$Isolation of a Highly Clonogenic and Multipotential Subfraction of Adult Stem Cells from Bone Marrow Stroma$

Isolation of a Highly Clonogenic and Multipotential Subfraction of Adult Stem Cells from Bone Marrow Stroma

Attempts have been made to develop cell and gene therapies using the adult stem cells from bone marrow referred to as mesenchymal stem cells or marrow stromal cells (MSCs). However, the results have been variable in part because there are no standardized protocols for preparing and characterizing MS...

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Veröffentlicht in:	Stem cells (Dayton, Ohio) Ohio), 2004-09, Vol.22 (5), p.823-831
Hauptverfasser:	Smith, Jason R., Pochampally, Radhika, Perry, Anthony, Hsu, Shu‐Ching, Prockop, Darwin J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adipocytes Adipocytes - cytology Adipocytes - immunology Bone Marrow Cells - cytology Bone Marrow Cells - drug effects Bone Marrow Cells - immunology Bone Marrow Transplantation - methods Cell Culture Techniques - methods Cell Differentiation - drug effects Cell Differentiation - immunology Cell Lineage - drug effects Cell Lineage - immunology Cell Proliferation - drug effects Cell Separation - methods Cells, Cultured Clone Cells - cytology Clone Cells - drug effects Clone Cells - immunology Colony-Forming Units Assay - methods Culture Media - pharmacology Gene Expression Regulation - drug effects Gene Expression Regulation - genetics Humans Mineralizing cells MSCs Multipotent Stem Cells - cytology Multipotent Stem Cells - drug effects Multipotent Stem Cells - immunology Osteoblasts - cytology Osteoblasts - immunology RNA, Messenger - analysis RNA, Messenger - genetics RS cells Stem Cell Transplantation - methods Stromal Cells - cytology Stromal Cells - drug effects Stromal Cells - immunology
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