The mucosal adjuvanticity of the oligodeoxynucleotides containing a non-methylated CpG motif on BCG and diphtheria toxoid
Abstract CpG-DNA is currently attracting attention as an effective and safe vaccine adjuvant to prevent from microbial infections. In this report, we examined the effects of oligo B, which is a synthetic CpG-DNA, in mucosal administration of Bacillus Calmette-Guérin (BCG) and diphtheria toxoid (DT)....
Gespeichert in:
| Veröffentlicht in: | Vaccine 2009-02, Vol.27 (8), p.1166-1173 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1173 |
|---|---|
| container_issue | 8 |
| container_start_page | 1166 |
| container_title | Vaccine |
| container_volume | 27 |
| creator | Maeyama, Jun-ichi Komiya, Takako Takahashi, Motohide Isaka, Masanori Goto, Norihisa Yamamoto, Saburo |
| description | Abstract CpG-DNA is currently attracting attention as an effective and safe vaccine adjuvant to prevent from microbial infections. In this report, we examined the effects of oligo B, which is a synthetic CpG-DNA, in mucosal administration of Bacillus Calmette-Guérin (BCG) and diphtheria toxoid (DT). Co-administration with oligo B enhanced BCG-induced delayed type hypersensitivity to purified protein derivative (PPD) in guinea pigs. The titers of anti-DT serum IgG, IgA and mucosal IgA antibodies induced by intranasal administration with DT plus oligo B were significantly higher than that with DT alone. In both C57BL/6 and BALB/c mice, intranasal administration of DT with oligo B induced enough level of antibodies to prevent onset of diphtheria. The analysis of antibody subclasses showed that intranasal administration of oligo B induced not only IgG1 but also IgG2a, IgG2c and IgA anti-DT antibodies. In contrast, there was no or little production of the anti-DT serum IgE. Taken together our data suggest that oligo B is a powerful adjuvant in mucosal immunization. |
| doi_str_mv | 10.1016/j.vaccine.2008.12.025 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_66885062</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>1_s2_0_S0264410X08017532</els_id><sourcerecordid>3476780261</sourcerecordid><originalsourceid>FETCH-LOGICAL-c573t-cda9876600f3ca6b686cf458571c57ead7c25649d82fa05f06bae312808c53fd3</originalsourceid><addsrcrecordid>eNqFksGO0zAQhiMEYsvCI4AsIbilzNix41xAUEFBWokDi8TNcm1n65LY3TipNm-Pq1astJc9-eBvPnvmn6J4jbBEQPFhtzxoY3xwSwogl0iXQPmTYoGyZiXlKJ8WC6CiKiuEPxfFi5R2AMAZNs-LC2yQCahgUczXW0f6ycSkO6LtbjroMHrjx5nEloz5Mnb-JloX7-Ywmc7F0VuXiIlh1D74cEM0CTGUvRu3c6dHZ8lqvyZ95loSA_myWhMdLLF-v826wWsyxrvo7cviWau75F6dz8vi97ev16vv5dXP9Y_V56vS8JqNpbG6kbUQAC0zWmyEFKatuOQ1ZsBpWxvKRdVYSVsNvAWx0Y4hlSANZ61ll8X7k3c_xNvJpVH1PhnXdTq4OCUlhJQcBH0UpMCaBqs6g28fgLs4DSE3oVCglAxBikzxE2WGmNLgWrUffK-HWSGoY4Rqp84RqmOECqnKEea6N2f7tOmdva86Z5aBd2dAJ6O7dtDB-PSfo5gliEfRpxPn8nQP3g0qGe-CcdYPzozKRv_oVz4-MJguZ54f_etml-67VikXqF_HfTuuG0jAmjPK_gFTPtHs</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1618831086</pqid></control><display><type>article</type><title>The mucosal adjuvanticity of the oligodeoxynucleotides containing a non-methylated CpG motif on BCG and diphtheria toxoid</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><source>ProQuest Central</source><creator>Maeyama, Jun-ichi ; Komiya, Takako ; Takahashi, Motohide ; Isaka, Masanori ; Goto, Norihisa ; Yamamoto, Saburo</creator><creatorcontrib>Maeyama, Jun-ichi ; Komiya, Takako ; Takahashi, Motohide ; Isaka, Masanori ; Goto, Norihisa ; Yamamoto, Saburo</creatorcontrib><description>Abstract CpG-DNA is currently attracting attention as an effective and safe vaccine adjuvant to prevent from microbial infections. In this report, we examined the effects of oligo B, which is a synthetic CpG-DNA, in mucosal administration of Bacillus Calmette-Guérin (BCG) and diphtheria toxoid (DT). Co-administration with oligo B enhanced BCG-induced delayed type hypersensitivity to purified protein derivative (PPD) in guinea pigs. The titers of anti-DT serum IgG, IgA and mucosal IgA antibodies induced by intranasal administration with DT plus oligo B were significantly higher than that with DT alone. In both C57BL/6 and BALB/c mice, intranasal administration of DT with oligo B induced enough level of antibodies to prevent onset of diphtheria. The analysis of antibody subclasses showed that intranasal administration of oligo B induced not only IgG1 but also IgG2a, IgG2c and IgA anti-DT antibodies. In contrast, there was no or little production of the anti-DT serum IgE. Taken together our data suggest that oligo B is a powerful adjuvant in mucosal immunization.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/j.vaccine.2008.12.025</identifier><identifier>PMID: 19136040</identifier><identifier>CODEN: VACCDE</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Adjuvants, Immunologic - administration & dosage ; Adjuvants, Immunologic - pharmacology ; Administration, Intranasal ; Allergy and Immunology ; Animals ; Antibodies, Bacterial - analysis ; Antibodies, Bacterial - blood ; Applied microbiology ; Bacterial diseases ; Bioassays ; Biological and medical sciences ; CpG-DNA ; Cytokines ; Cytokines - secretion ; Cytotoxicity ; Deoxyribonucleic acid ; Diphtheria Toxoid - administration & dosage ; Diphtheria Toxoid - immunology ; DNA ; Ent and stomatologic bacterial diseases ; Female ; Fundamental and applied biological sciences. Psychology ; Guinea Pigs ; Human bacterial diseases ; Hypersensitivity ; Immune system ; Immunity, Mucosal ; Immunization ; Immunoglobulin A - analysis ; Immunoglobulin A - blood ; Immunoglobulin E - blood ; Immunoglobulin G - blood ; Infectious diseases ; Leukocytes, Mononuclear - immunology ; Medical sciences ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Microbiology ; Mucosal adjuvant ; Mucosal vaccine ; Mucous Membrane - immunology ; Mycobacterium bovis - immunology ; Oligodeoxyribonucleotides - administration & dosage ; Oligodeoxyribonucleotides - pharmacology ; Proteins ; Rodents ; Spleen ; Spleen - immunology ; Tuberculin Test ; Tuberculosis and atypical mycobacterial infections ; Vaccines ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) ; Viral infections</subject><ispartof>Vaccine, 2009-02, Vol.27 (8), p.1166-1173</ispartof><rights>Elsevier Ltd</rights><rights>2008 Elsevier Ltd</rights><rights>2009 INIST-CNRS</rights><rights>Copyright Elsevier Limited Feb 18, 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c573t-cda9876600f3ca6b686cf458571c57ead7c25649d82fa05f06bae312808c53fd3</citedby><cites>FETCH-LOGICAL-c573t-cda9876600f3ca6b686cf458571c57ead7c25649d82fa05f06bae312808c53fd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/1618831086?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974,64362,64364,64366,72216</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21253115$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19136040$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Maeyama, Jun-ichi</creatorcontrib><creatorcontrib>Komiya, Takako</creatorcontrib><creatorcontrib>Takahashi, Motohide</creatorcontrib><creatorcontrib>Isaka, Masanori</creatorcontrib><creatorcontrib>Goto, Norihisa</creatorcontrib><creatorcontrib>Yamamoto, Saburo</creatorcontrib><title>The mucosal adjuvanticity of the oligodeoxynucleotides containing a non-methylated CpG motif on BCG and diphtheria toxoid</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>Abstract CpG-DNA is currently attracting attention as an effective and safe vaccine adjuvant to prevent from microbial infections. In this report, we examined the effects of oligo B, which is a synthetic CpG-DNA, in mucosal administration of Bacillus Calmette-Guérin (BCG) and diphtheria toxoid (DT). Co-administration with oligo B enhanced BCG-induced delayed type hypersensitivity to purified protein derivative (PPD) in guinea pigs. The titers of anti-DT serum IgG, IgA and mucosal IgA antibodies induced by intranasal administration with DT plus oligo B were significantly higher than that with DT alone. In both C57BL/6 and BALB/c mice, intranasal administration of DT with oligo B induced enough level of antibodies to prevent onset of diphtheria. The analysis of antibody subclasses showed that intranasal administration of oligo B induced not only IgG1 but also IgG2a, IgG2c and IgA anti-DT antibodies. In contrast, there was no or little production of the anti-DT serum IgE. Taken together our data suggest that oligo B is a powerful adjuvant in mucosal immunization.</description><subject>Adjuvants, Immunologic - administration & dosage</subject><subject>Adjuvants, Immunologic - pharmacology</subject><subject>Administration, Intranasal</subject><subject>Allergy and Immunology</subject><subject>Animals</subject><subject>Antibodies, Bacterial - analysis</subject><subject>Antibodies, Bacterial - blood</subject><subject>Applied microbiology</subject><subject>Bacterial diseases</subject><subject>Bioassays</subject><subject>Biological and medical sciences</subject><subject>CpG-DNA</subject><subject>Cytokines</subject><subject>Cytokines - secretion</subject><subject>Cytotoxicity</subject><subject>Deoxyribonucleic acid</subject><subject>Diphtheria Toxoid - administration & dosage</subject><subject>Diphtheria Toxoid - immunology</subject><subject>DNA</subject><subject>Ent and stomatologic bacterial diseases</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Guinea Pigs</subject><subject>Human bacterial diseases</subject><subject>Hypersensitivity</subject><subject>Immune system</subject><subject>Immunity, Mucosal</subject><subject>Immunization</subject><subject>Immunoglobulin A - analysis</subject><subject>Immunoglobulin A - blood</subject><subject>Immunoglobulin E - blood</subject><subject>Immunoglobulin G - blood</subject><subject>Infectious diseases</subject><subject>Leukocytes, Mononuclear - immunology</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Inbred C57BL</subject><subject>Microbiology</subject><subject>Mucosal adjuvant</subject><subject>Mucosal vaccine</subject><subject>Mucous Membrane - immunology</subject><subject>Mycobacterium bovis - immunology</subject><subject>Oligodeoxyribonucleotides - administration & dosage</subject><subject>Oligodeoxyribonucleotides - pharmacology</subject><subject>Proteins</subject><subject>Rodents</subject><subject>Spleen</subject><subject>Spleen - immunology</subject><subject>Tuberculin Test</subject><subject>Tuberculosis and atypical mycobacterial infections</subject><subject>Vaccines</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><subject>Viral infections</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFksGO0zAQhiMEYsvCI4AsIbilzNix41xAUEFBWokDi8TNcm1n65LY3TipNm-Pq1astJc9-eBvPnvmn6J4jbBEQPFhtzxoY3xwSwogl0iXQPmTYoGyZiXlKJ8WC6CiKiuEPxfFi5R2AMAZNs-LC2yQCahgUczXW0f6ycSkO6LtbjroMHrjx5nEloz5Mnb-JloX7-Ywmc7F0VuXiIlh1D74cEM0CTGUvRu3c6dHZ8lqvyZ95loSA_myWhMdLLF-v826wWsyxrvo7cviWau75F6dz8vi97ev16vv5dXP9Y_V56vS8JqNpbG6kbUQAC0zWmyEFKatuOQ1ZsBpWxvKRdVYSVsNvAWx0Y4hlSANZ61ll8X7k3c_xNvJpVH1PhnXdTq4OCUlhJQcBH0UpMCaBqs6g28fgLs4DSE3oVCglAxBikzxE2WGmNLgWrUffK-HWSGoY4Rqp84RqmOECqnKEea6N2f7tOmdva86Z5aBd2dAJ6O7dtDB-PSfo5gliEfRpxPn8nQP3g0qGe-CcdYPzozKRv_oVz4-MJguZ54f_etml-67VikXqF_HfTuuG0jAmjPK_gFTPtHs</recordid><startdate>20090218</startdate><enddate>20090218</enddate><creator>Maeyama, Jun-ichi</creator><creator>Komiya, Takako</creator><creator>Takahashi, Motohide</creator><creator>Isaka, Masanori</creator><creator>Goto, Norihisa</creator><creator>Yamamoto, Saburo</creator><general>Elsevier Ltd</general><general>Elsevier</general><general>Elsevier Limited</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7RV</scope><scope>7T2</scope><scope>7T5</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88C</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0R</scope><scope>M0S</scope><scope>M0T</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20090218</creationdate><title>The mucosal adjuvanticity of the oligodeoxynucleotides containing a non-methylated CpG motif on BCG and diphtheria toxoid</title><author>Maeyama, Jun-ichi ; Komiya, Takako ; Takahashi, Motohide ; Isaka, Masanori ; Goto, Norihisa ; Yamamoto, Saburo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c573t-cda9876600f3ca6b686cf458571c57ead7c25649d82fa05f06bae312808c53fd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Adjuvants, Immunologic - administration & dosage</topic><topic>Adjuvants, Immunologic - pharmacology</topic><topic>Administration, Intranasal</topic><topic>Allergy and Immunology</topic><topic>Animals</topic><topic>Antibodies, Bacterial - analysis</topic><topic>Antibodies, Bacterial - blood</topic><topic>Applied microbiology</topic><topic>Bacterial diseases</topic><topic>Bioassays</topic><topic>Biological and medical sciences</topic><topic>CpG-DNA</topic><topic>Cytokines</topic><topic>Cytokines - secretion</topic><topic>Cytotoxicity</topic><topic>Deoxyribonucleic acid</topic><topic>Diphtheria Toxoid - administration & dosage</topic><topic>Diphtheria Toxoid - immunology</topic><topic>DNA</topic><topic>Ent and stomatologic bacterial diseases</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Guinea Pigs</topic><topic>Human bacterial diseases</topic><topic>Hypersensitivity</topic><topic>Immune system</topic><topic>Immunity, Mucosal</topic><topic>Immunization</topic><topic>Immunoglobulin A - analysis</topic><topic>Immunoglobulin A - blood</topic><topic>Immunoglobulin E - blood</topic><topic>Immunoglobulin G - blood</topic><topic>Infectious diseases</topic><topic>Leukocytes, Mononuclear - immunology</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Inbred C57BL</topic><topic>Microbiology</topic><topic>Mucosal adjuvant</topic><topic>Mucosal vaccine</topic><topic>Mucous Membrane - immunology</topic><topic>Mycobacterium bovis - immunology</topic><topic>Oligodeoxyribonucleotides - administration & dosage</topic><topic>Oligodeoxyribonucleotides - pharmacology</topic><topic>Proteins</topic><topic>Rodents</topic><topic>Spleen</topic><topic>Spleen - immunology</topic><topic>Tuberculin Test</topic><topic>Tuberculosis and atypical mycobacterial infections</topic><topic>Vaccines</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</topic><topic>Viral infections</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Maeyama, Jun-ichi</creatorcontrib><creatorcontrib>Komiya, Takako</creatorcontrib><creatorcontrib>Takahashi, Motohide</creatorcontrib><creatorcontrib>Isaka, Masanori</creatorcontrib><creatorcontrib>Goto, Norihisa</creatorcontrib><creatorcontrib>Yamamoto, Saburo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nursing & Allied Health Database</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Healthcare Administration Database (Alumni)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>ProQuest Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Healthcare Administration Database</collection><collection>Medical Database</collection><collection>ProQuest research library</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Maeyama, Jun-ichi</au><au>Komiya, Takako</au><au>Takahashi, Motohide</au><au>Isaka, Masanori</au><au>Goto, Norihisa</au><au>Yamamoto, Saburo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The mucosal adjuvanticity of the oligodeoxynucleotides containing a non-methylated CpG motif on BCG and diphtheria toxoid</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2009-02-18</date><risdate>2009</risdate><volume>27</volume><issue>8</issue><spage>1166</spage><epage>1173</epage><pages>1166-1173</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><coden>VACCDE</coden><abstract>Abstract CpG-DNA is currently attracting attention as an effective and safe vaccine adjuvant to prevent from microbial infections. In this report, we examined the effects of oligo B, which is a synthetic CpG-DNA, in mucosal administration of Bacillus Calmette-Guérin (BCG) and diphtheria toxoid (DT). Co-administration with oligo B enhanced BCG-induced delayed type hypersensitivity to purified protein derivative (PPD) in guinea pigs. The titers of anti-DT serum IgG, IgA and mucosal IgA antibodies induced by intranasal administration with DT plus oligo B were significantly higher than that with DT alone. In both C57BL/6 and BALB/c mice, intranasal administration of DT with oligo B induced enough level of antibodies to prevent onset of diphtheria. The analysis of antibody subclasses showed that intranasal administration of oligo B induced not only IgG1 but also IgG2a, IgG2c and IgA anti-DT antibodies. In contrast, there was no or little production of the anti-DT serum IgE. Taken together our data suggest that oligo B is a powerful adjuvant in mucosal immunization.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>19136040</pmid><doi>10.1016/j.vaccine.2008.12.025</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0264-410X |
| ispartof | Vaccine, 2009-02, Vol.27 (8), p.1166-1173 |
| issn | 0264-410X 1873-2518 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_66885062 |
| source | MEDLINE; Elsevier ScienceDirect Journals; ProQuest Central |
| subjects | Adjuvants, Immunologic - administration & dosage Adjuvants, Immunologic - pharmacology Administration, Intranasal Allergy and Immunology Animals Antibodies, Bacterial - analysis Antibodies, Bacterial - blood Applied microbiology Bacterial diseases Bioassays Biological and medical sciences CpG-DNA Cytokines Cytokines - secretion Cytotoxicity Deoxyribonucleic acid Diphtheria Toxoid - administration & dosage Diphtheria Toxoid - immunology DNA Ent and stomatologic bacterial diseases Female Fundamental and applied biological sciences. Psychology Guinea Pigs Human bacterial diseases Hypersensitivity Immune system Immunity, Mucosal Immunization Immunoglobulin A - analysis Immunoglobulin A - blood Immunoglobulin E - blood Immunoglobulin G - blood Infectious diseases Leukocytes, Mononuclear - immunology Medical sciences Mice Mice, Inbred BALB C Mice, Inbred C57BL Microbiology Mucosal adjuvant Mucosal vaccine Mucous Membrane - immunology Mycobacterium bovis - immunology Oligodeoxyribonucleotides - administration & dosage Oligodeoxyribonucleotides - pharmacology Proteins Rodents Spleen Spleen - immunology Tuberculin Test Tuberculosis and atypical mycobacterial infections Vaccines Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Viral infections |
| title | The mucosal adjuvanticity of the oligodeoxynucleotides containing a non-methylated CpG motif on BCG and diphtheria toxoid |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-22T15%3A58%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20mucosal%20adjuvanticity%20of%20the%20oligodeoxynucleotides%20containing%20a%20non-methylated%20CpG%20motif%20on%20BCG%20and%20diphtheria%20toxoid&rft.jtitle=Vaccine&rft.au=Maeyama,%20Jun-ichi&rft.date=2009-02-18&rft.volume=27&rft.issue=8&rft.spage=1166&rft.epage=1173&rft.pages=1166-1173&rft.issn=0264-410X&rft.eissn=1873-2518&rft.coden=VACCDE&rft_id=info:doi/10.1016/j.vaccine.2008.12.025&rft_dat=%3Cproquest_cross%3E3476780261%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1618831086&rft_id=info:pmid/19136040&rft_els_id=1_s2_0_S0264410X08017532&rfr_iscdi=true |