Association of methylenetetrahydrofolate reductase T677 allele with early development of chronic allograft nephropathy
Objectives: The aim of this study was to evaluate the role of genetic polymorphisms on the development of chronic allograft nephropathy (CAN). Design and methods: Using the polymerase chain reaction (PCR), polymorphisms of methylenetetrahydrofolate reductase (MTHFR C677T), interleukin-6 (IL-6 G-174C...
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| Veröffentlicht in: | Clinical biochemistry 2004-10, Vol.37 (10), p.919-924 |
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| creator | Viklický, Ondřej Hubáček, Jaroslav A. Kvasnička, Jan Matl, Ivo Voska, Luděk Skibová, Jelena Teplan, Vladimı́r Vı́tko, Štefan |
| description | Objectives: The aim of this study was to evaluate the role of genetic polymorphisms on the development of chronic allograft nephropathy (CAN).
Design and methods: Using the polymerase chain reaction (PCR), polymorphisms of methylenetetrahydrofolate reductase (MTHFR C677T), interleukin-6 (IL-6 G-174C) and CD14 (C-260T) were evaluated in 92 kidney transplant recipients with stable renal graft function and no signs of acute rejection in a protocol that included graft biopsy at 12 months after kidney transplantation. A normal population sample (
n = 365) was also included. Multivariate analysis was used to evaluate the effect of different variables on the CAN appearance.
Results: There were no differences in alleles and genotypes distribution between transplant group and normal population sample. The CAN+ group (
n = 69) significantly differed from CAN− (
n = 23) in both MTHFR (
P < 0.05) and IL-6 (
P < 0.01) genotype distribution. Using logistic regression multivariate analysis, MTHFR T677 allele (OR: 3.91, CI: 1.11–13.8;
P < 0.05), patient age (OR: 0.94, CI: 0.88–0.98;
P < 0.01) and proteinuria (OR: 3.63, CI: 1.25–10.6;
P < 0.05) were associated with CAN. Although the IL-6 G-174 allele was shown to be associated with CAN development in univariate analysis (
P < 0.01), the multivariate analysis did not show an association. There was no relation between CD14 gene polymorphism and CAN.
Conclusion: The MTHFR T677 allele is associated with the presence of CAN in kidney graft biopsies 12 months after transplantation. |
| doi_str_mv | 10.1016/j.clinbiochem.2004.05.022 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_66878297</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0009912004001456</els_id><sourcerecordid>66878297</sourcerecordid><originalsourceid>FETCH-LOGICAL-c402t-5d30de2281727415d5f92009384a8dec1f0eddc1ba4a40329b34098f714bf4bd3</originalsourceid><addsrcrecordid>eNqNkE2P0zAQhi0EYsvCX0Dhwi3BX4nj46piAWklLsvZcuwxceXEwXaL-u9x1Upw5DSa0TPzah6EPhDcEUyGT4fOBL9OPpoZlo5izDvcd5jSF2hHRsFaKhl7iXYYY9lKQvEdepPzobaUj8NrdEd6NkhB-Q6dHnKOxuvi49pE1yxQ5nOAFQqUpOezTdHFoAs0CezRFJ2heR6EaHQIEKD57cvcgE7h3Fg4QYjbAmu5XDJziqs3FzD-TNqVZoWtzjZdE96iV06HDO9u9R79ePz8vP_aPn3_8m3_8NQajmlpe8uwBUpHIqjgpLe9k_VdyUauRwuGOAzWGjJprjlmVE6MYzk6Qfjk-GTZPfp4vbul-OsIuajFZwMh6BXiMathGMVIpaigvIImxZwTOLUlv-h0VgSri3R1UP9IVxfpCveqSq-7728hx2kB-3fzZrkC-ysA9dWTh6Sy8bAasD6BKcpG_x8xfwAggZvm</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>66878297</pqid></control><display><type>article</type><title>Association of methylenetetrahydrofolate reductase T677 allele with early development of chronic allograft nephropathy</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Viklický, Ondřej ; Hubáček, Jaroslav A. ; Kvasnička, Jan ; Matl, Ivo ; Voska, Luděk ; Skibová, Jelena ; Teplan, Vladimı́r ; Vı́tko, Štefan</creator><creatorcontrib>Viklický, Ondřej ; Hubáček, Jaroslav A. ; Kvasnička, Jan ; Matl, Ivo ; Voska, Luděk ; Skibová, Jelena ; Teplan, Vladimı́r ; Vı́tko, Štefan</creatorcontrib><description><![CDATA[Objectives: The aim of this study was to evaluate the role of genetic polymorphisms on the development of chronic allograft nephropathy (CAN).
Design and methods: Using the polymerase chain reaction (PCR), polymorphisms of methylenetetrahydrofolate reductase (MTHFR C677T), interleukin-6 (IL-6 G-174C) and CD14 (C-260T) were evaluated in 92 kidney transplant recipients with stable renal graft function and no signs of acute rejection in a protocol that included graft biopsy at 12 months after kidney transplantation. A normal population sample (
n = 365) was also included. Multivariate analysis was used to evaluate the effect of different variables on the CAN appearance.
Results: There were no differences in alleles and genotypes distribution between transplant group and normal population sample. The CAN+ group (
n = 69) significantly differed from CAN− (
n = 23) in both MTHFR (
P < 0.05) and IL-6 (
P < 0.01) genotype distribution. Using logistic regression multivariate analysis, MTHFR T677 allele (OR: 3.91, CI: 1.11–13.8;
P < 0.05), patient age (OR: 0.94, CI: 0.88–0.98;
P < 0.01) and proteinuria (OR: 3.63, CI: 1.25–10.6;
P < 0.05) were associated with CAN. Although the IL-6 G-174 allele was shown to be associated with CAN development in univariate analysis (
P < 0.01), the multivariate analysis did not show an association. There was no relation between CD14 gene polymorphism and CAN.
Conclusion: The MTHFR T677 allele is associated with the presence of CAN in kidney graft biopsies 12 months after transplantation.]]></description><identifier>ISSN: 0009-9120</identifier><identifier>EISSN: 1873-2933</identifier><identifier>DOI: 10.1016/j.clinbiochem.2004.05.022</identifier><identifier>PMID: 15369724</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Alleles ; CD14 ; Chronic allograft nephropathy ; Chronic Disease ; Female ; Gene polymorphism ; Genetic Predisposition to Disease ; Graft vs Host Disease - enzymology ; Graft vs Host Disease - genetics ; Humans ; IL-6 ; Interleukin-6 - genetics ; Kidney Diseases - enzymology ; Kidney Diseases - genetics ; Kidney Transplantation ; Lipopolysaccharide Receptors - genetics ; Male ; Methylenetetrahydrofolate Reductase (NADPH2) - genetics ; Middle Aged ; MTHFR ; Polymorphism, Genetic ; Proteinuria ; Transplantation, Homologous</subject><ispartof>Clinical biochemistry, 2004-10, Vol.37 (10), p.919-924</ispartof><rights>2004 The Canadian Society of Clinical Chemists</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c402t-5d30de2281727415d5f92009384a8dec1f0eddc1ba4a40329b34098f714bf4bd3</citedby><cites>FETCH-LOGICAL-c402t-5d30de2281727415d5f92009384a8dec1f0eddc1ba4a40329b34098f714bf4bd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.clinbiochem.2004.05.022$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15369724$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Viklický, Ondřej</creatorcontrib><creatorcontrib>Hubáček, Jaroslav A.</creatorcontrib><creatorcontrib>Kvasnička, Jan</creatorcontrib><creatorcontrib>Matl, Ivo</creatorcontrib><creatorcontrib>Voska, Luděk</creatorcontrib><creatorcontrib>Skibová, Jelena</creatorcontrib><creatorcontrib>Teplan, Vladimı́r</creatorcontrib><creatorcontrib>Vı́tko, Štefan</creatorcontrib><title>Association of methylenetetrahydrofolate reductase T677 allele with early development of chronic allograft nephropathy</title><title>Clinical biochemistry</title><addtitle>Clin Biochem</addtitle><description><![CDATA[Objectives: The aim of this study was to evaluate the role of genetic polymorphisms on the development of chronic allograft nephropathy (CAN).
Design and methods: Using the polymerase chain reaction (PCR), polymorphisms of methylenetetrahydrofolate reductase (MTHFR C677T), interleukin-6 (IL-6 G-174C) and CD14 (C-260T) were evaluated in 92 kidney transplant recipients with stable renal graft function and no signs of acute rejection in a protocol that included graft biopsy at 12 months after kidney transplantation. A normal population sample (
n = 365) was also included. Multivariate analysis was used to evaluate the effect of different variables on the CAN appearance.
Results: There were no differences in alleles and genotypes distribution between transplant group and normal population sample. The CAN+ group (
n = 69) significantly differed from CAN− (
n = 23) in both MTHFR (
P < 0.05) and IL-6 (
P < 0.01) genotype distribution. Using logistic regression multivariate analysis, MTHFR T677 allele (OR: 3.91, CI: 1.11–13.8;
P < 0.05), patient age (OR: 0.94, CI: 0.88–0.98;
P < 0.01) and proteinuria (OR: 3.63, CI: 1.25–10.6;
P < 0.05) were associated with CAN. Although the IL-6 G-174 allele was shown to be associated with CAN development in univariate analysis (
P < 0.01), the multivariate analysis did not show an association. There was no relation between CD14 gene polymorphism and CAN.
Conclusion: The MTHFR T677 allele is associated with the presence of CAN in kidney graft biopsies 12 months after transplantation.]]></description><subject>Alleles</subject><subject>CD14</subject><subject>Chronic allograft nephropathy</subject><subject>Chronic Disease</subject><subject>Female</subject><subject>Gene polymorphism</subject><subject>Genetic Predisposition to Disease</subject><subject>Graft vs Host Disease - enzymology</subject><subject>Graft vs Host Disease - genetics</subject><subject>Humans</subject><subject>IL-6</subject><subject>Interleukin-6 - genetics</subject><subject>Kidney Diseases - enzymology</subject><subject>Kidney Diseases - genetics</subject><subject>Kidney Transplantation</subject><subject>Lipopolysaccharide Receptors - genetics</subject><subject>Male</subject><subject>Methylenetetrahydrofolate Reductase (NADPH2) - genetics</subject><subject>Middle Aged</subject><subject>MTHFR</subject><subject>Polymorphism, Genetic</subject><subject>Proteinuria</subject><subject>Transplantation, Homologous</subject><issn>0009-9120</issn><issn>1873-2933</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE2P0zAQhi0EYsvCX0Dhwi3BX4nj46piAWklLsvZcuwxceXEwXaL-u9x1Upw5DSa0TPzah6EPhDcEUyGT4fOBL9OPpoZlo5izDvcd5jSF2hHRsFaKhl7iXYYY9lKQvEdepPzobaUj8NrdEd6NkhB-Q6dHnKOxuvi49pE1yxQ5nOAFQqUpOezTdHFoAs0CezRFJ2heR6EaHQIEKD57cvcgE7h3Fg4QYjbAmu5XDJziqs3FzD-TNqVZoWtzjZdE96iV06HDO9u9R79ePz8vP_aPn3_8m3_8NQajmlpe8uwBUpHIqjgpLe9k_VdyUauRwuGOAzWGjJprjlmVE6MYzk6Qfjk-GTZPfp4vbul-OsIuajFZwMh6BXiMathGMVIpaigvIImxZwTOLUlv-h0VgSri3R1UP9IVxfpCveqSq-7728hx2kB-3fzZrkC-ysA9dWTh6Sy8bAasD6BKcpG_x8xfwAggZvm</recordid><startdate>20041001</startdate><enddate>20041001</enddate><creator>Viklický, Ondřej</creator><creator>Hubáček, Jaroslav A.</creator><creator>Kvasnička, Jan</creator><creator>Matl, Ivo</creator><creator>Voska, Luděk</creator><creator>Skibová, Jelena</creator><creator>Teplan, Vladimı́r</creator><creator>Vı́tko, Štefan</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20041001</creationdate><title>Association of methylenetetrahydrofolate reductase T677 allele with early development of chronic allograft nephropathy</title><author>Viklický, Ondřej ; Hubáček, Jaroslav A. ; Kvasnička, Jan ; Matl, Ivo ; Voska, Luděk ; Skibová, Jelena ; Teplan, Vladimı́r ; Vı́tko, Štefan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c402t-5d30de2281727415d5f92009384a8dec1f0eddc1ba4a40329b34098f714bf4bd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Alleles</topic><topic>CD14</topic><topic>Chronic allograft nephropathy</topic><topic>Chronic Disease</topic><topic>Female</topic><topic>Gene polymorphism</topic><topic>Genetic Predisposition to Disease</topic><topic>Graft vs Host Disease - enzymology</topic><topic>Graft vs Host Disease - genetics</topic><topic>Humans</topic><topic>IL-6</topic><topic>Interleukin-6 - genetics</topic><topic>Kidney Diseases - enzymology</topic><topic>Kidney Diseases - genetics</topic><topic>Kidney Transplantation</topic><topic>Lipopolysaccharide Receptors - genetics</topic><topic>Male</topic><topic>Methylenetetrahydrofolate Reductase (NADPH2) - genetics</topic><topic>Middle Aged</topic><topic>MTHFR</topic><topic>Polymorphism, Genetic</topic><topic>Proteinuria</topic><topic>Transplantation, Homologous</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Viklický, Ondřej</creatorcontrib><creatorcontrib>Hubáček, Jaroslav A.</creatorcontrib><creatorcontrib>Kvasnička, Jan</creatorcontrib><creatorcontrib>Matl, Ivo</creatorcontrib><creatorcontrib>Voska, Luděk</creatorcontrib><creatorcontrib>Skibová, Jelena</creatorcontrib><creatorcontrib>Teplan, Vladimı́r</creatorcontrib><creatorcontrib>Vı́tko, Štefan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Viklický, Ondřej</au><au>Hubáček, Jaroslav A.</au><au>Kvasnička, Jan</au><au>Matl, Ivo</au><au>Voska, Luděk</au><au>Skibová, Jelena</au><au>Teplan, Vladimı́r</au><au>Vı́tko, Štefan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Association of methylenetetrahydrofolate reductase T677 allele with early development of chronic allograft nephropathy</atitle><jtitle>Clinical biochemistry</jtitle><addtitle>Clin Biochem</addtitle><date>2004-10-01</date><risdate>2004</risdate><volume>37</volume><issue>10</issue><spage>919</spage><epage>924</epage><pages>919-924</pages><issn>0009-9120</issn><eissn>1873-2933</eissn><abstract><![CDATA[Objectives: The aim of this study was to evaluate the role of genetic polymorphisms on the development of chronic allograft nephropathy (CAN).
Design and methods: Using the polymerase chain reaction (PCR), polymorphisms of methylenetetrahydrofolate reductase (MTHFR C677T), interleukin-6 (IL-6 G-174C) and CD14 (C-260T) were evaluated in 92 kidney transplant recipients with stable renal graft function and no signs of acute rejection in a protocol that included graft biopsy at 12 months after kidney transplantation. A normal population sample (
n = 365) was also included. Multivariate analysis was used to evaluate the effect of different variables on the CAN appearance.
Results: There were no differences in alleles and genotypes distribution between transplant group and normal population sample. The CAN+ group (
n = 69) significantly differed from CAN− (
n = 23) in both MTHFR (
P < 0.05) and IL-6 (
P < 0.01) genotype distribution. Using logistic regression multivariate analysis, MTHFR T677 allele (OR: 3.91, CI: 1.11–13.8;
P < 0.05), patient age (OR: 0.94, CI: 0.88–0.98;
P < 0.01) and proteinuria (OR: 3.63, CI: 1.25–10.6;
P < 0.05) were associated with CAN. Although the IL-6 G-174 allele was shown to be associated with CAN development in univariate analysis (
P < 0.01), the multivariate analysis did not show an association. There was no relation between CD14 gene polymorphism and CAN.
Conclusion: The MTHFR T677 allele is associated with the presence of CAN in kidney graft biopsies 12 months after transplantation.]]></abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>15369724</pmid><doi>10.1016/j.clinbiochem.2004.05.022</doi><tpages>6</tpages></addata></record> |
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| ispartof | Clinical biochemistry, 2004-10, Vol.37 (10), p.919-924 |
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| subjects | Alleles CD14 Chronic allograft nephropathy Chronic Disease Female Gene polymorphism Genetic Predisposition to Disease Graft vs Host Disease - enzymology Graft vs Host Disease - genetics Humans IL-6 Interleukin-6 - genetics Kidney Diseases - enzymology Kidney Diseases - genetics Kidney Transplantation Lipopolysaccharide Receptors - genetics Male Methylenetetrahydrofolate Reductase (NADPH2) - genetics Middle Aged MTHFR Polymorphism, Genetic Proteinuria Transplantation, Homologous |
| title | Association of methylenetetrahydrofolate reductase T677 allele with early development of chronic allograft nephropathy |
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