Enzyme immobilization on epoxy- and 1,1′-carbonyldiimidazole-activated methacrylate-based monoliths

Monolithic Convective Interaction Media (CIM) have been activated with epoxide and imidazole carbamate functionalities and used as supports for covalent immobilization of protein A, deoxyribonuclease I, and trypsin. The efficiency of immobilization for these proteins was determined from the amount o...

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Veröffentlicht in:	Journal of separation science 2004-07, Vol.27 (10-11), p.811-818
Hauptverfasser:	Benčina, Katja, Podgornik, Aleš, Štrancar, Aleš, Benčina, Mojca
Format:	Artikel
Sprache:	eng
Schlagworte:	1,1′‐Carbonyldiimidazole 1′-Carbonyldiimidazole Activated matrix Animals Arginine - analogs & derivatives Arginine - chemistry Calibration Cattle Chromatography, High Pressure Liquid CIM Deoxyribonuclease I - chemistry DNA - metabolism Dose-Response Relationship, Drug Enzyme Enzymes, Immobilized - chemistry Epoxide Epoxy Compounds - chemistry Hydrogen-Ion Concentration Hydrolysis Imidazoles - chemistry Immobilization Immunoglobulin G - chemistry Kinetics Ligands Methacrylates - chemistry Models, Chemical Monoliths Pancreas - metabolism Protein Staphylococcal Protein A - chemistry Time Factors Trypsin - chemistry
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