Expression of methionine aminopeptidase 2, N-myristoyltransferase, and N-myristoyltransferase inhibitor protein 71 in HT29
Protein myristoylation is a co-translational process, catalyzed by N-myristoyltransferase (NMT) that occurs after the initiating methionine is removed by methionine aminopeptidase (MetAP). The enzymes NMT and MetAP play a major role in the process of myristoylation of oncoproteins including the c-sr...
Gespeichert in:
| Veröffentlicht in: | Biochemical and biophysical research communications 2004-09, Vol.322 (3), p.1012-1017 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1017 |
|---|---|
| container_issue | 3 |
| container_start_page | 1012 |
| container_title | Biochemical and biophysical research communications |
| container_volume | 322 |
| creator | Selvakumar, Ponniah Lakshmikuttyamma, Ashakumary Lawman, Zoe Bonham, Keith Dimmock, Jonathan R. Sharma, Rajendra K. |
| description | Protein myristoylation is a co-translational process, catalyzed by
N-myristoyltransferase (NMT) that occurs after the initiating methionine is removed by methionine aminopeptidase (MetAP). The enzymes NMT and MetAP play a major role in the process of myristoylation of oncoproteins including the c-src family. In this study, we examined the levels of expression of MetAP2, NMT, and NMT inhibitor protein 71 (NIP71) in human colon cancer cell lines (HCCLs). We examined the influence of cell density on the expression of the above proteins in HT29 cells. Western blot analysis of MetAP2 and NMT demonstrated higher levels of protein expression in low density of HT29 while low expression in high density was observed. In addition, we observed that NIP71 and pp60
c-src expressions were dependent on the cell density of HT29. This is the first study demonstrating the expression of MetAP2, NMT, pp60
c-src, and NIP71 in HCCLs. |
| doi_str_mv | 10.1016/j.bbrc.2004.08.021 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_66825897</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0006291X04017498</els_id><sourcerecordid>66825897</sourcerecordid><originalsourceid>FETCH-LOGICAL-c352t-53b893c18eda5c96c53df1a29360b6301f1f98e5aaa2424b4affe1d3e82a1f443</originalsourceid><addsrcrecordid>eNp9kEGLFDEQhYMo7rj6BzxITp6220rSyXbAiyyrKyx6WcFbSKcrbIbppE0y4vjrzTADnvRURb1Xj6qPkNcMegZMvdv205RdzwGGHsYeOHtCNgw0dJzB8JRsAEB1XLPvF-RFKVsAxgaln5MLJoVQUskN-X37a81YSkiRJk8XrI-tDRGpXUJMK641zLYg5Vf0S7cccig1HXY121g85qZcURvnf2g0xMcwhZoyXXOqGCK9Zm1I7x64fkmeebsr-OpcL8m3j7cPN3fd_ddPn28-3HdOSF47KaZRC8dGnK10WjkpZs8s10LBpAQwz7weUVpr-cCHabDeI5sFjtwyPwzikrw95bYTfuyxVLOE4nC3sxHTvhilRi5Hfd2M_GR0OZWS0Zs1h8Xmg2FgjsTN1hyJmyNxA6NpxNvSm3P6flpw_rtyRtwM708GbD_-DJhNcQGjwzlkdNXMKfwv_w_DuZQn</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>66825897</pqid></control><display><type>article</type><title>Expression of methionine aminopeptidase 2, N-myristoyltransferase, and N-myristoyltransferase inhibitor protein 71 in HT29</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Selvakumar, Ponniah ; Lakshmikuttyamma, Ashakumary ; Lawman, Zoe ; Bonham, Keith ; Dimmock, Jonathan R. ; Sharma, Rajendra K.</creator><creatorcontrib>Selvakumar, Ponniah ; Lakshmikuttyamma, Ashakumary ; Lawman, Zoe ; Bonham, Keith ; Dimmock, Jonathan R. ; Sharma, Rajendra K.</creatorcontrib><description>Protein myristoylation is a co-translational process, catalyzed by
N-myristoyltransferase (NMT) that occurs after the initiating methionine is removed by methionine aminopeptidase (MetAP). The enzymes NMT and MetAP play a major role in the process of myristoylation of oncoproteins including the c-src family. In this study, we examined the levels of expression of MetAP2, NMT, and NMT inhibitor protein 71 (NIP71) in human colon cancer cell lines (HCCLs). We examined the influence of cell density on the expression of the above proteins in HT29 cells. Western blot analysis of MetAP2 and NMT demonstrated higher levels of protein expression in low density of HT29 while low expression in high density was observed. In addition, we observed that NIP71 and pp60
c-src expressions were dependent on the cell density of HT29. This is the first study demonstrating the expression of MetAP2, NMT, pp60
c-src, and NIP71 in HCCLs.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2004.08.021</identifier><identifier>PMID: 15336565</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Acyltransferases - genetics ; Aminopeptidases - genetics ; Cell Line, Tumor ; Colonic Neoplasms ; Enzyme Inhibitors - metabolism ; Gene Expression Regulation, Enzymologic - genetics ; Gene Expression Regulation, Neoplastic - genetics ; Humans ; Lipid modification ; Metalloendopeptidases - genetics ; Methionine aminopeptidase 2 ; Myristoylation ; N-Myristoyltransferase ; N-Myristoyltransferase inhibitor protein ; Neoplasm Proteins - genetics ; pp60 c-src</subject><ispartof>Biochemical and biophysical research communications, 2004-09, Vol.322 (3), p.1012-1017</ispartof><rights>2004 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c352t-53b893c18eda5c96c53df1a29360b6301f1f98e5aaa2424b4affe1d3e82a1f443</citedby><cites>FETCH-LOGICAL-c352t-53b893c18eda5c96c53df1a29360b6301f1f98e5aaa2424b4affe1d3e82a1f443</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2004.08.021$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15336565$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Selvakumar, Ponniah</creatorcontrib><creatorcontrib>Lakshmikuttyamma, Ashakumary</creatorcontrib><creatorcontrib>Lawman, Zoe</creatorcontrib><creatorcontrib>Bonham, Keith</creatorcontrib><creatorcontrib>Dimmock, Jonathan R.</creatorcontrib><creatorcontrib>Sharma, Rajendra K.</creatorcontrib><title>Expression of methionine aminopeptidase 2, N-myristoyltransferase, and N-myristoyltransferase inhibitor protein 71 in HT29</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Protein myristoylation is a co-translational process, catalyzed by
N-myristoyltransferase (NMT) that occurs after the initiating methionine is removed by methionine aminopeptidase (MetAP). The enzymes NMT and MetAP play a major role in the process of myristoylation of oncoproteins including the c-src family. In this study, we examined the levels of expression of MetAP2, NMT, and NMT inhibitor protein 71 (NIP71) in human colon cancer cell lines (HCCLs). We examined the influence of cell density on the expression of the above proteins in HT29 cells. Western blot analysis of MetAP2 and NMT demonstrated higher levels of protein expression in low density of HT29 while low expression in high density was observed. In addition, we observed that NIP71 and pp60
c-src expressions were dependent on the cell density of HT29. This is the first study demonstrating the expression of MetAP2, NMT, pp60
c-src, and NIP71 in HCCLs.</description><subject>Acyltransferases - genetics</subject><subject>Aminopeptidases - genetics</subject><subject>Cell Line, Tumor</subject><subject>Colonic Neoplasms</subject><subject>Enzyme Inhibitors - metabolism</subject><subject>Gene Expression Regulation, Enzymologic - genetics</subject><subject>Gene Expression Regulation, Neoplastic - genetics</subject><subject>Humans</subject><subject>Lipid modification</subject><subject>Metalloendopeptidases - genetics</subject><subject>Methionine aminopeptidase 2</subject><subject>Myristoylation</subject><subject>N-Myristoyltransferase</subject><subject>N-Myristoyltransferase inhibitor protein</subject><subject>Neoplasm Proteins - genetics</subject><subject>pp60 c-src</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEGLFDEQhYMo7rj6BzxITp6220rSyXbAiyyrKyx6WcFbSKcrbIbppE0y4vjrzTADnvRURb1Xj6qPkNcMegZMvdv205RdzwGGHsYeOHtCNgw0dJzB8JRsAEB1XLPvF-RFKVsAxgaln5MLJoVQUskN-X37a81YSkiRJk8XrI-tDRGpXUJMK641zLYg5Vf0S7cccig1HXY121g85qZcURvnf2g0xMcwhZoyXXOqGCK9Zm1I7x64fkmeebsr-OpcL8m3j7cPN3fd_ddPn28-3HdOSF47KaZRC8dGnK10WjkpZs8s10LBpAQwz7weUVpr-cCHabDeI5sFjtwyPwzikrw95bYTfuyxVLOE4nC3sxHTvhilRi5Hfd2M_GR0OZWS0Zs1h8Xmg2FgjsTN1hyJmyNxA6NpxNvSm3P6flpw_rtyRtwM708GbD_-DJhNcQGjwzlkdNXMKfwv_w_DuZQn</recordid><startdate>20040924</startdate><enddate>20040924</enddate><creator>Selvakumar, Ponniah</creator><creator>Lakshmikuttyamma, Ashakumary</creator><creator>Lawman, Zoe</creator><creator>Bonham, Keith</creator><creator>Dimmock, Jonathan R.</creator><creator>Sharma, Rajendra K.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20040924</creationdate><title>Expression of methionine aminopeptidase 2, N-myristoyltransferase, and N-myristoyltransferase inhibitor protein 71 in HT29</title><author>Selvakumar, Ponniah ; Lakshmikuttyamma, Ashakumary ; Lawman, Zoe ; Bonham, Keith ; Dimmock, Jonathan R. ; Sharma, Rajendra K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c352t-53b893c18eda5c96c53df1a29360b6301f1f98e5aaa2424b4affe1d3e82a1f443</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Acyltransferases - genetics</topic><topic>Aminopeptidases - genetics</topic><topic>Cell Line, Tumor</topic><topic>Colonic Neoplasms</topic><topic>Enzyme Inhibitors - metabolism</topic><topic>Gene Expression Regulation, Enzymologic - genetics</topic><topic>Gene Expression Regulation, Neoplastic - genetics</topic><topic>Humans</topic><topic>Lipid modification</topic><topic>Metalloendopeptidases - genetics</topic><topic>Methionine aminopeptidase 2</topic><topic>Myristoylation</topic><topic>N-Myristoyltransferase</topic><topic>N-Myristoyltransferase inhibitor protein</topic><topic>Neoplasm Proteins - genetics</topic><topic>pp60 c-src</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Selvakumar, Ponniah</creatorcontrib><creatorcontrib>Lakshmikuttyamma, Ashakumary</creatorcontrib><creatorcontrib>Lawman, Zoe</creatorcontrib><creatorcontrib>Bonham, Keith</creatorcontrib><creatorcontrib>Dimmock, Jonathan R.</creatorcontrib><creatorcontrib>Sharma, Rajendra K.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Selvakumar, Ponniah</au><au>Lakshmikuttyamma, Ashakumary</au><au>Lawman, Zoe</au><au>Bonham, Keith</au><au>Dimmock, Jonathan R.</au><au>Sharma, Rajendra K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of methionine aminopeptidase 2, N-myristoyltransferase, and N-myristoyltransferase inhibitor protein 71 in HT29</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2004-09-24</date><risdate>2004</risdate><volume>322</volume><issue>3</issue><spage>1012</spage><epage>1017</epage><pages>1012-1017</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Protein myristoylation is a co-translational process, catalyzed by
N-myristoyltransferase (NMT) that occurs after the initiating methionine is removed by methionine aminopeptidase (MetAP). The enzymes NMT and MetAP play a major role in the process of myristoylation of oncoproteins including the c-src family. In this study, we examined the levels of expression of MetAP2, NMT, and NMT inhibitor protein 71 (NIP71) in human colon cancer cell lines (HCCLs). We examined the influence of cell density on the expression of the above proteins in HT29 cells. Western blot analysis of MetAP2 and NMT demonstrated higher levels of protein expression in low density of HT29 while low expression in high density was observed. In addition, we observed that NIP71 and pp60
c-src expressions were dependent on the cell density of HT29. This is the first study demonstrating the expression of MetAP2, NMT, pp60
c-src, and NIP71 in HCCLs.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>15336565</pmid><doi>10.1016/j.bbrc.2004.08.021</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-291X |
| ispartof | Biochemical and biophysical research communications, 2004-09, Vol.322 (3), p.1012-1017 |
| issn | 0006-291X 1090-2104 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_66825897 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Acyltransferases - genetics Aminopeptidases - genetics Cell Line, Tumor Colonic Neoplasms Enzyme Inhibitors - metabolism Gene Expression Regulation, Enzymologic - genetics Gene Expression Regulation, Neoplastic - genetics Humans Lipid modification Metalloendopeptidases - genetics Methionine aminopeptidase 2 Myristoylation N-Myristoyltransferase N-Myristoyltransferase inhibitor protein Neoplasm Proteins - genetics pp60 c-src |
| title | Expression of methionine aminopeptidase 2, N-myristoyltransferase, and N-myristoyltransferase inhibitor protein 71 in HT29 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T16%3A26%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Expression%20of%20methionine%20aminopeptidase%202,%20N-myristoyltransferase,%20and%20N-myristoyltransferase%20inhibitor%20protein%2071%20in%20HT29&rft.jtitle=Biochemical%20and%20biophysical%20research%20communications&rft.au=Selvakumar,%20Ponniah&rft.date=2004-09-24&rft.volume=322&rft.issue=3&rft.spage=1012&rft.epage=1017&rft.pages=1012-1017&rft.issn=0006-291X&rft.eissn=1090-2104&rft_id=info:doi/10.1016/j.bbrc.2004.08.021&rft_dat=%3Cproquest_cross%3E66825897%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=66825897&rft_id=info:pmid/15336565&rft_els_id=S0006291X04017498&rfr_iscdi=true |