Molecular typing of Yersinia frederiksenii strains by means of 16s rDNA and gyrB genes sequence analyses
Abstract The phenospecies Yersinia frederiksenii is known to comprise three genospecies, indistinguishable on the basis of phenotypic characteristics. In previous works, 13 strains, identified biochemically as Y. frederiksenii, were characterized using Multilocus enzyme electrophoresis and Ribotypin...
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| Veröffentlicht in: | FEMS microbiology letters 2004-09, Vol.238 (2), p.423-428 |
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| creator | Demarta, A. De Respinis, S. Dolina, M. Peduzzi, R. |
| description | Abstract
The phenospecies Yersinia frederiksenii is known to comprise three genospecies, indistinguishable on the basis of phenotypic characteristics. In previous works, 13 strains, identified biochemically as Y. frederiksenii, were characterized using Multilocus enzyme electrophoresis and Ribotyping. In order to elucidate the phylogenetic position of these strains we performed their molecular typing by means of 16S rDNA and gyrB sequences analyses. Results demonstrated that gyrB is a better phylogenetic marker than 16S rDNA. The classification achieved by gyrB sequences analyses was in agreement with results obtained with more laborious methods. Moreover, a good phylogenetic identification could be reached also with partial gyrB sequences of only 350 bp. |
| doi_str_mv | 10.1111/j.1574-6968.2004.tb09785.x |
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The phenospecies Yersinia frederiksenii is known to comprise three genospecies, indistinguishable on the basis of phenotypic characteristics. In previous works, 13 strains, identified biochemically as Y. frederiksenii, were characterized using Multilocus enzyme electrophoresis and Ribotyping. In order to elucidate the phylogenetic position of these strains we performed their molecular typing by means of 16S rDNA and gyrB sequences analyses. Results demonstrated that gyrB is a better phylogenetic marker than 16S rDNA. The classification achieved by gyrB sequences analyses was in agreement with results obtained with more laborious methods. Moreover, a good phylogenetic identification could be reached also with partial gyrB sequences of only 350 bp.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.2004.tb09785.x</identifier><identifier>PMID: 15358429</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>16S rDNA sequences ; Bacterial Typing Techniques ; Bacteriology ; Biological and medical sciences ; DNA Gyrase ; Electrophoresis ; Fundamental and applied biological sciences. Psychology ; Genetics ; Genospecies ; gyrB sequences ; Identification methods ; Microbiology ; Molecular Sequence Data ; Molecular typing ; Phylogenetic identification ; Phylogenetics ; Phylogeny ; Ribotyping ; RNA, Bacterial - analysis ; RNA, Bacterial - genetics ; RNA, Ribosomal, 16S - analysis ; RNA, Ribosomal, 16S - genetics ; rRNA 16S ; Sequence Analysis, DNA ; Systematics ; Yersinia - classification ; Yersinia - genetics ; Yersinia - isolation & purification ; Yersinia frederiksenii</subject><ispartof>FEMS microbiology letters, 2004-09, Vol.238 (2), p.423-428</ispartof><rights>2004 Federation of European Microbiological Societies 2004</rights><rights>2005 INIST-CNRS</rights><rights>2004 Federation of European Microbiological Societies</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c2643-eedf4144f899d29034d4845b322caf15ab9f1e585da9bfee6d01798c017b73113</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-6968.2004.tb09785.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-6968.2004.tb09785.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16086577$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15358429$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Demarta, A.</creatorcontrib><creatorcontrib>De Respinis, S.</creatorcontrib><creatorcontrib>Dolina, M.</creatorcontrib><creatorcontrib>Peduzzi, R.</creatorcontrib><title>Molecular typing of Yersinia frederiksenii strains by means of 16s rDNA and gyrB genes sequence analyses</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>Abstract
The phenospecies Yersinia frederiksenii is known to comprise three genospecies, indistinguishable on the basis of phenotypic characteristics. In previous works, 13 strains, identified biochemically as Y. frederiksenii, were characterized using Multilocus enzyme electrophoresis and Ribotyping. In order to elucidate the phylogenetic position of these strains we performed their molecular typing by means of 16S rDNA and gyrB sequences analyses. Results demonstrated that gyrB is a better phylogenetic marker than 16S rDNA. The classification achieved by gyrB sequences analyses was in agreement with results obtained with more laborious methods. Moreover, a good phylogenetic identification could be reached also with partial gyrB sequences of only 350 bp.</description><subject>16S rDNA sequences</subject><subject>Bacterial Typing Techniques</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>DNA Gyrase</subject><subject>Electrophoresis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetics</subject><subject>Genospecies</subject><subject>gyrB sequences</subject><subject>Identification methods</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Molecular typing</subject><subject>Phylogenetic identification</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Ribotyping</subject><subject>RNA, Bacterial - analysis</subject><subject>RNA, Bacterial - genetics</subject><subject>RNA, Ribosomal, 16S - analysis</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>rRNA 16S</subject><subject>Sequence Analysis, DNA</subject><subject>Systematics</subject><subject>Yersinia - classification</subject><subject>Yersinia - genetics</subject><subject>Yersinia - isolation & purification</subject><subject>Yersinia frederiksenii</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqVkU2P0zAQhi0EYsvCX0AWCG4J_o7DAWlZWEDqwgUOnCwnGReXNCmeRmz-Pa4asRKCAz7YI8_zjl_PEPKEs5Ln9WJbcl2pwtTGloIxVR4aVldWlzd3yOp36i5ZMVnZgufcGXmAuGWZFczcJ2dcS22VqFfk2_XYQzv1PtHDvI_Dho6BfoWEcYiehgQdpPgdYYiR4iH5OCBtZroDn4OMcoM0vfl4Qf3Q0c2cXtMNDIAU4ccEQwv53vczAj4k94LvER4t5zn5cvX28-X7Yv3p3YfLi3XRCqNkAdAFxZUKtq47UTOpOmWVbqQQrQ9c-6YOHLTVna-bAGA6xqvatnlvKsm5PCfPT3X3acwW8OB2EVvoez_AOKEzxhrFpcjg0z_A7Til7BadkKzixkirM_XyRLVpREwQ3D7FnU-z48wdp-G27thyd2y5O07DLdNwN1n8eHlianbQ3UqX9mfg2QJ4bH0fkh_aiLecYdboqsrcqxP3M_Yw_4cFd3W9VkLmAvpUYJz2_5AXf_vBL1GOtnE</recordid><startdate>200409</startdate><enddate>200409</enddate><creator>Demarta, A.</creator><creator>De Respinis, S.</creator><creator>Dolina, M.</creator><creator>Peduzzi, R.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200409</creationdate><title>Molecular typing of Yersinia frederiksenii strains by means of 16s rDNA and gyrB genes sequence analyses</title><author>Demarta, A. ; De Respinis, S. ; Dolina, M. ; Peduzzi, R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2643-eedf4144f899d29034d4845b322caf15ab9f1e585da9bfee6d01798c017b73113</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>16S rDNA sequences</topic><topic>Bacterial Typing Techniques</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>DNA Gyrase</topic><topic>Electrophoresis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetics</topic><topic>Genospecies</topic><topic>gyrB sequences</topic><topic>Identification methods</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Molecular typing</topic><topic>Phylogenetic identification</topic><topic>Phylogenetics</topic><topic>Phylogeny</topic><topic>Ribotyping</topic><topic>RNA, Bacterial - analysis</topic><topic>RNA, Bacterial - genetics</topic><topic>RNA, Ribosomal, 16S - analysis</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>rRNA 16S</topic><topic>Sequence Analysis, DNA</topic><topic>Systematics</topic><topic>Yersinia - classification</topic><topic>Yersinia - genetics</topic><topic>Yersinia - isolation & purification</topic><topic>Yersinia frederiksenii</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Demarta, A.</creatorcontrib><creatorcontrib>De Respinis, S.</creatorcontrib><creatorcontrib>Dolina, M.</creatorcontrib><creatorcontrib>Peduzzi, R.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Demarta, A.</au><au>De Respinis, S.</au><au>Dolina, M.</au><au>Peduzzi, R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular typing of Yersinia frederiksenii strains by means of 16s rDNA and gyrB genes sequence analyses</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2004-09</date><risdate>2004</risdate><volume>238</volume><issue>2</issue><spage>423</spage><epage>428</epage><pages>423-428</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>Abstract
The phenospecies Yersinia frederiksenii is known to comprise three genospecies, indistinguishable on the basis of phenotypic characteristics. In previous works, 13 strains, identified biochemically as Y. frederiksenii, were characterized using Multilocus enzyme electrophoresis and Ribotyping. In order to elucidate the phylogenetic position of these strains we performed their molecular typing by means of 16S rDNA and gyrB sequences analyses. Results demonstrated that gyrB is a better phylogenetic marker than 16S rDNA. The classification achieved by gyrB sequences analyses was in agreement with results obtained with more laborious methods. Moreover, a good phylogenetic identification could be reached also with partial gyrB sequences of only 350 bp.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>15358429</pmid><doi>10.1111/j.1574-6968.2004.tb09785.x</doi><tpages>6</tpages></addata></record> |
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| issn | 0378-1097 1574-6968 |
| language | eng |
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| source | MEDLINE; Wiley Journals; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | 16S rDNA sequences Bacterial Typing Techniques Bacteriology Biological and medical sciences DNA Gyrase Electrophoresis Fundamental and applied biological sciences. Psychology Genetics Genospecies gyrB sequences Identification methods Microbiology Molecular Sequence Data Molecular typing Phylogenetic identification Phylogenetics Phylogeny Ribotyping RNA, Bacterial - analysis RNA, Bacterial - genetics RNA, Ribosomal, 16S - analysis RNA, Ribosomal, 16S - genetics rRNA 16S Sequence Analysis, DNA Systematics Yersinia - classification Yersinia - genetics Yersinia - isolation & purification Yersinia frederiksenii |
| title | Molecular typing of Yersinia frederiksenii strains by means of 16s rDNA and gyrB genes sequence analyses |
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