Angiopoietin-1 reduces vascular endothelial growth factor-induced brain endothelial permeability via upregulation of ZO-2
Brain microvessels possess barrier structures comprising tight junctions which are critical for the maintenance of central nervous system homeostasis. Brain vascular diseases, such as ischemic stroke damage to blood-brain barrier, increase the vascular permeability, and then lead to vasogenic brain...
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| Veröffentlicht in: | International journal of molecular medicine 2009-02, Vol.23 (2), p.279-284 |
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| container_title | International journal of molecular medicine |
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| creator | Lee, Sae-Won Kim, Woo Jun, Hyoung-Oh Choi, Yoon Kim, Kyu-Won |
| description | Brain microvessels possess barrier structures comprising tight junctions
which are critical for the maintenance of central nervous system homeostasis.
Brain vascular diseases, such as ischemic stroke damage to blood-brain barrier,
increase the vascular permeability, and then lead to vasogenic brain edema. Herein,
we examined whether angiopoietin-1 (Ang-1) could regulate zonula occludens-2 (ZO-2)
expression and counteract vascular endothelial growth factor (VEGF)-induced vascular
permeability. When we treated brain microvascular endothelial cells with Ang-1,
Ang-1 caused a time- and dose-dependent increase of ZO-2 and down-regulation in
endothelial permeability. VEGF, one of the key regulators of ischemia-induced
vascular permeability, increased endothelial cell permeability in vitro, whereas,
Ang-1 reversed this VEGF effect by up-regulating ZO-2 expression. Additionally,
the recovery effect of Ang-1 on permeability was strongly blocked by siRNA against
ZO-2. Collectively, our results suggest that Ang-1 shows anti-permeability activity
through up-regulation of ZO-2. |
| doi_str_mv | 10.3892/ijmm_00000128 |
| format | Article |
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which are critical for the maintenance of central nervous system homeostasis.
Brain vascular diseases, such as ischemic stroke damage to blood-brain barrier,
increase the vascular permeability, and then lead to vasogenic brain edema. Herein,
we examined whether angiopoietin-1 (Ang-1) could regulate zonula occludens-2 (ZO-2)
expression and counteract vascular endothelial growth factor (VEGF)-induced vascular
permeability. When we treated brain microvascular endothelial cells with Ang-1,
Ang-1 caused a time- and dose-dependent increase of ZO-2 and down-regulation in
endothelial permeability. VEGF, one of the key regulators of ischemia-induced
vascular permeability, increased endothelial cell permeability in vitro, whereas,
Ang-1 reversed this VEGF effect by up-regulating ZO-2 expression. Additionally,
the recovery effect of Ang-1 on permeability was strongly blocked by siRNA against
ZO-2. Collectively, our results suggest that Ang-1 shows anti-permeability activity
through up-regulation of ZO-2.</description><identifier>ISSN: 1107-3756</identifier><identifier>DOI: 10.3892/ijmm_00000128</identifier><identifier>PMID: 19148554</identifier><language>eng</language><publisher>Greece: D.A. Spandidos</publisher><subject>Angiopoietin-1 - pharmacology ; Blood-Brain Barrier - metabolism ; Brain - blood supply ; Brain - metabolism ; Capillary Permeability ; Endothelial Cells - drug effects ; Endothelial Cells - metabolism ; Endothelium, Vascular - cytology ; Endothelium, Vascular - metabolism ; Humans ; Membrane Proteins - genetics ; Membrane Proteins - metabolism ; RNA, Small Interfering - genetics ; RNA, Small Interfering - metabolism ; Up-Regulation ; Vascular Endothelial Growth Factor A - metabolism ; Zonula Occludens-2 Protein</subject><ispartof>International journal of molecular medicine, 2009-02, Vol.23 (2), p.279-284</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c366t-be8343277215a9fe3ff804dd8ee9a5a479895d5db1de0e40846324b74795769f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,5556,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19148554$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Sae-Won</creatorcontrib><creatorcontrib>Kim, Woo</creatorcontrib><creatorcontrib>Jun, Hyoung-Oh</creatorcontrib><creatorcontrib>Choi, Yoon</creatorcontrib><creatorcontrib>Kim, Kyu-Won</creatorcontrib><title>Angiopoietin-1 reduces vascular endothelial growth factor-induced brain endothelial permeability via upregulation of ZO-2</title><title>International journal of molecular medicine</title><addtitle>Int J Mol Med</addtitle><description>Brain microvessels possess barrier structures comprising tight junctions
which are critical for the maintenance of central nervous system homeostasis.
Brain vascular diseases, such as ischemic stroke damage to blood-brain barrier,
increase the vascular permeability, and then lead to vasogenic brain edema. Herein,
we examined whether angiopoietin-1 (Ang-1) could regulate zonula occludens-2 (ZO-2)
expression and counteract vascular endothelial growth factor (VEGF)-induced vascular
permeability. When we treated brain microvascular endothelial cells with Ang-1,
Ang-1 caused a time- and dose-dependent increase of ZO-2 and down-regulation in
endothelial permeability. VEGF, one of the key regulators of ischemia-induced
vascular permeability, increased endothelial cell permeability in vitro, whereas,
Ang-1 reversed this VEGF effect by up-regulating ZO-2 expression. Additionally,
the recovery effect of Ang-1 on permeability was strongly blocked by siRNA against
ZO-2. Collectively, our results suggest that Ang-1 shows anti-permeability activity
through up-regulation of ZO-2.</description><subject>Angiopoietin-1 - pharmacology</subject><subject>Blood-Brain Barrier - metabolism</subject><subject>Brain - blood supply</subject><subject>Brain - metabolism</subject><subject>Capillary Permeability</subject><subject>Endothelial Cells - drug effects</subject><subject>Endothelial Cells - metabolism</subject><subject>Endothelium, Vascular - cytology</subject><subject>Endothelium, Vascular - metabolism</subject><subject>Humans</subject><subject>Membrane Proteins - genetics</subject><subject>Membrane Proteins - metabolism</subject><subject>RNA, Small Interfering - genetics</subject><subject>RNA, Small Interfering - metabolism</subject><subject>Up-Regulation</subject><subject>Vascular Endothelial Growth Factor A - metabolism</subject><subject>Zonula Occludens-2 Protein</subject><issn>1107-3756</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpV0T1PwzAQBmAPIAqFkRV5YkEBf8b2WCG-JCQWWFgiJ760Rkkc7ATUf0-qFlW95YZ79A7vIXRJyS3Xht35r7YtyGYo00folFKiMq5kPkNnKX0RwqQw-gTNqKFCSylO0XrRLX3og4fBdxnFEdxYQcI_NlVjYyOGzoVhBY23DV7G8DuscG2rIcTMdxvqcBmt7w5cD7EFW_rGD2v84y0e-wjLKW7wocOhxp9vGTtHx7VtElzs9hx9PD683z9nr29PL_eL16zieT5kJWguOFOKUWlNDbyuNRHOaQBjpRXKaCOddCV1QEAQLXLORKmmg1S5qfkcXW9z-xi-R0hD0fpUQdPYDsKYijzXjEulJphtYRVDShHqoo--tXFdUFJs-i0O-p381S54LFtwe70rdwI3W5B62znvQtqb_88wzpgyTAv-B2_xiBI</recordid><startdate>20090201</startdate><enddate>20090201</enddate><creator>Lee, Sae-Won</creator><creator>Kim, Woo</creator><creator>Jun, Hyoung-Oh</creator><creator>Choi, Yoon</creator><creator>Kim, Kyu-Won</creator><general>D.A. Spandidos</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20090201</creationdate><title>Angiopoietin-1 reduces vascular endothelial growth factor-induced brain endothelial permeability via upregulation of ZO-2</title><author>Lee, Sae-Won ; Kim, Woo ; Jun, Hyoung-Oh ; Choi, Yoon ; Kim, Kyu-Won</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c366t-be8343277215a9fe3ff804dd8ee9a5a479895d5db1de0e40846324b74795769f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Angiopoietin-1 - pharmacology</topic><topic>Blood-Brain Barrier - metabolism</topic><topic>Brain - blood supply</topic><topic>Brain - metabolism</topic><topic>Capillary Permeability</topic><topic>Endothelial Cells - drug effects</topic><topic>Endothelial Cells - metabolism</topic><topic>Endothelium, Vascular - cytology</topic><topic>Endothelium, Vascular - metabolism</topic><topic>Humans</topic><topic>Membrane Proteins - genetics</topic><topic>Membrane Proteins - metabolism</topic><topic>RNA, Small Interfering - genetics</topic><topic>RNA, Small Interfering - metabolism</topic><topic>Up-Regulation</topic><topic>Vascular Endothelial Growth Factor A - metabolism</topic><topic>Zonula Occludens-2 Protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Sae-Won</creatorcontrib><creatorcontrib>Kim, Woo</creatorcontrib><creatorcontrib>Jun, Hyoung-Oh</creatorcontrib><creatorcontrib>Choi, Yoon</creatorcontrib><creatorcontrib>Kim, Kyu-Won</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of molecular medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Sae-Won</au><au>Kim, Woo</au><au>Jun, Hyoung-Oh</au><au>Choi, Yoon</au><au>Kim, Kyu-Won</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Angiopoietin-1 reduces vascular endothelial growth factor-induced brain endothelial permeability via upregulation of ZO-2</atitle><jtitle>International journal of molecular medicine</jtitle><addtitle>Int J Mol Med</addtitle><date>2009-02-01</date><risdate>2009</risdate><volume>23</volume><issue>2</issue><spage>279</spage><epage>284</epage><pages>279-284</pages><issn>1107-3756</issn><abstract>Brain microvessels possess barrier structures comprising tight junctions
which are critical for the maintenance of central nervous system homeostasis.
Brain vascular diseases, such as ischemic stroke damage to blood-brain barrier,
increase the vascular permeability, and then lead to vasogenic brain edema. Herein,
we examined whether angiopoietin-1 (Ang-1) could regulate zonula occludens-2 (ZO-2)
expression and counteract vascular endothelial growth factor (VEGF)-induced vascular
permeability. When we treated brain microvascular endothelial cells with Ang-1,
Ang-1 caused a time- and dose-dependent increase of ZO-2 and down-regulation in
endothelial permeability. VEGF, one of the key regulators of ischemia-induced
vascular permeability, increased endothelial cell permeability in vitro, whereas,
Ang-1 reversed this VEGF effect by up-regulating ZO-2 expression. Additionally,
the recovery effect of Ang-1 on permeability was strongly blocked by siRNA against
ZO-2. Collectively, our results suggest that Ang-1 shows anti-permeability activity
through up-regulation of ZO-2.</abstract><cop>Greece</cop><pub>D.A. Spandidos</pub><pmid>19148554</pmid><doi>10.3892/ijmm_00000128</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | International journal of molecular medicine, 2009-02, Vol.23 (2), p.279-284 |
| issn | 1107-3756 |
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| source | Spandidos Publications Journals; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Angiopoietin-1 - pharmacology Blood-Brain Barrier - metabolism Brain - blood supply Brain - metabolism Capillary Permeability Endothelial Cells - drug effects Endothelial Cells - metabolism Endothelium, Vascular - cytology Endothelium, Vascular - metabolism Humans Membrane Proteins - genetics Membrane Proteins - metabolism RNA, Small Interfering - genetics RNA, Small Interfering - metabolism Up-Regulation Vascular Endothelial Growth Factor A - metabolism Zonula Occludens-2 Protein |
| title | Angiopoietin-1 reduces vascular endothelial growth factor-induced brain endothelial permeability via upregulation of ZO-2 |
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