Identification of hydroxyl radicals after UV irradiation of aqueous extracts of cigarette smoke and evaluation of urinary 8-OHdG in smokers
The role of oxidative stress in tobacco smoke damage was examined using a combination of in vitro and in vivo approaches. Aqueous extracts of cigarette smoke were irradiated with UV and hydroxyl radical generation was evaluated by electron spin resonance (ESR). The spectra obtained revealed spin add...
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| Veröffentlicht in: | Asian Pacific journal of cancer prevention : APJCP 2004-07, Vol.5 (3), p.279-283 |
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| container_title | Asian Pacific journal of cancer prevention : APJCP |
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| creator | Yatsuzuka, Miki Tazawa, Kenji Yasuda, Tomomi Yoshii, Miho Ogawa, Kouhei Wada, Shigeto |
| description | The role of oxidative stress in tobacco smoke damage was examined using a combination of in vitro and in vivo approaches. Aqueous extracts of cigarette smoke were irradiated with UV and hydroxyl radical generation was evaluated by electron spin resonance (ESR). The spectra obtained revealed spin adducts of the hydroxyl radical (*OH) to increase with the volume of the aqueous extract of cigarette smoke, cigarette smoke collection flow, and UV irradiation time. Hydroxyl radical generation persisted for long hours, showing no change over time. Ascorbic acid protected against radical generation in a marked concentration-dependent fashion. In specimens of urine from volunteers, 8-OHdG levels and the 8-OHdG-production rate per hour were found to be higher in smokers than non-smokers. Our results suggest that more marked oxidative stress occurs with the smoking habit. |
| format | Article |
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| issn | 1513-7368 |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Free E- Journals |
| subjects | Antioxidants - pharmacology Ascorbic Acid - pharmacology Deoxyguanosine - analogs & derivatives Deoxyguanosine - urine DNA Damage Electron Spin Resonance Spectroscopy Free Radicals - analysis Humans Oxidative Stress Smoking - adverse effects Ultraviolet Rays |
| title | Identification of hydroxyl radicals after UV irradiation of aqueous extracts of cigarette smoke and evaluation of urinary 8-OHdG in smokers |
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