Genotyping of celiac disease-related-risk haplotypes using a closed-tube polymerase chain reaction analysis of dried blood and saliva disk samples

Expansion of molecular diagnostics more widely into clinical routines requires simplified methods allowing automation. We developed a homogeneous, multilabel polymerase chain reaction (PCR) method based on time-resolved fluorometry, and studied the use of dried disk samples in PCR. Celiac disease-re...

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Veröffentlicht in:	Analytical biochemistry 2009-03, Vol.386 (1), p.20-29
Hauptverfasser:	Ollikka, Pia, Raussi, Hanna-Mari, Laitala, Ville, Jaakkola, Lassi, Hovinen, Jari, Hemmilä, Ilkka, Ylikoski, Alice
Format:	Artikel
Sprache:	eng
Schlagworte:	Blood Blood disk Celiac Disease - genetics Genotype Haplotypes HLA-DQ alpha-Chains HLA-DQ Antigens - genetics HLA-DQ beta-Chains HLA-DQA1 05 HLA-DQB1 02 HLA-DQB1 0302 Homogeneous PCR Humans Methods Polymerase Chain Reaction - methods Research Design S&S 903 sample collection paper Saliva - chemistry Saliva disk Time-resolved fluorometry
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