Premature processing of mouse mammary tumor virus Gag polyprotein impairs intracellular capsid assembly
Abstract Mouse mammary tumor virus (MMTV) is the prototypical member of the Betaretrovirus genus, but the processes of its morphogenesis are poorly characterized. In this report, we describe an unusual intracellular processing of MMTV Gag polyprotein in human 293T cells transiently expressing MMTV f...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 2009-02, Vol.384 (1), p.33-37 |
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| container_title | Virology (New York, N.Y.) |
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| creator | Zábranský, Aleš Hadravová, Romana Štokrová, Jitka Sakalian, Michael Pichová, Iva |
| description | Abstract Mouse mammary tumor virus (MMTV) is the prototypical member of the Betaretrovirus genus, but the processes of its morphogenesis are poorly characterized. In this report, we describe an unusual intracellular processing of MMTV Gag polyprotein in human 293T cells transiently expressing MMTV from heterologous promoter. The same specific cleavage products of the viral protease were seen for the wild type as well as for nonmyristylated mutant of MMTV Gag polyprotein completely defective in the particle release. Inactivation of the viral protease resulted in more stable Gag polyprotein and in accumulation of intracytoplasmic particles for nonmyristylated Gag. The intracellular processing of nonmyristylated MMTV Gag indicates that protease activation in betaretrovirus can occur independently of budding. |
| doi_str_mv | 10.1016/j.virol.2008.10.038 |
| format | Article |
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In this report, we describe an unusual intracellular processing of MMTV Gag polyprotein in human 293T cells transiently expressing MMTV from heterologous promoter. The same specific cleavage products of the viral protease were seen for the wild type as well as for nonmyristylated mutant of MMTV Gag polyprotein completely defective in the particle release. Inactivation of the viral protease resulted in more stable Gag polyprotein and in accumulation of intracytoplasmic particles for nonmyristylated Gag. The intracellular processing of nonmyristylated MMTV Gag indicates that protease activation in betaretrovirus can occur independently of budding.</description><identifier>ISSN: 0042-6822</identifier><identifier>EISSN: 1096-0341</identifier><identifier>DOI: 10.1016/j.virol.2008.10.038</identifier><identifier>PMID: 19046754</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Amino Acid Substitution ; Animals ; Assembly ; Betaretrovirus ; Dexamethasone - pharmacology ; Female ; Gag ; Gene Products, gag - genetics ; Gene Products, gag - metabolism ; Humans ; Infectious Disease ; Kinetics ; Mammary Glands, Animal - virology ; Mammary Neoplasms, Animal - virology ; Mammary Neoplasms, Experimental - virology ; Mammary Tumor Virus, Mouse - genetics ; Mammary Tumor Virus, Mouse - metabolism ; Mice ; MMTV ; Mouse mammary tumor virus ; Peptide Hydrolases - genetics ; Peptide Hydrolases - metabolism ; Promoter Regions, Genetic ; Proviruses - genetics ; Restriction Mapping ; T-Lymphocytes - drug effects ; T-Lymphocytes - virology ; Transfection</subject><ispartof>Virology (New York, N.Y.), 2009-02, Vol.384 (1), p.33-37</ispartof><rights>Elsevier Inc.</rights><rights>2008 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c488t-3f64bea0542f12688bf3ecb733d9ea568cbe9b82937d237800550d4ce021b2883</citedby><cites>FETCH-LOGICAL-c488t-3f64bea0542f12688bf3ecb733d9ea568cbe9b82937d237800550d4ce021b2883</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.virol.2008.10.038$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19046754$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zábranský, Aleš</creatorcontrib><creatorcontrib>Hadravová, Romana</creatorcontrib><creatorcontrib>Štokrová, Jitka</creatorcontrib><creatorcontrib>Sakalian, Michael</creatorcontrib><creatorcontrib>Pichová, Iva</creatorcontrib><title>Premature processing of mouse mammary tumor virus Gag polyprotein impairs intracellular capsid assembly</title><title>Virology (New York, N.Y.)</title><addtitle>Virology</addtitle><description>Abstract Mouse mammary tumor virus (MMTV) is the prototypical member of the Betaretrovirus genus, but the processes of its morphogenesis are poorly characterized. In this report, we describe an unusual intracellular processing of MMTV Gag polyprotein in human 293T cells transiently expressing MMTV from heterologous promoter. The same specific cleavage products of the viral protease were seen for the wild type as well as for nonmyristylated mutant of MMTV Gag polyprotein completely defective in the particle release. Inactivation of the viral protease resulted in more stable Gag polyprotein and in accumulation of intracytoplasmic particles for nonmyristylated Gag. The intracellular processing of nonmyristylated MMTV Gag indicates that protease activation in betaretrovirus can occur independently of budding.</description><subject>Amino Acid Substitution</subject><subject>Animals</subject><subject>Assembly</subject><subject>Betaretrovirus</subject><subject>Dexamethasone - pharmacology</subject><subject>Female</subject><subject>Gag</subject><subject>Gene Products, gag - genetics</subject><subject>Gene Products, gag - metabolism</subject><subject>Humans</subject><subject>Infectious Disease</subject><subject>Kinetics</subject><subject>Mammary Glands, Animal - virology</subject><subject>Mammary Neoplasms, Animal - virology</subject><subject>Mammary Neoplasms, Experimental - virology</subject><subject>Mammary Tumor Virus, Mouse - genetics</subject><subject>Mammary Tumor Virus, Mouse - metabolism</subject><subject>Mice</subject><subject>MMTV</subject><subject>Mouse mammary tumor virus</subject><subject>Peptide Hydrolases - genetics</subject><subject>Peptide Hydrolases - metabolism</subject><subject>Promoter Regions, Genetic</subject><subject>Proviruses - genetics</subject><subject>Restriction Mapping</subject><subject>T-Lymphocytes - drug effects</subject><subject>T-Lymphocytes - virology</subject><subject>Transfection</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUsGKFDEQDaK44-oXCJKTtx4rSXc6fVCQRVdhQUE9h3S6esiYdNqke2H-ftPOgOBlTyHFe6-q3itCXjPYM2Dy3XF_71L0ew6gSmUPQj0hOwadrEDU7CnZAdS8korzK_Ii5yOUf9vCc3LFOqhl29Q7cvieMJhlTUjnFC3m7KYDjSMNcc1IgwnBpBNd1hATLf3WTG_Ngc7Rnwp-QTdRF2bjUqZuWpKx6P3qTaLWzNkN1OSMofenl-TZaHzGV5f3mvz6_OnnzZfq7tvt15uPd5WtlVoqMcq6RwNNzUfGpVL9KND2rRBDh6aRyvbY9Yp3oh24aBVA08BQWwTOeq6UuCZvz7pluj8r5kUHl7ehzIRlIy2laqTg8lEgB950kokCFGegTTHnhKOek9tM0Qz0FoQ-6r9B6C2IrViCKKw3F_m1Dzj841ycL4D3ZwAWN-4dJp2tw8ni4BLaRQ_RPdLgw398693krPG_8YT5GNc0FaM105lr0D-2W9hOAYpnbVEVD5x_sWQ</recordid><startdate>20090205</startdate><enddate>20090205</enddate><creator>Zábranský, Aleš</creator><creator>Hadravová, Romana</creator><creator>Štokrová, Jitka</creator><creator>Sakalian, Michael</creator><creator>Pichová, Iva</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TO</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20090205</creationdate><title>Premature processing of mouse mammary tumor virus Gag polyprotein impairs intracellular capsid assembly</title><author>Zábranský, Aleš ; Hadravová, Romana ; Štokrová, Jitka ; Sakalian, Michael ; Pichová, Iva</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c488t-3f64bea0542f12688bf3ecb733d9ea568cbe9b82937d237800550d4ce021b2883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Amino Acid Substitution</topic><topic>Animals</topic><topic>Assembly</topic><topic>Betaretrovirus</topic><topic>Dexamethasone - pharmacology</topic><topic>Female</topic><topic>Gag</topic><topic>Gene Products, gag - genetics</topic><topic>Gene Products, gag - metabolism</topic><topic>Humans</topic><topic>Infectious Disease</topic><topic>Kinetics</topic><topic>Mammary Glands, Animal - virology</topic><topic>Mammary Neoplasms, Animal - virology</topic><topic>Mammary Neoplasms, Experimental - virology</topic><topic>Mammary Tumor Virus, Mouse - genetics</topic><topic>Mammary Tumor Virus, Mouse - metabolism</topic><topic>Mice</topic><topic>MMTV</topic><topic>Mouse mammary tumor virus</topic><topic>Peptide Hydrolases - genetics</topic><topic>Peptide Hydrolases - metabolism</topic><topic>Promoter Regions, Genetic</topic><topic>Proviruses - genetics</topic><topic>Restriction Mapping</topic><topic>T-Lymphocytes - drug effects</topic><topic>T-Lymphocytes - virology</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zábranský, Aleš</creatorcontrib><creatorcontrib>Hadravová, Romana</creatorcontrib><creatorcontrib>Štokrová, Jitka</creatorcontrib><creatorcontrib>Sakalian, Michael</creatorcontrib><creatorcontrib>Pichová, Iva</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zábranský, Aleš</au><au>Hadravová, Romana</au><au>Štokrová, Jitka</au><au>Sakalian, Michael</au><au>Pichová, Iva</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Premature processing of mouse mammary tumor virus Gag polyprotein impairs intracellular capsid assembly</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>2009-02-05</date><risdate>2009</risdate><volume>384</volume><issue>1</issue><spage>33</spage><epage>37</epage><pages>33-37</pages><issn>0042-6822</issn><eissn>1096-0341</eissn><abstract>Abstract Mouse mammary tumor virus (MMTV) is the prototypical member of the Betaretrovirus genus, but the processes of its morphogenesis are poorly characterized. In this report, we describe an unusual intracellular processing of MMTV Gag polyprotein in human 293T cells transiently expressing MMTV from heterologous promoter. The same specific cleavage products of the viral protease were seen for the wild type as well as for nonmyristylated mutant of MMTV Gag polyprotein completely defective in the particle release. Inactivation of the viral protease resulted in more stable Gag polyprotein and in accumulation of intracytoplasmic particles for nonmyristylated Gag. The intracellular processing of nonmyristylated MMTV Gag indicates that protease activation in betaretrovirus can occur independently of budding.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>19046754</pmid><doi>10.1016/j.virol.2008.10.038</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete; EZB Electronic Journals Library |
| subjects | Amino Acid Substitution Animals Assembly Betaretrovirus Dexamethasone - pharmacology Female Gag Gene Products, gag - genetics Gene Products, gag - metabolism Humans Infectious Disease Kinetics Mammary Glands, Animal - virology Mammary Neoplasms, Animal - virology Mammary Neoplasms, Experimental - virology Mammary Tumor Virus, Mouse - genetics Mammary Tumor Virus, Mouse - metabolism Mice MMTV Mouse mammary tumor virus Peptide Hydrolases - genetics Peptide Hydrolases - metabolism Promoter Regions, Genetic Proviruses - genetics Restriction Mapping T-Lymphocytes - drug effects T-Lymphocytes - virology Transfection |
| title | Premature processing of mouse mammary tumor virus Gag polyprotein impairs intracellular capsid assembly |
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