Decreased expression of B7 costimulatory molecules and major histocompatibility complex class-I in human hepatocellular carcinoma
Background and Aim: We analyzed the expression of antigen‐processing and antigen‐presenting molecules in surgically resected fresh samples of human hepatocellular carcinoma (HCC) tissue to elucidate a mechanism of immune escape. We also examined the expression of interleukin (IL)‐10 protein, which...
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| Veröffentlicht in: | Journal of gastroenterology and hepatology 2004-10, Vol.19 (10), p.1121-1127 |
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| creator | FUJIWARA, KEISHI HIGASHI, TOSHIHIRO NOUSO, KAZUHIRO NAKATSUKASA, HARUSHIGE KOBAYASHI, YOSHIYUKI UEMURA, MASAYUKI NAKAMURA, SHIN-ICHIRO SATO, SHUICHIRO HANAFUSA, TADASHI YUMOTO, YASUHIRO NAITO, ICHIRO SHIRATORI, YASUSHI |
| description | Background and Aim: We analyzed the expression of antigen‐processing and antigen‐presenting molecules in surgically resected fresh samples of human hepatocellular carcinoma (HCC) tissue to elucidate a mechanism of immune escape. We also examined the expression of interleukin (IL)‐10 protein, which might act to downregulate expression of antigen‐processing and antigen‐presenting molecules.
Methods: Twenty‐eight HCC samples obtained by surgical resection were analyzed for the expression of β2‐microglobulin, heat‐shock protein (HSP)‐70, human leukocyte antigen (HLA) class‐I, CD80 (B7‐1), CD86 (B7‐2) and IL‐10 by immunostaining.
Results: β2‐Microglobulin and HSP‐70 were preserved in all samples. In contrast, the expression of HLA class‐I molecules was significantly reduced according to lowering in the histological grading of tumor differentiation (P = 0.024). Furthermore, B7‐1 and B7‐2 expression was reduced in tumor cells compared with corresponding areas of liver tissue without malignant involvement irrespective of the histological grading of tumors (21% and 36%, respectively). Although IL‐10 protein was expressed in 54% of HCC, no relationship between the expression of IL‐10 and downregulation of B7‐1, B7‐2, and HLA class‐I was evident.
Conclusion: These findings suggest the potential role of B7 co‐stimulatory molecules and HLA class‐I molecules in facilitating HCC escape from immune surveillance without the involvement of IL‐10. |
| doi_str_mv | 10.1111/j.1440-1746.2004.03467.x |
| format | Article |
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Methods: Twenty‐eight HCC samples obtained by surgical resection were analyzed for the expression of β2‐microglobulin, heat‐shock protein (HSP)‐70, human leukocyte antigen (HLA) class‐I, CD80 (B7‐1), CD86 (B7‐2) and IL‐10 by immunostaining.
Results: β2‐Microglobulin and HSP‐70 were preserved in all samples. In contrast, the expression of HLA class‐I molecules was significantly reduced according to lowering in the histological grading of tumor differentiation (P = 0.024). Furthermore, B7‐1 and B7‐2 expression was reduced in tumor cells compared with corresponding areas of liver tissue without malignant involvement irrespective of the histological grading of tumors (21% and 36%, respectively). Although IL‐10 protein was expressed in 54% of HCC, no relationship between the expression of IL‐10 and downregulation of B7‐1, B7‐2, and HLA class‐I was evident.
Conclusion: These findings suggest the potential role of B7 co‐stimulatory molecules and HLA class‐I molecules in facilitating HCC escape from immune surveillance without the involvement of IL‐10.</description><identifier>ISSN: 0815-9319</identifier><identifier>EISSN: 1440-1746</identifier><identifier>DOI: 10.1111/j.1440-1746.2004.03467.x</identifier><identifier>PMID: 15377288</identifier><language>eng</language><publisher>Melbourne, Australia: Blackwell Science Pty</publisher><subject>Aged ; B7-1 Antigen - biosynthesis ; beta 2-Microglobulin - biosynthesis ; beta 2-Microglobulin - immunology ; Biological and medical sciences ; Carcinoma, Hepatocellular - immunology ; Female ; Gastroenterology. Liver. Pancreas. Abdomen ; Genes, MHC Class I - immunology ; HSP70 Heat-Shock Proteins - biosynthesis ; HSP70 Heat-Shock Proteins - immunology ; Humans ; immune tolerance ; Interleukin-10 - biosynthesis ; Interleukin-10 - immunology ; liver ; Liver Neoplasms - immunology ; Liver. Biliary tract. Portal circulation. Exocrine pancreas ; Male ; Medical sciences ; neoplasm ; tumor escape ; Tumors</subject><ispartof>Journal of gastroenterology and hepatology, 2004-10, Vol.19 (10), p.1121-1127</ispartof><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4997-8510c2f10f7e5054d0ac01e812e9ddd6ff13ff4bda4eb3861b62b5df89c658943</citedby><cites>FETCH-LOGICAL-c4997-8510c2f10f7e5054d0ac01e812e9ddd6ff13ff4bda4eb3861b62b5df89c658943</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1440-1746.2004.03467.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1440-1746.2004.03467.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16164849$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15377288$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>FUJIWARA, KEISHI</creatorcontrib><creatorcontrib>HIGASHI, TOSHIHIRO</creatorcontrib><creatorcontrib>NOUSO, KAZUHIRO</creatorcontrib><creatorcontrib>NAKATSUKASA, HARUSHIGE</creatorcontrib><creatorcontrib>KOBAYASHI, YOSHIYUKI</creatorcontrib><creatorcontrib>UEMURA, MASAYUKI</creatorcontrib><creatorcontrib>NAKAMURA, SHIN-ICHIRO</creatorcontrib><creatorcontrib>SATO, SHUICHIRO</creatorcontrib><creatorcontrib>HANAFUSA, TADASHI</creatorcontrib><creatorcontrib>YUMOTO, YASUHIRO</creatorcontrib><creatorcontrib>NAITO, ICHIRO</creatorcontrib><creatorcontrib>SHIRATORI, YASUSHI</creatorcontrib><title>Decreased expression of B7 costimulatory molecules and major histocompatibility complex class-I in human hepatocellular carcinoma</title><title>Journal of gastroenterology and hepatology</title><addtitle>J Gastroenterol Hepatol</addtitle><description>Background and Aim: We analyzed the expression of antigen‐processing and antigen‐presenting molecules in surgically resected fresh samples of human hepatocellular carcinoma (HCC) tissue to elucidate a mechanism of immune escape. We also examined the expression of interleukin (IL)‐10 protein, which might act to downregulate expression of antigen‐processing and antigen‐presenting molecules.
Methods: Twenty‐eight HCC samples obtained by surgical resection were analyzed for the expression of β2‐microglobulin, heat‐shock protein (HSP)‐70, human leukocyte antigen (HLA) class‐I, CD80 (B7‐1), CD86 (B7‐2) and IL‐10 by immunostaining.
Results: β2‐Microglobulin and HSP‐70 were preserved in all samples. In contrast, the expression of HLA class‐I molecules was significantly reduced according to lowering in the histological grading of tumor differentiation (P = 0.024). Furthermore, B7‐1 and B7‐2 expression was reduced in tumor cells compared with corresponding areas of liver tissue without malignant involvement irrespective of the histological grading of tumors (21% and 36%, respectively). Although IL‐10 protein was expressed in 54% of HCC, no relationship between the expression of IL‐10 and downregulation of B7‐1, B7‐2, and HLA class‐I was evident.
Conclusion: These findings suggest the potential role of B7 co‐stimulatory molecules and HLA class‐I molecules in facilitating HCC escape from immune surveillance without the involvement of IL‐10.</description><subject>Aged</subject><subject>B7-1 Antigen - biosynthesis</subject><subject>beta 2-Microglobulin - biosynthesis</subject><subject>beta 2-Microglobulin - immunology</subject><subject>Biological and medical sciences</subject><subject>Carcinoma, Hepatocellular - immunology</subject><subject>Female</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>Genes, MHC Class I - immunology</subject><subject>HSP70 Heat-Shock Proteins - biosynthesis</subject><subject>HSP70 Heat-Shock Proteins - immunology</subject><subject>Humans</subject><subject>immune tolerance</subject><subject>Interleukin-10 - biosynthesis</subject><subject>Interleukin-10 - immunology</subject><subject>liver</subject><subject>Liver Neoplasms - immunology</subject><subject>Liver. Biliary tract. Portal circulation. Exocrine pancreas</subject><subject>Male</subject><subject>Medical sciences</subject><subject>neoplasm</subject><subject>tumor escape</subject><subject>Tumors</subject><issn>0815-9319</issn><issn>1440-1746</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU-P1CAYh4nRuOPqVzBc9NYKLVB68KCjzq7ZrMasMfFCKH3JMtIyC22cOfrNpc5k9yoH_oTn9_LmASFMSUnzeLMtKWOkoA0TZUUIK0nNRFPuH6HV_cVjtCKS8qKtaXuGnqW0JZkkDX-Kziivm6aScoX-fAATQSfoMex3EVJyYcTB4vcNNiFNbpi9nkI84CF4MLOHhPXY40FvQ8S3Lk3BhGGnJ9c576YDXk4e9th4nVJxid2Ib-dB5xkyFQx4nytGbHQ0bgyDfo6eWO0TvDit5-j7p48364vi6svmcv3uqjCsbZtCckpMZSmxDXDCWU-0IRQkraDt-15YS2trWddrBl0tBe1E1fHeytYILltWn6PXx7q7GO5mSJMaXFra0SOEOSkhZEsZrzIoj6CJIaUIVu2iG3Q8KErUol9t1WJZLZbVol_906_2Ofry9MbcDdA_BE--M_DqBOhktLdRj8alB05QwSRrM_f2yP12Hg7_3YD6vLlYdjlfHPP5g2B_n9fxl8q3DVc_rjeK19_k9df1jfpZ_wVr_LLq</recordid><startdate>200410</startdate><enddate>200410</enddate><creator>FUJIWARA, KEISHI</creator><creator>HIGASHI, TOSHIHIRO</creator><creator>NOUSO, KAZUHIRO</creator><creator>NAKATSUKASA, HARUSHIGE</creator><creator>KOBAYASHI, YOSHIYUKI</creator><creator>UEMURA, MASAYUKI</creator><creator>NAKAMURA, SHIN-ICHIRO</creator><creator>SATO, SHUICHIRO</creator><creator>HANAFUSA, TADASHI</creator><creator>YUMOTO, YASUHIRO</creator><creator>NAITO, ICHIRO</creator><creator>SHIRATORI, YASUSHI</creator><general>Blackwell Science Pty</general><general>Blackwell Science</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200410</creationdate><title>Decreased expression of B7 costimulatory molecules and major histocompatibility complex class-I in human hepatocellular carcinoma</title><author>FUJIWARA, KEISHI ; HIGASHI, TOSHIHIRO ; NOUSO, KAZUHIRO ; NAKATSUKASA, HARUSHIGE ; KOBAYASHI, YOSHIYUKI ; UEMURA, MASAYUKI ; NAKAMURA, SHIN-ICHIRO ; SATO, SHUICHIRO ; HANAFUSA, TADASHI ; YUMOTO, YASUHIRO ; NAITO, ICHIRO ; SHIRATORI, YASUSHI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4997-8510c2f10f7e5054d0ac01e812e9ddd6ff13ff4bda4eb3861b62b5df89c658943</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Aged</topic><topic>B7-1 Antigen - biosynthesis</topic><topic>beta 2-Microglobulin - biosynthesis</topic><topic>beta 2-Microglobulin - immunology</topic><topic>Biological and medical sciences</topic><topic>Carcinoma, Hepatocellular - immunology</topic><topic>Female</topic><topic>Gastroenterology. Liver. Pancreas. Abdomen</topic><topic>Genes, MHC Class I - immunology</topic><topic>HSP70 Heat-Shock Proteins - biosynthesis</topic><topic>HSP70 Heat-Shock Proteins - immunology</topic><topic>Humans</topic><topic>immune tolerance</topic><topic>Interleukin-10 - biosynthesis</topic><topic>Interleukin-10 - immunology</topic><topic>liver</topic><topic>Liver Neoplasms - immunology</topic><topic>Liver. Biliary tract. Portal circulation. Exocrine pancreas</topic><topic>Male</topic><topic>Medical sciences</topic><topic>neoplasm</topic><topic>tumor escape</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>FUJIWARA, KEISHI</creatorcontrib><creatorcontrib>HIGASHI, TOSHIHIRO</creatorcontrib><creatorcontrib>NOUSO, KAZUHIRO</creatorcontrib><creatorcontrib>NAKATSUKASA, HARUSHIGE</creatorcontrib><creatorcontrib>KOBAYASHI, YOSHIYUKI</creatorcontrib><creatorcontrib>UEMURA, MASAYUKI</creatorcontrib><creatorcontrib>NAKAMURA, SHIN-ICHIRO</creatorcontrib><creatorcontrib>SATO, SHUICHIRO</creatorcontrib><creatorcontrib>HANAFUSA, TADASHI</creatorcontrib><creatorcontrib>YUMOTO, YASUHIRO</creatorcontrib><creatorcontrib>NAITO, ICHIRO</creatorcontrib><creatorcontrib>SHIRATORI, YASUSHI</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of gastroenterology and hepatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>FUJIWARA, KEISHI</au><au>HIGASHI, TOSHIHIRO</au><au>NOUSO, KAZUHIRO</au><au>NAKATSUKASA, HARUSHIGE</au><au>KOBAYASHI, YOSHIYUKI</au><au>UEMURA, MASAYUKI</au><au>NAKAMURA, SHIN-ICHIRO</au><au>SATO, SHUICHIRO</au><au>HANAFUSA, TADASHI</au><au>YUMOTO, YASUHIRO</au><au>NAITO, ICHIRO</au><au>SHIRATORI, YASUSHI</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Decreased expression of B7 costimulatory molecules and major histocompatibility complex class-I in human hepatocellular carcinoma</atitle><jtitle>Journal of gastroenterology and hepatology</jtitle><addtitle>J Gastroenterol Hepatol</addtitle><date>2004-10</date><risdate>2004</risdate><volume>19</volume><issue>10</issue><spage>1121</spage><epage>1127</epage><pages>1121-1127</pages><issn>0815-9319</issn><eissn>1440-1746</eissn><abstract>Background and Aim: We analyzed the expression of antigen‐processing and antigen‐presenting molecules in surgically resected fresh samples of human hepatocellular carcinoma (HCC) tissue to elucidate a mechanism of immune escape. We also examined the expression of interleukin (IL)‐10 protein, which might act to downregulate expression of antigen‐processing and antigen‐presenting molecules.
Methods: Twenty‐eight HCC samples obtained by surgical resection were analyzed for the expression of β2‐microglobulin, heat‐shock protein (HSP)‐70, human leukocyte antigen (HLA) class‐I, CD80 (B7‐1), CD86 (B7‐2) and IL‐10 by immunostaining.
Results: β2‐Microglobulin and HSP‐70 were preserved in all samples. In contrast, the expression of HLA class‐I molecules was significantly reduced according to lowering in the histological grading of tumor differentiation (P = 0.024). Furthermore, B7‐1 and B7‐2 expression was reduced in tumor cells compared with corresponding areas of liver tissue without malignant involvement irrespective of the histological grading of tumors (21% and 36%, respectively). Although IL‐10 protein was expressed in 54% of HCC, no relationship between the expression of IL‐10 and downregulation of B7‐1, B7‐2, and HLA class‐I was evident.
Conclusion: These findings suggest the potential role of B7 co‐stimulatory molecules and HLA class‐I molecules in facilitating HCC escape from immune surveillance without the involvement of IL‐10.</abstract><cop>Melbourne, Australia</cop><pub>Blackwell Science Pty</pub><pmid>15377288</pmid><doi>10.1111/j.1440-1746.2004.03467.x</doi><tpages>7</tpages></addata></record> |
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| subjects | Aged B7-1 Antigen - biosynthesis beta 2-Microglobulin - biosynthesis beta 2-Microglobulin - immunology Biological and medical sciences Carcinoma, Hepatocellular - immunology Female Gastroenterology. Liver. Pancreas. Abdomen Genes, MHC Class I - immunology HSP70 Heat-Shock Proteins - biosynthesis HSP70 Heat-Shock Proteins - immunology Humans immune tolerance Interleukin-10 - biosynthesis Interleukin-10 - immunology liver Liver Neoplasms - immunology Liver. Biliary tract. Portal circulation. Exocrine pancreas Male Medical sciences neoplasm tumor escape Tumors |
| title | Decreased expression of B7 costimulatory molecules and major histocompatibility complex class-I in human hepatocellular carcinoma |
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