A Facile Method for High-throughput Co-expression of Protein Pairs

We developed a method to co-express protein pairs from collections of otherwise identical Escherichia coli plasmids expressing different ORFs by incorporating a 61-nucleotide sequence (LINK) into the plasmid to allow generation of tandem plasmids. Tandem plasmids are formed in a ligation-independent...

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Veröffentlicht in:	Molecular & cellular proteomics 2004-09, Vol.3 (9), p.934-938
Hauptverfasser:	Alexandrov, Andrei, Vignali, Marissa, LaCount, Douglas J, Quartley, Erin, de Vries, Christina, De Rosa, Daniela, Babulski, Julie, Mitchell, Sarah F, Schoenfeld, Lori W, Fields, Stanley, Hol, Wim G, Dumont, Mark E, Phizicky, Eric M, Grayhack, Elizabeth J
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence DNA, Complementary - genetics Escherichia coli Escherichia coli - genetics Gene Expression Genetic Vectors Open Reading Frames Plasmids - genetics Proteomics - methods Protozoan Proteins - biosynthesis Protozoan Proteins - genetics Recombinant Proteins - biosynthesis Recombinant Proteins - genetics Saccharomyces cerevisiae Proteins - biosynthesis Saccharomyces cerevisiae Proteins - genetics
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