Cell mediated immune response elicited in mice after immunization with the P64k meningococcal protein: epitope mapping
The P64k protein of Neisseria meningitidis has been reported as an immunological carrier for weak immunogens. This investigation was aimed at characterizing the T-cell response produced in primed mice and at identifying T helper cell epitopes within this molecule. BALB/c mice subcutaneously immunize...
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| Veröffentlicht in: | FEMS immunology and medical microbiology 2004-10, Vol.42 (2), p.233-239 |
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| creator | González, Sonia Nazábal, Consuelo Rao, Kanury V.S. Reyes, Osvaldo Garay, Hilda E. Caballero, Evelin Alvarez-Obregón, Julio C. Sardiñas, Gretel Silva, Ricardo |
| description | The P64k protein of
Neisseria meningitidis has been reported as an immunological carrier for weak immunogens. This investigation was aimed at characterizing the T-cell response produced in primed mice and at identifying T helper cell epitopes within this molecule. BALB/c mice subcutaneously immunized with the recombinant antigen provided inguinal lymph node cells (LNC) that proliferated in the presence of P64k in a dose-dependent manner. Proliferating cells secreted IL-4 while the concentration of IL-12 remained unaltered in the culture supernatant. By testing a panel of 59 overlapping synthetic peptides spanning the entire sequence of the antigen a T-cell determinant was localized. Prime-boost and lymphoproliferation experiments, conducted with highly purified synthetic peptides, confirmed that the segment including amino acids 470–485 comprises a T-cell epitope within the P64k molecule. |
| doi_str_mv | 10.1016/j.femsim.2004.05.007 |
| format | Article |
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Neisseria meningitidis has been reported as an immunological carrier for weak immunogens. This investigation was aimed at characterizing the T-cell response produced in primed mice and at identifying T helper cell epitopes within this molecule. BALB/c mice subcutaneously immunized with the recombinant antigen provided inguinal lymph node cells (LNC) that proliferated in the presence of P64k in a dose-dependent manner. Proliferating cells secreted IL-4 while the concentration of IL-12 remained unaltered in the culture supernatant. By testing a panel of 59 overlapping synthetic peptides spanning the entire sequence of the antigen a T-cell determinant was localized. Prime-boost and lymphoproliferation experiments, conducted with highly purified synthetic peptides, confirmed that the segment including amino acids 470–485 comprises a T-cell epitope within the P64k molecule.</description><identifier>ISSN: 0928-8244</identifier><identifier>EISSN: 1574-695X</identifier><identifier>EISSN: 2049-632X</identifier><identifier>DOI: 10.1016/j.femsim.2004.05.007</identifier><identifier>PMID: 15364109</identifier><language>eng</language><publisher>Oxford, UK: Elsevier B.V</publisher><subject>Amino Acid Sequence ; Amino acids ; Animals ; Antigens ; Applied microbiology ; Bacterial Outer Membrane Proteins - chemistry ; Bacterial Outer Membrane Proteins - genetics ; Bacterial Outer Membrane Proteins - immunology ; Bacteriology ; Biological and medical sciences ; Cell culture ; Epitope Mapping ; Epitopes, T-Lymphocyte - immunology ; Female ; Fundamental and applied biological sciences. Psychology ; Helper cells ; Helper T-cell epitope ; Immune response ; Immune system ; Immunization ; Immunology ; Interleukin 12 ; Interleukin 4 ; Lymph nodes ; Lymph Nodes - chemistry ; Lymph Nodes - immunology ; Lymphocyte Activation ; Lymphocytes T ; Meningococcal Infections - prevention & control ; Meningococcal Vaccines - administration & dosage ; Meningococcal Vaccines - immunology ; Meningococci ; Mice ; Mice, Inbred BALB C ; Microbiology ; Miscellaneous ; Molecular Sequence Data ; Neisseria meningitidis ; Neisseria meningitidis - immunology ; P64k ; Peptide mapping ; Peptides ; Peptides - chemistry ; Peptides - immunology ; Proteins ; Recombinant Proteins - immunology ; Synthetic peptides ; T-cell response ; Th1 Cells - immunology ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><ispartof>FEMS immunology and medical microbiology, 2004-10, Vol.42 (2), p.233-239</ispartof><rights>2004 Federation of European Microbiological Societies</rights><rights>2004 Federation of European Microbiological Societies. 2004</rights><rights>2004 INIST-CNRS</rights><rights>2004 Federation of European Microbiological Societies.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5823-55d060135d1f9120346ee95815375b82de8e66bf1e8261289a1abb9608916c783</citedby><cites>FETCH-LOGICAL-c5823-55d060135d1f9120346ee95815375b82de8e66bf1e8261289a1abb9608916c783</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2Fj.femsim.2004.05.007$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1016%2Fj.femsim.2004.05.007$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16119331$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15364109$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>González, Sonia</creatorcontrib><creatorcontrib>Nazábal, Consuelo</creatorcontrib><creatorcontrib>Rao, Kanury V.S.</creatorcontrib><creatorcontrib>Reyes, Osvaldo</creatorcontrib><creatorcontrib>Garay, Hilda E.</creatorcontrib><creatorcontrib>Caballero, Evelin</creatorcontrib><creatorcontrib>Alvarez-Obregón, Julio C.</creatorcontrib><creatorcontrib>Sardiñas, Gretel</creatorcontrib><creatorcontrib>Silva, Ricardo</creatorcontrib><title>Cell mediated immune response elicited in mice after immunization with the P64k meningococcal protein: epitope mapping</title><title>FEMS immunology and medical microbiology</title><addtitle>FEMS Immunol Med Microbiol</addtitle><description>The P64k protein of
Neisseria meningitidis has been reported as an immunological carrier for weak immunogens. This investigation was aimed at characterizing the T-cell response produced in primed mice and at identifying T helper cell epitopes within this molecule. BALB/c mice subcutaneously immunized with the recombinant antigen provided inguinal lymph node cells (LNC) that proliferated in the presence of P64k in a dose-dependent manner. Proliferating cells secreted IL-4 while the concentration of IL-12 remained unaltered in the culture supernatant. By testing a panel of 59 overlapping synthetic peptides spanning the entire sequence of the antigen a T-cell determinant was localized. Prime-boost and lymphoproliferation experiments, conducted with highly purified synthetic peptides, confirmed that the segment including amino acids 470–485 comprises a T-cell epitope within the P64k molecule.</description><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Animals</subject><subject>Antigens</subject><subject>Applied microbiology</subject><subject>Bacterial Outer Membrane Proteins - chemistry</subject><subject>Bacterial Outer Membrane Proteins - genetics</subject><subject>Bacterial Outer Membrane Proteins - immunology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Cell culture</subject><subject>Epitope Mapping</subject><subject>Epitopes, T-Lymphocyte - immunology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Helper cells</subject><subject>Helper T-cell epitope</subject><subject>Immune response</subject><subject>Immune system</subject><subject>Immunization</subject><subject>Immunology</subject><subject>Interleukin 12</subject><subject>Interleukin 4</subject><subject>Lymph nodes</subject><subject>Lymph Nodes - chemistry</subject><subject>Lymph Nodes - immunology</subject><subject>Lymphocyte Activation</subject><subject>Lymphocytes T</subject><subject>Meningococcal Infections - prevention & control</subject><subject>Meningococcal Vaccines - administration & dosage</subject><subject>Meningococcal Vaccines - immunology</subject><subject>Meningococci</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Molecular Sequence Data</subject><subject>Neisseria meningitidis</subject><subject>Neisseria meningitidis - immunology</subject><subject>P64k</subject><subject>Peptide mapping</subject><subject>Peptides</subject><subject>Peptides - chemistry</subject><subject>Peptides - immunology</subject><subject>Proteins</subject><subject>Recombinant Proteins - immunology</subject><subject>Synthetic peptides</subject><subject>T-cell response</subject><subject>Th1 Cells - immunology</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><issn>0928-8244</issn><issn>1574-695X</issn><issn>2049-632X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkk9rFTEUxYMo9ln9BiIB0d1M82eSSVwI8rBaqOhCwV3Iy9yxec4k02SmpX56U-dBwYV2lcX9nZNzc4LQc0pqSqg82dc9jNmPNSOkqYmoCWkfoA0VbVNJLb4_RBuimaoUa5oj9CTnPSmgJuQxOqKCy4YSvUFXWxgGPELn7Qwd9uO4BMAJ8hRDBgyDd_7PIODRO8C2nyGtmP9lZx8DvvbzBZ4vAH-Rzc9iFXz4EV10zg54SnEGH95gmPwcJ8CjnaYyf4oe9XbI8OxwHqNvp--_bj9W558_nG3fnVdOKMYrIToiCeWio72mjPBGAmihSv5W7BTrQIGUu56CYpIypS21u52WRGkqXav4MXq9-pYglwvk2Yw-u7KyDRCXbKRULWeN_C9I21a2nOgCvvwL3MclhbKEYZy0VImGs0I1K-VSzDlBb6bkR5tuDCXmtj6zN2t95rY-Q4Qp9RXZi4P5siud3IkOfRXg1QGwubxvn2xwPt9xklLNOS2cWrlrP8DNvS43p2efGOdFerJK4zLdN_XbVQGlySsPyWTnIbjyqRK42XTR_9vgN7Ex2nE</recordid><startdate>200410</startdate><enddate>200410</enddate><creator>González, Sonia</creator><creator>Nazábal, Consuelo</creator><creator>Rao, Kanury V.S.</creator><creator>Reyes, Osvaldo</creator><creator>Garay, Hilda E.</creator><creator>Caballero, Evelin</creator><creator>Alvarez-Obregón, Julio C.</creator><creator>Sardiñas, Gretel</creator><creator>Silva, Ricardo</creator><general>Elsevier B.V</general><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>7QL</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>200410</creationdate><title>Cell mediated immune response elicited in mice after immunization with the P64k meningococcal protein: epitope mapping</title><author>González, Sonia ; Nazábal, Consuelo ; Rao, Kanury V.S. ; Reyes, Osvaldo ; Garay, Hilda E. ; Caballero, Evelin ; Alvarez-Obregón, Julio C. ; Sardiñas, Gretel ; Silva, Ricardo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5823-55d060135d1f9120346ee95815375b82de8e66bf1e8261289a1abb9608916c783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>Animals</topic><topic>Antigens</topic><topic>Applied microbiology</topic><topic>Bacterial Outer Membrane Proteins - chemistry</topic><topic>Bacterial Outer Membrane Proteins - genetics</topic><topic>Bacterial Outer Membrane Proteins - immunology</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Cell culture</topic><topic>Epitope Mapping</topic><topic>Epitopes, T-Lymphocyte - immunology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Helper cells</topic><topic>Helper T-cell epitope</topic><topic>Immune response</topic><topic>Immune system</topic><topic>Immunization</topic><topic>Immunology</topic><topic>Interleukin 12</topic><topic>Interleukin 4</topic><topic>Lymph nodes</topic><topic>Lymph Nodes - chemistry</topic><topic>Lymph Nodes - immunology</topic><topic>Lymphocyte Activation</topic><topic>Lymphocytes T</topic><topic>Meningococcal Infections - prevention & control</topic><topic>Meningococcal Vaccines - administration & dosage</topic><topic>Meningococcal Vaccines - immunology</topic><topic>Meningococci</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Molecular Sequence Data</topic><topic>Neisseria meningitidis</topic><topic>Neisseria meningitidis - immunology</topic><topic>P64k</topic><topic>Peptide mapping</topic><topic>Peptides</topic><topic>Peptides - chemistry</topic><topic>Peptides - immunology</topic><topic>Proteins</topic><topic>Recombinant Proteins - immunology</topic><topic>Synthetic peptides</topic><topic>T-cell response</topic><topic>Th1 Cells - immunology</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>González, Sonia</creatorcontrib><creatorcontrib>Nazábal, Consuelo</creatorcontrib><creatorcontrib>Rao, Kanury V.S.</creatorcontrib><creatorcontrib>Reyes, Osvaldo</creatorcontrib><creatorcontrib>Garay, Hilda E.</creatorcontrib><creatorcontrib>Caballero, Evelin</creatorcontrib><creatorcontrib>Alvarez-Obregón, Julio C.</creatorcontrib><creatorcontrib>Sardiñas, Gretel</creatorcontrib><creatorcontrib>Silva, Ricardo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS immunology and medical microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>González, Sonia</au><au>Nazábal, Consuelo</au><au>Rao, Kanury V.S.</au><au>Reyes, Osvaldo</au><au>Garay, Hilda E.</au><au>Caballero, Evelin</au><au>Alvarez-Obregón, Julio C.</au><au>Sardiñas, Gretel</au><au>Silva, Ricardo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cell mediated immune response elicited in mice after immunization with the P64k meningococcal protein: epitope mapping</atitle><jtitle>FEMS immunology and medical microbiology</jtitle><addtitle>FEMS Immunol Med Microbiol</addtitle><date>2004-10</date><risdate>2004</risdate><volume>42</volume><issue>2</issue><spage>233</spage><epage>239</epage><pages>233-239</pages><issn>0928-8244</issn><eissn>1574-695X</eissn><eissn>2049-632X</eissn><abstract>The P64k protein of
Neisseria meningitidis has been reported as an immunological carrier for weak immunogens. This investigation was aimed at characterizing the T-cell response produced in primed mice and at identifying T helper cell epitopes within this molecule. BALB/c mice subcutaneously immunized with the recombinant antigen provided inguinal lymph node cells (LNC) that proliferated in the presence of P64k in a dose-dependent manner. Proliferating cells secreted IL-4 while the concentration of IL-12 remained unaltered in the culture supernatant. By testing a panel of 59 overlapping synthetic peptides spanning the entire sequence of the antigen a T-cell determinant was localized. Prime-boost and lymphoproliferation experiments, conducted with highly purified synthetic peptides, confirmed that the segment including amino acids 470–485 comprises a T-cell epitope within the P64k molecule.</abstract><cop>Oxford, UK</cop><pub>Elsevier B.V</pub><pmid>15364109</pmid><doi>10.1016/j.femsim.2004.05.007</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| issn | 0928-8244 1574-695X 2049-632X |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Wiley Online Library Journals Frontfile Complete; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Amino acids Animals Antigens Applied microbiology Bacterial Outer Membrane Proteins - chemistry Bacterial Outer Membrane Proteins - genetics Bacterial Outer Membrane Proteins - immunology Bacteriology Biological and medical sciences Cell culture Epitope Mapping Epitopes, T-Lymphocyte - immunology Female Fundamental and applied biological sciences. Psychology Helper cells Helper T-cell epitope Immune response Immune system Immunization Immunology Interleukin 12 Interleukin 4 Lymph nodes Lymph Nodes - chemistry Lymph Nodes - immunology Lymphocyte Activation Lymphocytes T Meningococcal Infections - prevention & control Meningococcal Vaccines - administration & dosage Meningococcal Vaccines - immunology Meningococci Mice Mice, Inbred BALB C Microbiology Miscellaneous Molecular Sequence Data Neisseria meningitidis Neisseria meningitidis - immunology P64k Peptide mapping Peptides Peptides - chemistry Peptides - immunology Proteins Recombinant Proteins - immunology Synthetic peptides T-cell response Th1 Cells - immunology Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) |
| title | Cell mediated immune response elicited in mice after immunization with the P64k meningococcal protein: epitope mapping |
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