Molecular cytogenetic characterization of the mantle cell lymphoma cell line GRANTA-519
Combining fluorescence R-banding, fluorescence in situ hybridization and spectral karyotyping allowed us to precisely define chromosomal breakpoints, gains, losses and a newly detected amplification in the human mantle cell lymphoma (MCL) cell line GRANTA-519. GRANTA-519 is characterized by the t(11...
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| Veröffentlicht in: | Cancer genetics and cytogenetics 2004-09, Vol.153 (2), p.144-150 |
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| creator | Rudolph, C. Steinemann, D. von Neuhoff, N. Gadzicki, D. Ripperger, T. Drexler, H.G. Mrasek, K. Liehr, T. Claussen, U. Emura, M. Schrock, E. Schlegelberger, B. |
| description | Combining fluorescence R-banding, fluorescence in situ hybridization and spectral karyotyping allowed us to precisely define chromosomal breakpoints, gains, losses and a newly detected amplification in the human mantle cell lymphoma (MCL) cell line GRANTA-519. GRANTA-519 is characterized by the t(11;14)(q13;q32) resulting in overexpression of cyclin D1, a key player in cell cycle control. Hitherto unresolved complex rearrangements involve 1p, 1q, 3cen, 9p, 11q, 12p, 12q, 16p, 17p, and 18cen. Moreover, a 4- to 6-fold gain of sequences on 18q leads to a low-level amplification of the
BCL2 gene and to an overexpression of the BCL2 protein. These results provide the basis for the identification of not only candidate oncogenes responsible for MCL in gained regions, but also for the identification of putative tumor suppressor genes in commonly deleted regions like 1p22, which would eventually enable functional studies of these genes. |
| doi_str_mv | 10.1016/j.cancergencyto.2004.01.006 |
| format | Article |
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BCL2 gene and to an overexpression of the BCL2 protein. These results provide the basis for the identification of not only candidate oncogenes responsible for MCL in gained regions, but also for the identification of putative tumor suppressor genes in commonly deleted regions like 1p22, which would eventually enable functional studies of these genes.</description><identifier>ISSN: 0165-4608</identifier><identifier>EISSN: 1873-4456</identifier><identifier>DOI: 10.1016/j.cancergencyto.2004.01.006</identifier><identifier>PMID: 15350304</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Cell Culture Techniques - methods ; Cell Line, Tumor ; Chromosome Banding ; Chromosome Mapping ; Cytogenetics ; Humans ; In Situ Hybridization, Fluorescence ; Karyotyping ; Lymphoma, Mantle-Cell - genetics ; Lymphoma, Mantle-Cell - pathology ; Metaphase ; Translocation, Genetic - genetics</subject><ispartof>Cancer genetics and cytogenetics, 2004-09, Vol.153 (2), p.144-150</ispartof><rights>2004 Elsevier Inc.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c410t-f1fdb59d8e37ad2a1b88515066156989644a40f6c3306d761382d897522e24c33</citedby><cites>FETCH-LOGICAL-c410t-f1fdb59d8e37ad2a1b88515066156989644a40f6c3306d761382d897522e24c33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0165460804000251$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15350304$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rudolph, C.</creatorcontrib><creatorcontrib>Steinemann, D.</creatorcontrib><creatorcontrib>von Neuhoff, N.</creatorcontrib><creatorcontrib>Gadzicki, D.</creatorcontrib><creatorcontrib>Ripperger, T.</creatorcontrib><creatorcontrib>Drexler, H.G.</creatorcontrib><creatorcontrib>Mrasek, K.</creatorcontrib><creatorcontrib>Liehr, T.</creatorcontrib><creatorcontrib>Claussen, U.</creatorcontrib><creatorcontrib>Emura, M.</creatorcontrib><creatorcontrib>Schrock, E.</creatorcontrib><creatorcontrib>Schlegelberger, B.</creatorcontrib><title>Molecular cytogenetic characterization of the mantle cell lymphoma cell line GRANTA-519</title><title>Cancer genetics and cytogenetics</title><addtitle>Cancer Genet Cytogenet</addtitle><description>Combining fluorescence R-banding, fluorescence in situ hybridization and spectral karyotyping allowed us to precisely define chromosomal breakpoints, gains, losses and a newly detected amplification in the human mantle cell lymphoma (MCL) cell line GRANTA-519. GRANTA-519 is characterized by the t(11;14)(q13;q32) resulting in overexpression of cyclin D1, a key player in cell cycle control. Hitherto unresolved complex rearrangements involve 1p, 1q, 3cen, 9p, 11q, 12p, 12q, 16p, 17p, and 18cen. Moreover, a 4- to 6-fold gain of sequences on 18q leads to a low-level amplification of the
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These results provide the basis for the identification of not only candidate oncogenes responsible for MCL in gained regions, but also for the identification of putative tumor suppressor genes in commonly deleted regions like 1p22, which would eventually enable functional studies of these genes.</description><subject>Cell Culture Techniques - methods</subject><subject>Cell Line, Tumor</subject><subject>Chromosome Banding</subject><subject>Chromosome Mapping</subject><subject>Cytogenetics</subject><subject>Humans</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Karyotyping</subject><subject>Lymphoma, Mantle-Cell - genetics</subject><subject>Lymphoma, Mantle-Cell - pathology</subject><subject>Metaphase</subject><subject>Translocation, Genetic - genetics</subject><issn>0165-4608</issn><issn>1873-4456</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUFr3DAQhUVpSbZp_kIxFHqzO2NLskxPS0iTQtJCSOhRaOVxV4ttbSVtYfvro2UXSk_NaZjhmzfDe4x9QKgQUH7aVNbMlsJPmu0--aoG4BVgBSBfsQWqtik5F_I1W2RalFyCOmdvY9wAQFt38oydo2gENMAX7Me9H8nuRhOKg1jWpORsYdcmGJsouD8mOT8XfijSmorJzGmkwtI4FuN-2q79ZE6dm6m4eVh-e1yWArt37M1gxkiXp3rBnr5cP17dlnffb75eLe9KyxFSOeDQr0TXK2pa09cGV0oJFCAlCtmpTnJuOAzSNg3IvpXYqLpXXSvqmmqepxfs41F3G_yvHcWkJxcPD5mZ_C5qKZWAFv4PYtdy4AIz-PkI2uBjDDTobXCTCXuNoA8B6I3-JwB9CEAD6hxA3n5_OrNbTdT_3T05noHrI0DZld-Ogo7WZSHqXSCbdO_diw49AxsXnSA</recordid><startdate>20040901</startdate><enddate>20040901</enddate><creator>Rudolph, C.</creator><creator>Steinemann, D.</creator><creator>von Neuhoff, N.</creator><creator>Gadzicki, D.</creator><creator>Ripperger, T.</creator><creator>Drexler, H.G.</creator><creator>Mrasek, K.</creator><creator>Liehr, T.</creator><creator>Claussen, U.</creator><creator>Emura, M.</creator><creator>Schrock, E.</creator><creator>Schlegelberger, B.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20040901</creationdate><title>Molecular cytogenetic characterization of the mantle cell lymphoma cell line GRANTA-519</title><author>Rudolph, C. ; 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| subjects | Cell Culture Techniques - methods Cell Line, Tumor Chromosome Banding Chromosome Mapping Cytogenetics Humans In Situ Hybridization, Fluorescence Karyotyping Lymphoma, Mantle-Cell - genetics Lymphoma, Mantle-Cell - pathology Metaphase Translocation, Genetic - genetics |
| title | Molecular cytogenetic characterization of the mantle cell lymphoma cell line GRANTA-519 |
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