A protein-based oxygen biosensor for high-throughput monitoring of cell growth and cell viability
Fluorescently labeled hemocyanin has been previously proposed as an oxygen sensor. In this study, we explored the efficacy of this biosensor for monitoring the biological oxygen consumption of bacteria and its use in testing bacterial cell growth and viability of Escherichia coli, Pseudomonas aerugi...
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| Veröffentlicht in: | Analytical biochemistry 2009-02, Vol.385 (2), p.242-248 |
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| container_title | Analytical biochemistry |
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| creator | Strianese, Maria Zauner, Gerhild Tepper, Armand W.J.W. Bubacco, Luigi Breukink, Eefjan Aartsma, Thijs J. Canters, Gerard W. Tabares, Leandro C. |
| description | Fluorescently labeled hemocyanin has been previously proposed as an oxygen sensor. In this study, we explored the efficacy of this biosensor for monitoring the biological oxygen consumption of bacteria and its use in testing bacterial cell growth and viability of
Escherichia coli, Pseudomonas aeruginosa, Paracoccus denitrificans, and
Staphylococcus simulans. Using a microwell plate, the time courses for the complete deoxygenation of samples with different initial concentrations of cells were obtained and the doubling times were extracted. The applicability of our fluorescence-based cell growth assay as an antibacterial drug screening method was also explored. The results provide a proof-of-principle for a simple, quantitative, and sensitive method for high-throughput monitoring of prokaryotic cell growth and antibiotic susceptibility screening. |
| doi_str_mv | 10.1016/j.ab.2008.11.017 |
| format | Article |
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Escherichia coli, Pseudomonas aeruginosa, Paracoccus denitrificans, and
Staphylococcus simulans. Using a microwell plate, the time courses for the complete deoxygenation of samples with different initial concentrations of cells were obtained and the doubling times were extracted. The applicability of our fluorescence-based cell growth assay as an antibacterial drug screening method was also explored. The results provide a proof-of-principle for a simple, quantitative, and sensitive method for high-throughput monitoring of prokaryotic cell growth and antibiotic susceptibility screening.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/j.ab.2008.11.017</identifier><identifier>PMID: 19084497</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Anti-Bacterial Agents - pharmacology ; Antibiotic screening ; Bacteria - cytology ; Bacteria - growth & development ; Biosensing Techniques - methods ; Biosensors ; Cell viability ; Escherichia coli ; Escherichia coli - cytology ; Escherichia coli - growth & development ; FRET ; Hemocyanins ; Microbial Sensitivity Tests ; Oxygen - analysis ; Oxygen sensing ; Paracoccus denitrificans ; Paracoccus denitrificans - cytology ; Paracoccus denitrificans - growth & development ; Pseudomonas aeruginosa ; Pseudomonas aeruginosa - cytology ; Pseudomonas aeruginosa - growth & development ; Staphylococcus - cytology ; Staphylococcus - growth & development ; Staphylococcus simulans</subject><ispartof>Analytical biochemistry, 2009-02, Vol.385 (2), p.242-248</ispartof><rights>2008 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c379t-e48f27e1d92c75d3db9e07524344572c5cc9b812b2a3d705799f0226ec84d6543</citedby><cites>FETCH-LOGICAL-c379t-e48f27e1d92c75d3db9e07524344572c5cc9b812b2a3d705799f0226ec84d6543</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ab.2008.11.017$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19084497$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Strianese, Maria</creatorcontrib><creatorcontrib>Zauner, Gerhild</creatorcontrib><creatorcontrib>Tepper, Armand W.J.W.</creatorcontrib><creatorcontrib>Bubacco, Luigi</creatorcontrib><creatorcontrib>Breukink, Eefjan</creatorcontrib><creatorcontrib>Aartsma, Thijs J.</creatorcontrib><creatorcontrib>Canters, Gerard W.</creatorcontrib><creatorcontrib>Tabares, Leandro C.</creatorcontrib><title>A protein-based oxygen biosensor for high-throughput monitoring of cell growth and cell viability</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>Fluorescently labeled hemocyanin has been previously proposed as an oxygen sensor. In this study, we explored the efficacy of this biosensor for monitoring the biological oxygen consumption of bacteria and its use in testing bacterial cell growth and viability of
Escherichia coli, Pseudomonas aeruginosa, Paracoccus denitrificans, and
Staphylococcus simulans. Using a microwell plate, the time courses for the complete deoxygenation of samples with different initial concentrations of cells were obtained and the doubling times were extracted. The applicability of our fluorescence-based cell growth assay as an antibacterial drug screening method was also explored. The results provide a proof-of-principle for a simple, quantitative, and sensitive method for high-throughput monitoring of prokaryotic cell growth and antibiotic susceptibility screening.</description><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotic screening</subject><subject>Bacteria - cytology</subject><subject>Bacteria - growth & development</subject><subject>Biosensing Techniques - methods</subject><subject>Biosensors</subject><subject>Cell viability</subject><subject>Escherichia coli</subject><subject>Escherichia coli - cytology</subject><subject>Escherichia coli - growth & development</subject><subject>FRET</subject><subject>Hemocyanins</subject><subject>Microbial Sensitivity Tests</subject><subject>Oxygen - analysis</subject><subject>Oxygen sensing</subject><subject>Paracoccus denitrificans</subject><subject>Paracoccus denitrificans - cytology</subject><subject>Paracoccus denitrificans - growth & development</subject><subject>Pseudomonas aeruginosa</subject><subject>Pseudomonas aeruginosa - cytology</subject><subject>Pseudomonas aeruginosa - growth & development</subject><subject>Staphylococcus - cytology</subject><subject>Staphylococcus - growth & development</subject><subject>Staphylococcus simulans</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtr3DAQh0VpaLZJ7z0VnXqzO3rYsnoLIX1AoJfkLPQY21p2ra1kp93_vl52oafSwzAMfPNj5iPkPYOaAWs_bWvrag7Q1YzVwNQrsmGg2woE6NdkAwCi4q1W1-RtKVsAxmTTviHXTEMnpVYbYu_oIacZ41Q5WzDQ9Ps44ERdTAWnkjLt1xrjMFbzmNMyjIdlpvs0xTnlOA009dTjbkeHnH7NI7VTOM8v0bq4i_Pxllz1dlfw3aXfkOcvD0_336rHH1-_3989Vl4oPVcou54rZEFzr5oggtMIquFSSNko7hvvtesYd9yKoKBRWvfAeYu-k6FtpLghH8-56z8_Fyyz2cdyOsVOmJZi2rZbw0D8F-QrI6A5gXAGfU6lZOzNIce9zUfDwJz8m62xzpz8G8bM6n9d-XDJXtwew9-Fi_AV-HwGcFXxEjGb4iNOHkPM6GcTUvx3-h9uJpUA</recordid><startdate>20090215</startdate><enddate>20090215</enddate><creator>Strianese, Maria</creator><creator>Zauner, Gerhild</creator><creator>Tepper, Armand W.J.W.</creator><creator>Bubacco, Luigi</creator><creator>Breukink, Eefjan</creator><creator>Aartsma, Thijs J.</creator><creator>Canters, Gerard W.</creator><creator>Tabares, Leandro C.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20090215</creationdate><title>A protein-based oxygen biosensor for high-throughput monitoring of cell growth and cell viability</title><author>Strianese, Maria ; Zauner, Gerhild ; Tepper, Armand W.J.W. ; Bubacco, Luigi ; Breukink, Eefjan ; Aartsma, Thijs J. ; Canters, Gerard W. ; Tabares, Leandro C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c379t-e48f27e1d92c75d3db9e07524344572c5cc9b812b2a3d705799f0226ec84d6543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibiotic screening</topic><topic>Bacteria - cytology</topic><topic>Bacteria - growth & development</topic><topic>Biosensing Techniques - methods</topic><topic>Biosensors</topic><topic>Cell viability</topic><topic>Escherichia coli</topic><topic>Escherichia coli - cytology</topic><topic>Escherichia coli - growth & development</topic><topic>FRET</topic><topic>Hemocyanins</topic><topic>Microbial Sensitivity Tests</topic><topic>Oxygen - analysis</topic><topic>Oxygen sensing</topic><topic>Paracoccus denitrificans</topic><topic>Paracoccus denitrificans - cytology</topic><topic>Paracoccus denitrificans - growth & development</topic><topic>Pseudomonas aeruginosa</topic><topic>Pseudomonas aeruginosa - cytology</topic><topic>Pseudomonas aeruginosa - growth & development</topic><topic>Staphylococcus - cytology</topic><topic>Staphylococcus - growth & development</topic><topic>Staphylococcus simulans</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Strianese, Maria</creatorcontrib><creatorcontrib>Zauner, Gerhild</creatorcontrib><creatorcontrib>Tepper, Armand W.J.W.</creatorcontrib><creatorcontrib>Bubacco, Luigi</creatorcontrib><creatorcontrib>Breukink, Eefjan</creatorcontrib><creatorcontrib>Aartsma, Thijs J.</creatorcontrib><creatorcontrib>Canters, Gerard W.</creatorcontrib><creatorcontrib>Tabares, Leandro C.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Strianese, Maria</au><au>Zauner, Gerhild</au><au>Tepper, Armand W.J.W.</au><au>Bubacco, Luigi</au><au>Breukink, Eefjan</au><au>Aartsma, Thijs J.</au><au>Canters, Gerard W.</au><au>Tabares, Leandro C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A protein-based oxygen biosensor for high-throughput monitoring of cell growth and cell viability</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>2009-02-15</date><risdate>2009</risdate><volume>385</volume><issue>2</issue><spage>242</spage><epage>248</epage><pages>242-248</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>Fluorescently labeled hemocyanin has been previously proposed as an oxygen sensor. In this study, we explored the efficacy of this biosensor for monitoring the biological oxygen consumption of bacteria and its use in testing bacterial cell growth and viability of
Escherichia coli, Pseudomonas aeruginosa, Paracoccus denitrificans, and
Staphylococcus simulans. Using a microwell plate, the time courses for the complete deoxygenation of samples with different initial concentrations of cells were obtained and the doubling times were extracted. The applicability of our fluorescence-based cell growth assay as an antibacterial drug screening method was also explored. The results provide a proof-of-principle for a simple, quantitative, and sensitive method for high-throughput monitoring of prokaryotic cell growth and antibiotic susceptibility screening.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>19084497</pmid><doi>10.1016/j.ab.2008.11.017</doi><tpages>7</tpages></addata></record> |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Anti-Bacterial Agents - pharmacology Antibiotic screening Bacteria - cytology Bacteria - growth & development Biosensing Techniques - methods Biosensors Cell viability Escherichia coli Escherichia coli - cytology Escherichia coli - growth & development FRET Hemocyanins Microbial Sensitivity Tests Oxygen - analysis Oxygen sensing Paracoccus denitrificans Paracoccus denitrificans - cytology Paracoccus denitrificans - growth & development Pseudomonas aeruginosa Pseudomonas aeruginosa - cytology Pseudomonas aeruginosa - growth & development Staphylococcus - cytology Staphylococcus - growth & development Staphylococcus simulans |
| title | A protein-based oxygen biosensor for high-throughput monitoring of cell growth and cell viability |
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