A Facile Method for High-throughput Co-expression of Protein Pairs
We developed a method to co-express protein pairs from collections of otherwise identical Escherichia coli plasmids expressing different ORFs by incorporating a 61-nucleotide sequence (LINK) into the plasmid to allow generation of tandem plasmids. Tandem plasmids are formed in a ligation-independent...
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| Veröffentlicht in: | Molecular & cellular proteomics 2004-09, Vol.3 (9), p.934-938 |
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| container_title | Molecular & cellular proteomics |
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| creator | Alexandrov, Andrei Vignali, Marissa LaCount, Douglas J Quartley, Erin de Vries, Christina De Rosa, Daniela Babulski, Julie Mitchell, Sarah F Schoenfeld, Lori W Fields, Stanley Hol, Wim G Dumont, Mark E Phizicky, Eric M Grayhack, Elizabeth J |
| description | We developed a method to co-express protein pairs from collections of otherwise identical Escherichia coli plasmids expressing different ORFs by incorporating a 61-nucleotide sequence (LINK) into the plasmid to allow generation
of tandem plasmids. Tandem plasmids are formed in a ligation-independent manner, propagate efficiently, and produce protein
pairs in high quantities. This greatly facilitates co-expression for structural genomics projects that produce thousands of
clones bearing identical origins and antibiotic markers. |
| doi_str_mv | 10.1074/mcp.T400008-MCP200 |
| format | Article |
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of tandem plasmids. Tandem plasmids are formed in a ligation-independent manner, propagate efficiently, and produce protein
pairs in high quantities. This greatly facilitates co-expression for structural genomics projects that produce thousands of
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of tandem plasmids. Tandem plasmids are formed in a ligation-independent manner, propagate efficiently, and produce protein
pairs in high quantities. This greatly facilitates co-expression for structural genomics projects that produce thousands of
clones bearing identical origins and antibiotic markers.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>DNA, Complementary - genetics</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Gene Expression</subject><subject>Genetic Vectors</subject><subject>Open Reading Frames</subject><subject>Plasmids - genetics</subject><subject>Proteomics - methods</subject><subject>Protozoan Proteins - biosynthesis</subject><subject>Protozoan Proteins - genetics</subject><subject>Recombinant Proteins - biosynthesis</subject><subject>Recombinant Proteins - genetics</subject><subject>Saccharomyces cerevisiae Proteins - biosynthesis</subject><subject>Saccharomyces cerevisiae Proteins - genetics</subject><issn>1535-9476</issn><issn>1535-9484</issn><issn>1535-9484</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkLFOwzAURS0EolD4AQbwxJZiO46djCWiFKkVHcpsJY7dGCV1sBMBf4-rVDDylvuGc-9wALjBaIYRpw-t7GZbisKl0TrfEIROwAVO4iTKaEpPf3_OJuDS-3eECMI8OQcTnBCKUhJfgMc5XBTSNAquVV_bCmrr4NLs6qivnR12dTf0MLeR-uqc8t7YPbQabpztldnDTWGcvwJnumi8uj7mFLwtnrb5Mlq9Pr_k81UkKaN9JHlVFSzlsaxoRRgvlWZlohlSmS4pKTBhupQyKRTmPE0kYgeGskprkrEKx1NwP-52zn4MyveiNV6qpin2yg5esDCeIvI_iDPOgwcWQDKC0lnvndKic6Yt3LfASBwUi6BYHBWLUXEo3R7Xh7JV1V_l6DQAdyNQB42fxilRGitr1YpYZCKLafwDZKeCpA</recordid><startdate>20040901</startdate><enddate>20040901</enddate><creator>Alexandrov, Andrei</creator><creator>Vignali, Marissa</creator><creator>LaCount, Douglas J</creator><creator>Quartley, Erin</creator><creator>de Vries, Christina</creator><creator>De Rosa, Daniela</creator><creator>Babulski, Julie</creator><creator>Mitchell, Sarah F</creator><creator>Schoenfeld, Lori W</creator><creator>Fields, Stanley</creator><creator>Hol, Wim G</creator><creator>Dumont, Mark E</creator><creator>Phizicky, Eric M</creator><creator>Grayhack, Elizabeth J</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20040901</creationdate><title>A Facile Method for High-throughput Co-expression of Protein Pairs</title><author>Alexandrov, Andrei ; 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pairs in high quantities. This greatly facilitates co-expression for structural genomics projects that produce thousands of
clones bearing identical origins and antibiotic markers.</abstract><cop>United States</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>15240823</pmid><doi>10.1074/mcp.T400008-MCP200</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Animals Base Sequence DNA, Complementary - genetics Escherichia coli Escherichia coli - genetics Gene Expression Genetic Vectors Open Reading Frames Plasmids - genetics Proteomics - methods Protozoan Proteins - biosynthesis Protozoan Proteins - genetics Recombinant Proteins - biosynthesis Recombinant Proteins - genetics Saccharomyces cerevisiae Proteins - biosynthesis Saccharomyces cerevisiae Proteins - genetics |
| title | A Facile Method for High-throughput Co-expression of Protein Pairs |
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