Isolation and cultivation of stem cells from adult mouse testes
The successful isolation and cultivation of spermatogonial stem cells (SSCs) as well as induction of SSCs into pluripotent stem cells will allow us to study their biological characteristics and their applications in therapeutic approaches. Here we provide step-by-step procedures on the basis of prev...
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| Veröffentlicht in: | Nature protocols 2009-02, Vol.4 (2), p.143-154 |
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| creator | Guan, Kaomei Wolf, Frieder Becker, Alexander Engel, Wolfgang Nayernia, Karim Hasenfuss, Gerd |
| description | The successful isolation and cultivation of spermatogonial stem cells (SSCs) as well as induction of SSCs into pluripotent stem cells will allow us to study their biological characteristics and their applications in therapeutic approaches. Here we provide step-by-step procedures on the basis of previous work in our laboratory for: the isolation of testicular cells from adolescent mice by a modified enzymatic procedure; the enrichment of undifferentiated spermatogonia by laminin selection or genetic selection using Stra8-EGFP (enhanced green fluorescent protein) transgenic mice; the cultivation and conversion of undifferentiated spermatogonia into embryonic stem-like cells, so-called multipotent adult germline stem cells (maGSCs); and characterization of these cells. Normally, it will take about 16 weeks to obtain stable maGSC lines starting from the isolation of testicular cells. |
| doi_str_mv | 10.1038/nprot.2008.242 |
| format | Article |
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Academic</collection><jtitle>Nature protocols</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guan, Kaomei</au><au>Wolf, Frieder</au><au>Becker, Alexander</au><au>Engel, Wolfgang</au><au>Nayernia, Karim</au><au>Hasenfuss, Gerd</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and cultivation of stem cells from adult mouse testes</atitle><jtitle>Nature protocols</jtitle><stitle>Nat Protoc</stitle><addtitle>Nat Protoc</addtitle><date>2009-02-01</date><risdate>2009</risdate><volume>4</volume><issue>2</issue><spage>143</spage><epage>154</epage><pages>143-154</pages><issn>1754-2189</issn><eissn>1750-2799</eissn><abstract>The successful isolation and cultivation of spermatogonial stem cells (SSCs) as well as induction of SSCs into pluripotent stem cells will allow us to study their biological characteristics and their applications in therapeutic approaches. Here we provide step-by-step procedures on the basis of previous work in our laboratory for: the isolation of testicular cells from adolescent mice by a modified enzymatic procedure; the enrichment of undifferentiated spermatogonia by laminin selection or genetic selection using Stra8-EGFP (enhanced green fluorescent protein) transgenic mice; the cultivation and conversion of undifferentiated spermatogonia into embryonic stem-like cells, so-called multipotent adult germline stem cells (maGSCs); and characterization of these cells. Normally, it will take about 16 weeks to obtain stable maGSC lines starting from the isolation of testicular cells.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>19180086</pmid><doi>10.1038/nprot.2008.242</doi><tpages>12</tpages></addata></record> |
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| subjects | Adolescents Analytical Chemistry Animals Biological Techniques Biomedical and Life Sciences Cell culture Cell Culture Techniques - methods Computational Biology/Bioinformatics Cultivation Cytokines Green Fluorescent Proteins Growth factors Life Sciences Male Methods Mice Mice, Transgenic Microarrays Morphology Organic Chemistry Physiological aspects Properties Proteins protocol Spermatogenesis Spermatogonia - cytology Stem cells Stem Cells - cytology Testes Testis - cytology Transgenic animals |
| title | Isolation and cultivation of stem cells from adult mouse testes |
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