Spectroscopic characterization of fluorescein- and tetramethylrhodamine-labeled oligonucleotides and their complexes with a DNA template

We measured absorption and emission spectra, fluorescence quantum yield, anisotropy, fluorescence resonance energy transfer (FRET), and melting temperature to characterize fluorescein- and tetramethylrhodamine (TMR)-labeled oligonucleotides in solution and when hybridized to a common DNA template. U...

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Veröffentlicht in:	Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Molecular and biomolecular spectroscopy, 2004-10, Vol.60 (12), p.2741-2750
Hauptverfasser:	Wang, L., Gaigalas, A.K., Blasic, J., Holden, M.J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence DNA - chemistry DNA complexes Fluorescein Fluorescein - chemistry Fluorescence quenching Fluorescence resonance energy transfer Fluorescent Dyes Melting temperature Models, Chemical Molecular Structure Nucleic Acid Hybridization Oligodeoxyribonucleotides - chemistry Rhodamines - chemistry Spectrometry, Fluorescence Spectrophotometry Tetramethylrhodamine
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