Molecular characterization and real-time PCR transcriptional analysis of Dictyocaulus viviparus major sperm proteins
Major sperm proteins (MSPs) represent a protein family occurring in nematodes only. Identification of the 3' and 5' untranslated region (UTR) completed the so far partial msp complementary DNA sequences of the bovine lungworm Dictyocaulus viviparus. The full-length transcript contains sequ...
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| Veröffentlicht in: | Parasitology research (1987) 2009-02, Vol.104 (3), p.543-551 |
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| creator | Strube, Christina Buschbaum, Sandra Schnieder, Thomas |
| description | Major sperm proteins (MSPs) represent a protein family occurring in nematodes only. Identification of the 3' and 5' untranslated region (UTR) completed the so far partial msp complementary DNA sequences of the bovine lungworm Dictyocaulus viviparus. The full-length transcript contains sequence tracts consistent with the Kozak and polyadenylation consensus sequence. On genomic level, three full-length sequences differing in three nucleotides were determined containing a 65-bp phase zero intron. Conceptual translation inferred two MSP isoforms due to one substitution within the 126-amino acid polypeptide. Bioinformatic analysis predicted that bovine lungworm MSP folds into an immunoglobulin-like seven-stranded beta sandwich as known for Caenorhabditis elegans and Ascaris suum. Furthermore, bovine lungworm MSP is confidentially predicted to be N-terminal-acetylated and secreted via a non-classical pathway. Quantitative real-time polymerase chain reaction analysis using ten developmental lungworm stages showed that msp is transcribed mainly in adult male parasites and in some degree in hypobiotic L5. However, marginal msp transcription was detectable in all of the investigated developmental lungworm stages. |
| doi_str_mv | 10.1007/s00436-008-1228-5 |
| format | Article |
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Identification of the 3' and 5' untranslated region (UTR) completed the so far partial msp complementary DNA sequences of the bovine lungworm Dictyocaulus viviparus. The full-length transcript contains sequence tracts consistent with the Kozak and polyadenylation consensus sequence. On genomic level, three full-length sequences differing in three nucleotides were determined containing a 65-bp phase zero intron. Conceptual translation inferred two MSP isoforms due to one substitution within the 126-amino acid polypeptide. Bioinformatic analysis predicted that bovine lungworm MSP folds into an immunoglobulin-like seven-stranded beta sandwich as known for Caenorhabditis elegans and Ascaris suum. Furthermore, bovine lungworm MSP is confidentially predicted to be N-terminal-acetylated and secreted via a non-classical pathway. Quantitative real-time polymerase chain reaction analysis using ten developmental lungworm stages showed that msp is transcribed mainly in adult male parasites and in some degree in hypobiotic L5. However, marginal msp transcription was detectable in all of the investigated developmental lungworm stages.</description><identifier>ISSN: 0932-0113</identifier><identifier>EISSN: 1432-1955</identifier><identifier>DOI: 10.1007/s00436-008-1228-5</identifier><identifier>PMID: 18853187</identifier><identifier>CODEN: PARREZ</identifier><language>eng</language><publisher>Berlin/Heidelberg: Berlin/Heidelberg : Springer-Verlag</publisher><subject>3' Untranslated Regions ; 5' Untranslated Regions ; Amino Acid Sequence ; Amino acid substitution ; Amino Acid Substitution - genetics ; Animals ; Biological and medical sciences ; Biomedical and Life Sciences ; Biomedicine ; Cattle ; Classical pathway ; Complementary DNA ; Computational Biology ; Conserved sequence ; Dictyocaulus - chemistry ; Dictyocaulus - genetics ; Dictyocaulus viviparus ; DNA, Complementary ; DNA, Helminth - chemistry ; DNA, Helminth - genetics ; Fundamental and applied biological sciences. Psychology ; Gene Expression Profiling ; General aspects ; General aspects and techniques. Study of several systematic groups. Models ; Genomics ; Helminth Proteins - biosynthesis ; Helminth Proteins - genetics ; Immunology ; Introns ; Invertebrates ; Isoforms ; Medical Microbiology ; Microbiology ; Models, Molecular ; Molecular Sequence Data ; Nucleotide sequence ; Original Paper ; Polyadenylation ; Polymerase chain reaction ; Polymerase Chain Reaction - methods ; Protein Folding ; Protein Isoforms - genetics ; Protein Structure, Tertiary ; Sequence Alignment ; Sequence Analysis, DNA ; Sperm ; Transcription</subject><ispartof>Parasitology research (1987), 2009-02, Vol.104 (3), p.543-551</ispartof><rights>Springer-Verlag 2008</rights><rights>2009 INIST-CNRS</rights><rights>Springer-Verlag 2008.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c467t-44ae722871d8516db3f56ce47cecb086023844e3f9635213858293bfe620d8013</citedby><cites>FETCH-LOGICAL-c467t-44ae722871d8516db3f56ce47cecb086023844e3f9635213858293bfe620d8013</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00436-008-1228-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00436-008-1228-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21092968$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18853187$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Strube, Christina</creatorcontrib><creatorcontrib>Buschbaum, Sandra</creatorcontrib><creatorcontrib>Schnieder, Thomas</creatorcontrib><title>Molecular characterization and real-time PCR transcriptional analysis of Dictyocaulus viviparus major sperm proteins</title><title>Parasitology research (1987)</title><addtitle>Parasitol Res</addtitle><addtitle>Parasitol Res</addtitle><description>Major sperm proteins (MSPs) represent a protein family occurring in nematodes only. Identification of the 3' and 5' untranslated region (UTR) completed the so far partial msp complementary DNA sequences of the bovine lungworm Dictyocaulus viviparus. The full-length transcript contains sequence tracts consistent with the Kozak and polyadenylation consensus sequence. On genomic level, three full-length sequences differing in three nucleotides were determined containing a 65-bp phase zero intron. Conceptual translation inferred two MSP isoforms due to one substitution within the 126-amino acid polypeptide. Bioinformatic analysis predicted that bovine lungworm MSP folds into an immunoglobulin-like seven-stranded beta sandwich as known for Caenorhabditis elegans and Ascaris suum. Furthermore, bovine lungworm MSP is confidentially predicted to be N-terminal-acetylated and secreted via a non-classical pathway. Quantitative real-time polymerase chain reaction analysis using ten developmental lungworm stages showed that msp is transcribed mainly in adult male parasites and in some degree in hypobiotic L5. However, marginal msp transcription was detectable in all of the investigated developmental lungworm stages.</description><subject>3' Untranslated Regions</subject><subject>5' Untranslated Regions</subject><subject>Amino Acid Sequence</subject><subject>Amino acid substitution</subject><subject>Amino Acid Substitution - genetics</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cattle</subject><subject>Classical pathway</subject><subject>Complementary DNA</subject><subject>Computational Biology</subject><subject>Conserved sequence</subject><subject>Dictyocaulus - chemistry</subject><subject>Dictyocaulus - genetics</subject><subject>Dictyocaulus viviparus</subject><subject>DNA, Complementary</subject><subject>DNA, Helminth - chemistry</subject><subject>DNA, Helminth - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Profiling</subject><subject>General aspects</subject><subject>General aspects and techniques. Study of several systematic groups. Models</subject><subject>Genomics</subject><subject>Helminth Proteins - biosynthesis</subject><subject>Helminth Proteins - genetics</subject><subject>Immunology</subject><subject>Introns</subject><subject>Invertebrates</subject><subject>Isoforms</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>Models, Molecular</subject><subject>Molecular Sequence Data</subject><subject>Nucleotide sequence</subject><subject>Original Paper</subject><subject>Polyadenylation</subject><subject>Polymerase chain reaction</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Protein Folding</subject><subject>Protein Isoforms - genetics</subject><subject>Protein Structure, Tertiary</subject><subject>Sequence Alignment</subject><subject>Sequence Analysis, DNA</subject><subject>Sperm</subject><subject>Transcription</subject><issn>0932-0113</issn><issn>1432-1955</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1v1DAQhi0EotvCD-ACllC5BTz-inNEy6dUBAJ6tmYdp3iVxFs7qbT8ehxlRSUOnGakeebrfQl5Buw1MFa_yYxJoSvGTAWcm0o9IBuQglfQKPWQbFhTcgYgzsh5znvGoNZSPiZnYIwSYOoNmb7E3ru5x0TdL0zoJp_Cb5xCHCmOLU0e-2oKg6fftt_plHDMLoXDUse-ENgfc8g0dvRdcNMxOpz7OdO7cBcOmEo24D4mmg8-DfSQ4uTDmJ-QRx322T89xQty_eH9z-2n6urrx8_bt1eVk7qeKinR1-WvGlqjQLc70SntvKyddztmNOPCSOlF12ihOAijDG_ErvOas9YwEBfk1Tq3LL6dfZ7sELLzfY-jj3O2WhtpAEwBX_4D7uOcynPZclk0brQSqlCwUi7FnJPv7CGFAdPRArOLIXY1xBZD7GKIXXqenybPu8G39x0nBwpweQIwO-y7orAL-S_HgTW80cuJfOVyKY03Pt2f-L_tL9amDqPFm1QGX__gRRkGyigpmPgDiY-skA</recordid><startdate>20090201</startdate><enddate>20090201</enddate><creator>Strube, Christina</creator><creator>Buschbaum, Sandra</creator><creator>Schnieder, Thomas</creator><general>Berlin/Heidelberg : Springer-Verlag</general><general>Springer-Verlag</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20090201</creationdate><title>Molecular characterization and real-time PCR transcriptional analysis of Dictyocaulus viviparus major sperm proteins</title><author>Strube, Christina ; Buschbaum, Sandra ; Schnieder, Thomas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c467t-44ae722871d8516db3f56ce47cecb086023844e3f9635213858293bfe620d8013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>3' Untranslated Regions</topic><topic>5' Untranslated Regions</topic><topic>Amino Acid Sequence</topic><topic>Amino acid substitution</topic><topic>Amino Acid Substitution - genetics</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cattle</topic><topic>Classical pathway</topic><topic>Complementary DNA</topic><topic>Computational Biology</topic><topic>Conserved sequence</topic><topic>Dictyocaulus - chemistry</topic><topic>Dictyocaulus - genetics</topic><topic>Dictyocaulus viviparus</topic><topic>DNA, Complementary</topic><topic>DNA, Helminth - chemistry</topic><topic>DNA, Helminth - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Profiling</topic><topic>General aspects</topic><topic>General aspects and techniques. Study of several systematic groups. Models</topic><topic>Genomics</topic><topic>Helminth Proteins - biosynthesis</topic><topic>Helminth Proteins - genetics</topic><topic>Immunology</topic><topic>Introns</topic><topic>Invertebrates</topic><topic>Isoforms</topic><topic>Medical Microbiology</topic><topic>Microbiology</topic><topic>Models, Molecular</topic><topic>Molecular Sequence Data</topic><topic>Nucleotide sequence</topic><topic>Original Paper</topic><topic>Polyadenylation</topic><topic>Polymerase chain reaction</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Protein Folding</topic><topic>Protein Isoforms - genetics</topic><topic>Protein Structure, Tertiary</topic><topic>Sequence Alignment</topic><topic>Sequence Analysis, DNA</topic><topic>Sperm</topic><topic>Transcription</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Strube, Christina</creatorcontrib><creatorcontrib>Buschbaum, Sandra</creatorcontrib><creatorcontrib>Schnieder, Thomas</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Parasitology research (1987)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Strube, Christina</au><au>Buschbaum, Sandra</au><au>Schnieder, Thomas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular characterization and real-time PCR transcriptional analysis of Dictyocaulus viviparus major sperm proteins</atitle><jtitle>Parasitology research (1987)</jtitle><stitle>Parasitol Res</stitle><addtitle>Parasitol Res</addtitle><date>2009-02-01</date><risdate>2009</risdate><volume>104</volume><issue>3</issue><spage>543</spage><epage>551</epage><pages>543-551</pages><issn>0932-0113</issn><eissn>1432-1955</eissn><coden>PARREZ</coden><abstract>Major sperm proteins (MSPs) represent a protein family occurring in nematodes only. Identification of the 3' and 5' untranslated region (UTR) completed the so far partial msp complementary DNA sequences of the bovine lungworm Dictyocaulus viviparus. The full-length transcript contains sequence tracts consistent with the Kozak and polyadenylation consensus sequence. On genomic level, three full-length sequences differing in three nucleotides were determined containing a 65-bp phase zero intron. Conceptual translation inferred two MSP isoforms due to one substitution within the 126-amino acid polypeptide. Bioinformatic analysis predicted that bovine lungworm MSP folds into an immunoglobulin-like seven-stranded beta sandwich as known for Caenorhabditis elegans and Ascaris suum. Furthermore, bovine lungworm MSP is confidentially predicted to be N-terminal-acetylated and secreted via a non-classical pathway. Quantitative real-time polymerase chain reaction analysis using ten developmental lungworm stages showed that msp is transcribed mainly in adult male parasites and in some degree in hypobiotic L5. However, marginal msp transcription was detectable in all of the investigated developmental lungworm stages.</abstract><cop>Berlin/Heidelberg</cop><pub>Berlin/Heidelberg : Springer-Verlag</pub><pmid>18853187</pmid><doi>10.1007/s00436-008-1228-5</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Parasitology research (1987), 2009-02, Vol.104 (3), p.543-551 |
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| subjects | 3' Untranslated Regions 5' Untranslated Regions Amino Acid Sequence Amino acid substitution Amino Acid Substitution - genetics Animals Biological and medical sciences Biomedical and Life Sciences Biomedicine Cattle Classical pathway Complementary DNA Computational Biology Conserved sequence Dictyocaulus - chemistry Dictyocaulus - genetics Dictyocaulus viviparus DNA, Complementary DNA, Helminth - chemistry DNA, Helminth - genetics Fundamental and applied biological sciences. Psychology Gene Expression Profiling General aspects General aspects and techniques. Study of several systematic groups. Models Genomics Helminth Proteins - biosynthesis Helminth Proteins - genetics Immunology Introns Invertebrates Isoforms Medical Microbiology Microbiology Models, Molecular Molecular Sequence Data Nucleotide sequence Original Paper Polyadenylation Polymerase chain reaction Polymerase Chain Reaction - methods Protein Folding Protein Isoforms - genetics Protein Structure, Tertiary Sequence Alignment Sequence Analysis, DNA Sperm Transcription |
| title | Molecular characterization and real-time PCR transcriptional analysis of Dictyocaulus viviparus major sperm proteins |
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