Identification and partial characterisation of a new lagovirus in Australian wild rabbits
Abstract Rabbit Haemorrhagic Disease Virus (RHDV) is widely used in Australia to control feral rabbit populations. Before RHDV was released on the Australian continent in 1996, antibodies cross-reacting in RHDV specific ELISAs were found in Australian wild rabbits, leading to the hypothesis that a n...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 2009-02, Vol.384 (1), p.97-105 |
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| creator | Strive, T Wright, J.D Robinson, A.J |
| description | Abstract Rabbit Haemorrhagic Disease Virus (RHDV) is widely used in Australia to control feral rabbit populations. Before RHDV was released on the Australian continent in 1996, antibodies cross-reacting in RHDV specific ELISAs were found in Australian wild rabbits, leading to the hypothesis that a non-pathogenic calicivirus had been circulating in rabbit populations in Australia, potentially providing some level of cross-immunoprotection to RHDV infection. For the detection of this putative virus, a universal lagovirus PCR test was developed to screen a variety of different tissues of wild caught rabbits. We identified a new lagovirus in the intestinal tissues of three apparently healthy young wild rabbits. Quantitative Real Time PCR analysis revealed high concentrations of viral RNA in intestinal tissues and suggests a faecal-oral mode of transmission. Genome organisation and phylogenetic analysis following the sequencing of the entire viral genome revealed a new member of the genus Lagovirus within the family Caliciviridae. |
| doi_str_mv | 10.1016/j.virol.2008.11.004 |
| format | Article |
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Before RHDV was released on the Australian continent in 1996, antibodies cross-reacting in RHDV specific ELISAs were found in Australian wild rabbits, leading to the hypothesis that a non-pathogenic calicivirus had been circulating in rabbit populations in Australia, potentially providing some level of cross-immunoprotection to RHDV infection. For the detection of this putative virus, a universal lagovirus PCR test was developed to screen a variety of different tissues of wild caught rabbits. We identified a new lagovirus in the intestinal tissues of three apparently healthy young wild rabbits. Quantitative Real Time PCR analysis revealed high concentrations of viral RNA in intestinal tissues and suggests a faecal-oral mode of transmission. 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Before RHDV was released on the Australian continent in 1996, antibodies cross-reacting in RHDV specific ELISAs were found in Australian wild rabbits, leading to the hypothesis that a non-pathogenic calicivirus had been circulating in rabbit populations in Australia, potentially providing some level of cross-immunoprotection to RHDV infection. For the detection of this putative virus, a universal lagovirus PCR test was developed to screen a variety of different tissues of wild caught rabbits. We identified a new lagovirus in the intestinal tissues of three apparently healthy young wild rabbits. Quantitative Real Time PCR analysis revealed high concentrations of viral RNA in intestinal tissues and suggests a faecal-oral mode of transmission. Genome organisation and phylogenetic analysis following the sequencing of the entire viral genome revealed a new member of the genus Lagovirus within the family Caliciviridae.</description><subject>Animals</subject><subject>Animals, Wild - virology</subject><subject>Australia</subject><subject>Base Sequence</subject><subject>Blotting, Western</subject><subject>Bunyaviridae Infections - veterinary</subject><subject>Caliciviridae</subject><subject>Calicivirus</subject><subject>Conserved Sequence</subject><subject>DNA Primers</subject><subject>DNA, Viral - genetics</subject><subject>EBHSV</subject><subject>Enteric</subject><subject>Food Industry</subject><subject>Genome, Viral</subject><subject>Infectious Disease</subject><subject>Lagovirus - classification</subject><subject>Lagovirus - genetics</subject><subject>Lagovirus - isolation & purification</subject><subject>Meat - virology</subject><subject>Molecular Sequence Data</subject><subject>Polymerase Chain Reaction</subject><subject>Rabbits - virology</subject><subject>RHDV</subject><subject>Sequence Alignment</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1rVDEUhoModqz-AkGycnev5yT3KwuFUtQWCi7UhauQmw_NmMkdk9yW_nszzkDBTVchyfOeA89LyGuEFgGHd9v21qcltAxgahFbgO4J2SCIoQHe4VOyqS-sGSbGzsiLnLdQ7-MIz8kZCujE1LEN-XFtbCzeea2KXyJV0dC9SsWrQPUvlZQuNvl8_FwcVTTaOxrUz6UuXzP1kV6suSQVvIr0zgdDk5pnX_JL8sypkO2r03lOvn_6-O3yqrn58vn68uKm0V3HS8OFgBH70eqxM71xQsyMK1STdsPAsRfGgUZjxSwmxx0I65yb0alunFD3jJ-Tt8e5-7T8WW0ucueztiGoaJc1y2GYesEEPgoyYKLrgVeQH0GdlpyTdXKf_E6le4kgD-rlVv5TLw_qJaKsYmvqzWn8Ou-secicXFfg_RGw1catt0lm7W3U1vhkdZFm8Y8s-PBfXgcfa3Hht723ebusKVbREmVmEuTXQ_uH8mECGDkD_hen_Kvm</recordid><startdate>20090205</startdate><enddate>20090205</enddate><creator>Strive, T</creator><creator>Wright, J.D</creator><creator>Robinson, A.J</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20090205</creationdate><title>Identification and partial characterisation of a new lagovirus in Australian wild rabbits</title><author>Strive, T ; Wright, J.D ; Robinson, A.J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c443t-39907157ec74d5df99b23a1a8cf663159df0c1de9b98f3f09efffb1fa4781c523</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Animals, Wild - virology</topic><topic>Australia</topic><topic>Base Sequence</topic><topic>Blotting, Western</topic><topic>Bunyaviridae Infections - veterinary</topic><topic>Caliciviridae</topic><topic>Calicivirus</topic><topic>Conserved Sequence</topic><topic>DNA Primers</topic><topic>DNA, Viral - genetics</topic><topic>EBHSV</topic><topic>Enteric</topic><topic>Food Industry</topic><topic>Genome, Viral</topic><topic>Infectious Disease</topic><topic>Lagovirus - classification</topic><topic>Lagovirus - genetics</topic><topic>Lagovirus - isolation & purification</topic><topic>Meat - virology</topic><topic>Molecular Sequence Data</topic><topic>Polymerase Chain Reaction</topic><topic>Rabbits - virology</topic><topic>RHDV</topic><topic>Sequence Alignment</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Strive, T</creatorcontrib><creatorcontrib>Wright, J.D</creatorcontrib><creatorcontrib>Robinson, A.J</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Strive, T</au><au>Wright, J.D</au><au>Robinson, A.J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and partial characterisation of a new lagovirus in Australian wild rabbits</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>2009-02-05</date><risdate>2009</risdate><volume>384</volume><issue>1</issue><spage>97</spage><epage>105</epage><pages>97-105</pages><issn>0042-6822</issn><eissn>1096-0341</eissn><abstract>Abstract Rabbit Haemorrhagic Disease Virus (RHDV) is widely used in Australia to control feral rabbit populations. 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| ispartof | Virology (New York, N.Y.), 2009-02, Vol.384 (1), p.97-105 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Animals, Wild - virology Australia Base Sequence Blotting, Western Bunyaviridae Infections - veterinary Caliciviridae Calicivirus Conserved Sequence DNA Primers DNA, Viral - genetics EBHSV Enteric Food Industry Genome, Viral Infectious Disease Lagovirus - classification Lagovirus - genetics Lagovirus - isolation & purification Meat - virology Molecular Sequence Data Polymerase Chain Reaction Rabbits - virology RHDV Sequence Alignment |
| title | Identification and partial characterisation of a new lagovirus in Australian wild rabbits |
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