8bc74aaf21bd31fe61cf29178cbc9b750793e1e0d2274349b7131e5e40493</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Biological and medical sciences</topic><topic>Chromogenic Compounds - metabolism</topic><topic>chromogenic media</topic><topic>Colony Count, Microbial - methods</topic><topic>Culture Media - chemistry</topic><topic>Culture Media - metabolism</topic><topic>Enterococcus - isolation & purification</topic><topic>Enterococcus - metabolism</topic><topic>Enterococcus faecium</topic><topic>Feces - microbiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>glycopeptide resistant enterococci</topic><topic>Gram-Positive Bacterial Infections - microbiology</topic><topic>Humans</topic><topic>Microbiology</topic><topic>Vancomycin Resistance</topic><topic>vancomycin resistant enterococci</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Asir, K</creatorcontrib><creatorcontrib>Wilkinson, K</creatorcontrib><creatorcontrib>Perry, J.D</creatorcontrib><creatorcontrib>Reed, R.H</creatorcontrib><creatorcontrib>Gould, F.K</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Letters in applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Asir, K</au><au>Wilkinson, K</au><au>Perry, J.D</au><au>Reed, R.H</au><au>Gould, F.K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of chromogenic media for the isolation of vancomycin-resistant enterococci from stool samples</atitle><jtitle>Letters in applied microbiology</jtitle><addtitle>Lett Appl Microbiol</addtitle><date>2009-02</date><risdate>2009</risdate><volume>48</volume><issue>2</issue><spage>230</spage><epage>233</epage><pages>230-233</pages><issn>0266-8254</issn><eissn>1472-765X</eissn><coden>LAMIE7</coden><abstract>This study sought to evaluate the performance of two chromogenic media designed for the isolation of vancomycin-resistant enterococci (VRE) and compare them with a traditional bile-esculin medium for the isolation of VRE from stool samples. A total of 285 stool samples were inoculated onto Chromogenic VRE Agar (AES VRE agar; AES Chemunex), chromID VRE (bioMérieux) and VRE Agar (Oxoid) both directly and also following broth enrichment. In total 18 strains of vancomycin-resistant Enterococcus faecium were recovered, including 17 harbouring the vanA gene and one with vanB. On direct culture, the sensitivity of the three media was 66·7%, 77·8% and 44·4% and after broth enrichment 66·7%, 83·3% and 77·8% using AES VRE Agar, chromID VRE and Oxoid VRE Agar respectively. All three media are useful tools for the isolation of VRE from stool samples. AES VRE Agar and bioMérieux chromID VRE are easier to use than Oxoid VRE Agar due to diffusion of black coloration from the latter. This is the first study to evaluate the performance of AES VRE Agar and the first to compare two media containing synthetic chromogens for the isolation of VRE.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><pmid>19196440</pmid><doi>10.1111/j.1472-765X.2008.02517.x</doi><tpages>4</tpages></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current); Wiley Online Library All Journals; Alma/SFX Local Collection |
| subjects | Biological and medical sciences Chromogenic Compounds - metabolism chromogenic media Colony Count, Microbial - methods Culture Media - chemistry Culture Media - metabolism Enterococcus - isolation & purification Enterococcus - metabolism Enterococcus faecium Feces - microbiology Fundamental and applied biological sciences. Psychology glycopeptide resistant enterococci Gram-Positive Bacterial Infections - microbiology Humans Microbiology Vancomycin Resistance vancomycin resistant enterococci |
| title | Evaluation of chromogenic media for the isolation of vancomycin-resistant enterococci from stool samples |
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