Identification of cytochrome P450 enzymes in human colorectal metastases and the surrounding liver: a proteomic approach
We describe the direct identification of multiple cytochrome P450 (CYP) enzymes in healthy and cancerous tissue. CYPs in human liver colorectal metastases were compared with those in the surrounding liver using a mass spectrometry-based proteomic approach. Nano-scale reversed phase liquid chromatogr...
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| Veröffentlicht in: | European journal of cancer (1990) 2004-09, Vol.40 (14), p.2127-2134 |
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| container_title | European journal of cancer (1990) |
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| creator | Lane, C.S. Nisar, S. Griffiths, W.J. Fuller, B.J. Davidson, B.R. Hewes, J. Welham, K.J. Patterson, L.H. |
| description | We describe the direct identification of multiple cytochrome P450 (CYP) enzymes in healthy and cancerous tissue. CYPs in human liver colorectal metastases were compared with those in the surrounding liver using a mass spectrometry-based proteomic approach. Nano-scale reversed phase liquid chromatography combined with electrospray ionisation tandem mass spectrometry has been used to identify CYPs with no pre-selection of the proteins anticipated. Fourteen distinct CYP enzymes from the subfamilies 1A, 2A, 2B, 2C, 2D, 2E, 3A, 4A, 4F, 8B and 27A were positively identified; 13 in the liver samples and 12 in the tumour tissue. It was found that three of the colorectal metastases expressed essentially the same drug-metabolising pattern of CYPs as the surrounding liver, whilst three tumour samples from different individuals showed no CYP expression. This was likely in at least one case to be due to low sample mass. The CYP expression profile in an individual tumour is likely to be an important determinant in predicting the outcome of cancer chemotherapy. |
| doi_str_mv | 10.1016/j.ejca.2004.04.029 |
| format | Article |
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CYPs in human liver colorectal metastases were compared with those in the surrounding liver using a mass spectrometry-based proteomic approach. Nano-scale reversed phase liquid chromatography combined with electrospray ionisation tandem mass spectrometry has been used to identify CYPs with no pre-selection of the proteins anticipated. Fourteen distinct CYP enzymes from the subfamilies 1A, 2A, 2B, 2C, 2D, 2E, 3A, 4A, 4F, 8B and 27A were positively identified; 13 in the liver samples and 12 in the tumour tissue. It was found that three of the colorectal metastases expressed essentially the same drug-metabolising pattern of CYPs as the surrounding liver, whilst three tumour samples from different individuals showed no CYP expression. This was likely in at least one case to be due to low sample mass. The CYP expression profile in an individual tumour is likely to be an important determinant in predicting the outcome of cancer chemotherapy.</description><identifier>ISSN: 0959-8049</identifier><identifier>EISSN: 1879-0852</identifier><identifier>DOI: 10.1016/j.ejca.2004.04.029</identifier><identifier>PMID: 15341988</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Adult ; Aged ; Biological and medical sciences ; Chemotherapy ; Colorectal metastasis ; Colorectal Neoplasms - enzymology ; Colorectal Neoplasms - secondary ; Cytochrome P-450 Enzyme System - metabolism ; Cytochrome P450 ; Electrophoresis, Polyacrylamide Gel ; Electrospray ionisation ; Female ; Human liver ; Humans ; Liver Neoplasms - enzymology ; Male ; Medical sciences ; Microsomes, Liver - enzymology ; Middle Aged ; Pharmacology. Drug treatments ; Proteome - metabolism ; Proteomics ; Tandem mass spectrometry ; Tumors</subject><ispartof>European journal of cancer (1990), 2004-09, Vol.40 (14), p.2127-2134</ispartof><rights>2004 Elsevier Ltd</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c407t-32b5c3c317b63ab05d714842f27d62fff0f93017eeb0fc2d7a6e233aa059118b3</citedby><cites>FETCH-LOGICAL-c407t-32b5c3c317b63ab05d714842f27d62fff0f93017eeb0fc2d7a6e233aa059118b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0959804904003995$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16147974$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15341988$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lane, C.S.</creatorcontrib><creatorcontrib>Nisar, S.</creatorcontrib><creatorcontrib>Griffiths, W.J.</creatorcontrib><creatorcontrib>Fuller, B.J.</creatorcontrib><creatorcontrib>Davidson, B.R.</creatorcontrib><creatorcontrib>Hewes, J.</creatorcontrib><creatorcontrib>Welham, K.J.</creatorcontrib><creatorcontrib>Patterson, L.H.</creatorcontrib><title>Identification of cytochrome P450 enzymes in human colorectal metastases and the surrounding liver: a proteomic approach</title><title>European journal of cancer (1990)</title><addtitle>Eur J Cancer</addtitle><description>We describe the direct identification of multiple cytochrome P450 (CYP) enzymes in healthy and cancerous tissue. CYPs in human liver colorectal metastases were compared with those in the surrounding liver using a mass spectrometry-based proteomic approach. Nano-scale reversed phase liquid chromatography combined with electrospray ionisation tandem mass spectrometry has been used to identify CYPs with no pre-selection of the proteins anticipated. Fourteen distinct CYP enzymes from the subfamilies 1A, 2A, 2B, 2C, 2D, 2E, 3A, 4A, 4F, 8B and 27A were positively identified; 13 in the liver samples and 12 in the tumour tissue. It was found that three of the colorectal metastases expressed essentially the same drug-metabolising pattern of CYPs as the surrounding liver, whilst three tumour samples from different individuals showed no CYP expression. This was likely in at least one case to be due to low sample mass. The CYP expression profile in an individual tumour is likely to be an important determinant in predicting the outcome of cancer chemotherapy.</description><subject>Adult</subject><subject>Aged</subject><subject>Biological and medical sciences</subject><subject>Chemotherapy</subject><subject>Colorectal metastasis</subject><subject>Colorectal Neoplasms - enzymology</subject><subject>Colorectal Neoplasms - secondary</subject><subject>Cytochrome P-450 Enzyme System - metabolism</subject><subject>Cytochrome P450</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Electrospray ionisation</subject><subject>Female</subject><subject>Human liver</subject><subject>Humans</subject><subject>Liver Neoplasms - enzymology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Microsomes, Liver - enzymology</subject><subject>Middle Aged</subject><subject>Pharmacology. Drug treatments</subject><subject>Proteome - metabolism</subject><subject>Proteomics</subject><subject>Tandem mass spectrometry</subject><subject>Tumors</subject><issn>0959-8049</issn><issn>1879-0852</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1r3DAQhkVpaLZp_0APRZfm5o0ky5ZVegmhH4FAe2jPYiyPulpsaSvZIdtfX5ldyK3wwgzomRnxEPKOsy1nvL3Zb3FvYSsYk9s1Qr8gG94pXbGuES_JhulGVx2T-pK8znnPGFOdZK_IJW9qyXXXbcjT_YBh9s5bmH0MNDpqj3O0uxQnpD9kwyiGv8cJM_WB7pYJArVxjAntDCOdcIZcUp4hDHTeIc1LSnEJgw-_6egfMX2kQA8pzhgnbykcSg9294ZcOBgzvj3XK_Lry-efd9-qh-9f7-9uHyormZqrWvSNrW3NVd_W0LNmUFx2UjihhlY455jTNeMKsWfOikFBi6KuAVijOe_6-opcn_aWs38WzLOZfLY4jhAwLtm0bSeLzaaA4gTaFHNO6Mwh-QnS0XBmVt9mb1bfZvVt1ghdht6fty_9hMPzyFlwAT6cAcgWRpcgWJ-fuZZLpZUs3KcTh8XFo8dksvUYLA5-VW2G6P_3j38FG5_h</recordid><startdate>20040901</startdate><enddate>20040901</enddate><creator>Lane, C.S.</creator><creator>Nisar, S.</creator><creator>Griffiths, W.J.</creator><creator>Fuller, B.J.</creator><creator>Davidson, B.R.</creator><creator>Hewes, J.</creator><creator>Welham, K.J.</creator><creator>Patterson, L.H.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20040901</creationdate><title>Identification of cytochrome P450 enzymes in human colorectal metastases and the surrounding liver: a proteomic approach</title><author>Lane, C.S. ; Nisar, S. ; Griffiths, W.J. ; Fuller, B.J. ; Davidson, B.R. ; Hewes, J. ; Welham, K.J. ; Patterson, L.H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c407t-32b5c3c317b63ab05d714842f27d62fff0f93017eeb0fc2d7a6e233aa059118b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Biological and medical sciences</topic><topic>Chemotherapy</topic><topic>Colorectal metastasis</topic><topic>Colorectal Neoplasms - enzymology</topic><topic>Colorectal Neoplasms - secondary</topic><topic>Cytochrome P-450 Enzyme System - metabolism</topic><topic>Cytochrome P450</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Electrospray ionisation</topic><topic>Female</topic><topic>Human liver</topic><topic>Humans</topic><topic>Liver Neoplasms - enzymology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Microsomes, Liver - enzymology</topic><topic>Middle Aged</topic><topic>Pharmacology. Drug treatments</topic><topic>Proteome - metabolism</topic><topic>Proteomics</topic><topic>Tandem mass spectrometry</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lane, C.S.</creatorcontrib><creatorcontrib>Nisar, S.</creatorcontrib><creatorcontrib>Griffiths, W.J.</creatorcontrib><creatorcontrib>Fuller, B.J.</creatorcontrib><creatorcontrib>Davidson, B.R.</creatorcontrib><creatorcontrib>Hewes, J.</creatorcontrib><creatorcontrib>Welham, K.J.</creatorcontrib><creatorcontrib>Patterson, L.H.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of cancer (1990)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lane, C.S.</au><au>Nisar, S.</au><au>Griffiths, W.J.</au><au>Fuller, B.J.</au><au>Davidson, B.R.</au><au>Hewes, J.</au><au>Welham, K.J.</au><au>Patterson, L.H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of cytochrome P450 enzymes in human colorectal metastases and the surrounding liver: a proteomic approach</atitle><jtitle>European journal of cancer (1990)</jtitle><addtitle>Eur J Cancer</addtitle><date>2004-09-01</date><risdate>2004</risdate><volume>40</volume><issue>14</issue><spage>2127</spage><epage>2134</epage><pages>2127-2134</pages><issn>0959-8049</issn><eissn>1879-0852</eissn><abstract>We describe the direct identification of multiple cytochrome P450 (CYP) enzymes in healthy and cancerous tissue. CYPs in human liver colorectal metastases were compared with those in the surrounding liver using a mass spectrometry-based proteomic approach. Nano-scale reversed phase liquid chromatography combined with electrospray ionisation tandem mass spectrometry has been used to identify CYPs with no pre-selection of the proteins anticipated. Fourteen distinct CYP enzymes from the subfamilies 1A, 2A, 2B, 2C, 2D, 2E, 3A, 4A, 4F, 8B and 27A were positively identified; 13 in the liver samples and 12 in the tumour tissue. It was found that three of the colorectal metastases expressed essentially the same drug-metabolising pattern of CYPs as the surrounding liver, whilst three tumour samples from different individuals showed no CYP expression. This was likely in at least one case to be due to low sample mass. The CYP expression profile in an individual tumour is likely to be an important determinant in predicting the outcome of cancer chemotherapy.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>15341988</pmid><doi>10.1016/j.ejca.2004.04.029</doi><tpages>8</tpages></addata></record> |
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| subjects | Adult Aged Biological and medical sciences Chemotherapy Colorectal metastasis Colorectal Neoplasms - enzymology Colorectal Neoplasms - secondary Cytochrome P-450 Enzyme System - metabolism Cytochrome P450 Electrophoresis, Polyacrylamide Gel Electrospray ionisation Female Human liver Humans Liver Neoplasms - enzymology Male Medical sciences Microsomes, Liver - enzymology Middle Aged Pharmacology. Drug treatments Proteome - metabolism Proteomics Tandem mass spectrometry Tumors |
| title | Identification of cytochrome P450 enzymes in human colorectal metastases and the surrounding liver: a proteomic approach |
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