Gene synthesis demystified
DNA fabrication of genetic cassettes at base-level precision is transforming genetic engineering from a laborious art to an information-driven discipline. Although substantial advances have been made in the development of DNA fabrication, the methods employed vary widely based on the length of the D...
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| Veröffentlicht in: | Trends in biotechnology (Regular ed.) 2009-02, Vol.27 (2), p.63-72 |
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| container_title | Trends in biotechnology (Regular ed.) |
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| creator | Czar, Michael J Anderson, J. Christopher Bader, Joel S Peccoud, Jean |
| description | DNA fabrication of genetic cassettes at base-level precision is transforming genetic engineering from a laborious art to an information-driven discipline. Although substantial advances have been made in the development of DNA fabrication, the methods employed vary widely based on the length of the DNA. All of these methods are available commercially, but can also be performed at the molecular biology bench using typical reagents and procedures. Because the technology is not mature and is still evolving rapidly, it is helpful to gain some understanding of the different steps in this process and the associated technical challenges to successfully take advantage of DNA fabrication in a research project. |
| doi_str_mv | 10.1016/j.tibtech.2008.10.007 |
| format | Article |
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Christopher</creatorcontrib><creatorcontrib>Bader, Joel S</creatorcontrib><creatorcontrib>Peccoud, Jean</creatorcontrib><title>Gene synthesis demystified</title><title>Trends in biotechnology (Regular ed.)</title><addtitle>Trends Biotechnol</addtitle><description>DNA fabrication of genetic cassettes at base-level precision is transforming genetic engineering from a laborious art to an information-driven discipline. Although substantial advances have been made in the development of DNA fabrication, the methods employed vary widely based on the length of the DNA. All of these methods are available commercially, but can also be performed at the molecular biology bench using typical reagents and procedures. Because the technology is not mature and is still evolving rapidly, it is helpful to gain some understanding of the different steps in this process and the associated technical challenges to successfully take advantage of DNA fabrication in a research project.</description><subject>Annealing</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Biotechnology industry</subject><subject>Cloning</subject><subject>Cloning, Molecular - methods</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA - genetics</subject><subject>Enzymes</subject><subject>Fabrication</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>Genetic engineering</subject><subject>Genetic Engineering - trends</subject><subject>Genetic technics</subject><subject>Internal Medicine</subject><subject>Methods. Procedures. 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| fulltext | fulltext |
| identifier | ISSN: 0167-7799 |
| ispartof | Trends in biotechnology (Regular ed.), 2009-02, Vol.27 (2), p.63-72 |
| issn | 0167-7799 1879-3096 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Annealing Biological and medical sciences Biotechnology Biotechnology industry Cloning Cloning, Molecular - methods Deoxyribonucleic acid DNA DNA - genetics Enzymes Fabrication Fundamental and applied biological sciences. Psychology Genes Genetic engineering Genetic Engineering - trends Genetic technics Internal Medicine Methods. Procedures. Technologies Molecular biology Mutation Polymerase Chain Reaction - trends Protocol Reagents Research projects Sequence Analysis, DNA - trends Software - trends Synthetic digonucleotides and genes. Sequencing |
| title | Gene synthesis demystified |
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