Effect of antisense oligodeoxynucleotide of small subunit component of human ribonucleotide reductase on human choriocarcinoma cell line in vitro
To study effect of the antisense oligodeoxynucleotide (ASODN) of small subunit of human ribonucleotide reductase (RRM2) mRNA on cell line of human choriocarcinoma in vitro. Two 20-mer gapmer ASODNs with a full phosphorothioate backbone were artificially synthesized, which were complementary to nucle...
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| Veröffentlicht in: | Chung-hua fu chʿan kʿo tsa chih 2004-07, Vol.39 (7), p.465-468 |
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| container_title | Chung-hua fu chʿan kʿo tsa chih |
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| creator | Cui, Jin-Quan Shi, Yi-Fu Zhou, Huai-Jun |
| description | To study effect of the antisense oligodeoxynucleotide (ASODN) of small subunit of human ribonucleotide reductase (RRM2) mRNA on cell line of human choriocarcinoma in vitro.
Two 20-mer gapmer ASODNs with a full phosphorothioate backbone were artificially synthesized, which were complementary to nucleotides 626-645 (a coding region) and 1572-1591 (a 3'untranslated region) of RRM2, respectively. ASODNs were transfected into JAR cells through oligofectamine. The survival rate was assessed by methyl thiazolyl tetrazolium (MMT) assay, and RRM2 expression was detected by immunoblot and reverse transcriptase polymerase chain reaction (RT-PCR) methods.
Antisense oligodeoxynucleotide one (ASODN1) targeting the coding region significantly inhibited growth of JAR cells in a dose- and time-dependent manner and downregulated RRM2 expression in a time-dependent manner. ASODN1 at 100 nmol/L could inhibit significantly cell growth (P = 0.000), and the effects of ASODN1 on JAR cell proliferation were enhanced with increase of |
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Two 20-mer gapmer ASODNs with a full phosphorothioate backbone were artificially synthesized, which were complementary to nucleotides 626-645 (a coding region) and 1572-1591 (a 3'untranslated region) of RRM2, respectively. ASODNs were transfected into JAR cells through oligofectamine. The survival rate was assessed by methyl thiazolyl tetrazolium (MMT) assay, and RRM2 expression was detected by immunoblot and reverse transcriptase polymerase chain reaction (RT-PCR) methods.
Antisense oligodeoxynucleotide one (ASODN1) targeting the coding region significantly inhibited growth of JAR cells in a dose- and time-dependent manner and downregulated RRM2 expression in a time-dependent manner. ASODN1 at 100 nmol/L could inhibit significantly cell growth (P = 0.000), and the effects of ASODN1 on JAR cell proliferation were enhanced with increase of</description><identifier>ISSN: 0529-567X</identifier><identifier>PMID: 15347470</identifier><language>chi</language><publisher>China</publisher><subject>Adult ; Antineoplastic Agents - pharmacology ; Cell Division - drug effects ; Choriocarcinoma - pathology ; Female ; Humans ; Oligodeoxyribonucleotides, Antisense - pharmacology ; Pregnancy ; Ribonucleotide Reductases - pharmacology ; RNA, Messenger - pharmacology ; Tumor Cells, Cultured ; Uterine Neoplasms - pathology</subject><ispartof>Chung-hua fu chʿan kʿo tsa chih, 2004-07, Vol.39 (7), p.465-468</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15347470$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cui, Jin-Quan</creatorcontrib><creatorcontrib>Shi, Yi-Fu</creatorcontrib><creatorcontrib>Zhou, Huai-Jun</creatorcontrib><title>Effect of antisense oligodeoxynucleotide of small subunit component of human ribonucleotide reductase on human choriocarcinoma cell line in vitro</title><title>Chung-hua fu chʿan kʿo tsa chih</title><addtitle>Zhonghua Fu Chan Ke Za Zhi</addtitle><description>To study effect of the antisense oligodeoxynucleotide (ASODN) of small subunit of human ribonucleotide reductase (RRM2) mRNA on cell line of human choriocarcinoma in vitro.
Two 20-mer gapmer ASODNs with a full phosphorothioate backbone were artificially synthesized, which were complementary to nucleotides 626-645 (a coding region) and 1572-1591 (a 3'untranslated region) of RRM2, respectively. ASODNs were transfected into JAR cells through oligofectamine. The survival rate was assessed by methyl thiazolyl tetrazolium (MMT) assay, and RRM2 expression was detected by immunoblot and reverse transcriptase polymerase chain reaction (RT-PCR) methods.
Antisense oligodeoxynucleotide one (ASODN1) targeting the coding region significantly inhibited growth of JAR cells in a dose- and time-dependent manner and downregulated RRM2 expression in a time-dependent manner. ASODN1 at 100 nmol/L could inhibit significantly cell growth (P = 0.000), and the effects of ASODN1 on JAR cell proliferation were enhanced with increase of</description><subject>Adult</subject><subject>Antineoplastic Agents - pharmacology</subject><subject>Cell Division - drug effects</subject><subject>Choriocarcinoma - pathology</subject><subject>Female</subject><subject>Humans</subject><subject>Oligodeoxyribonucleotides, Antisense - pharmacology</subject><subject>Pregnancy</subject><subject>Ribonucleotide Reductases - pharmacology</subject><subject>RNA, Messenger - pharmacology</subject><subject>Tumor Cells, Cultured</subject><subject>Uterine Neoplasms - pathology</subject><issn>0529-567X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkM1KxDAUhbNQnGGcV5Cs3BWatmnapQzjDwzMRsFduUlunUCb1PyI8xi-sR0dwdWFw3c-DveCLHNetBmvxeuCrEMwMuesrJuCtVdkwXhZiUrkS_K17XtUkbqego0moA1I3WDenEb3ebRJDeii0XgiwgjDQEOSyZpIlRsnZ9H-lA9pBEu9ke5fxaNOKsLJaM-EOjhvnAKvjHUjUIWzcTAWqbH0w0TvrsllD0PA9fmuyMv99nnzmO32D0-bu102saKKGWqZt1yUWmulRcEV1Mg0b-dA5AAoWC77RkGhdFOWIFHIRvV8BivVMtDlitz-eifv3hOG2I0mnOaARZdCV9dN1bQFn8GbM5jkiLqbvBnBH7u_J5bfvk1z2Q</recordid><startdate>200407</startdate><enddate>200407</enddate><creator>Cui, Jin-Quan</creator><creator>Shi, Yi-Fu</creator><creator>Zhou, Huai-Jun</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>200407</creationdate><title>Effect of antisense oligodeoxynucleotide of small subunit component of human ribonucleotide reductase on human choriocarcinoma cell line in vitro</title><author>Cui, Jin-Quan ; Shi, Yi-Fu ; Zhou, Huai-Jun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p124t-edb09573dddcd725ca6e1d593dd70aae710bf8ca2cd833abe7b8cf525c4c91ad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2004</creationdate><topic>Adult</topic><topic>Antineoplastic Agents - pharmacology</topic><topic>Cell Division - drug effects</topic><topic>Choriocarcinoma - pathology</topic><topic>Female</topic><topic>Humans</topic><topic>Oligodeoxyribonucleotides, Antisense - pharmacology</topic><topic>Pregnancy</topic><topic>Ribonucleotide Reductases - pharmacology</topic><topic>RNA, Messenger - pharmacology</topic><topic>Tumor Cells, Cultured</topic><topic>Uterine Neoplasms - pathology</topic><toplevel>online_resources</toplevel><creatorcontrib>Cui, Jin-Quan</creatorcontrib><creatorcontrib>Shi, Yi-Fu</creatorcontrib><creatorcontrib>Zhou, Huai-Jun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Chung-hua fu chʿan kʿo tsa chih</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cui, Jin-Quan</au><au>Shi, Yi-Fu</au><au>Zhou, Huai-Jun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of antisense oligodeoxynucleotide of small subunit component of human ribonucleotide reductase on human choriocarcinoma cell line in vitro</atitle><jtitle>Chung-hua fu chʿan kʿo tsa chih</jtitle><addtitle>Zhonghua Fu Chan Ke Za Zhi</addtitle><date>2004-07</date><risdate>2004</risdate><volume>39</volume><issue>7</issue><spage>465</spage><epage>468</epage><pages>465-468</pages><issn>0529-567X</issn><abstract>To study effect of the antisense oligodeoxynucleotide (ASODN) of small subunit of human ribonucleotide reductase (RRM2) mRNA on cell line of human choriocarcinoma in vitro.
Two 20-mer gapmer ASODNs with a full phosphorothioate backbone were artificially synthesized, which were complementary to nucleotides 626-645 (a coding region) and 1572-1591 (a 3'untranslated region) of RRM2, respectively. ASODNs were transfected into JAR cells through oligofectamine. The survival rate was assessed by methyl thiazolyl tetrazolium (MMT) assay, and RRM2 expression was detected by immunoblot and reverse transcriptase polymerase chain reaction (RT-PCR) methods.
Antisense oligodeoxynucleotide one (ASODN1) targeting the coding region significantly inhibited growth of JAR cells in a dose- and time-dependent manner and downregulated RRM2 expression in a time-dependent manner. ASODN1 at 100 nmol/L could inhibit significantly cell growth (P = 0.000), and the effects of ASODN1 on JAR cell proliferation were enhanced with increase of</abstract><cop>China</cop><pmid>15347470</pmid><tpages>4</tpages></addata></record> |
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| ispartof | Chung-hua fu chʿan kʿo tsa chih, 2004-07, Vol.39 (7), p.465-468 |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Adult Antineoplastic Agents - pharmacology Cell Division - drug effects Choriocarcinoma - pathology Female Humans Oligodeoxyribonucleotides, Antisense - pharmacology Pregnancy Ribonucleotide Reductases - pharmacology RNA, Messenger - pharmacology Tumor Cells, Cultured Uterine Neoplasms - pathology |
| title | Effect of antisense oligodeoxynucleotide of small subunit component of human ribonucleotide reductase on human choriocarcinoma cell line in vitro |
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