GFAPdelta immunostaining improves visualization of normal and pathologic astrocytic heterogeneity
Neuropathological analysis of cellular mechanisms underlying gliosis and brain tumors is slowed by the lack of markers allowing to distinguish glial subpopulations in normal or pathological human brains. We therefore evaluated GFAPdelta immunostaining in a wide panel of astrogliosis and gliomas, and...
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| Veröffentlicht in: | Neuropathology 2009-02, Vol.29 (1), p.31-39 |
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| creator | Andreiuolo, Felipe Junier, Marie-Pierre Hol, Elly M Miquel, Catherine Chimelli, Leila Leonard, Nadine Chneiweiss, Hervé Daumas-Duport, Catherine Varlet, Pascale |
| description | Neuropathological analysis of cellular mechanisms underlying gliosis and brain tumors is slowed by the lack of markers allowing to distinguish glial subpopulations in normal or pathological human brains. We therefore evaluated GFAPdelta immunostaining in a wide panel of astrogliosis and gliomas, and compared these with GFAP and vimentin. In normal tissue, gliosis and gliomas, GFAPdelta immunostaining was observed in astrocytes with relatively high GFAP levels. GFAPdelta immunostaining was most conspicuous in glia limitans astrocytes. In Chaslin's gliosis accompanying chronic epilepsy, GFAPdelta immunostaining evidenced the glia limitans reactive astrocytes as the source of the dense fibrillary meshwork typical of Chaslin's gliosis. Interestingly GFAPdelta and vimentin immunostainings coincided in normal tissues and gliosis, but not in gliomas. Altogether these results show that combined GFAP, GFAPdelta and vimentin labelling reveals fine gliofilament regulation in normal and pathological brain. |
| doi_str_mv | 10.1111/j.1440-1789.2008.00936.x |
| format | Article |
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| ispartof | Neuropathology, 2009-02, Vol.29 (1), p.31-39 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Adolescent Adult Aged Astrocytes - chemistry Astrocytes - cytology Astrocytes - pathology Brain - cytology Brain - pathology Brain Chemistry Brain Diseases - metabolism Brain Diseases - pathology Brain Neoplasms - chemistry Brain Neoplasms - pathology Epilepsy - metabolism Epilepsy - pathology Female Glial Fibrillary Acidic Protein - analysis Glioma - chemistry Glioma - pathology Gliosis - metabolism Gliosis - pathology Hippocampus - chemistry Hippocampus - pathology Humans Immunohistochemistry - methods Male Microscopy, Confocal Middle Aged Sclerosis - metabolism Sclerosis - pathology Vimentin - analysis Young Adult |
| title | GFAPdelta immunostaining improves visualization of normal and pathologic astrocytic heterogeneity |
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