Reversible Control of Exo- and Endo-Budding Transitions in a Photosensitive Lipid Membrane
We have developed a method for the photomanipulation of lipid membrane morphology in which the shape of a vesicle can be switched by light through the use of a synthetic photosensitive amphiphile containing an azobenzene unit (KAON12). We prepared cell-sized liposomes from KAON12 and 1,2-dioleoyl-sn...
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| Veröffentlicht in: | Chembiochem : a European journal of chemical biology 2009-01, Vol.10 (2), p.251-256 |
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| container_title | Chembiochem : a European journal of chemical biology |
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| creator | Ishii, Ken-ichi Hamada, Tsutomu Hatakeyama, Masaomi Sugimoto, Ryoko Nagasaki, Takeshi Takagi, Masahiro |
| description | We have developed a method for the photomanipulation of lipid membrane morphology in which the shape of a vesicle can be switched by light through the use of a synthetic photosensitive amphiphile containing an azobenzene unit (KAON12). We prepared cell-sized liposomes from KAON12 and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and conducted real-time observations of vesicular transformation in the photosensitive liposome by phase-contrast microscopy. Budding transitions--either budding toward the centre of the liposome (endo-bud) or budding out of the liposome (exo-bud)--could be controlled by light. We discuss the mechanism of this transformation in terms of the change in the effective membrane surface area due to photoisomerization of the constituent molecules. |
| doi_str_mv | 10.1002/cbic.200800482 |
| format | Article |
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We discuss the mechanism of this transformation in terms of the change in the effective membrane surface area due to photoisomerization of the constituent molecules.</description><identifier>ISSN: 1439-4227</identifier><identifier>EISSN: 1439-7633</identifier><identifier>DOI: 10.1002/cbic.200800482</identifier><identifier>PMID: 19132694</identifier><language>eng</language><publisher>Weinheim: Wiley-VCH Verlag</publisher><subject>azobenzenes ; budding ; Endocytosis - radiation effects ; Exocytosis - radiation effects ; Isomerism ; Light ; liposomes ; Liposomes - chemistry ; Liposomes - radiation effects ; membranes ; Microscopy, Phase-Contrast ; Osmotic Pressure - radiation effects ; Phospholipids - chemistry ; photocontrol</subject><ispartof>Chembiochem : a European journal of chemical biology, 2009-01, Vol.10 (2), p.251-256</ispartof><rights>Copyright © 2009 WILEY‐VCH Verlag GmbH & Co. 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We discuss the mechanism of this transformation in terms of the change in the effective membrane surface area due to photoisomerization of the constituent molecules.</description><subject>azobenzenes</subject><subject>budding</subject><subject>Endocytosis - radiation effects</subject><subject>Exocytosis - radiation effects</subject><subject>Isomerism</subject><subject>Light</subject><subject>liposomes</subject><subject>Liposomes - chemistry</subject><subject>Liposomes - radiation effects</subject><subject>membranes</subject><subject>Microscopy, Phase-Contrast</subject><subject>Osmotic Pressure - radiation effects</subject><subject>Phospholipids - chemistry</subject><subject>photocontrol</subject><issn>1439-4227</issn><issn>1439-7633</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1PGzEQhi1UBOHjyrH1iduG8cd6d4_NKtBIQSA-Llwsrz1LjTbr1E5S-PcsTUR762lGo-d9NXoIOWMwZgD8wjbejjlACSBLvkdGTIoqK5QQX3a75Lw4JEcpvQBApQQ7IIesYoKrSo7I0x1uMCbfdEjr0K9i6Gho6fQ1ZNT0jk57F7LJ2jnfP9OHaPrkVz70ifqeGnr7M6xCwj_HDdK5X3pHr3HRDCCekP3WdAlPd_OYPF5OH-of2fzmalZ_n2dWCsGzPHfAhEIlihbASswLRGddw2XbOjRFUanG2JZJXjqbg-KKcVMaJptSoqrEMTnf9i5j-LXGtNILnyx23fBDWCetVCkEsGIAx1vQxpBSxFYvo1-Y-KYZ6A-b-sOm_rQ5BL7umtfNAt1ffKdvAKot8Nt3-PafOl1PZvW_5d-22dYEbZ6jT_rxng8qgOVlXnAu3gGsVIo1</recordid><startdate>20090126</startdate><enddate>20090126</enddate><creator>Ishii, Ken-ichi</creator><creator>Hamada, Tsutomu</creator><creator>Hatakeyama, Masaomi</creator><creator>Sugimoto, Ryoko</creator><creator>Nagasaki, Takeshi</creator><creator>Takagi, Masahiro</creator><general>Wiley-VCH Verlag</general><general>WILEY‐VCH Verlag</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20090126</creationdate><title>Reversible Control of Exo- and Endo-Budding Transitions in a Photosensitive Lipid Membrane</title><author>Ishii, Ken-ichi ; Hamada, Tsutomu ; Hatakeyama, Masaomi ; Sugimoto, Ryoko ; Nagasaki, Takeshi ; Takagi, Masahiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4332-55d0136e637f00c4e57eedcdb24ffdea7796bacf1428dc5062612a8a14b84e693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>azobenzenes</topic><topic>budding</topic><topic>Endocytosis - radiation effects</topic><topic>Exocytosis - radiation effects</topic><topic>Isomerism</topic><topic>Light</topic><topic>liposomes</topic><topic>Liposomes - chemistry</topic><topic>Liposomes - radiation effects</topic><topic>membranes</topic><topic>Microscopy, Phase-Contrast</topic><topic>Osmotic Pressure - radiation effects</topic><topic>Phospholipids - chemistry</topic><topic>photocontrol</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ishii, Ken-ichi</creatorcontrib><creatorcontrib>Hamada, Tsutomu</creatorcontrib><creatorcontrib>Hatakeyama, Masaomi</creatorcontrib><creatorcontrib>Sugimoto, Ryoko</creatorcontrib><creatorcontrib>Nagasaki, Takeshi</creatorcontrib><creatorcontrib>Takagi, Masahiro</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Chembiochem : a European journal of chemical biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ishii, Ken-ichi</au><au>Hamada, Tsutomu</au><au>Hatakeyama, Masaomi</au><au>Sugimoto, Ryoko</au><au>Nagasaki, Takeshi</au><au>Takagi, Masahiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Reversible Control of Exo- and Endo-Budding Transitions in a Photosensitive Lipid Membrane</atitle><jtitle>Chembiochem : a European journal of chemical biology</jtitle><addtitle>Chembiochem</addtitle><date>2009-01-26</date><risdate>2009</risdate><volume>10</volume><issue>2</issue><spage>251</spage><epage>256</epage><pages>251-256</pages><issn>1439-4227</issn><eissn>1439-7633</eissn><abstract>We have developed a method for the photomanipulation of lipid membrane morphology in which the shape of a vesicle can be switched by light through the use of a synthetic photosensitive amphiphile containing an azobenzene unit (KAON12). 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| ispartof | Chembiochem : a European journal of chemical biology, 2009-01, Vol.10 (2), p.251-256 |
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| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | azobenzenes budding Endocytosis - radiation effects Exocytosis - radiation effects Isomerism Light liposomes Liposomes - chemistry Liposomes - radiation effects membranes Microscopy, Phase-Contrast Osmotic Pressure - radiation effects Phospholipids - chemistry photocontrol |
| title | Reversible Control of Exo- and Endo-Budding Transitions in a Photosensitive Lipid Membrane |
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