Comparative proteomic analysis of trophozoites versus cysts of Giardia lamblia
The proteome of Giardia lamblia at its cyst stage was compared with that of trophozoites by using two-dimensional SDS-PAGE gel electrophoresis. Protein spots that increased in the extracts of cysts compared to trophozoites were identified by MALDI-TOF mass spectroscopy and categorized as cytoskeleta...
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| Veröffentlicht in: | Parasitology research (1987) 2009-01, Vol.104 (2), p.475-479 |
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| container_title | Parasitology research (1987) |
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| creator | Kim, Juri Bae, Sung-Su Sung, Moon-Hee Lee, Kyu-Ho Park, Soon-Jung |
| description | The proteome of Giardia lamblia at its cyst stage was compared with that of trophozoites by using two-dimensional SDS-PAGE gel electrophoresis. Protein spots that increased in the extracts of cysts compared to trophozoites were identified by MALDI-TOF mass spectroscopy and categorized as cytoskeletal proteins, metabolic enzymes, a cell-cycle-specific kinase, stress resistance proteins, and a protein involved in translation. Expression patterns of five of the identified proteins were examined during encystation by real-time PCR. Expression of cwp1 (encoding cyst wall protein 1), a marker for encystation, was increased 11-fold. In contrast, tim (encoding triose-1-phosphate isomerase) was expressed constitutively during encystation, and its transcription level was therefore used as a mRNA loading control. Expression of three genes encoding β-tubulin, vacuolar ATPase, and never-in-mitosis-A-related protein kinase did not vary significantly during encystation. Interestingly, genes encoding two heat shock proteins (Hsp70 and Hsp90) showed increased expression during encystation, suggesting that this differentiation process accompanies a cellular response to stress in G. lamblia. |
| doi_str_mv | 10.1007/s00436-008-1223-x |
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Protein spots that increased in the extracts of cysts compared to trophozoites were identified by MALDI-TOF mass spectroscopy and categorized as cytoskeletal proteins, metabolic enzymes, a cell-cycle-specific kinase, stress resistance proteins, and a protein involved in translation. Expression patterns of five of the identified proteins were examined during encystation by real-time PCR. Expression of cwp1 (encoding cyst wall protein 1), a marker for encystation, was increased 11-fold. In contrast, tim (encoding triose-1-phosphate isomerase) was expressed constitutively during encystation, and its transcription level was therefore used as a mRNA loading control. Expression of three genes encoding β-tubulin, vacuolar ATPase, and never-in-mitosis-A-related protein kinase did not vary significantly during encystation. Interestingly, genes encoding two heat shock proteins (Hsp70 and Hsp90) showed increased expression during encystation, suggesting that this differentiation process accompanies a cellular response to stress in G. lamblia.</description><identifier>ISSN: 0932-0113</identifier><identifier>EISSN: 1432-1955</identifier><identifier>DOI: 10.1007/s00436-008-1223-x</identifier><identifier>PMID: 18853189</identifier><identifier>CODEN: PARREZ</identifier><language>eng</language><publisher>Berlin/Heidelberg: Berlin/Heidelberg : Springer-Verlag</publisher><subject>Animals ; Biological and medical sciences ; Biomedical and Life Sciences ; Biomedicine ; Cysts - chemistry ; Electrophoresis, Gel, Two-Dimensional ; Fundamental and applied biological sciences. Psychology ; Gene Expression Profiling ; General aspects ; General aspects and techniques. Study of several systematic groups. Models ; Giardia lamblia - chemistry ; Immunology ; Invertebrates ; Medical Microbiology ; Microbiology ; Polymerase Chain Reaction - methods ; Proteome - analysis ; Protozoan Proteins - analysis ; Short Communication ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Trophozoites - chemistry</subject><ispartof>Parasitology research (1987), 2009-01, Vol.104 (2), p.475-479</ispartof><rights>Springer-Verlag 2008</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c462t-7652401053971127d8ff4cce9e3fc62c5b50babca6044bd60301a912908d01023</citedby><cites>FETCH-LOGICAL-c462t-7652401053971127d8ff4cce9e3fc62c5b50babca6044bd60301a912908d01023</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00436-008-1223-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00436-008-1223-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21071554$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18853189$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Juri</creatorcontrib><creatorcontrib>Bae, Sung-Su</creatorcontrib><creatorcontrib>Sung, Moon-Hee</creatorcontrib><creatorcontrib>Lee, Kyu-Ho</creatorcontrib><creatorcontrib>Park, Soon-Jung</creatorcontrib><title>Comparative proteomic analysis of trophozoites versus cysts of Giardia lamblia</title><title>Parasitology research (1987)</title><addtitle>Parasitol Res</addtitle><addtitle>Parasitol Res</addtitle><description>The proteome of Giardia lamblia at its cyst stage was compared with that of trophozoites by using two-dimensional SDS-PAGE gel electrophoresis. Protein spots that increased in the extracts of cysts compared to trophozoites were identified by MALDI-TOF mass spectroscopy and categorized as cytoskeletal proteins, metabolic enzymes, a cell-cycle-specific kinase, stress resistance proteins, and a protein involved in translation. Expression patterns of five of the identified proteins were examined during encystation by real-time PCR. Expression of cwp1 (encoding cyst wall protein 1), a marker for encystation, was increased 11-fold. In contrast, tim (encoding triose-1-phosphate isomerase) was expressed constitutively during encystation, and its transcription level was therefore used as a mRNA loading control. Expression of three genes encoding β-tubulin, vacuolar ATPase, and never-in-mitosis-A-related protein kinase did not vary significantly during encystation. Interestingly, genes encoding two heat shock proteins (Hsp70 and Hsp90) showed increased expression during encystation, suggesting that this differentiation process accompanies a cellular response to stress in G. lamblia.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cysts - chemistry</subject><subject>Electrophoresis, Gel, Two-Dimensional</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Profiling</subject><subject>General aspects</subject><subject>General aspects and techniques. Study of several systematic groups. Models</subject><subject>Giardia lamblia - chemistry</subject><subject>Immunology</subject><subject>Invertebrates</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Proteome - analysis</subject><subject>Protozoan Proteins - analysis</subject><subject>Short Communication</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><subject>Trophozoites - chemistry</subject><issn>0932-0113</issn><issn>1432-1955</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1v1DAQhi0EokvhB3CBXOAWmPFHPo5oVVqkCg7QszVxnOIqWS-epOry6_GSVblxGkvzvO9YjxCvET4gQP2RAbSqSoCmRClV-fBEbFArWWJrzFOxgTa_AVGdiRfMdwBYV1o_F2fYNEZh027E122c9pRoDve-2Kc4-zgFV9COxgMHLuJQzCnuf8bfMcyei3ufeOHCHXj-u7wMlPpAxUhTNwZ6KZ4NNLJ_dZrn4ubzxY_tVXn97fLL9tN16XQl57KujNSAYFRbI8q6b4ZBO-dbrwZXSWc6Ax11jirQuusrUIDUomyh6XNMqnPxfu3NX_61eJ7tFNj5caSdjwvbqmr0sTiDuIIuRebkB7tPYaJ0sAj2KNGuEm2WaI8S7UPOvDmVL93k-3-Jk7UMvDsBxI7GIdHOBX7kJEKNxujMyZXjvNrd-mTv4pKyWv7v9bdraKBo6Tbl4pvvEjArMI3RrVR_ADXckx0</recordid><startdate>20090101</startdate><enddate>20090101</enddate><creator>Kim, Juri</creator><creator>Bae, Sung-Su</creator><creator>Sung, Moon-Hee</creator><creator>Lee, Kyu-Ho</creator><creator>Park, Soon-Jung</creator><general>Berlin/Heidelberg : Springer-Verlag</general><general>Springer-Verlag</general><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20090101</creationdate><title>Comparative proteomic analysis of trophozoites versus cysts of Giardia lamblia</title><author>Kim, Juri ; Bae, Sung-Su ; Sung, Moon-Hee ; Lee, Kyu-Ho ; Park, Soon-Jung</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c462t-7652401053971127d8ff4cce9e3fc62c5b50babca6044bd60301a912908d01023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cysts - chemistry</topic><topic>Electrophoresis, Gel, Two-Dimensional</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Profiling</topic><topic>General aspects</topic><topic>General aspects and techniques. Study of several systematic groups. Models</topic><topic>Giardia lamblia - chemistry</topic><topic>Immunology</topic><topic>Invertebrates</topic><topic>Medical Microbiology</topic><topic>Microbiology</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Proteome - analysis</topic><topic>Protozoan Proteins - analysis</topic><topic>Short Communication</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</topic><topic>Trophozoites - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Juri</creatorcontrib><creatorcontrib>Bae, Sung-Su</creatorcontrib><creatorcontrib>Sung, Moon-Hee</creatorcontrib><creatorcontrib>Lee, Kyu-Ho</creatorcontrib><creatorcontrib>Park, Soon-Jung</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Parasitology research (1987)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Juri</au><au>Bae, Sung-Su</au><au>Sung, Moon-Hee</au><au>Lee, Kyu-Ho</au><au>Park, Soon-Jung</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative proteomic analysis of trophozoites versus cysts of Giardia lamblia</atitle><jtitle>Parasitology research (1987)</jtitle><stitle>Parasitol Res</stitle><addtitle>Parasitol Res</addtitle><date>2009-01-01</date><risdate>2009</risdate><volume>104</volume><issue>2</issue><spage>475</spage><epage>479</epage><pages>475-479</pages><issn>0932-0113</issn><eissn>1432-1955</eissn><coden>PARREZ</coden><abstract>The proteome of Giardia lamblia at its cyst stage was compared with that of trophozoites by using two-dimensional SDS-PAGE gel electrophoresis. Protein spots that increased in the extracts of cysts compared to trophozoites were identified by MALDI-TOF mass spectroscopy and categorized as cytoskeletal proteins, metabolic enzymes, a cell-cycle-specific kinase, stress resistance proteins, and a protein involved in translation. Expression patterns of five of the identified proteins were examined during encystation by real-time PCR. Expression of cwp1 (encoding cyst wall protein 1), a marker for encystation, was increased 11-fold. In contrast, tim (encoding triose-1-phosphate isomerase) was expressed constitutively during encystation, and its transcription level was therefore used as a mRNA loading control. Expression of three genes encoding β-tubulin, vacuolar ATPase, and never-in-mitosis-A-related protein kinase did not vary significantly during encystation. Interestingly, genes encoding two heat shock proteins (Hsp70 and Hsp90) showed increased expression during encystation, suggesting that this differentiation process accompanies a cellular response to stress in G. lamblia.</abstract><cop>Berlin/Heidelberg</cop><pub>Berlin/Heidelberg : Springer-Verlag</pub><pmid>18853189</pmid><doi>10.1007/s00436-008-1223-x</doi><tpages>5</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences Biomedical and Life Sciences Biomedicine Cysts - chemistry Electrophoresis, Gel, Two-Dimensional Fundamental and applied biological sciences. Psychology Gene Expression Profiling General aspects General aspects and techniques. Study of several systematic groups. Models Giardia lamblia - chemistry Immunology Invertebrates Medical Microbiology Microbiology Polymerase Chain Reaction - methods Proteome - analysis Protozoan Proteins - analysis Short Communication Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Trophozoites - chemistry |
| title | Comparative proteomic analysis of trophozoites versus cysts of Giardia lamblia |
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