Glucose Uptake Activity in Murine Red Blood Cells Infected with Babesia microti and Babesia rodhaini
The glucose uptake activity in Babesia rodhaini and B. microti - infected red blood cell (IRBC) was investigated in mice using 2-deoxy-D-glucose (2DOG) and L-glucose (L-Glc), a non-metabolizable analogue of D-glucose and non-incorporative glucose to non-infected RBC (NRBC), respectively. The uptake...
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| Veröffentlicht in: | Journal of Veterinary Medical Science 2004, Vol.66(8), pp.945-949 |
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| creator | OHMORI, Takashi ADACHI, Keishi FUKUDA, Yoshimitsu TAMAHARA, Satoshi MATSUKI, Naoaki ONO, Kenichiro |
| description | The glucose uptake activity in Babesia rodhaini and B. microti - infected red blood cell (IRBC) was investigated in mice using 2-deoxy-D-glucose (2DOG) and L-glucose (L-Glc), a non-metabolizable analogue of D-glucose and non-incorporative glucose to non-infected RBC (NRBC), respectively. The uptake activities of both DOG and L-Glc were higher in IRBCs than those in NRBC. The concentration dependent uptake of 2DOG and L-Glc in both IRBC revealed a linear curve, indicating non-transporter mediated uptake. In addition, B. microti IRBC showed higher 2DOG uptake than B. rodhaini IRBC, whereas no difference was observed in L-Glc uptake. These results indicated that some new glucose uptake system, at least two systems, developed in both IRBC. The new systems were sodium independent, non-competitive to L-Glc, and sensitive to temperature. One of two systems had no kinetical difference between B. rodhaini and B. microti IRBC, however another one might have higher uptake activity in B. microti IRBC compared to that in B. rodhaini IRBC. |
| doi_str_mv | 10.1292/jvms.66.945 |
| format | Article |
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The uptake activities of both DOG and L-Glc were higher in IRBCs than those in NRBC. The concentration dependent uptake of 2DOG and L-Glc in both IRBC revealed a linear curve, indicating non-transporter mediated uptake. In addition, B. microti IRBC showed higher 2DOG uptake than B. rodhaini IRBC, whereas no difference was observed in L-Glc uptake. These results indicated that some new glucose uptake system, at least two systems, developed in both IRBC. The new systems were sodium independent, non-competitive to L-Glc, and sensitive to temperature. One of two systems had no kinetical difference between B. rodhaini and B. microti IRBC, however another one might have higher uptake activity in B. microti IRBC compared to that in B. rodhaini IRBC.</description><identifier>ISSN: 0916-7250</identifier><identifier>EISSN: 1347-7439</identifier><identifier>DOI: 10.1292/jvms.66.945</identifier><identifier>PMID: 15353845</identifier><language>eng</language><publisher>Japan: JAPANESE SOCIETY OF VETERINARY SCIENCE</publisher><subject>Animals ; Babesia microti ; Babesia rodhaini ; Erythrocytes - metabolism ; Erythrocytes - parasitology ; Glucose - pharmacokinetics ; glucose uptake ; Isomerism ; Kinetics ; Mice - metabolism ; Mice - parasitology ; murine ; RBC ; Sodium - metabolism ; Temperature</subject><ispartof>Journal of Veterinary Medical Science, 2004, Vol.66(8), pp.945-949</ispartof><rights>2004 by the Japanese Society of Veterinary Science</rights><rights>Copyright Japan Science and Technology Agency 2004</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c528t-17155f472d718f57b6bf23cb6ef07c243c52fceb660c17f2d1d978af98b78d6a3</citedby><cites>FETCH-LOGICAL-c528t-17155f472d718f57b6bf23cb6ef07c243c52fceb660c17f2d1d978af98b78d6a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15353845$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>OHMORI, Takashi</creatorcontrib><creatorcontrib>ADACHI, Keishi</creatorcontrib><creatorcontrib>FUKUDA, Yoshimitsu</creatorcontrib><creatorcontrib>TAMAHARA, Satoshi</creatorcontrib><creatorcontrib>MATSUKI, Naoaki</creatorcontrib><creatorcontrib>ONO, Kenichiro</creatorcontrib><title>Glucose Uptake Activity in Murine Red Blood Cells Infected with Babesia microti and Babesia rodhaini</title><title>Journal of Veterinary Medical Science</title><addtitle>J. Vet. Med. Sci.</addtitle><description>The glucose uptake activity in Babesia rodhaini and B. microti - infected red blood cell (IRBC) was investigated in mice using 2-deoxy-D-glucose (2DOG) and L-glucose (L-Glc), a non-metabolizable analogue of D-glucose and non-incorporative glucose to non-infected RBC (NRBC), respectively. The uptake activities of both DOG and L-Glc were higher in IRBCs than those in NRBC. The concentration dependent uptake of 2DOG and L-Glc in both IRBC revealed a linear curve, indicating non-transporter mediated uptake. In addition, B. microti IRBC showed higher 2DOG uptake than B. rodhaini IRBC, whereas no difference was observed in L-Glc uptake. These results indicated that some new glucose uptake system, at least two systems, developed in both IRBC. The new systems were sodium independent, non-competitive to L-Glc, and sensitive to temperature. One of two systems had no kinetical difference between B. rodhaini and B. microti IRBC, however another one might have higher uptake activity in B. microti IRBC compared to that in B. rodhaini IRBC.</description><subject>Animals</subject><subject>Babesia microti</subject><subject>Babesia rodhaini</subject><subject>Erythrocytes - metabolism</subject><subject>Erythrocytes - parasitology</subject><subject>Glucose - pharmacokinetics</subject><subject>glucose uptake</subject><subject>Isomerism</subject><subject>Kinetics</subject><subject>Mice - metabolism</subject><subject>Mice - parasitology</subject><subject>murine</subject><subject>RBC</subject><subject>Sodium - metabolism</subject><subject>Temperature</subject><issn>0916-7250</issn><issn>1347-7439</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctrVDEYxYNY7LS6ci8BoZtyxzxuXru2g9ZCiyB2HXLzcDK9j2mSW-l_b8oMU3DjJoGTHyffdw4AHzFaYqLIl83TkJecL1XL3oAFpq1oREvVW7BACvNGEIaOwUnOG4QIbrl6B44xo4zKli2Au-5nO2UP77fFPHh4aUt8iuUZxhHezSmOHv70Dl710-Tgyvd9hjdj8LZU8U8sa3hlOp-jgUO0aSoRmtEdtDS5tYljfA-Ogumz_7C_T8H9t6-_Vt-b2x_XN6vL28YyIkuDBWYstII4gWVgouNdINR23AckLGlpxYL1HefIYhGIw04JaYKSnZCOG3oKzna-2zQ9zj4XPcRs69Bm9NOcNeeSMaTYf0EsRI2Oogp-_gfcTHMa6xK6Rim5koKKSp3vqBpBzskHvU1xMOlZY6RfOtIvHdXvde2o0p_2nnM3ePfK7kupwMUO2ORifvsDYFKJtvcHM7k7qufhya5N0n6kfwEK96QD</recordid><startdate>20040801</startdate><enddate>20040801</enddate><creator>OHMORI, Takashi</creator><creator>ADACHI, Keishi</creator><creator>FUKUDA, Yoshimitsu</creator><creator>TAMAHARA, Satoshi</creator><creator>MATSUKI, Naoaki</creator><creator>ONO, Kenichiro</creator><general>JAPANESE SOCIETY OF VETERINARY SCIENCE</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20040801</creationdate><title>Glucose Uptake Activity in Murine Red Blood Cells Infected with Babesia microti and Babesia rodhaini</title><author>OHMORI, Takashi ; ADACHI, Keishi ; FUKUDA, Yoshimitsu ; TAMAHARA, Satoshi ; MATSUKI, Naoaki ; ONO, Kenichiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c528t-17155f472d718f57b6bf23cb6ef07c243c52fceb660c17f2d1d978af98b78d6a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Babesia microti</topic><topic>Babesia rodhaini</topic><topic>Erythrocytes - metabolism</topic><topic>Erythrocytes - parasitology</topic><topic>Glucose - pharmacokinetics</topic><topic>glucose uptake</topic><topic>Isomerism</topic><topic>Kinetics</topic><topic>Mice - metabolism</topic><topic>Mice - parasitology</topic><topic>murine</topic><topic>RBC</topic><topic>Sodium - metabolism</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>OHMORI, Takashi</creatorcontrib><creatorcontrib>ADACHI, Keishi</creatorcontrib><creatorcontrib>FUKUDA, Yoshimitsu</creatorcontrib><creatorcontrib>TAMAHARA, Satoshi</creatorcontrib><creatorcontrib>MATSUKI, Naoaki</creatorcontrib><creatorcontrib>ONO, Kenichiro</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Veterinary Medical Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>OHMORI, Takashi</au><au>ADACHI, Keishi</au><au>FUKUDA, Yoshimitsu</au><au>TAMAHARA, Satoshi</au><au>MATSUKI, Naoaki</au><au>ONO, Kenichiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Glucose Uptake Activity in Murine Red Blood Cells Infected with Babesia microti and Babesia rodhaini</atitle><jtitle>Journal of Veterinary Medical Science</jtitle><addtitle>J. Vet. Med. Sci.</addtitle><date>2004-08-01</date><risdate>2004</risdate><volume>66</volume><issue>8</issue><spage>945</spage><epage>949</epage><pages>945-949</pages><issn>0916-7250</issn><eissn>1347-7439</eissn><abstract>The glucose uptake activity in Babesia rodhaini and B. microti - infected red blood cell (IRBC) was investigated in mice using 2-deoxy-D-glucose (2DOG) and L-glucose (L-Glc), a non-metabolizable analogue of D-glucose and non-incorporative glucose to non-infected RBC (NRBC), respectively. The uptake activities of both DOG and L-Glc were higher in IRBCs than those in NRBC. The concentration dependent uptake of 2DOG and L-Glc in both IRBC revealed a linear curve, indicating non-transporter mediated uptake. In addition, B. microti IRBC showed higher 2DOG uptake than B. rodhaini IRBC, whereas no difference was observed in L-Glc uptake. These results indicated that some new glucose uptake system, at least two systems, developed in both IRBC. The new systems were sodium independent, non-competitive to L-Glc, and sensitive to temperature. One of two systems had no kinetical difference between B. rodhaini and B. microti IRBC, however another one might have higher uptake activity in B. microti IRBC compared to that in B. rodhaini IRBC.</abstract><cop>Japan</cop><pub>JAPANESE SOCIETY OF VETERINARY SCIENCE</pub><pmid>15353845</pmid><doi>10.1292/jvms.66.945</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Babesia microti Babesia rodhaini Erythrocytes - metabolism Erythrocytes - parasitology Glucose - pharmacokinetics glucose uptake Isomerism Kinetics Mice - metabolism Mice - parasitology murine RBC Sodium - metabolism Temperature |
| title | Glucose Uptake Activity in Murine Red Blood Cells Infected with Babesia microti and Babesia rodhaini |
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