A Sensitive and Robust Competitive Enzyme-Linked Immunosorbent Assay for Brazil Nut (Bertholletia excelsa L.) Detection

Undeclared Brazil nut residue in food products is of great concern because it can trigger life-threatening allergic reactions in sensitive patients. A rabbit polyclonal antibody-based competitive ELISA (IC50 = 23.2 ± 9 ng/mL, n = 76) with good sensitivity, detection range of 10−90 ng/mL, was develop...

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Veröffentlicht in:	Journal of agricultural and food chemistry 2009-01, Vol.57 (2), p.769-776
Hauptverfasser:	Sharma, Girdhari M, Roux, Kenneth H, Sathe, Shridhar K
Format:	Artikel
Sprache:	eng
Schlagworte:	allergens Animals Antigens, Plant - analysis Antigens, Plant - immunology Bertholletia excelsa Biological and medical sciences Brazil Brazil nuts competitive enzyme-linked immunosorbent assay enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods food analysis Food Hypersensitivity - immunology Food industries Fruit and vegetable industries Fundamental and applied biological sciences. Psychology General aspects Humans Methods of analysis, processing and quality control, regulation, standards Nuts - chemistry Plant Proteins - analysis Plant Proteins - immunology polyclonal antibodies Rabbits Sensitivity and Specificity Toxicology in Agriculture and Food
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